LOCUS       BBa_K228871             3279 bp    DNA     linear       30-Jun-2024
DEFINITION  AND GATE AraC+RBS(B0032)+T7ptag+Sal+SupD
ACCESSION   BBa_K228871
VERSION     BBa_K228871.1  
FEATURES             Location/Qualifiers
     CDS             1..903
                     /label=NahR
     promoter        904..993
                     /label=Pr promoter
     promoter        982..1050
                     /label=pSal
     promoter        1..1052
                     /label=BBa_K228004
     misc_feature    1061..1196
                     /label=BBa_K228001
     stem_loop       1216..1259
                     /label=stem_loop
     misc_feature    1205..1284
                     /label=BBa_B0010
     terminator      1205..1284
                     /label=BBa_B0010
     stem_loop       1300..1319
                     /label=T7 TE
     misc_feature    1326..1326
                     /label=stop
     polyA_site      1320..1333
                     /label=polya
     misc_feature    1293..1333
                     /label=BBa_B0012
     terminator      1293..1333
                     /label=BBa_B0012
     misc_feature    1..1333
                     /label=BBa_K228851
     CDS             1342..2244
                     /label=NahR
     promoter        2245..2334
                     /label=Pr promoter
     promoter        2323..2391
                     /label=pSal
     promoter        1342..2393
                     /label=BBa_K228004
     RBS             2402..2416
                     /label=RBS-1\Strong
     RBS             2409..2413
                     /label=RBS
     misc_feature    2402..2416
                     /label=BBa_B0030
     RBS             2402..2416
                     /label=BBa_B0030
     CDS             2423..3142
                     /label=GFP protein
     CDS             2423..3142
                     /label=BBa_E0040
     stem_loop       3162..3205
                     /label=stem_loop
     misc_feature    3151..3230
                     /label=BBa_B0010
     terminator      3151..3230
                     /label=BBa_B0010
     stem_loop       3246..3265
                     /label=T7 TE
     misc_feature    3272..3272
                     /label=stop
     polyA_site      3266..3279
                     /label=polya
     misc_feature    3239..3279
                     /label=BBa_B0012
     terminator      3239..3279
                     /label=BBa_B0012
     misc_feature    1342..3279
                     /label=BBa_K228862
ORIGIN
        1 tcaatccgta aacaggtcaa acatcagttg ccgcaaccaa atattggcta ggtccttgtg
       61 gtacttcgca tgccagaaca tgttgatggc tatttcaggc aagacgactg ggtgcggcaa
      121 ggcgcttagg ccgaagggct ccacgcagca gtcggctaaa cgtatcggca cagtggcgag
      181 cagatcggtg cgctggagga tgtggccaac ggcggcgaag tgcggcactt ccagacggat
      241 gtcgcgccgg atgccgaccc gtgtcatgta cgtgtccacc tcgccgtggc cggtgccagc
      301 ggcgatgaca cgcacgtggc cgtaggaaca gaagcgctcc agagtcaggg gttcgcgggt
      361 gactggatgg tccttgcgac ataggcacac gtagtgattc tggagcagcc ggcgctgaaa
      421 gaagccagtt tgcagattgg gaagcaggcc cacggccaag tccacggttc cgttctgcaa
      481 ggcctgcatc aggctcatcg aactgtcgcg caccgtactg atcacgcaat tgggggcctg
      541 gtgagccagc acatccatca gccgcggcat gaagtagatc tcgccaatgt cggtcatggc
      601 cagggtgaag gtacgctcgc tggtcagcgg atcgaagctt tcatggtgct gtagggcgtt
