##gff-version 3
##sequence-region BBa_K228859 1 4376
BBa_K228859	.	terminator	1293	1333	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K228859	.	stem_loop	1300	1319	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K228859	.	polyA_site	1320	1333	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K228859	.	engineered_region	1293	1333	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K228859	.	stop_codon	1326	1326	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K228859	.	promoter	1342	1541	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_K228859	.	non_covalent_binding_site	1430	1467	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K228859	.	promoter	1502	1507	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K228859	.	non_covalent_binding_site	1507	1541	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K228859	.	engineered_region	1342	1541	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K228859	.	promoter	1478	1483	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K228859	.	start_codon	1514	1514	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K228859	.	CDS	1342	1429	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K228859	.	promoter	1	1052	.	+	0	ID=BBa_K228004;Name=BBa_K228004
BBa_K228859	.	promoter	904	993	.	+	0	ID=annotation2075286;Name=Pr promoter;Parent=BBa_K228004
BBa_K228859	.	promoter	982	1050	.	+	0	ID=annotation2075287;Name=pSal;Parent=BBa_K228004
BBa_K228859	.	CDS	1	903	.	+	0	ID=annotation2075285;Name=NahR;Parent=BBa_K228004
BBa_K228859	.	mature_transcript_region	1061	1196	.	+	0	ID=BBa_K228001;Name=BBa_K228001
BBa_K228859	.	ribosome_entry_site	1550	1560	.	+	0	ID=BBa_B0033;Name=BBa_B0033
BBa_K228859	.	ribosome_entry_site	1550	1560	.	+	0	ID=annotation1713;Name=RBS-4\Weaker;Parent=BBa_B0033
BBa_K228859	.	engineered_region	1550	1560	.	+	0	ID=annotation7028;Name=BBa_B0033;Parent=BBa_B0033
BBa_K228859	.	ribosome_entry_site	1556	1559	.	+	0	ID=annotation1714;Name=RBS;Parent=BBa_B0033
BBa_K228859	.	terminator	4336	4376	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K228859	.	stem_loop	4343	4362	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K228859	.	polyA_site	4363	4376	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K228859	.	engineered_region	4336	4376	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K228859	.	stop_codon	4369	4369	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K228859	.	CDS	1567	4239	.	+	0	ID=BBa_K228000;Name=BBa_K228000
BBa_K228859	.	CDS	1567	4239	.	+	0	ID=annotation2017714;Name=cds;Parent=BBa_K228000
BBa_K228859	.	terminator	4248	4327	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K228859	.	engineered_region	4248	4327	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K228859	.	stem_loop	4259	4302	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K228859	.	terminator	1205	1284	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K228859	.	engineered_region	1205	1284	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K228859	.	stem_loop	1216	1259	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
>BBa_K228859
tcaatccgtaaacaggtcaaacatcagttgccgcaaccaaatattggctaggtccttgtggtacttcgcatgccagaac
atgttgatggctatttcaggcaagacgactgggtgcggcaaggcgcttaggccgaagggctccacgcagcagtcggcta
aacgtatcggcacagtggcgagcagatcggtgcgctggaggatgtggccaacggcggcgaagtgcggcacttccagacg
gatgtcgcgccggatgccgacccgtgtcatgtacgtgtccacctcgccgtggccggtgccagcggcgatgacacgcacg
tggccgtaggaacagaagcgctccagagtcaggggttcgcgggtgactggatggtccttgcgacataggcacacgtagt
gattctggagcagccggcgctgaaagaagccagtttgcagattgggaagcaggcccacggccaagtccacggttccgtt
ctgcaaggcctgcatcaggctcatcgaactgtcgcgcaccgtactgatcacgcaattgggggcctggtgagccagcaca
tccatcagccgcggcatgaagtagatctcgccaatgtcggtcatggccagggtgaaggtacgctcgctggtcagcggat
cgaagctttcatggtgctgtagggcgttgcgcagtgcgtgcatggccgaagtgacgggctcggccagatgcgcggcata
gggtgtgggttccattccctgatgtgtgcgcacgaagagtgggtcctgtagcgaggtgcgcaggcgtttcagcgcattg
ctcacggcaggctgggtcaggcccaggttctccgcagtgatagagacgcgtctgtcgaccagcaactggttgaacacca
ccagcaggtttaaatccaggtcacgcagttccatggggcctcgcttgggttattgctggtgcccggccgggcgcaatat
tcatgttgatgatttattatatatcgagtggtgtatttatcaatattgtttgctccgttatcgttattaacaagtcatc
aataaagccatcacgagtaccatagtactagagcaattcggagagatgccggagcggctgaacggaccggtctctaaaa
ccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactacagatccttagcgaaagctaaggatt
ttttttaagcttactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgtt
gtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagca
atacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgg
gcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcg
tatgttgtgtggaattgtgagcggataacaatttcacacatactagagtcacacaggactactagatgaccatgattac
cgtgcactagaataccattaacattgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctg
gctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgct
tccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccct
actccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttc
cagttcctgtaggaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctg
acaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtga
ccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcattt
atgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagact
ctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctgg
cgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctg
gctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattggg
ctaacggtcgtcgtcctctggcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatgcc
tgaggtgtacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgta
atcaccaagtggaagcattgtccggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaagaca
tcgacatgaatcctgaggctctcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtc
tcgccgtatcagccttgagttcatgcttgagcaagccaataagtttgctaaccataaggccatctggttcccttacaac
atggactggcgcggtcgtgtttacgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgc
tggcgaaaggtaaaccaatcggtaaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgataa
ggttccgttccctgagcgcatcaagttcattgaggaaaaccacgagaacatcatggcttgcgctaagtctccactggag
aacacttggtgggctgagcaagattctccgttctgcttccttgcgttctgctttgagtacgctggggtacagcaccacg
gcctgagctataactgctcccttccgctggcgtttgacgggtcttgctctggcatccagcacttctccgcgatgctccg
agatgaggtaggtggtcgcgcggttaacttgcttcctagtgaaaccgttcaggacatctacgggattgttgctaagaaa
gtcaacgagattctacaagcagacgcaatcaatgggaccgataacgaagtagttaccgtgaccgatgagaacactggtg
aaatctctgagaaagtcaagctgggcactaaggcactggctggtcaatggctggcttacggtgttactcgcagtgtgac
taagcgttcagtcatgacgctggcttacgggtccaaagagttcggcttccgtcaacaagtgctggaagataccattcag
ccagctattgattccggcaagggtctgatgttcactcagccgaatcaggctgctggatacatggctaagctgatttggg
aatctgtgagcgtgacggtggtagctgcggttgaagcaatgaactggcttaagtctgctgctaagctgctggctgctga
ggtcaaagataagaagactggagagattcttcgcaagcgttgcgctgtgcattgggtaactcctgatggtttccctgtg
tggcaggaatacaagaagcctattcagacgcgcttgaacctgatgttcctcggtcagttccgcttacagcctaccatta
acaccaacaaagatagcgagattgatgcacacaaacaggagtctggtatcgctcctaactttgtacacagccaagacgg
tagccaccttcgtaagactgtagtgtgggcacacgagaagtacggaatcgaatcttttgcactgattcacgactccttc
ggtaccattccggctgacgctgcgaacctgttcaaagcagtgcgcgaaactatggttgacacatatgagtcttgtgatg
tactggctgatttctacgaccagttcgctgaccagttgcacgagtctcaattggacaaaatgccagcacttccggctaa
aggtaacttgaacctccgtgacatcttagagtcggacttcgcgttcgcataatactagagccaggcatcaaataaaacg
aaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggc
tcaccttcgggtgggcctttctgcgtttata