LOCUS       BBa_K228866             2752 bp    DNA     linear       25-Jan-2026
DEFINITION  AND Gate pBad-SupD pSal-PhiR73 delta(amber mutation)
ACCESSION   BBa_K228866
VERSION     BBa_K228866.1  
FEATURES             Location/Qualifiers
     misc_feature    51..54
                     /label=conserved
     RBS             47..58
                     /label=BBa_B0034
     promoter        1150..1279
                     /label=BBa_I13453
     misc_feature    1..38
                     /label=P(Cat)
     promoter        4..9
                     /label=-35
     promoter        27..32
                     /label=-10
     misc_feature    1..38
                     /label=BBa_I14033
     misc_feature    47..58
                     /label=BBa_B0034
     misc_feature    51..54
                     /label=conserved
     misc_feature    65..979
                     /label=BBa_C0080
     CDS             65..940
                     /label=araC
     misc_feature    941..973
                     /label=LVA
     misc_feature    980..1004
                     /label=barcode
     misc_feature    1013..1092
                     /label=BBa_B0010
     stem_loop       1024..1067
                     /label=stem_loop
     misc_feature    1101..1141
                     /label=BBa_B0012
     stem_loop       1108..1127
                     /label=T7 TE
     polyA_site      1128..1141
                     /label=polya
     misc_feature    1134..1134
                     /label=stop
     misc_feature    47..1141
                     /label=BBa_K228006
     misc_feature    1150..1279
                     /label=BBa_I13453
     misc_feature    1..1141
                     /label=BBa_K228008
     promoter        1150..1279
                     /label=BBa_I13453
     promoter        1..1279
                     /label=BBa_K228009
     misc_feature    1288..1423
                     /label=BBa_K228001
     misc_feature    1..1423
                     /label=BBa_K228012
     CDS             1432..2334
                     /label=NahR
     promoter        2335..2424
                     /label=Pr promoter
     promoter        2413..2481
                     /label=pSal
     promoter        1432..2483
                     /label=BBa_K228004
     misc_feature    2496..2499
                     /label=conserved
     RBS             2492..2503
                     /label=BBa_B0034
     CDS             2510..2752
                     /label=cds
     misc_feature    2534..2536
                     /label=TAG amber mutation
     CDS             2510..2752
                     /label=BBa_K228863
     misc_feature    1432..2752
                     /label=BBa_K228865
ORIGIN
        1 tataaacgca gaaaggccca cccgaaggtg agccagtgtg actctagtag agagcgttca
       61 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt
      121 gatgcctggc tctagtagtg atctacacta gcactatcag tgttattaag ctactaaagc
      181 gtagttttcg tcgtttgcag ctgacaactt gacggctaca tcattcactt tttcttcaca
      241 accggcacgg aactcgctcg ggctggcccc ggtgcatttt ttaaatactc gcgagaaata
      301 gagttgatcg tcaaaaccaa cattgcgacc gacggtggcg ataggcatcc gggtagtgct
      361 caaaagcagc ttcgcctggc tgatgcgttg gtcctcgcgc cagcttaaga cgctaatccc
      421 taactgctgg cggaaaagat gtgacagacg cgacggcgac aggcaaacat gctgtgcgac
      481 gctggcgata tcaaaattgc tgtctgccag gtgatcgctg atgtactgac aagcctcgcg
      541 tacccgatta tccatcggtg gatggagcga ctcgttaatc gcttccatgc gccgcagtaa
      601 caattgctca agcagattta tcgccagcag ctccgaatag cgcccttccc cttgcccggc
      661 gttaatgatt tgcccaaaca ggtcgctgaa atgcggctgg tgcgcttcat ccgggcgaaa
      721 gaaacccgta ttggcaaata ttgacggcca gttaagccat tcatgccagt aggcgcgcgg
      781 acgaaagtaa acccactggt gataccattc gcgagcctcc ggatgacgac cgtagtgatg
      841 aatctctcct ggcgggaaca gcaaaatatc acccggtcgg cagacaaatt ctcgtccctg
      901 atttttcacc accccctgac cgcgaatggt gagattgaga atataacctt tcattcccag
      961 cggtcggtcg ataaaaaaat cgagataacc attggcctca atcggcgtta aacccgccac
     1021 cagatgggcg ttaaacgagt atcccggcag caggggatca ttttgcgctt cagccatcta
     1081 gtatttctcc tctttctcta gtatgtttca ttatggtgaa agttggaacc tcttacgtgc
     1141 ctactagaga cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat
     1201 ccataagatt agcggatcct acctgacgct ttttatcgca actctctact gtttctccat
     1261 accgtttttt tgggctagct actagagcaa ttcggagaga tgccggagcg gctgaacgga
     1321 ccggtctcta aaaccggagt aggggcaact ctaccggggg ttcaaatccc cctctctccg
     1381 ccactacaga tccttagcga aagctaagga ttttttttaa gcttactaga gtcaatccgt
     1441 aaacaggtca aacatcagtt gccgcaacca aatattggct aggtccttgt ggtacttcgc
     1501 atgccagaac atgttgatgg ctatttcagg caagacgact gggtgcggca aggcgcttag
     1561 gccgaagggc tccacgcagc agtcggctaa acgtatcggc acagtggcga gcagatcggt
     1621 gcgctggagg atgtggccaa cggcggcgaa gtgcggcact tccagacgga tgtcgcgccg
     1681 gatgccgacc cgtgtcatgt acgtgtccac ctcgccgtgg ccggtgccag cggcgatgac
     1741 acgcacgtgg ccgtaggaac agaagcgctc cagagtcagg ggttcgcggg tgactggatg
     1801 gtccttgcga cataggcaca cgtagtgatt ctggagcagc cggcgctgaa agaagccagt
     1861 ttgcagattg ggaagcaggc ccacggccaa gtccacggtt ccgttctgca aggcctgcat
     1921 caggctcatc gaactgtcgc gcaccgtact gatcacgcaa ttgggggcct ggtgagccag
     1981 cacatccatc agccgcggca tgaagtagat ctcgccaatg tcggtcatgg ccagggtgaa
     2041 ggtacgctcg ctggtcagcg gatcgaagct ttcatggtgc tgtagggcgt tgcgcagtgc
     2101 gtgcatggcc gaagtgacgg gctcggccag atgcgcggca tagggtgtgg gttccattcc
     2161 ctgatgtgtg cgcacgaaga gtgggtcctg tagcgaggtg cgcaggcgtt tcagcgcatt
     2221 gctcacggca ggctgggtca ggcccaggtt ctccgcagtg atagagacgc gtctgtcgac
     2281 cagcaactgg ttgaacacca ccagcaggtt taaatccagg tcacgcagtt ccatggggcc
     2341 tcgcttgggt tattgctggt gcccggccgg gcgcaatatt catgttgatg atttattata
     2401 tatcgagtgg tgtatttatc aatattgttt gctccgttat cgttattaac aagtcatcaa
     2461 taaagccatc acgagtacca tagtactaga gaaagaggag aaatactaga tgcgctgccc
     2521 tttctgtcgt cattaggcgc atacccgcac cagccggtat gtgagtgaca atgtcaaaga
     2581 aagttatctc cagtgccaga atatttactg ttcggcgaca tttaaaacgc atgagtcaat
     2641 ttgtgccgtg attcgttctc cggtcacgga ggaaaaacca gcaccggcaa gcacagcacc
     2701 ggctgttgtc cgaaaagtta aaggctgtta cagctcacca ttcaaccatt aa
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