BBa_K228866
type: ComponentDefinition
persistent identity: BBa K228866
display id: BBa_K228866
version: 1
was generated by: iGEM to SBOL conversion
description: AND Gate pBad-SupD pSal-PhiR73 delta(amber mutation)
created: 2009-09-21T11:00:00Z
modified: 2015-05-08T01:11:34Z
mutable provenance: when cut this part for insert and ligate it into a vetor, the insert and vector may have the similar sizes which may disturb the ligation.
owned by: james
owned by: myers
mutable description: T7 polymerase with an amber mutation coding gene is constructed after AraC protein(reversed sequence) and Pbad promoter which would be triggered by Arabinose. T7 polymerase can work well in the exsitence of SupD tRNA. Besides, the expression of the T7 polymerase can be regulated by RBS.
discontinued: false
dominant: false
experience: experience/Works
group u list: _353_
m user id: 0
owner id: 4411
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: part registery 2009 distribution & PKU igem 2009 team
star: false
creator: Lin Min
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K228012
component: BBa_B0034
component: BBa_K228865
sequence annotation: BBa_K228012
sequence annotation: BBa_B0034
sequence annotation: BBa_K228865
sequence: https://synbiohub.org/public/igem/BBa_K228866_sequence/1

BBa_K228012
type: Component
persistent identity: component2262683
display id: component2262683
version: 1
top level: BBa_K228866
definition: BBa_K228012
access: public

BBa_B0034
type: Component
persistent identity: component2262667
display id: component2262667
version: 1
top level: BBa_K228866
definition: BBa_B0034
access: public

BBa_K228865
type: Component
persistent identity: component2262693
display id: component2262693
version: 1
top level: BBa_K228866
definition: BBa_K228865
access: public

BBa_K228012
type: SequenceAnnotation
persistent identity: annotation2262683
display id: annotation2262683
version: 1
top level: BBa_K228866
location: https://synbiohub.org/public/igem/BBa_K228866/annotation2262683/range2262683/1
component: BBa_K228012

https://synbiohub.org/public/igem/BBa_K228866/annotation2262683/range2262683/1
type: Range
persistent identity: range2262683
display id: range2262683
version: 1
top level: BBa_K228866
direction: direction/reverse
start: 1
end: 1423
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2262667
display id: annotation2262667
version: 1
top level: BBa_K228866
location: https://synbiohub.org/public/igem/BBa_K228866/annotation2262667/range2262667/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K228866/annotation2262667/range2262667/1
type: Range
persistent identity: range2262667
display id: range2262667
version: 1
top level: BBa_K228866
direction: direction/reverse
start: 47
end: 58
orientation: inline

BBa_K228865
type: SequenceAnnotation
persistent identity: annotation2262693
display id: annotation2262693
version: 1
top level: BBa_K228866
location: https://synbiohub.org/public/igem/BBa_K228866/annotation2262693/range2262693/1
component: BBa_K228865

https://synbiohub.org/public/igem/BBa_K228866/annotation2262693/range2262693/1
type: Range
persistent identity: range2262693
display id: range2262693
version: 1
top level: BBa_K228866
direction: direction/reverse
start: 1432
end: 2752
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_K228865
type: ComponentDefinition
persistent identity: BBa K228865
display id: BBa_K228865
version: 1
was generated by: iGEM to SBOL conversion
description: pSal-rbs-PhiR73 ( amber mutation )
created: 2009-09-21T11:00:00Z
modified: 2015-05-08T01:11:34Z
mutable provenance: part registery 2009 distribution & PKU 2009 igem team
owned by: james
owned by: myers
mutable description: T7 polymerase with an amber mutation coding gene is constructed after AraC protein(reversed sequence) and Pbad promoter which would be triggered by Arabinose. T7 polymerase can work well in the exsitence of SupD tRNA. Besides, the expression of the T7 polymerase can be regulated by RBS.
discontinued: false
dominant: false
experience: experience/Works
group u list: _353_
m user id: 0
owner id: 4411
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: when cut this part for insert and ligate it into a vetor, the insert and vector may have the similar sizes which may disturb the ligation.
star: false
creator: Lin Min
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K228004
component: BBa_K228863
component: BBa_B0034
sequence annotation: BBa_K228004
sequence annotation: BBa_B0034
sequence annotation: BBa_K228863
sequence: https://synbiohub.org/public/igem/BBa_K228865_sequence/1

