LOCUS BBa_K228858 4377 bp DNA linear 22-Nov-2024 DEFINITION AND GATE Sal+SupD+LacP+RBS(J61127)+T7ptag ACCESSION BBa_K228858 VERSION BBa_K228858.1 FEATURES Location/Qualifiers CDS 1..903 /label=NahR promoter 904..993 /label=Pr promoter promoter 982..1050 /label=pSal promoter 1..1052 /label=BBa_K228004 misc_feature 1061..1196 /label=BBa_K228001 stem_loop 1216..1259 /label=stem_loop misc_feature 1205..1284 /label=BBa_B0010 terminator 1205..1284 /label=BBa_B0010 stem_loop 1300..1319 /label=T7 TE misc_feature 1326..1326 /label=stop polyA_site 1320..1333 /label=polya misc_feature 1293..1333 /label=BBa_B0012 terminator 1293..1333 /label=BBa_B0012 CDS 1342..1429 /label=end of LacI coding region (inactive) misc_feature 1342..1541 /label=BBa_R0010 misc_feature 1430..1467 /label=CAP binding site promoter 1478..1483 /label=-35 promoter 1502..1507 /label=-10 misc_feature 1507..1541 /label=LacI binding site misc_feature 1514..1514 /label=start promoter 1342..1541 /label=BBa_R0010 RBS 1550..1561 /label=BBa_J61127 CDS 1568..4240 /label=cds CDS 1568..4240 /label=BBa_K228000 stem_loop 4260..4303 /label=stem_loop misc_feature 4249..4328 /label=BBa_B0010 terminator 4249..4328 /label=BBa_B0010 stem_loop 4344..4363 /label=T7 TE misc_feature 4370..4370 /label=stop polyA_site 4364..4377 /label=polya misc_feature 4337..4377 /label=BBa_B0012 terminator 4337..4377 /label=BBa_B0012 ORIGIN 1 tcaatccgta aacaggtcaa acatcagttg ccgcaaccaa atattggcta ggtccttgtg 61 gtacttcgca tgccagaaca tgttgatggc tatttcaggc aagacgactg ggtgcggcaa 121 ggcgcttagg ccgaagggct ccacgcagca gtcggctaaa cgtatcggca cagtggcgag 181 cagatcggtg cgctggagga tgtggccaac ggcggcgaag tgcggcactt ccagacggat 241 gtcgcgccgg atgccgaccc gtgtcatgta cgtgtccacc tcgccgtggc cggtgccagc 301 ggcgatgaca cgcacgtggc cgtaggaaca gaagcgctcc agagtcaggg gttcgcgggt 361 gactggatgg tccttgcgac ataggcacac gtagtgattc tggagcagcc ggcgctgaaa 421 gaagccagtt tgcagattgg gaagcaggcc cacggccaag tccacggttc cgttctgcaa 481 ggcctgcatc aggctcatcg aactgtcgcg caccgtactg atcacgcaat tgggggcctg 541 gtgagccagc acatccatca gccgcggcat gaagtagatc tcgccaatgt cggtcatggc 601 cagggtgaag gtacgctcgc tggtcagcgg atcgaagctt tcatggtgct gtagggcgtt 661 gcgcagtgcg tgcatggccg aagtgacggg ctcggccaga tgcgcggcat agggtgtggg 721 ttccattccc tgatgtgtgc gcacgaagag tgggtcctgt agcgaggtgc gcaggcgttt 781 cagcgcattg ctcacggcag gctgggtcag gcccaggttc tccgcagtga tagagacgcg 841 tctgtcgacc agcaactggt tgaacaccac cagcaggttt aaatccaggt cacgcagttc 901 catggggcct cgcttgggtt attgctggtg cccggccggg cgcaatattc atgttgatga 961 tttattatat atcgagtggt gtatttatca atattgtttg ctccgttatc gttattaaca 1021 agtcatcaat aaagccatca cgagtaccat agtactagag caattcggag agatgccgga 1081 gcggctgaac ggaccggtct ctaaaaccgg agtaggggca actctaccgg gggttcaaat 1141 ccccctctct ccgccactac agatccttag cgaaagctaa ggattttttt taagcttact 1201 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 1261 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct 1321 ttctgcgttt atatactaga gcaatacgca aaccgcctct ccccgcgcgt tggccgattc 1381 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa 1441 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc 1501 gtatgttgtg tggaattgtg agcggataac aatttcacac atactagaga aagagtggaa 1561 ctactagatg accatgatta ccgtgcacta gaataccatt aacattgcta agaacgactt 1621 