LOCUS BBa_K228854 4378 bp DNA linear 22-Nov-2024 DEFINITION AND GATE Sal+SupD+LacP+RBS(B0032)+T7ptag ACCESSION BBa_K228854 VERSION BBa_K228854.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 RBS 209..221 /label=RBS-3\Weak RBS 215..218 /label=RBS misc_feature 209..221 /label=BBa_B0032 RBS 209..221 /label=BBa_B0032 CDS 228..2900 /label=cds CDS 228..2900 /label=BBa_K228000 stem_loop 2920..2963 /label=stem_loop misc_feature 2909..2988 /label=BBa_B0010 terminator 2909..2988 /label=BBa_B0010 stem_loop 3004..3023 /label=T7 TE misc_feature 3030..3030 /label=stop polyA_site 3024..3037 /label=polya misc_feature 2997..3037 /label=BBa_B0012 terminator 2997..3037 /label=BBa_B0012 CDS 3046..3948 /label=NahR promoter 3949..4038 /label=Pr promoter promoter 4027..4095 /label=pSal promoter 3046..4097 /label=BBa_K228004 misc_feature 4106..4241 /label=BBa_K228001 stem_loop 4261..4304 /label=stem_loop misc_feature 4250..4329 /label=BBa_B0010 terminator 4250..4329 /label=BBa_B0010 stem_loop 4345..4364 /label=T7 TE misc_feature 4371..4371 /label=stop polyA_site 4365..4378 /label=polya misc_feature 4338..4378 /label=BBa_B0012 terminator 4338..4378 /label=BBa_B0012 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagtc acacaggaaa gtactagatg accatgatta 241 ccgtgcacta gaataccatt aacattgcta agaacgactt ctctgacatc gaactggctg 301 ctatcccgtt caacactctg gctgaccatt acggtgagcg tttagctcgc gaacagttgg 361 cccttgagca tgagtcttac gagatgggtg aagcacgctt ccgcaagatg tttgagcgtc 421 aacttaaagc tggtgaggtt gcggataacg ctgccgccaa gcctctcatc actaccctac 481 tccctaagat gattgcacgc atcaacgact ggtttgagga agtgaaagct aagcgcggca 541 agcgcccgac agccttccag ttcctgtagg aaatcaagcc ggaagccgta gcgtacatca 601 ccattaagac cactctggct tgcctaacca gtgctgacaa tacaaccgtt caggctgtag 661 caagcgcaat cggtcgggcc attgaggacg aggctcgctt cggtcgtatc cgtgaccttg 721 aagctaagca cttcaagaaa aacgttgagg aacaactcaa caagcgcgta gggcacgtct 781 acaagaaagc atttatgcaa gttgtcgagg ctgacatgct ctctaagggt ctactcggtg 841 gcgaggcgtg gtcttcgtgg cataaggaag actctattca tgtaggagta cgctgcatcg 901 agatgctcat tgagtcaacc ggaatggtta gcttacaccg ccaaaatgct ggcgtagtag 961 gtcaagactc tgagactatc gaactcgcac ctgaatacgc tgaggctatc gcaacccgtg 1021 caggtgcgct ggctggcatc tctccgatgt tccaaccttg cgtagttcct cctaagccgt 1081 ggactggcat tactggtggt ggctattggg ctaacggtcg tcgtcctctg gcgctggtgc 1141 gtactcacag taagaaagca ctgatgcgct acgaagacgt ttacatgcct gaggtgtaca 1201 aagcgattaa cattgcgcaa aacaccgcat ggaaaatcaa caagaaagtc ctagcggtcg 1261 ccaacgtaat caccaagtgg aagcattgtc cggtcgagga catccctgcg attgagcgtg 1321 aagaactccc gatgaaaccg gaagacatcg acatgaatcc tgaggctctc accgcgtgga 1381 aacgtgctgc cgctgctgtg taccgcaagg acaaggctcg caagtctcgc cgtatcagcc 1441 ttgagttcat gcttgagcaa gccaataagt ttgctaacca taaggccatc tggttccctt 1501 acaacatgga ctggcgcggt cgtgtttacg ctgtgtcaat gttcaacccg caaggtaacg 1561 atatgaccaa aggactgctt acgctggcga aaggtaaacc aatcggtaag gaaggttact 1621 actggctgaa aatccacggt gcaaactgtg cgggtgtcga taaggttccg ttccctgagc 1681 gcatcaagtt cattgaggaa aaccacgaga acatcatggc ttgcgctaag tctccactgg 1741 agaacacttg gtgggctgag caagattctc cgttctgctt