##gff-version 3
##sequence-region BBa_K228860 1 4379
BBa_K228860	.	promoter	1342	1541	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_K228860	.	non_covalent_binding_site	1507	1541	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K228860	.	non_covalent_binding_site	1430	1467	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K228860	.	promoter	1478	1483	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K228860	.	start_codon	1514	1514	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K228860	.	engineered_region	1342	1541	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K228860	.	CDS	1342	1429	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K228860	.	promoter	1502	1507	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K228860	.	terminator	1293	1333	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K228860	.	polyA_site	1320	1333	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K228860	.	stop_codon	1326	1326	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K228860	.	stem_loop	1300	1319	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K228860	.	engineered_region	1293	1333	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K228860	.	ribosome_entry_site	1550	1563	.	+	0	ID=BBa_B0031;Name=BBa_B0031
BBa_K228860	.	conserved_region	1556	1559	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_K228860	.	CDS	1570	4242	.	+	0	ID=BBa_K228000;Name=BBa_K228000
BBa_K228860	.	CDS	1570	4242	.	+	0	ID=annotation2017714;Name=cds;Parent=BBa_K228000
BBa_K228860	.	terminator	1205	1284	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K228860	.	stem_loop	1216	1259	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K228860	.	engineered_region	1205	1284	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K228860	.	terminator	4339	4379	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K228860	.	polyA_site	4366	4379	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K228860	.	stop_codon	4372	4372	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K228860	.	stem_loop	4346	4365	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K228860	.	engineered_region	4339	4379	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K228860	.	mature_transcript_region	1061	1196	.	+	0	ID=BBa_K228001;Name=BBa_K228001
BBa_K228860	.	promoter	1	1052	.	+	0	ID=BBa_K228004;Name=BBa_K228004
BBa_K228860	.	promoter	982	1050	.	+	0	ID=annotation2075287;Name=pSal;Parent=BBa_K228004
BBa_K228860	.	promoter	904	993	.	+	0	ID=annotation2075286;Name=Pr promoter;Parent=BBa_K228004
BBa_K228860	.	CDS	1	903	.	+	0	ID=annotation2075285;Name=NahR;Parent=BBa_K228004
BBa_K228860	.	terminator	4251	4330	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K228860	.	stem_loop	4262	4305	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K228860	.	engineered_region	4251	4330	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_K228860
tcaatccgtaaacaggtcaaacatcagttgccgcaaccaaatattggctaggtccttgtggtacttcgcatgccagaac
atgttgatggctatttcaggcaagacgactgggtgcggcaaggcgcttaggccgaagggctccacgcagcagtcggcta
aacgtatcggcacagtggcgagcagatcggtgcgctggaggatgtggccaacggcggcgaagtgcggcacttccagacg
gatgtcgcgccggatgccgacccgtgtcatgtacgtgtccacctcgccgtggccggtgccagcggcgatgacacgcacg
tggccgtaggaacagaagcgctccagagtcaggggttcgcgggtgactggatggtccttgcgacataggcacacgtagt
gattctggagcagccggcgctgaaagaagccagtttgcagattgggaagcaggcccacggccaagtccacggttccgtt
ctgcaaggcctgcatcaggctcatcgaactgtcgcgcaccgtactgatcacgcaattgggggcctggtgagccagcaca
tccatcagccgcggcatgaagtagatctcgccaatgtcggtcatggccagggtgaaggtacgctcgctggtcagcggat
cgaagctttcatggtgctgtagggcgttgcgcagtgcgtgcatggccgaagtgacgggctcggccagatgcgcggcata
gggtgtgggttccattccctgatgtgtgcgcacgaagagtgggtcctgtagcgaggtgcgcaggcgtttcagcgcattg
ctcacggcaggctgggtcaggcccaggttctccgcagtgatagagacgcgtctgtcgaccagcaactggttgaacacca
ccagcaggtttaaatccaggtcacgcagttccatggggcctcgcttgggttattgctggtgcccggccgggcgcaatat
tcatgttgatgatttattatatatcgagtggtgtatttatcaatattgtttgctccgttatcgttattaacaagtcatc
aataaagccatcacgagtaccatagtactagagcaattcggagagatgccggagcggctgaacggaccggtctctaaaa
ccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactacagatccttagcgaaagctaaggatt
ttttttaagcttactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgtt
gtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagca
atacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgg
gcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcg
tatgttgtgtggaattgtgagcggataacaatttcacacatactagagtcacacaggaaacctactagatgaccatgat
taccgtgcactagaataccattaacattgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacact
ctggctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcac
gcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactac
cctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagcc
ttccagttcctgtaggaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtg
ctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccg
tgaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagca
tttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaag
actctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgc
tggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcg
ctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctatt
gggctaacggtcgtcgtcctctggcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacat
gcctgaggtgtacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaac
gtaatcaccaagtggaagcattgtccggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaag
acatcgacatgaatcctgaggctctcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaa
gtctcgccgtatcagccttgagttcatgcttgagcaagccaataagtttgctaaccataaggccatctggttcccttac
aacatggactggcgcggtcgtgtttacgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgctta
cgctggcgaaaggtaaaccaatcggtaaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcga
taaggttccgttccctgagcgcatcaagttcattgaggaaaaccacgagaacatcatggcttgcgctaagtctccactg
gagaacacttggtgggctgagcaagattctccgttctgcttccttgcgttctgctttgagtacgctggggtacagcacc
acggcctgagctataactgctcccttccgctggcgtttgacgggtcttgctctggcatccagcacttctccgcgatgct
ccgagatgaggtaggtggtcgcgcggttaacttgcttcctagtgaaaccgttcaggacatctacgggattgttgctaag
aaagtcaacgagattctacaagcagacgcaatcaatgggaccgataacgaagtagttaccgtgaccgatgagaacactg
gtgaaatctctgagaaagtcaagctgggcactaaggcactggctggtcaatggctggcttacggtgttactcgcagtgt
gactaagcgttcagtcatgacgctggcttacgggtccaaagagttcggcttccgtcaacaagtgctggaagataccatt
cagccagctattgattccggcaagggtctgatgttcactcagccgaatcaggctgctggatacatggctaagctgattt
gggaatctgtgagcgtgacggtggtagctgcggttgaagcaatgaactggcttaagtctgctgctaagctgctggctgc
tgaggtcaaagataagaagactggagagattcttcgcaagcgttgcgctgtgcattgggtaactcctgatggtttccct
gtgtggcaggaatacaagaagcctattcagacgcgcttgaacctgatgttcctcggtcagttccgcttacagcctacca
ttaacaccaacaaagatagcgagattgatgcacacaaacaggagtctggtatcgctcctaactttgtacacagccaaga
cggtagccaccttcgtaagactgtagtgtgggcacacgagaagtacggaatcgaatcttttgcactgattcacgactcc
ttcggtaccattccggctgacgctgcgaacctgttcaaagcagtgcgcgaaactatggttgacacatatgagtcttgtg
atgtactggctgatttctacgaccagttcgctgaccagttgcacgagtctcaattggacaaaatgccagcacttccggc
taaaggtaacttgaacctccgtgacatcttagagtcggacttcgcgttcgcataatactagagccaggcatcaaataaa
acgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacact
ggctcaccttcgggtgggcctttctgcgtttata