BBa_K228004
type: Component
persistent identity: component2044226
display id: component2044226
version: 1
top level: BBa_K228865
definition: BBa_K228004
access: public

BBa_K228863
type: Component
persistent identity: component2044231
display id: component2044231
version: 1
top level: BBa_K228865
definition: BBa_K228863
access: public

BBa_B0034
type: Component
persistent identity: component2044228
display id: component2044228
version: 1
top level: BBa_K228865
definition: BBa_B0034
access: public

BBa_K228004
type: SequenceAnnotation
persistent identity: annotation2044226
display id: annotation2044226
version: 1
top level: BBa_K228865
location: https://synbiohub.org/public/igem/BBa_K228865/annotation2044226/range2044226/1
component: BBa_K228004

https://synbiohub.org/public/igem/BBa_K228865/annotation2044226/range2044226/1
type: Range
persistent identity: range2044226
display id: range2044226
version: 1
top level: BBa_K228865
direction: direction/reverse
start: 1
end: 1052
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2044228
display id: annotation2044228
version: 1
top level: BBa_K228865
location: https://synbiohub.org/public/igem/BBa_K228865/annotation2044228/range2044228/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K228865/annotation2044228/range2044228/1
type: Range
persistent identity: range2044228
display id: range2044228
version: 1
top level: BBa_K228865
direction: direction/reverse
start: 1061
end: 1072
orientation: inline

BBa_K228863
type: SequenceAnnotation
persistent identity: annotation2044231
display id: annotation2044231
version: 1
top level: BBa_K228865
location: https://synbiohub.org/public/igem/BBa_K228865/annotation2044231/range2044231/1
component: BBa_K228863

https://synbiohub.org/public/igem/BBa_K228865/annotation2044231/range2044231/1
type: Range
persistent identity: range2044231
display id: range2044231
version: 1
top level: BBa_K228865
direction: direction/reverse
start: 1079
end: 1321
orientation: inline

BBa_K228012
type: ComponentDefinition
persistent identity: BBa K228012
display id: BBa_K228012
version: 1
was generated by: iGEM to SBOL conversion
description: AraC-Pbad-SupD
created: 2009-09-21T11:00:00Z
modified: 2015-05-08T01:11:32Z
mutable provenance: PKU_Beijing iGEM 2009 team
owned by: james
owned by: myers
mutable description: The transcription of SupD-tRNA is regulated by the Pbad promoter(<partinfo>BBa_I13453</partinfo>). Arabinose can activate the transciprtion by binding to AraC regulatory protein.
discontinued: false
dominant: false
experience: experience/Works
group u list: _353_
m user id: 0
owner id: 4254
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
star: false
creator: Gao Rencheng
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K228001
component: BBa_K228009
sequence annotation: BBa_K228009
sequence annotation: BBa_K228001
sequence: https://synbiohub.org/public/igem/BBa_K228012_sequence/1

BBa_K228001
type: Component
persistent identity: component2246597
display id: component2246597
version: 1
top level: BBa_K228012
definition: BBa_K228001
access: public

BBa_K228009
type: Component
persistent identity: component2246596
display id: component2246596
version: 1
top level: BBa_K228012
definition: BBa_K228009
access: public

BBa_K228009
type: SequenceAnnotation
persistent identity: annotation2246596
display id: annotation2246596
version: 1
top level: BBa_K228012
location: https://synbiohub.org/public/igem/BBa_K228012/annotation2246596/range2246596/1
component: BBa_K228009

https://synbiohub.org/public/igem/BBa_K228012/annotation2246596/range2246596/1
type: Range
persistent identity: range2246596
display id: range2246596
version: 1
top level: BBa_K228012
direction: direction/reverse
start: 1
end: 1279
orientation: inline

