LOCUS       BBa_K228865             1321 bp    DNA     linear       01-Jun-2024
DEFINITION  pSal-rbs-PhiR73 ( amber mutation )
ACCESSION   BBa_K228865
VERSION     BBa_K228865.1  
FEATURES             Location/Qualifiers
     CDS             1..903
                     /label=NahR
     promoter        904..993
                     /label=Pr promoter
     promoter        982..1050
                     /label=pSal
     promoter        1..1052
                     /label=BBa_K228004
     misc_feature    1065..1068
                     /label=conserved
     RBS             1061..1072
                     /label=BBa_B0034
     CDS             1079..1321
                     /label=cds
     misc_feature    1103..1105
                     /label=TAG amber mutation
     CDS             1079..1321
                     /label=BBa_K228863
ORIGIN
        1 tcaatccgta aacaggtcaa acatcagttg ccgcaaccaa atattggcta ggtccttgtg
       61 gtacttcgca tgccagaaca tgttgatggc tatttcaggc aagacgactg ggtgcggcaa
      121 ggcgcttagg ccgaagggct ccacgcagca gtcggctaaa cgtatcggca cagtggcgag
      181 cagatcggtg cgctggagga tgtggccaac ggcggcgaag tgcggcactt ccagacggat
      241 gtcgcgccgg atgccgaccc gtgtcatgta cgtgtccacc tcgccgtggc cggtgccagc
      301 ggcgatgaca cgcacgtggc cgtaggaaca gaagcgctcc agagtcaggg gttcgcgggt
      361 gactggatgg tccttgcgac ataggcacac gtagtgattc tggagcagcc ggcgctgaaa
      421 gaagccagtt tgcagattgg gaagcaggcc cacggccaag tccacggttc cgttctgcaa
      481 ggcctgcatc aggctcatcg aactgtcgcg caccgtactg atcacgcaat tgggggcctg
      541 gtgagccagc acatccatca gccgcggcat gaagtagatc tcgccaatgt cggtcatggc
      601 cagggtgaag gtacgctcgc tggtcagcgg atcgaagctt tcatggtgct gtagggcgtt
      661 gcgcagtgcg tgcatggccg aagtgacggg ctcggccaga tgcgcggcat agggtgtggg
      721 ttccattccc tgatgtgtgc gcacgaagag tgggtcctgt agcgaggtgc gcaggcgttt
      781 cagcgcattg ctcacggcag gctgggtcag gcccaggttc tccgcagtga tagagacgcg
      841 tctgtcgacc agcaactggt tgaacaccac cagcaggttt aaatccaggt cacgcagttc
      901 catggggcct cgcttgggtt attgctggtg cccggccggg cgcaatattc atgttgatga
      961 tttattatat atcgagtggt gtatttatca atattgtttg ctccgttatc gttattaaca
     1021 agtcatcaat aaagccatca cgagtaccat agtactagag aaagaggaga aatactagat
     1081 gcgctgccct ttctgtcgtc attaggcgca tacccgcacc agccggtatg tgagtgacaa
     1141 tgtcaaagaa agttatctcc agtgccagaa tatttactgt tcggcgacat ttaaaacgca
     1201 tgagtcaatt tgtgccgtga ttcgttctcc ggtcacggag gaaaaaccag caccggcaag
     1261 cacagcaccg gctgttgtcc gaaaagttaa aggctgttac agctcaccat tcaaccatta
     1321 a
//