      661 gcgcagtgcg tgcatggccg aagtgacggg ctcggccaga tgcgcggcat agggtgtggg
      721 ttccattccc tgatgtgtgc gcacgaagag tgggtcctgt agcgaggtgc gcaggcgttt
      781 cagcgcattg ctcacggcag gctgggtcag gcccaggttc tccgcagtga tagagacgcg
      841 tctgtcgacc agcaactggt tgaacaccac cagcaggttt aaatccaggt cacgcagttc
      901 catggggcct cgcttgggtt attgctggtg cccggccggg cgcaatattc atgttgatga
      961 tttattatat atcgagtggt gtatttatca atattgtttg ctccgttatc gttattaaca
     1021 agtcatcaat aaagccatca cgagtaccat agtactagag caattcggag agatgccgga
     1081 gcggctgaac ggaccggtct ctaaaaccgg agtaggggca actctaccgg gggttcaaat
     1141 ccccctctct ccgccactac agatccttag cgaaagctaa ggattttttt taagcttact
     1201 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
     1261 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct
     1321 ttctgcgttt atatactaga gtcaatccgt aaacaggtca aacatcagtt gccgcaacca
     1381 aatattggct aggtccttgt ggtacttcgc atgccagaac atgttgatgg ctatttcagg
     1441 caagacgact gggtgcggca aggcgcttag gccgaagggc tccacgcagc agtcggctaa
     1501 acgtatcggc acagtggcga gcagatcggt gcgctggagg atgtggccaa cggcggcgaa
     1561 gtgcggcact tccagacgga tgtcgcgccg gatgccgacc cgtgtcatgt acgtgtccac
     1621 ctcgccgtgg ccggtgccag cggcgatgac acgcacgtgg ccgtaggaac agaagcgctc
     1681 cagagtcagg ggttcgcggg tgactggatg gtccttgcga cataggcaca cgtagtgatt
     1741 ctggagcagc cggcgctgaa agaagccagt ttgcagattg ggaagcaggc ccacggccaa
     1801 gtccacggtt ccgttctgca aggcctgcat caggctcatc gaactgtcgc gcaccgtact
     1861 gatcacgcaa ttgggggcct ggtgagccag cacatccatc agccgcggca tgaagtagat
     1921 ctcgccaatg tcggtcatgg ccagggtgaa ggtacgctcg ctggtcagcg gatcgaagct
     1981 ttcatggtgc tgtagggcgt tgcgcagtgc gtgcatggcc gaagtgacgg gctcggccag
     2041 atgcgcggca tagggtgtgg gttccattcc ctgatgtgtg cgcacgaaga gtgggtcctg
     2101 tagcgaggtg cgcaggcgtt tcagcgcatt gctcacggca ggctgggtca ggcccaggtt
     2161 ctccgcagtg atagagacgc gtctgtcgac cagcaactgg ttgaacacca ccagcaggtt
     2221 taaatccagg tcacgcagtt ccatggggcc tcgcttgggt tattgctggt gcccggccgg
     2281 gcgcaatatt catgttgatg atttattata tatcgagtgg tgtatttatc aatattgttt
     2341 gctccgttat cgttattaac aagtcatcaa taaagccatc acgagtacca tagtactaga
     2401 gattaaagag gagaaatact agatgcgtaa aggagaagaa cttttcactg gagttgtccc
     2461 aattcttgtt gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg
     2521 tgaaggtgat gcaacatacg gaaaacttac ccttaaattt atttgcacta ctggaaaact
     2581 acctgttcca tggccaacac ttgtcactac tttcggttat ggtgttcaat gctttgcgag
     2641 atacccagat catatgaaac agcatgactt tttcaagagt gccatgcccg aaggttatgt
     2701 acaggaaaga actatatttt tcaaagatga cgggaactac aagacacgtg ctgaagtcaa
     2761 gtttgaaggt gatacccttg ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga
     2821 tggaaacatt cttggacaca aattggaata caactataac tcacacaatg tatacatcat
     2881 ggcagacaaa caaaagaatg gaatcaaagt taacttcaaa attagacaca acattgaaga
     2941 tggaagcgtt caactagcag accattatca acaaaatact ccaattggcg atggccctgt
     3001 ccttttacca gacaaccatt acctgtccac acaatctgcc ctttcgaaag atcccaacga
     3061 aaagagagac cacatggtcc ttcttgagtt tgtaacagct gctgggatta cacatggcat
     3121 ggatgaacta tacaaataat aatactagag ccaggcatca aataaaacga aaggctcagt
     3181 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
     3241 acactggctc accttcgggt gggcctttct gcgtttata
//