BBa_K228001
type: SequenceAnnotation
persistent identity: annotation2246597
display id: annotation2246597
version: 1
top level: BBa_K228012
location: https://synbiohub.org/public/igem/BBa_K228012/annotation2246597/range2246597/1
component: BBa_K228001

https://synbiohub.org/public/igem/BBa_K228012/annotation2246597/range2246597/1
type: Range
persistent identity: range2246597
display id: range2246597
version: 1
top level: BBa_K228012
direction: direction/reverse
start: 1288
end: 1423
orientation: inline

BBa_K228001
type: ComponentDefinition
persistent identity: BBa K228001
display id: BBa_K228001
version: 1
was generated by: iGEM to SBOL conversion
description: SupD-tRNA
created: 2009-08-24T11:00:00Z
modified: 2015-05-08T01:11:32Z
mutable provenance: SupD-tRNA again is from Voigt Lab(UCSF), We standardlize this part by PCR strategy.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The SupD-tRNA part functions as a tRNA, it suppresses the TAG amber stop codon and translate it into a Ser. There is no need to assembly SupD-tRNA with a rbs, since it is not translated. This part, together with the T7ptag part, make an AND Gate, that is, in the presence of both the 2 parts, T7 polymerase is tranlated into a functional polymerase that transcrips a downstream element constructed after a T7 promoter. Which is the output of the AND Gate.
discontinued: false
dominant: false
experience: experience/Works
group u list: _353_
m user id: 0
owner id: 4253
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: There is a PstI site in the original sequence, but luckily it is near the 3' end of this part, so we designed a mutation in our primer and mutated the PstI site.
star: false
creator: Lin Min
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence: https://synbiohub.org/public/igem/BBa_K228001_sequence/1

BBa_K228009
type: ComponentDefinition
persistent identity: BBa K228009
display id: BBa_K228009
version: 1
was generated by: iGEM to SBOL conversion
description: AraC protein(reversed sequence) and Pbad promoter
created: 2009-09-08T11:00:00Z
modified: 2015-05-08T01:11:32Z
owned by: james
owned by: myers
mutable description: This part contains AraC protein generator(BBa_K228008) and Pbad promoter(BBa_I13453). The expression of any coding sequence following the Pbad promoter is repressed constitutively by AraC protein and induced by L-arabinose.
discontinued: false
dominant: false
experience: experience/Works
group u list: _353_
m user id: 0
owner id: 4254
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: The maximum concentration of L-arabinose we tested is 10^-4 M.
star: false
creator: Gao Rencheng
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
component: BBa_K228008
component: BBa_I13453
sequence annotation: BBa_K228008
sequence annotation: BBa_I13453
sequence: https://synbiohub.org/public/igem/BBa_K228009_sequence/1

BBa_K228008
type: Component
persistent identity: component2244233
display id: component2244233
version: 1
top level: BBa_K228009
definition: BBa_K228008
access: public

BBa_I13453
type: Component
persistent identity: component2244232
display id: component2244232
version: 1
top level: BBa_K228009
definition: BBa_I13453
access: public

BBa_I13453
type: Component
persistent identity: component2244234
display id: component2244234
version: 1
top level: BBa_K228009
definition: BBa_I13453
access: public

BBa_K228008
type: SequenceAnnotation
persistent identity: annotation2244233
display id: annotation2244233
version: 1
top level: BBa_K228009
location: https://synbiohub.org/public/igem/BBa_K228009/annotation2244233/range2244233/1
component: BBa_K228008

https://synbiohub.org/public/igem/BBa_K228009/annotation2244233/range2244233/1
type: Range
persistent identity: range2244233
display id: range2244233
version: 1
top level: BBa_K228009
direction: direction/reverse
start: 1
end: 1141
orientation: inline

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation2244232
display id: annotation2244232
version: 1
top level: BBa_K228009
location: https://synbiohub.org/public/igem/BBa_K228009/annotation2244232/range2244232/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_K228009/annotation2244232/range2244232/1
type: Range
persistent identity: range2244232
display id: range2244232
version: 1
top level: BBa_K228009
direction: direction/reverse
start: 1150
end: 1279
orientation: inline