ccttgcgttc tgctttgagt 1801 acgctggggt acagcaccac ggcctgagct ataactgctc ccttccgctg gcgtttgacg 1861 ggtcttgctc tggcatccag cacttctccg cgatgctccg agatgaggta ggtggtcgcg 1921 cggttaactt gcttcctagt gaaaccgttc aggacatcta cgggattgtt gctaagaaag 1981 tcaacgagat tctacaagca gacgcaatca atgggaccga taacgaagta gttaccgtga 2041 ccgatgagaa cactggtgaa atctctgaga aagtcaagct gggcactaag gcactggctg 2101 gtcaatggct ggcttacggt gttactcgca gtgtgactaa gcgttcagtc atgacgctgg 2161 cttacgggtc caaagagttc ggcttccgtc aacaagtgct ggaagatacc attcagccag 2221 ctattgattc cggcaagggt ctgatgttca ctcagccgaa tcaggctgct ggatacatgg 2281 ctaagctgat ttgggaatct gtgagcgtga cggtggtagc tgcggttgaa gcaatgaact 2341 ggcttaagtc tgctgctaag ctgctggctg ctgaggtcaa agataagaag actggagaga 2401 ttcttcgcaa gcgttgcgct gtgcattggg taactcctga tggtttccct gtgtggcagg 2461 aatacaagaa gcctattcag acgcgcttga acctgatgtt cctcggtcag ttccgcttac 2521 agcctaccat taacaccaac aaagatagcg agattgatgc acacaaacag gagtctggta 2581 tcgctcctaa ctttgtacac agccaagacg gtagccacct tcgtaagact gtagtgtggg 2641 cacacgagaa gtacggaatc gaatcttttg cactgattca cgactccttc ggtaccattc 2701 cggctgacgc tgcgaacctg ttcaaagcag tgcgcgaaac tatggttgac acatatgagt 2761 cttgtgatgt actggctgat ttctacgacc agttcgctga ccagttgcac gagtctcaat 2821 tggacaaaat gccagcactt ccggctaaag gtaacttgaa cctccgtgac atcttagagt 2881 cggacttcgc gttcgcataa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg 2941 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac 3001 actggctcac cttcgggtgg gcctttctgc gtttatatac tagagtcaat ccgtaaacag 3061 gtcaaacatc agttgccgca accaaatatt ggctaggtcc ttgtggtact tcgcatgcca 3121 gaacatgttg atggctattt caggcaagac gactgggtgc ggcaaggcgc ttaggccgaa 3181 gggctccacg cagcagtcgg ctaaacgtat cggcacagtg gcgagcagat cggtgcgctg 3241 gaggatgtgg ccaacggcgg cgaagtgcgg cacttccaga cggatgtcgc gccggatgcc 3301 gacccgtgtc atgtacgtgt ccacctcgcc gtggccggtg ccagcggcga tgacacgcac 3361 gtggccgtag gaacagaagc gctccagagt caggggttcg cgggtgactg gatggtcctt 3421 gcgacatagg cacacgtagt gattctggag cagccggcgc tgaaagaagc cagtttgcag 3481 attgggaagc aggcccacgg ccaagtccac ggttccgttc tgcaaggcct gcatcaggct 3541 catcgaactg tcgcgcaccg tactgatcac gcaattgggg gcctggtgag ccagcacatc 3601 catcagccgc ggcatgaagt agatctcgcc aatgtcggtc atggccaggg tgaaggtacg 3661 ctcgctggtc agcggatcga agctttcatg gtgctgtagg gcgttgcgca gtgcgtgcat 3721 ggccgaagtg acgggctcgg ccagatgcgc ggcatagggt gtgggttcca ttccctgatg 3781 tgtgcgcacg aagagtgggt cctgtagcga ggtgcgcagg cgtttcagcg cattgctcac 3841 ggcaggctgg gtcaggccca ggttctccgc agtgatagag acgcgtctgt cgaccagcaa 3901 ctggttgaac accaccagca ggtttaaatc caggtcacgc agttccatgg ggcctcgctt 3961 gggttattgc tggtgcccgg ccgggcgcaa tattcatgtt gatgatttat tatatatcga 4021 gtggtgtatt tatcaatatt gtttgctccg ttatcgttat taacaagtca tcaataaagc 4081 catcacgagt accatagtac tagagcaatt cggagagatg ccggagcggc tgaacggacc 4141 ggtctctaaa accggagtag gggcaactct accgggggtt caaatccccc tctctccgcc 4201 actacagatc cttagcgaaa gctaaggatt ttttttaagc ttactagagc caggcatcaa 4261 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg 4321 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata //