BBa_K228863
1
BBa_K228863
PhiR73 delta with amber mutation
2009-09-21T11:00:00Z
2015-05-08T01:11:34Z
part registery 2009 distribution & PKU 2009 igem team
T7 polymerase with an amber mutation coding gene is constructed after AraC protein(reversed sequence) and Pbad promoter which would be triggered by Arabinose. T7 polymerase can work well in the exsitence of SupD tRNA. Besides, the expression of the T7 polymerase can be regulated by RBS.
false
false
_353_
0
4411
9
Not in stock
false
when cut this part for insert and ligate it into a vetor, the insert and vector may have the similar sizes which may disturb the ligation.
false
Lin Min
annotation2044025
1
cds
range2044025
1
1
243
annotation2044056
1
TAG amber mutation
range2044056
1
25
27
BBa_K228863_sequence
1
atgcgctgccctttctgtcgtcattaggcgcatacccgcaccagccggtatgtgagtgacaatgtcaaagaaagttatctccagtgccagaatatttactgttcggcgacatttaaaacgcatgagtcaatttgtgccgtgattcgttctccggtcacggaggaaaaaccagcaccggcaagcacagcaccggctgttgtccgaaaagttaaaggctgttacagctcaccattcaaccattaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z