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation2244234
display id: annotation2244234
version: 1
top level: BBa_K228009
location: https://synbiohub.org/public/igem/BBa_K228009/annotation2244234/range2244234/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_K228009/annotation2244234/range2244234/1
type: Range
persistent identity: range2244234
display id: range2244234
version: 1
top level: BBa_K228009
direction: direction/reverse
start: 1150
end: 1279
orientation: inline

BBa_K228004
type: ComponentDefinition
persistent identity: BBa K228004
display id: BBa_K228004
version: 1
was generated by: iGEM to SBOL conversion
description: NahR( reverse) - salicylate promoter
created: 2009-09-17T11:00:00Z
modified: 2015-05-08T01:11:32Z
mutable provenance: It is constrcuted by PCR from the plasmid kindly provided by Voigt Lab in UCSF.
owned by: james
owned by: myers
mutable description: This is a salicylate inducible promoter part. The activator protein is right upstream but in a opposite direction of the regulated promoter. In the presence of salicylate, the promoter can be activated.
discontinued: false
dominant: false
experience: experience/Works
group u list: _353_
m user id: 0
owner id: 4253
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: There is no termoinator downstream of the NahR coding sequence, but it has no effect on parts that are in the same direction as the pSal promoter. However, if coding gene is placed upstream of this part and in the same direction as NahR, it may cause unwanted expression.
star: false
creator: Lin Min
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: NahR
sequence annotation: Pr promoter
sequence annotation: pSal
sequence: https://synbiohub.org/public/igem/BBa_K228004_sequence/1

NahR
type: SequenceAnnotation
persistent identity: annotation2075285
display id: annotation2075285
version: 1
top level: BBa_K228004
location: https://synbiohub.org/public/igem/BBa_K228004/annotation2075285/range2075285/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K228004/annotation2075285/range2075285/1
type: Range
persistent identity: range2075285
display id: range2075285
version: 1
top level: BBa_K228004
direction: direction/reverse
start: 1
end: 903
orientation: inline

Pr promoter
type: SequenceAnnotation
persistent identity: annotation2075286
display id: annotation2075286
version: 1
top level: BBa_K228004
location: https://synbiohub.org/public/igem/BBa_K228004/annotation2075286/range2075286/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K228004/annotation2075286/range2075286/1
type: Range
persistent identity: range2075286
display id: range2075286
version: 1
top level: BBa_K228004
direction: direction/reverse
start: 904
end: 993
orientation: inline

pSal
type: SequenceAnnotation
persistent identity: annotation2075287
display id: annotation2075287
version: 1
top level: BBa_K228004
location: https://synbiohub.org/public/igem/BBa_K228004/annotation2075287/range2075287/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K228004/annotation2075287/range2075287/1
type: Range
persistent identity: range2075287
display id: range2075287
version: 1
top level: BBa_K228004
direction: direction/forward
start: 982
end: 1050
orientation: inline

BBa_K228863
type: ComponentDefinition
persistent identity: BBa K228863
display id: BBa_K228863
version: 1
was generated by: iGEM to SBOL conversion
description: PhiR73 delta with amber mutation
created: 2009-09-21T11:00:00Z
modified: 2015-05-08T01:11:34Z
mutable provenance: part registery 2009 distribution & PKU 2009 igem team
owned by: james
owned by: myers
mutable description: T7 polymerase with an amber mutation coding gene is constructed after AraC protein(reversed sequence) and Pbad promoter which would be triggered by Arabinose. T7 polymerase can work well in the exsitence of SupD tRNA. Besides, the expression of the T7 polymerase can be regulated by RBS.
discontinued: false
dominant: false
experience: experience/Works
group u list: _353_
m user id: 0
owner id: 4411
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: when cut this part for insert and ligate it into a vetor, the insert and vector may have the similar sizes which may disturb the ligation.
star: false
creator: Lin Min
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: cds
sequence annotation: TAG amber mutation
sequence: https://synbiohub.org/public/igem/BBa_K228863_sequence/1

cds
type: SequenceAnnotation
persistent identity: annotation2044025
display id: annotation2044025
version: 1
top level: BBa_K228863
location: https://synbiohub.org/public/igem/BBa_K228863/annotation2044025/range2044025/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K228863/annotation2044025/range2044025/1
type: Range
persistent identity: range2044025
display id: range2044025
version: 1
top level: BBa_K228863
direction: direction/forward
start: 1
end: 243
orientation: inline

TAG amber mutation
type: SequenceAnnotation
persistent identity: annotation2044056
display id: annotation2044056
version: 1
top level: BBa_K228863
location: https://synbiohub.org/public/igem/BBa_K228863/annotation2044056/range2044056/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_K228863/annotation2044056/range2044056/1
type: Range
persistent identity: range2044056
display id: range2044056
version: 1
top level: BBa_K228863
direction: direction/forward
start: 25
end: 27
orientation: inline

BBa_I13453
type: ComponentDefinition
persistent identity: BBa I13453
display id: BBa_I13453
version: 1
was generated by: iGEM to SBOL conversion
description: Pbad promoter
created: 2005-05-24T11:00:00Z
modified: 2015-08-31T04:07:34Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: PBad promoter from I0500 without AraC.
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jkm
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I13453_sequence/1

BBa_K228008
type: ComponentDefinition
persistent identity: BBa K228008
display id: BBa_K228008
version: 1
was generated by: iGEM to SBOL conversion
description: AraC regulatory protein
created: 2009-09-08T11:00:00Z
modified: 2015-05-08T01:11:32Z
owned by: james
owned by: myers
mutable description: This part could be connected to a protein coding sequence so that the expression could be induced by L-arabinose
discontinued: false
dominant: false
experience: experience/Works
group u list: _353_
m user id: 0
owner id: 4254
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
star: false
creator: Gao Rencheng
type: DnaRegion
role: partType/Generator
role: SO:0000804
sequence annotation: BBa_I14033
sequence annotation: P(Cat)
sequence annotation: -35
sequence annotation: -10
sequence annotation: BBa_B0034
sequence annotation: BBa_K228006
sequence annotation: conserved
sequence annotation: araC
sequence annotation: BBa_C0080
sequence annotation: LVA
sequence annotation: barcode
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence annotation: BBa_I13453
sequence: https://synbiohub.org/public/igem/BBa_K228008_sequence/1

BBa_I14033
type: SequenceAnnotation
persistent identity: annotation2020002
display id: annotation2020002
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020002/range2020002/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K228008/annotation2020002/range2020002/1
type: Range
persistent identity: range2020002
display id: range2020002
version: 1
top level: BBa_K228008
direction: direction/reverse
start: 1
end: 38
orientation: inline

P(Cat)
type: SequenceAnnotation
persistent identity: annotation2019999
display id: annotation2019999
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2019999/range2019999/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_K228008/annotation2019999/range2019999/1
type: Range
persistent identity: range2019999
display id: range2019999
version: 1
top level: BBa_K228008
direction: direction/forward
start: 1
end: 38
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2020000
display id: annotation2020000
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020000/range2020000/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K228008/annotation2020000/range2020000/1
type: Range
persistent identity: range2020000
display id: range2020000
version: 1
top level: BBa_K228008
direction: direction/forward
start: 4
end: 9
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2020001
display id: annotation2020001
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020001/range2020001/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K228008/annotation2020001/range2020001/1
type: Range
persistent identity: range2020001
display id: range2020001
version: 1
top level: BBa_K228008
direction: direction/forward
start: 27
end: 32
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2020003
display id: annotation2020003
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020003/range2020003/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K228008/annotation2020003/range2020003/1
type: Range
persistent identity: range2020003
display id: range2020003
version: 1
top level: BBa_K228008
direction: direction/reverse
start: 47
end: 58
orientation: inline