ctctgacatc gaactggctg ctatcccgtt caacactctg gctgaccatt acggtgagcg 1681 tttagctcgc gaacagttgg cccttgagca tgagtcttac gagatgggtg aagcacgctt 1741 ccgcaagatg tttgagcgtc aacttaaagc tggtgaggtt gcggataacg ctgccgccaa 1801 gcctctcatc actaccctac tccctaagat gattgcacgc atcaacgact ggtttgagga 1861 agtgaaagct aagcgcggca agcgcccgac agccttccag ttcctgtagg aaatcaagcc 1921 ggaagccgta gcgtacatca ccattaagac cactctggct tgcctaacca gtgctgacaa 1981 tacaaccgtt caggctgtag caagcgcaat cggtcgggcc attgaggacg aggctcgctt 2041 cggtcgtatc cgtgaccttg aagctaagca cttcaagaaa aacgttgagg aacaactcaa 2101 caagcgcgta gggcacgtct acaagaaagc atttatgcaa gttgtcgagg ctgacatgct 2161 ctctaagggt ctactcggtg gcgaggcgtg gtcttcgtgg cataaggaag actctattca 2221 tgtaggagta cgctgcatcg agatgctcat tgagtcaacc ggaatggtta gcttacaccg 2281 ccaaaatgct ggcgtagtag gtcaagactc tgagactatc gaactcgcac ctgaatacgc 2341 tgaggctatc gcaacccgtg caggtgcgct ggctggcatc tctccgatgt tccaaccttg 2401 cgtagttcct cctaagccgt ggactggcat tactggtggt ggctattggg ctaacggtcg 2461 tcgtcctctg gcgctggtgc gtactcacag taagaaagca ctgatgcgct acgaagacgt 2521 ttacatgcct gaggtgtaca aagcgattaa cattgcgcaa aacaccgcat ggaaaatcaa 2581 caagaaagtc ctagcggtcg ccaacgtaat caccaagtgg aagcattgtc cggtcgagga 2641 catccctgcg attgagcgtg aagaactccc gatgaaaccg gaagacatcg acatgaatcc 2701 tgaggctctc accgcgtgga aacgtgctgc cgctgctgtg taccgcaagg acaaggctcg 2761 caagtctcgc cgtatcagcc ttgagttcat gcttgagcaa gccaataagt ttgctaacca 2821 taaggccatc tggttccctt acaacatgga ctggcgcggt cgtgtttacg ctgtgtcaat 2881 gttcaacccg caaggtaacg atatgaccaa aggactgctt acgctggcga aaggtaaacc 2941 aatcggtaag gaaggttact actggctgaa aatccacggt gcaaactgtg cgggtgtcga 3001 taaggttccg ttccctgagc gcatcaagtt cattgaggaa aaccacgaga acatcatggc 3061 ttgcgctaag tctccactgg agaacacttg gtgggctgag caagattctc cgttctgctt 3121 ccttgcgttc tgctttgagt acgctggggt acagcaccac ggcctgagct ataactgctc 3181 ccttccgctg gcgtttgacg ggtcttgctc tggcatccag cacttctccg cgatgctccg 3241 agatgaggta ggtggtcgcg cggttaactt gcttcctagt gaaaccgttc aggacatcta 3301 cgggattgtt gctaagaaag tcaacgagat tctacaagca gacgcaatca atgggaccga 3361 taacgaagta gttaccgtga ccgatgagaa cactggtgaa atctctgaga aagtcaagct 3421 gggcactaag gcactggctg gtcaatggct ggcttacggt gttactcgca gtgtgactaa 3481 gcgttcagtc atgacgctgg cttacgggtc caaagagttc ggcttccgtc aacaagtgct 3541 ggaagatacc attcagccag ctattgattc cggcaagggt ctgatgttca ctcagccgaa 3601 tcaggctgct ggatacatgg ctaagctgat ttgggaatct gtgagcgtga cggtggtagc 3661 tgcggttgaa gcaatgaact ggcttaagtc tgctgctaag ctgctggctg ctgaggtcaa 3721 agataagaag actggagaga ttcttcgcaa gcgttgcgct gtgcattggg taactcctga 3781 tggtttccct gtgtggcagg aatacaagaa gcctattcag acgcgcttga acctgatgtt 3841 cctcggtcag ttccgcttac agcctaccat taacaccaac aaagatagcg agattgatgc 3901 acacaaacag gagtctggta tcgctcctaa ctttgtacac agccaagacg gtagccacct 3961 tcgtaagact gtagtgtggg cacacgagaa gtacggaatc gaatcttttg cactgattca 4021 cgactccttc ggtaccattc cggctgacgc tgcgaacctg ttcaaagcag tgcgcgaaac 4081 tatggttgac acatatgagt cttgtgatgt actggctgat ttctacgacc agttcgctga 4141 ccagttgcac gagtctcaat tggacaaaat gccagcactt ccggctaaag gtaacttgaa 4201 cctccgtgac atcttagagt cggacttcgc gttcgcataa tactagagcc aggcatcaaa 4261 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga 4321 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata //