LOCUS       BBa_K228872             1582 bp    DNA     linear       22-Dec-2024
DEFINITION  AND GATE AraC+RBS(B0034)+T7ptag+Sal+SupD
ACCESSION   BBa_K228872
VERSION     BBa_K228872.1  
FEATURES             Location/Qualifiers
     CDS             1..903
                     /label=NahR
     promoter        904..993
                     /label=Pr promoter
     promoter        982..1050
                     /label=pSal
     promoter        1..1052
                     /label=BBa_K228004
     misc_feature    1061..1196
                     /label=BBa_K228001
     stem_loop       1216..1259
                     /label=stem_loop
     misc_feature    1205..1284
                     /label=BBa_B0010
     terminator      1205..1284
                     /label=BBa_B0010
     stem_loop       1300..1319
                     /label=T7 TE
     misc_feature    1326..1326
                     /label=stop
     polyA_site      1320..1333
                     /label=polya
     misc_feature    1293..1333
                     /label=BBa_B0012
     terminator      1293..1333
                     /label=BBa_B0012
     misc_feature    1..1333
                     /label=BBa_K228851
     CDS             1340..1582
                     /label=cds
     misc_feature    1364..1366
                     /label=TAG amber mutation
     CDS             1340..1582
                     /label=BBa_K228863
ORIGIN
        1 tcaatccgta aacaggtcaa acatcagttg ccgcaaccaa atattggcta ggtccttgtg
       61 gtacttcgca tgccagaaca tgttgatggc tatttcaggc aagacgactg ggtgcggcaa
      121 ggcgcttagg ccgaagggct ccacgcagca gtcggctaaa cgtatcggca cagtggcgag
      181 cagatcggtg cgctggagga tgtggccaac ggcggcgaag tgcggcactt ccagacggat
      241 gtcgcgccgg atgccgaccc gtgtcatgta cgtgtccacc tcgccgtggc cggtgccagc
      301 ggcgatgaca cgcacgtggc cgtaggaaca gaagcgctcc agagtcaggg gttcgcgggt
      361 gactggatgg tccttgcgac ataggcacac gtagtgattc tggagcagcc ggcgctgaaa
      421 gaagccagtt tgcagattgg gaagcaggcc cacggccaag tccacggttc cgttctgcaa
      481 ggcctgcatc aggctcatcg aactgtcgcg caccgtactg atcacgcaat tgggggcctg
      541 gtgagccagc acatccatca gccgcggcat gaagtagatc tcgccaatgt cggtcatggc
      601 cagggtgaag gtacgctcgc tggtcagcgg atcgaagctt tcatggtgct gtagggcgtt
      661 gcgcagtgcg tgcatggccg aagtgacggg ctcggccaga tgcgcggcat agggtgtggg
      721 ttccattccc tgatgtgtgc gcacgaagag tgggtcctgt agcgaggtgc gcaggcgttt
      781 cagcgcattg ctcacggcag gctgggtcag gcccaggttc tccgcagtga tagagacgcg
      841 tctgtcgacc agcaactggt tgaacaccac cagcaggttt aaatccaggt cacgcagttc
      901 catggggcct cgcttgggtt attgctggtg cccggccggg cgcaatattc atgttgatga
      961 tttattatat atcgagtggt gtatttatca atattgtttg ctccgttatc gttattaaca
     1021 agtcatcaat aaagccatca cgagtaccat agtactagag caattcggag agatgccgga
     1081 gcggctgaac ggaccggtct ctaaaaccgg agtaggggca actctaccgg gggttcaaat
     1141 ccccctctct ccgccactac agatccttag cgaaagctaa ggattttttt taagcttact
     1201 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
     1261 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct
     1321 ttctgcgttt atatactaga tgcgctgccc tttctgtcgt cattaggcgc atacccgcac
     1381 cagccggtat gtgagtgaca atgtcaaaga aagttatctc cagtgccaga atatttactg
     1441 ttcggcgaca tttaaaacgc atgagtcaat ttgtgccgtg attcgttctc cggtcacgga
     1501 ggaaaaacca gcaccggcaa gcacagcacc ggctgttgtc cgaaaagtta aaggctgtta
     1561 cagctcacca ttcaaccatt aa
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