BBa_K228006
type: SequenceAnnotation
persistent identity: annotation2020015
display id: annotation2020015
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020015/range2020015/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K228008/annotation2020015/range2020015/1
type: Range
persistent identity: range2020015
display id: range2020015
version: 1
top level: BBa_K228008
direction: direction/reverse
start: 47
end: 1141
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2020004
display id: annotation2020004
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020004/range2020004/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_K228008/annotation2020004/range2020004/1
type: Range
persistent identity: range2020004
display id: range2020004
version: 1
top level: BBa_K228008
direction: direction/forward
start: 51
end: 54
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation2020006
display id: annotation2020006
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020006/range2020006/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K228008/annotation2020006/range2020006/1
type: Range
persistent identity: range2020006
display id: range2020006
version: 1
top level: BBa_K228008
direction: direction/forward
start: 65
end: 940
orientation: inline

BBa_C0080
type: SequenceAnnotation
persistent identity: annotation2020005
display id: annotation2020005
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020005/range2020005/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K228008/annotation2020005/range2020005/1
type: Range
persistent identity: range2020005
display id: range2020005
version: 1
top level: BBa_K228008
direction: direction/reverse
start: 65
end: 979
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation2020007
display id: annotation2020007
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020007/range2020007/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_K228008/annotation2020007/range2020007/1
type: Range
persistent identity: range2020007
display id: range2020007
version: 1
top level: BBa_K228008
direction: direction/forward
start: 941
end: 973
orientation: inline

barcode
type: SequenceAnnotation
persistent identity: annotation2020008
display id: annotation2020008
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020008/range2020008/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K228008/annotation2020008/range2020008/1
type: Range
persistent identity: range2020008
display id: range2020008
version: 1
top level: BBa_K228008
direction: direction/reverse
start: 980
end: 1004
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2020009
display id: annotation2020009
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020009/range2020009/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K228008/annotation2020009/range2020009/1
type: Range
persistent identity: range2020009
display id: range2020009
version: 1
top level: BBa_K228008
direction: direction/forward
start: 1013
end: 1092
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2020010
display id: annotation2020010
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020010/range2020010/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_K228008/annotation2020010/range2020010/1
type: Range
persistent identity: range2020010
display id: range2020010
version: 1
top level: BBa_K228008
direction: direction/forward
start: 1024
end: 1067
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2020011
display id: annotation2020011
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020011/range2020011/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K228008/annotation2020011/range2020011/1
type: Range
persistent identity: range2020011
display id: range2020011
version: 1
top level: BBa_K228008
direction: direction/forward
start: 1101
end: 1141
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2020012
display id: annotation2020012
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020012/range2020012/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_K228008/annotation2020012/range2020012/1
type: Range
persistent identity: range2020012
display id: range2020012
version: 1
top level: BBa_K228008
direction: direction/forward
start: 1108
end: 1127
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation2020013
display id: annotation2020013
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020013/range2020013/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_K228008/annotation2020013/range2020013/1
type: Range
persistent identity: range2020013
display id: range2020013
version: 1
top level: BBa_K228008
direction: direction/forward
start: 1128
end: 1141
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2020014
display id: annotation2020014
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020014/range2020014/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K228008/annotation2020014/range2020014/1
type: Range
persistent identity: range2020014
display id: range2020014
version: 1
top level: BBa_K228008
direction: direction/forward
start: 1134
end: 1134
orientation: inline

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation2020016
display id: annotation2020016
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020016/range2020016/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K228008/annotation2020016/range2020016/1
type: Range
persistent identity: range2020016
display id: range2020016
version: 1
top level: BBa_K228008
direction: direction/reverse
start: 1150
end: 1279
orientation: inline

