LOCUS BBa_K228869 4117 bp DNA linear 22-Nov-2024 DEFINITION AraC(K228009) + RBS(B0031) + T7ptag(K228000) + terminator(B0015) ACCESSION BBa_K228869 VERSION BBa_K228869.1 FEATURES Location/Qualifiers stem_loop 1024..1067 /label=stem_loop misc_feature 1013..1092 /label=BBa_B0010 terminator 1013..1092 /label=BBa_B0010 stem_loop 1108..1127 /label=T7 TE misc_feature 1134..1134 /label=stop polyA_site 1128..1141 /label=polya misc_feature 1101..1141 /label=BBa_B0012 terminator 1101..1141 /label=BBa_B0012 promoter 1150..1279 /label=BBa_I13453 misc_feature 1..38 /label=P(Cat) promoter 4..9 /label=-35 promoter 27..32 /label=-10 misc_feature 1..38 /label=BBa_I14033 misc_feature 47..58 /label=BBa_B0034 misc_feature 51..54 /label=conserved misc_feature 65..979 /label=BBa_C0080 CDS 65..940 /label=araC misc_feature 941..973 /label=LVA misc_feature 980..1004 /label=barcode misc_feature 1013..1092 /label=BBa_B0010 stem_loop 1024..1067 /label=stem_loop misc_feature 1101..1141 /label=BBa_B0012 stem_loop 1108..1127 /label=T7 TE polyA_site 1128..1141 /label=polya misc_feature 1134..1134 /label=stop misc_feature 47..1141 /label=BBa_K228006 misc_feature 1150..1279 /label=BBa_I13453 misc_feature 1..1141 /label=BBa_K228008 promoter 1150..1279 /label=BBa_I13453 promoter 1..1279 /label=BBa_K228009 misc_feature 1294..1297 /label=conserved RBS 1288..1301 /label=BBa_B0031 CDS 1308..3980 /label=cds CDS 1308..3980 /label=BBa_K228000 stem_loop 4000..4043 /label=stem_loop misc_feature 3989..4068 /label=BBa_B0010 terminator 3989..4068 /label=BBa_B0010 stem_loop 4084..4103 /label=T7 TE misc_feature 4110..4110 /label=stop polyA_site 4104..4117 /label=polya misc_feature 4077..4117 /label=BBa_B0012 terminator 4077..4117 /label=BBa_B0012 terminator 3989..4117 /label=BBa_B0015 ORIGIN 1 tataaacgca gaaaggccca cccgaaggtg agccagtgtg actctagtag agagcgttca 61 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt 121 gatgcctggc tctagtagtg atctacacta gcactatcag tgttattaag ctactaaagc 181 gtagttttcg tcgtttgcag ctgacaactt gacggctaca tcattcactt tttcttcaca 241 accggcacgg aactcgctcg ggctggcccc ggtgcatttt ttaaatactc gcgagaaata 301 gagttgatcg tcaaaaccaa cattgcgacc gacggtggcg ataggcatcc gggtagtgct 361 caaaagcagc ttcgcctggc tgatgcgttg gtcctcgcgc cagcttaaga cgctaatccc 421 taactgctgg cggaaaagat gtgacagacg cgacggcgac aggcaaacat gctgtgcgac 481 gctggcgata tcaaaattgc tgtctgccag gtgatcgctg atgtactgac aagcctcgcg 541 tacccgatta tccatcggtg gatggagcga ctcgttaatc gcttccatgc gccgcagtaa 601 caattgctca agcagattta tcgccagcag ctccgaatag cgcccttccc cttgcccggc 661 gttaatgatt tgcccaaaca ggtcgctgaa atgcggctgg tgcgcttcat ccgggcgaaa 721 gaaacccgta ttggcaaata ttgacggcca gttaagccat tcatgccagt aggcgcgcgg 781 acgaaagtaa acccactggt gataccattc gcgagcctcc ggatgacgac cgtagtgatg 841 aatctctcct ggcgggaaca gcaaaatatc acccggtcgg cagacaaatt ctcgtccctg 901 atttttcacc accccctgac cgcgaatggt gagattgaga atataacctt tcattcccag 961 cggtcggtcg ataaaaaaat cgagataacc attggcctca atcggcgtta aacccgccac 1021 cagatgggcg ttaaacgagt atcccggcag caggggatca ttttgcgctt cagccatcta 1081 gtatttctcc tctttctcta gtatgtttca ttatggtgaa agttggaacc tcttacgtgc 1141 ctactagaga cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat 1201 ccataagatt agcggatcct acctgacgct ttttatcgca actctctact gtttctccat 1261 accgtttttt tgggctagct actagagtca cacaggaaac ctactagatg accatgatta 1321 ccgtgcacta gaataccatt aacattgcta agaacgactt ctctgacatc gaactggctg 1381 ctatcccgtt caacactctg gctgaccatt acggtgagcg tttagctcgc gaacagttgg 1441 cccttgagca tgagtcttac gagatgggtg aagcacgctt ccgcaagatg tttgagcgtc 1501 aacttaaagc tggtgaggtt gcggataacg ctgccgccaa gcctctcatc actaccctac 1561 tccctaagat gattgcacgc atcaacgact ggtttgagga agtgaaagct aagcgcggca 1621 agcgcccgac agccttccag ttcctgtagg aaatcaagcc ggaagccgta gcgtacatca 1681 ccattaagac cactctggct tgcctaacca gtgctgacaa tacaaccgtt caggctgtag 1741 caagcgcaat cggtcgggcc attgaggacg aggctcgctt cggtcgtatc cgtgaccttg 1801 aagctaagca cttcaagaaa aacgttgagg aacaactcaa caagcgcgta gggcacgtct 1861 acaagaaagc atttatgcaa gttgtcgagg ctgacatgct ctctaagggt ctactcggtg 1921 gcgaggcgtg gtcttcgtgg cataaggaag actctattca tgtaggagta cgctgcatcg 1981 agatgctcat tgagtcaacc ggaatggtta gcttacaccg ccaaaatgct ggcgtagtag 2041 gtcaagactc tgagactatc gaactcgcac ctgaatacgc tgaggctatc gcaacccgtg 2101 caggtgcgct ggctggcatc tctccgatgt tccaaccttg cgtagttcct cctaagccgt 2161 ggactggcat tactggtggt ggctattggg ctaacggtcg tcgtcctctg gcgctggtgc 2221 gtactcacag taagaaagca ctgatgcgct acgaagacgt ttacatgcct gaggtgtaca 2281 aagcgattaa cattgcgcaa aacaccgcat ggaaaatcaa caagaaagtc ctagcggtcg 2341 ccaacgtaat caccaagtgg aagcattgtc cggtcgagga catccctgcg attgagcgtg 2401 aagaactccc gatgaaaccg gaagacatcg acatgaatcc tgaggctctc accgcgtgga 2461 aacgtgctgc cgctgctgtg taccgcaagg acaaggctcg caagtctcgc cgtatcagcc 2521 ttgagttcat gcttgagcaa gccaataagt ttgctaacca taaggccatc tggttccctt 2581 acaacatgga ctggcgcggt cgtgtttacg ctgtgtcaat gttcaacccg caaggtaacg 2641 atatgaccaa aggactgctt acgctggcga aaggtaaacc aatcggtaag gaaggttact 2701 actggctgaa aatccacggt gcaaactgtg cgggtgtcga taaggttccg ttccctgagc 2761 gcatcaagtt cattgaggaa aaccacgaga acatcatggc ttgcgctaag tctccactgg 2821 agaacacttg gtgggctgag caagattctc cgttctgctt ccttgcgttc tgctttgagt 2881 acgctggggt acagcaccac ggcctgagct ataactgctc ccttccgctg gcgtttgacg 2941 ggtcttgctc tggcatccag cacttctccg cgatgctccg agatgaggta ggtggtcgcg 3001 cggttaactt gcttcctagt gaaaccgttc aggacatcta cgggattgtt gctaagaaag 3061 tcaacgagat tctacaagca gacgcaatca atgggaccga taacgaagta gttaccgtga 3121 ccgatgagaa cactggtgaa atctctgaga aagtcaagct gggcactaag gcactggctg 3181 gtcaatggct ggcttacggt gttactcgca gtgtgactaa gcgttcagtc atgacgctgg 3241 cttacgggtc caaagagttc ggcttccgtc aacaagtgct ggaagatacc attcagccag 3301 ctattgattc cggcaagggt ctgatgttca ctcagccgaa tcaggctgct ggatacatgg 3361 ctaagctgat ttgggaatct gtgagcgtga cggtggtagc tgcggttgaa gcaatgaact 3421 ggcttaagtc tgctgctaag ctgctggctg ctgaggtcaa agataagaag actggagaga 3481 ttcttcgcaa gcgttgcgct gtgcattggg taactcctga tggtttccct gtgtggcagg 3541 aatacaagaa gcctattcag acgcgcttga acctgatgtt cctcggtcag ttccgcttac 3601 agcctaccat taacaccaac aaagatagcg agattgatgc acacaaacag gagtctggta 3661 tcgctcctaa ctttgtacac agccaagacg gtagccacct tcgtaagact gtagtgtggg 3721 cacacgagaa gtacggaatc gaatcttttg cactgattca cgactccttc ggtaccattc 3781 cggctgacgc tgcgaacctg ttcaaagcag tgcgcgaaac tatggttgac acatatgagt 3841 cttgtgatgt actggctgat ttctacgacc agttcgctga ccagttgcac gagtctcaat 3901 tggacaaaat gccagcactt ccggctaaag gtaacttgaa cctccgtgac atcttagagt 3961 cggacttcgc gttcgcataa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg 4021 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac 4081 actggctcac cttcgggtgg gcctttctgc gtttata //