LOCUS BBa_K228860 4379 bp DNA linear 11-Dec-2025 DEFINITION AND GATE Sal+SupD+LacP+RBS(B0031)+T7ptag ACCESSION BBa_K228860 VERSION BBa_K228860.1 FEATURES Location/Qualifiers CDS 1..903 /label=NahR promoter 904..993 /label=Pr promoter promoter 982..1050 /label=pSal promoter 1..1052 /label=BBa_K228004 misc_feature 1061..1196 /label=BBa_K228001 stem_loop 1216..1259 /label=stem_loop misc_feature 1205..1284 /label=BBa_B0010 terminator 1205..1284 /label=BBa_B0010 stem_loop 1300..1319 /label=T7 TE misc_feature 1326..1326 /label=stop polyA_site 1320..1333 /label=polya misc_feature 1293..1333 /label=BBa_B0012 terminator 1293..1333 /label=BBa_B0012 CDS 1342..1429 /label=end of LacI coding region (inactive) misc_feature 1342..1541 /label=BBa_R0010 misc_feature 1430..1467 /label=CAP binding site promoter 1478..1483 /label=-35 promoter 1502..1507 /label=-10 misc_feature 1507..1541 /label=LacI binding site misc_feature 1514..1514 /label=start promoter 1342..1541 /label=BBa_R0010 misc_feature 1556..1559 /label=conserved RBS 1550..1563 /label=BBa_B0031 CDS 1570..4242 /label=cds CDS 1570..4242 /label=BBa_K228000 stem_loop 4262..4305 /label=stem_loop misc_feature 4251..4330 /label=BBa_B0010 terminator 4251..4330 /label=BBa_B0010 stem_loop 4346..4365 /label=T7 TE misc_feature 4372..4372 /label=stop polyA_site 4366..4379 /label=polya misc_feature 4339..4379 /label=BBa_B0012 terminator 4339..4379 /label=BBa_B0012 ORIGIN 1 tcaatccgta aacaggtcaa acatcagttg ccgcaaccaa atattggcta ggtccttgtg 61 gtacttcgca tgccagaaca tgttgatggc tatttcaggc aagacgactg ggtgcggcaa 121 ggcgcttagg ccgaagggct ccacgcagca gtcggctaaa cgtatcggca cagtggcgag 181 cagatcggtg cgctggagga tgtggccaac ggcggcgaag tgcggcactt ccagacggat 241 gtcgcgccgg atgccgaccc gtgtcatgta cgtgtccacc tcgccgtggc cggtgccagc 301 ggcgatgaca cgcacgtggc cgtaggaaca gaagcgctcc agagtcaggg gttcgcgggt 361 gactggatgg tccttgcgac ataggcacac gtagtgattc tggagcagcc ggcgctgaaa 421 gaagccagtt tgcagattgg gaagcaggcc cacggccaag tccacggttc cgttctgcaa 481 ggcctgcatc aggctcatcg aactgtcgcg caccgtactg atcacgcaat tgggggcctg 541 gtgagccagc acatccatca gccgcggcat gaagtagatc tcgccaatgt cggtcatggc 601 cagggtgaag gtacgctcgc tggtcagcgg atcgaagctt tcatggtgct gtagggcgtt 661 gcgcagtgcg tgcatggccg aagtgacggg ctcggccaga tgcgcggcat agggtgtggg 721 ttccattccc tgatgtgtgc gcacgaagag tgggtcctgt agcgaggtgc gcaggcgttt 781 cagcgcattg ctcacggcag gctgggtcag gcccaggttc tccgcagtga tagagacgcg 841 tctgtcgacc agcaactggt tgaacaccac cagcaggttt aaatccaggt cacgcagttc 901 catggggcct cgcttgggtt attgctggtg cccggccggg cgcaatattc atgttgatga 961 tttattatat atcgagtggt gtatttatca atattgtttg ctccgttatc gttattaaca 1021 agtcatcaat aaagccatca cgagtaccat agtactagag caattcggag agatgccgga 1081 gcggctgaac ggaccggtct ctaaaaccgg agtaggggca actctaccgg gggttcaaat 1141 ccccctctct ccgccactac agatccttag cgaaagctaa ggattttttt taagcttact 1201 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 1261 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct 1321 ttctgcgttt atatactaga gcaatacgca aaccgcctct ccccgcgcgt tggccgattc 1381 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa 1441 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc 1501 gtatgttgtg tggaattgtg agcggataac aatttcacac atactagagt cacacaggaa 1561 acctactaga tgaccatgat taccgtgcac tagaatacca ttaacattgc