https://synbiohub.org/public/igem/BBa_K228866_sequence/1
type: Sequence
persistent identity: BBa K228866 sequence
display id: BBa_K228866_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggctctagtagtgatctacactagcactatcagtgttattaagctactaaagcgtagttttcgtcgtttgcagctgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatactcgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtagtgctcaaaagcagcttcgcctggctgatgcgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaggcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaacccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcagacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccattggcctcaatcggcgttaaacccgccaccagatgggcgttaaacgagtatcccggcagcaggggatcattttgcgcttcagccatctagtatttctcctctttctctagtatgtttcattatggtgaaagttggaacctcttacgtgcctactagagacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagcaattcggagagatgccggagcggctgaacggaccggtctctaaaaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactacagatccttagcgaaagctaaggattttttttaagcttactagagtcaatccgtaaacaggtcaaacatcagttgccgcaaccaaatattggctaggtccttgtggtacttcgcatgccagaacatgttgatggctatttcaggcaagacgactgggtgcggcaaggcgcttaggccgaagggctccacgcagcagtcggctaaacgtatcggcacagtggcgagcagatcggtgcgctggaggatgtggccaacggcggcgaagtgcggcacttccagacggatgtcgcgccggatgccgacccgtgtcatgtacgtgtccacctcgccgtggccggtgccagcggcgatgacacgcacgtggccgtaggaacagaagcgctccagagtcaggggttcgcgggtgactggatggtccttgcgacataggcacacgtagtgattctggagcagccggcgctgaaagaagccagtttgcagattgggaagcaggcccacggccaagtccacggttccgttctgcaaggcctgcatcaggctcatcgaactgtcgcgcaccgtactgatcacgcaattgggggcctggtgagccagcacatccatcagccgcggca…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K228865_sequence/1
type: Sequence
persistent identity: BBa K228865 sequence
display id: BBa_K228865_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcaatccgtaaacaggtcaaacatcagttgccgcaaccaaatattggctaggtccttgtggtacttcgcatgccagaacatgttgatggctatttcaggcaagacgactgggtgcggcaaggcgcttaggccgaagggctccacgcagcagtcggctaaacgtatcggcacagtggcgagcagatcggtgcgctggaggatgtggccaacggcggcgaagtgcggcacttccagacggatgtcgcgccggatgccgacccgtgtcatgtacgtgtccacctcgccgtggccggtgccagcggcgatgacacgcacgtggccgtaggaacagaagcgctccagagtcaggggttcgcgggtgactggatggtccttgcgacataggcacacgtagtgattctggagcagccggcgctgaaagaagccagtttgcagattgggaagcaggcccacggccaagtccacggttccgttctgcaaggcctgcatcaggctcatcgaactgtcgcgcaccgtactgatcacgcaattgggggcctggtgagccagcacatccatcagccgcggcatgaagtagatctcgccaatgtcggtcatggccagggtgaaggtacgctcgctggtcagcggatcgaagctttcatggtgctgtagggcgttgcgcagtgcgtgcatggccgaagtgacgggctcggccagatgcgcggcatagggtgtgggttccattccctgatgtgtgcgcacgaagagtgggtcctgtagcgaggtgcgcaggcgtttcagcgcattgctcacggcaggctgggtcaggcccaggttctccgcagtgatagagacgcgtctgtcgaccagcaactggttgaacaccaccagcaggtttaaatccaggtcacgcagttccatggggcctcgcttgggttattgctggtgcccggccgggcgcaatattcatgttgatgatttattatatatcgagtggtgtatttatcaatattgtttgctccgttatcgttattaacaagtcatcaataaagccatcacgagtaccatagtactagagaaagaggagaaatactagatgcgctgccctttctgtcgtcattaggcgcatacccgcaccagccggtatgtgagtgacaatgtcaaagaaagttatctccagtgccagaatatttactgttcggcgacatttaaaacgcatgagtcaatttgtgccgtgattcgttctccggtcacggaggaaaaaccagcaccggcaagcacagcaccggctgttgtccgaaaagttaaaggctgttacagctcaccattcaaccattaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K228012_sequence/1
type: Sequence
persistent identity: BBa K228012 sequence
display id: BBa_K228012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggctctagtagtgatctacactagcactatcagtgttattaagctactaaagcgtagttttcgtcgtttgcagctgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatactcgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtagtgctcaaaagcagcttcgcctggctgatgcgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaggcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaacccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcagacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccattggcctcaatcggcgttaaacccgccaccagatgggcgttaaacgagtatcccggcagcaggggatcattttgcgcttcagccatctagtatttctcctctttctctagtatgtttcattatggtgaaagttggaacctcttacgtgcctactagagacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagcaattcggagagatgccggagcggctgaacggaccggtctctaaaaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactacagatccttagcgaaagctaaggattttttttaagct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K228001_sequence/1
type: Sequence
persistent identity: BBa K228001 sequence
display id: BBa_K228001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caattcggagagatgccggagcggctgaacggaccggtctctaaaaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactacagatccttagcgaaagctaaggattttttttaagct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K228009_sequence/1
type: Sequence
persistent identity: BBa K228009 sequence
display id: BBa_K228009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggctctagtagtgatctacactagcactatcagtgttattaagctactaaagcgtagttttcgtcgtttgcagctgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatactcgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtagtgctcaaaagcagcttcgcctggctgatgcgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaggcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaacccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcagacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccattggcctcaatcggcgttaaacccgccaccagatgggcgttaaacgagtatcccggcagcaggggatcattttgcgcttcagccatctagtatttctcctctttctctagtatgtttcattatggtgaaagttggaacctcttacgtgcctactagagacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K228004_sequence/1
type: Sequence
persistent identity: BBa K228004 sequence
display id: BBa_K228004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcaatccgtaaacaggtcaaacatcagttgccgcaaccaaatattggctaggtccttgtggtacttcgcatgccagaacatgttgatggctatttcaggcaagacgactgggtgcggcaaggcgcttaggccgaagggctccacgcagcagtcggctaaacgtatcggcacagtggcgagcagatcggtgcgctggaggatgtggccaacggcggcgaagtgcggcacttccagacggatgtcgcgccggatgccgacccgtgtcatgtacgtgtccacctcgccgtggccggtgccagcggcgatgacacgcacgtggccgtaggaacagaagcgctccagagtcaggggttcgcgggtgactggatggtccttgcgacataggcacacgtagtgattctggagcagccggcgctgaaagaagccagtttgcagattgggaagcaggcccacggccaagtccacggttccgttctgcaaggcctgcatcaggctcatcgaactgtcgcgcaccgtactgatcacgcaattgggggcctggtgagccagcacatccatcagccgcggcatgaagtagatctcgccaatgtcggtcatggccagggtgaaggtacgctcgctggtcagcggatcgaagctttcatggtgctgtagggcgttgcgcagtgcgtgcatggccgaagtgacgggctcggccagatgcgcggcatagggtgtgggttccattccctgatgtgtgcgcacgaagagtgggtcctgtagcgaggtgcgcaggcgtttcagcgcattgctcacggcaggctgggtcaggcccaggttctccgcagtgatagagacgcgtctgtcgaccagcaactggttgaacaccaccagcaggtttaaatccaggtcacgcagttccatggggcctcgcttgggttattgctggtgcccggccgggcgcaatattcatgttgatgatttattatatatcgagtggtgtatttatcaatattgtttgctccgttatcgttattaacaagtcatcaataaagccatcacgagtaccatag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K228863_sequence/1
type: Sequence
persistent identity: BBa K228863 sequence
display id: BBa_K228863_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcgctgccctttctgtcgtcattaggcgcatacccgcaccagccggtatgtgagtgacaatgtcaaagaaagttatctccagtgccagaatatttactgttcggcgacatttaaaacgcatgagtcaatttgtgccgtgattcgttctccggtcacggaggaaaaaccagcaccggcaagcacagcaccggctgttgtccgaaaagttaaaggctgttacagctcaccattcaaccattaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I13453_sequence/1
type: Sequence
persistent identity: BBa I13453 sequence
display id: BBa_I13453_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K228008_sequence/1
type: Sequence
persistent identity: BBa K228008 sequence
display id: BBa_K228008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggctctagtagtgatctacactagcactatcagtgttattaagctactaaagcgtagttttcgtcgtttgcagctgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatactcgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtagtgctcaaaagcagcttcgcctggctgatgcgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaggcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaacccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcagacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccattggcctcaatcggcgttaaacccgccaccagatgggcgttaaacgagtatcccggcagcaggggatcattttgcgcttcagccatctagtatttctcctctttctctagtatgtttcattatggtgaaagttggaacctcttacgtgcc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z