LOCUS BBa_K228870 3379 bp DNA linear 01-Nov-2024 DEFINITION AND GATE AraC+RBS(B0030)+T7ptag+Sal+SupD ACCESSION BBa_K228870 VERSION BBa_K228870.1 FEATURES Location/Qualifiers CDS 1..903 /label=NahR promoter 904..993 /label=Pr promoter promoter 982..1050 /label=pSal promoter 1..1052 /label=BBa_K228004 misc_feature 1061..1196 /label=BBa_K228001 stem_loop 1216..1259 /label=stem_loop misc_feature 1205..1284 /label=BBa_B0010 terminator 1205..1284 /label=BBa_B0010 stem_loop 1300..1319 /label=T7 TE misc_feature 1326..1326 /label=stop polyA_site 1320..1333 /label=polya misc_feature 1293..1333 /label=BBa_B0012 terminator 1293..1333 /label=BBa_B0012 misc_feature 1..1333 /label=BBa_K228851 CDS 1342..2244 /label=NahR promoter 2245..2334 /label=Pr promoter promoter 2323..2391 /label=pSal promoter 1342..2393 /label=BBa_K228004 promoter 2402..2493 /label=PO promoter 2402..2493 /label=BBa_I746361 RBS 2502..2516 /label=RBS-1\Strong RBS 2509..2513 /label=RBS misc_feature 2502..2516 /label=BBa_B0030 RBS 2502..2516 /label=BBa_B0030 CDS 2523..3242 /label=GFP protein CDS 2523..3242 /label=BBa_E0040 stem_loop 3262..3305 /label=stem_loop misc_feature 3251..3330 /label=BBa_B0010 terminator 3251..3330 /label=BBa_B0010 stem_loop 3346..3365 /label=T7 TE misc_feature 3372..3372 /label=stop polyA_site 3366..3379 /label=polya misc_feature 3339..3379 /label=BBa_B0012 terminator 3339..3379 /label=BBa_B0012 misc_feature 1342..3379 /label=BBa_K228861 ORIGIN 1 tcaatccgta aacaggtcaa acatcagttg ccgcaaccaa atattggcta ggtccttgtg 61 gtacttcgca tgccagaaca tgttgatggc tatttcaggc aagacgactg ggtgcggcaa 121 ggcgcttagg ccgaagggct ccacgcagca gtcggctaaa cgtatcggca cagtggcgag 181 cagatcggtg cgctggagga tgtggccaac ggcggcgaag tgcggcactt ccagacggat 241 gtcgcgccgg atgccgaccc gtgtcatgta cgtgtccacc tcgccgtggc cggtgccagc 301 ggcgatgaca cgcacgtggc cgtaggaaca gaagcgctcc agagtcaggg gttcgcgggt 361 gactggatgg tccttgcgac ataggcacac gtagtgattc tggagcagcc ggcgctgaaa 421 gaagccagtt tgcagattgg gaagcaggcc cacggccaag tccacggttc cgttctgcaa 481 ggcctgcatc aggctcatcg aactgtcgcg caccgtactg atcacgcaat tgggggcctg 541 gtgagccagc acatccatca gccgcggcat gaagtagatc tcgccaatgt cggtcatggc 601 cagggtgaag gtacgctcgc tggtcagcgg atcgaagctt tcatggtgct gtagggcgtt 661 gcgcagtgcg tgcatggccg aagtgacggg ctcggccaga tgcgcggcat agggtgtggg 721 ttccattccc tgatgtgtgc gcacgaagag tgggtcctgt agcgaggtgc gcaggcgttt 781 cagcgcattg ctcacggcag gctgggtcag gcccaggttc tccgcagtga tagagacgcg 841 tctgtcgacc agcaactggt tgaacaccac cagcaggttt aaatccaggt cacgcagttc 901 catggggcct cgcttgggtt attgctggtg cccggccggg cgcaatattc atgttgatga 961 tttattatat atcgagtggt gtatttatca atattgtttg ctccgttatc gttattaaca 1021 agtcatcaat aaagccatca cgagtaccat agtactagag caattcggag agatgccgga 1081 gcggctgaac ggaccggtct ctaaaaccgg agtaggggca actctaccgg gggttcaaat 1141 ccccctctct ccgccactac agatccttag cgaaagctaa ggattttttt taagcttact 1201 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 1261 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct 1321 ttctgcgttt atatactaga gtcaatccgt aaacaggtca aacatcagtt gccgcaacca 1381 aatattggct aggtccttgt ggtacttcgc atgccagaac atgttgatgg ctatttcagg 1441 caagacgact gggtgcggca aggcgcttag gccgaagggc tccacgcagc agtcggctaa 1501 acgtatcggc acagtggcga gcagatcggt gcgctggagg atgtggccaa cggcggcgaa 1561 gtgcggcact tccagacgga tgtcgcgccg gatgccgacc cgtgtcatgt acgtgtccac 1621 ctcgccgtgg ccggtgccag cggcgatgac acgcacgtgg ccgtaggaac agaagcgctc 1681 cagagtcagg ggttcgcggg tgactggatg gtccttgcga cataggcaca cgtagtgatt 1741 ctggagcagc cggcgctgaa agaagccagt ttgcagattg ggaagcaggc ccacggccaa 1801 gtccacggtt ccgttctgca aggcctgcat caggctcatc gaactgtcgc gcaccgtact 1861 gatcacgcaa ttgggggcct ggtgagccag cacatccatc agccgcggca tgaagtagat 1921 ctcgccaatg tcggtcatgg ccagggtgaa ggtacgctcg ctggtcagcg gatcgaagct 1981 ttcatggtgc tgtagggcgt tgcgcagtgc gtgcatggcc gaagtgacgg gctcggccag 2041 atgcgcggca tagggtgtgg gttccattcc ctgatgtgtg cgcacgaaga gtgggtcctg 2101 tagcgaggtg cgcaggcgtt tcagcgcatt gctcacggca ggctgggtca ggcccaggtt 2161 ctccgcagtg atagagacgc gtctgtcgac cagcaactgg ttgaacacca ccagcaggtt 2221 taaatccagg tcacgcagtt ccatggggcc tcgcttgggt tattgctggt gcccggccgg 2281 gcgcaatatt catgttgatg atttattata tatcgagtgg tgtatttatc aatattgttt 2341 gctccgttat cgttattaac aagtcatcaa taaagccatc acgagtacca tagtactaga 2401 gcgcgccccg cgattcgcta aggtgctgtt gtgtcagtga taagccatcc gggactgatg 2461 gcggaggatg cgcatcgtcg ggaaactgat gcctactaga gattaaagag gagaaatact 2521 agatgcgtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg 2581 gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat gcaacatacg 2641 gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca tggccaacac 2701 ttgtcactac tttcggttat ggtgttcaat gctttgcgag atacccagat catatgaaac 2761 agcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaaaga actatatttt 2821 tcaaagatga cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg 2881 ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt cttggacaca 2941 aattggaata caactataac tcacacaatg tatacatcat ggcagacaaa caaaagaatg 3001 gaatcaaagt taacttcaaa attagacaca acattgaaga tggaagcgtt caactagcag 3061 accattatca acaaaatact ccaattggcg atggccctgt ccttttacca gacaaccatt 3121 acctgtccac acaatctgcc ctttcgaaag atcccaacga aaagagagac cacatggtcc 3181 ttcttgagtt tgtaacagct gctgggatta cacatggcat ggatgaacta tacaaataat 3241 aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt 3301 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt 3361 gggcctttct gcgtttata //