LOCUS       BBa_K228867             2366 bp    DNA     linear       11-Jun-2024
DEFINITION  pSB1A2-lacZ screening plasmid
ACCESSION   BBa_K228867
VERSION     BBa_K228867.1  
FEATURES             Location/Qualifiers
     misc_feature    1..35
                     /label=BBa_J23100
     misc_feature    48..51
                     /label=conserved
     misc_feature    44..55
                     /label=BBa_B0034
     misc_feature    62..64
                     /label=START
     CDS             62..295
                     /label=lacZ
     misc_feature    290..295
                     /label=STOP
     misc_feature    62..295
                     /label=BBa_I732006
     misc_feature    303..2366
                     /label=pSB1A2 Backbone
ORIGIN
        1 tactagtttg acggctagct cagtcctagg tacagtgcta gctactagag aaagaggaga
       61 aatactagat gaccatgatt acggattcac tggccgtcgt tttacaacgt cgtgactggg
      121 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
      181 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
      241 aatggcgctt tgcctggttt ccggcaccag aagcggtgcc ggaaagctgg ctggagtaat
      301 aactgcaggc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
      361 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
      421 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
      481 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
      541 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
      601 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
      661 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
      721 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
      781 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
      841 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
      901 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
      961 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     1021 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     1081 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     1141 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     1201 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     1261 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     1321 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     1381 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     1441 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     1501 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     1561 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     1621 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     1681 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     1741 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     1801 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     1861 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     1921 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     1981 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     2041 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     2101 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     2161 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     2221 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
     2281 ggcgtatcac gaggcagaat ttcagataaa aaaaatcctt agctttcgct aaggatgatt
     2341 tctggaattc gcggccgctt ctagag
//