taagaacgac 1621 ttctctgaca tcgaactggc tgctatcccg ttcaacactc tggctgacca ttacggtgag 1681 cgtttagctc gcgaacagtt ggcccttgag catgagtctt acgagatggg tgaagcacgc 1741 ttccgcaaga tgtttgagcg tcaacttaaa gctggtgagg ttgcggataa cgctgccgcc 1801 aagcctctca tcactaccct actccctaag atgattgcac gcatcaacga ctggtttgag 1861 gaagtgaaag ctaagcgcgg caagcgcccg acagccttcc agttcctgta ggaaatcaag 1921 ccggaagccg tagcgtacat caccattaag accactctgg cttgcctaac cagtgctgac 1981 aatacaaccg ttcaggctgt agcaagcgca atcggtcggg ccattgagga cgaggctcgc 2041 ttcggtcgta tccgtgacct tgaagctaag cacttcaaga aaaacgttga ggaacaactc 2101 aacaagcgcg tagggcacgt ctacaagaaa gcatttatgc aagttgtcga ggctgacatg 2161 ctctctaagg gtctactcgg tggcgaggcg tggtcttcgt ggcataagga agactctatt 2221 catgtaggag tacgctgcat cgagatgctc attgagtcaa ccggaatggt tagcttacac 2281 cgccaaaatg ctggcgtagt aggtcaagac tctgagacta tcgaactcgc acctgaatac 2341 gctgaggcta tcgcaacccg tgcaggtgcg ctggctggca tctctccgat gttccaacct 2401 tgcgtagttc ctcctaagcc gtggactggc attactggtg gtggctattg ggctaacggt 2461 cgtcgtcctc tggcgctggt gcgtactcac agtaagaaag cactgatgcg ctacgaagac 2521 gtttacatgc ctgaggtgta caaagcgatt aacattgcgc aaaacaccgc atggaaaatc 2581 aacaagaaag tcctagcggt cgccaacgta atcaccaagt ggaagcattg tccggtcgag 2641 gacatccctg cgattgagcg tgaagaactc ccgatgaaac cggaagacat cgacatgaat 2701 cctgaggctc tcaccgcgtg gaaacgtgct gccgctgctg tgtaccgcaa ggacaaggct 2761 cgcaagtctc gccgtatcag ccttgagttc atgcttgagc aagccaataa gtttgctaac 2821 cataaggcca tctggttccc ttacaacatg gactggcgcg gtcgtgttta cgctgtgtca 2881 atgttcaacc cgcaaggtaa cgatatgacc aaaggactgc ttacgctggc gaaaggtaaa 2941 ccaatcggta aggaaggtta ctactggctg aaaatccacg gtgcaaactg tgcgggtgtc 3001 gataaggttc cgttccctga gcgcatcaag ttcattgagg aaaaccacga gaacatcatg 3061 gcttgcgcta agtctccact ggagaacact tggtgggctg agcaagattc tccgttctgc 3121 ttccttgcgt tctgctttga gtacgctggg gtacagcacc acggcctgag ctataactgc 3181 tcccttccgc tggcgtttga cgggtcttgc tctggcatcc agcacttctc cgcgatgctc 3241 cgagatgagg taggtggtcg cgcggttaac ttgcttccta gtgaaaccgt tcaggacatc 3301 tacgggattg ttgctaagaa agtcaacgag attctacaag cagacgcaat caatgggacc 3361 gataacgaag tagttaccgt gaccgatgag aacactggtg aaatctctga gaaagtcaag 3421 ctgggcacta aggcactggc tggtcaatgg ctggcttacg gtgttactcg cagtgtgact 3481 aagcgttcag tcatgacgct ggcttacggg tccaaagagt tcggcttccg tcaacaagtg 3541 ctggaagata ccattcagcc agctattgat tccggcaagg gtctgatgtt cactcagccg 3601 aatcaggctg ctggatacat ggctaagctg atttgggaat ctgtgagcgt gacggtggta 3661 gctgcggttg aagcaatgaa ctggcttaag tctgctgcta agctgctggc tgctgaggtc 3721 aaagataaga agactggaga gattcttcgc aagcgttgcg ctgtgcattg ggtaactcct 3781 gatggtttcc ctgtgtggca ggaatacaag aagcctattc agacgcgctt gaacctgatg 3841 ttcctcggtc agttccgctt acagcctacc attaacacca acaaagatag cgagattgat 3901 gcacacaaac aggagtctgg tatcgctcct aactttgtac acagccaaga cggtagccac 3961 cttcgtaaga ctgtagtgtg ggcacacgag aagtacggaa tcgaatcttt tgcactgatt 4021 cacgactcct tcggtaccat tccggctgac gctgcgaacc tgttcaaagc agtgcgcgaa 4081 actatggttg acacatatga gtcttgtgat gtactggctg atttctacga ccagttcgct 4141 gaccagttgc acgagtctca attggacaaa atgccagcac ttccggctaa aggtaacttg 4201 aacctccgtg acatcttaga gtcggacttc gcgttcgcat aatactagag ccaggcatca 4261 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt 4321 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttata //