LOCUS BBa_K228875 4093 bp DNA linear 30-Jun-2024 DEFINITION AND GATE AraC+RBS(J61127)+T7ptag+Sal+SupD ACCESSION BBa_K228875 VERSION BBa_K228875.1 FEATURES Location/Qualifiers CDS 1..903 /label=NahR promoter 904..993 /label=Pr promoter promoter 982..1050 /label=pSal promoter 1..1052 /label=BBa_K228004 misc_feature 1061..1196 /label=BBa_K228001 stem_loop 1216..1259 /label=stem_loop misc_feature 1205..1284 /label=BBa_B0010 terminator 1205..1284 /label=BBa_B0010 stem_loop 1300..1319 /label=T7 TE misc_feature 1326..1326 /label=stop polyA_site 1320..1333 /label=polya misc_feature 1293..1333 /label=BBa_B0012 terminator 1293..1333 /label=BBa_B0012 misc_feature 1..1333 /label=BBa_K228851 stem_loop 2365..2408 /label=stem_loop misc_feature 2354..2433 /label=BBa_B0010 terminator 2354..2433 /label=BBa_B0010 stem_loop 2449..2468 /label=T7 TE misc_feature 2475..2475 /label=stop polyA_site 2469..2482 /label=polya misc_feature 2442..2482 /label=BBa_B0012 terminator 2442..2482 /label=BBa_B0012 misc_feature 1392..1395 /label=conserved RBS 1388..1399 /label=BBa_B0034 promoter 2491..2620 /label=BBa_I13453 misc_feature 1342..1379 /label=P(Cat) promoter 1345..1350 /label=-35 promoter 1368..1373 /label=-10 misc_feature 1342..1379 /label=BBa_I14033 misc_feature 1388..1399 /label=BBa_B0034 misc_feature 1392..1395 /label=conserved misc_feature 1406..2320 /label=BBa_C0080 CDS 1406..2281 /label=araC misc_feature 2282..2314 /label=LVA misc_feature 2321..2345 /label=barcode misc_feature 2354..2433 /label=BBa_B0010 stem_loop 2365..2408 /label=stem_loop misc_feature 2442..2482 /label=BBa_B0012 stem_loop 2449..2468 /label=T7 TE polyA_site 2469..2482 /label=polya misc_feature 2475..2475 /label=stop misc_feature 1388..2482 /label=BBa_K228006 misc_feature 2491..2620 /label=BBa_I13453 misc_feature 1342..2482 /label=BBa_K228008 promoter 2491..2620 /label=BBa_I13453 promoter 1342..2620 /label=BBa_K228009 misc_feature 2629..2764 /label=BBa_K228001 misc_feature 1342..2764 /label=BBa_K228012 CDS 2773..3675 /label=NahR promoter 3676..3765 /label=Pr promoter promoter 3754..3822 /label=pSal promoter 2773..3824 /label=BBa_K228004 misc_feature 3837..3840 /label=conserved RBS 3833..3844 /label=BBa_B0034 CDS 3851..4093 /label=cds misc_feature 3875..3877 /label=TAG amber mutation CDS 3851..4093 /label=BBa_K228863 misc_feature 2773..4093 /label=BBa_K228865 misc_feature 1342..4093 /label=BBa_K228866 ORIGIN 1 tcaatccgta aacaggtcaa acatcagttg ccgcaaccaa atattggcta ggtccttgtg 61 gtacttcgca tgccagaaca tgttgatggc tatttcaggc aagacgactg ggtgcggcaa 121 ggcgcttagg ccgaagggct ccacgcagca gtcggctaaa cgtatcggca cagtggcgag 181 cagatcggtg cgctggagga tgtggccaac ggcggcgaag tgcggcactt ccagacggat 241 gtcgcgccgg atgccgaccc gtgtcatgta cgtgtccacc tcgccgtggc cggtgccagc 301 ggcgatgaca cgcacgtggc cgtaggaaca gaagcgctcc agagtcaggg gttcgcgggt 361 gactggatgg tccttgcgac ataggcacac gtagtgattc tggagcagcc ggcgctgaaa 421 gaagccagtt tgcagattgg gaagcaggcc cacggccaag tccacggttc cgttctgcaa 481 ggcctgcatc aggctcatcg aactgtcgcg caccgtactg atcacgcaat tgggggcctg 541 gtgagccagc acatccatca gccgcggcat gaagtagatc tcgccaatgt cggtcatggc 601 cagggtgaag gtacgctcgc tggtcagcgg atcgaagctt tcatggtgct gtagggcgtt 661 gcgcagtgcg tgcatggccg aagtgacggg ctcggccaga tgcgcggcat agggtgtggg 721 ttccattccc tgatgtgtgc gcacgaagag tgggtcctgt agcgaggtgc gcaggcgttt 781 cagcgcattg ctcacggcag gctgggtcag gcccaggttc tccgcagtga tagagacgcg 841 tctgtcgacc agcaactggt tgaacaccac cagcaggttt aaatccaggt cacgcagttc 901 catggggcct cgcttgggtt attgctggtg cccggccggg cgcaatattc atgttgatga 961 tttattatat atcgagtggt gtatttatca atattgtttg ctccgttatc gttattaaca 1021 agtcatcaat aaagccatca cgagtaccat agtactagag caattcggag agatgccgga 1081 gcggctgaac ggaccggtct ctaaaaccgg agtaggggca actctaccgg gggttcaaat 1141 ccccctctct ccgccactac agatccttag cgaaagctaa ggattttttt taagcttact 1201 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 1261 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct 1321 ttctgcgttt atatactaga gtataaacgc agaaaggccc acccgaaggt gagccagtgt 1381 gactctagta gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtctttcg 1441 actgagcctt tcgttttatt tgatgcctgg ctctagtagt gatctacact agcactatca 1501 gtgttattaa gctactaaag cgtagttttc gtcgtttgca gctgacaact tgacggctac 1561 atcattcact ttttcttcac aaccggcacg gaactcgctc gggctggccc cggtgcattt 1621 tttaaatact cgcgagaaat agagttgatc gtcaaaacca acattgcgac cgacggtggc 1681 gataggcatc cgggtagtgc tcaaaagcag cttcgcctgg ctgatgcgtt ggtcctcgcg 1741 ccagcttaag acgctaatcc ctaactgctg gcggaaaaga tgtgacagac gcgacggcga 1801 caggcaaaca tgctgtgcga cgctggcgat atcaaaattg ctgtctgcca ggtgatcgct 1861 gatgtactga caagcctcgc gtacccgatt atccatcggt ggatggagcg actcgttaat 1921 cgcttccatg cgccgcagta acaattgctc aagcagattt atcgccagca gctccgaata 1981 gcgcccttcc ccttgcccgg cgttaatgat ttgcccaaac aggtcgctga aatgcggctg 2041 gtgcgcttca tccgggcgaa agaaacccgt attggcaaat attgacggcc agttaagcca 2101 ttcatgccag taggcgcgcg gacgaaagta aacccactgg tgataccatt cgcgagcctc 2161 cggatgacga ccgtagtgat gaatctctcc tggcgggaac agcaaaatat cacccggtcg 2221 gcagacaaat tctcgtccct gatttttcac caccccctga ccgcgaatgg tgagattgag 2281 aatataacct ttcattccca gcggtcggtc gataaaaaaa tcgagataac cattggcctc 2341 aatcggcgtt aaacccgcca ccagatgggc gttaaacgag tatcccggca gcaggggatc 2401 attttgcgct tcagccatct agtatttctc ctctttctct agtatgtttc attatggtga 2461 aagttggaac ctcttacgtg cctactagag acattgatta tttgcacggc gtcacacttt 2521 gctatgccat agcattttta tccataagat tagcggatcc tacctgacgc tttttatcgc 2581 aactctctac tgtttctcca taccgttttt ttgggctagc tactagagca attcggagag 2641 atgccggagc ggctgaacgg accggtctct aaaaccggag taggggcaac tctaccgggg 2701 gttcaaatcc ccctctctcc gccactacag atccttagcg aaagctaagg atttttttta 2761 agcttactag agtcaatccg taaacaggtc aaacatcagt tgccgcaacc aaatattggc 2821 taggtccttg tggtacttcg catgccagaa catgttgatg gctatttcag gcaagacgac 2881 tgggtgcggc aaggcgctta ggccgaaggg ctccacgcag cagtcggcta aacgtatcgg 2941 cacagtggcg agcagatcgg tgcgctggag gatgtggcca acggcggcga agtgcggcac 3001 ttccagacgg atgtcgcgcc ggatgccgac ccgtgtcatg tacgtgtcca cctcgccgtg 3061 gccggtgcca gcggcgatga cacgcacgtg gccgtaggaa cagaagcgct ccagagtcag 3121 gggttcgcgg gtgactggat ggtccttgcg acataggcac acgtagtgat tctggagcag 3181 ccggcgctga aagaagccag tttgcagatt gggaagcagg cccacggcca agtccacggt 3241 tccgttctgc aaggcctgca tcaggctcat cgaactgtcg cgcaccgtac tgatcacgca 3301 attgggggcc tggtgagcca gcacatccat cagccgcggc atgaagtaga tctcgccaat 3361 gtcggtcatg gccagggtga aggtacgctc gctggtcagc ggatcgaagc tttcatggtg 3421 ctgtagggcg ttgcgcagtg cgtgcatggc cgaagtgacg ggctcggcca gatgcgcggc 3481 atagggtgtg ggttccattc cctgatgtgt gcgcacgaag agtgggtcct gtagcgaggt 3541 gcgcaggcgt ttcagcgcat tgctcacggc aggctgggtc aggcccaggt tctccgcagt 3601 gatagagacg cgtctgtcga ccagcaactg gttgaacacc accagcaggt ttaaatccag 3661 gtcacgcagt tccatggggc ctcgcttggg ttattgctgg tgcccggccg ggcgcaatat 3721 tcatgttgat gatttattat atatcgagtg gtgtatttat caatattgtt tgctccgtta 3781 tcgttattaa caagtcatca ataaagccat cacgagtacc atagtactag agaaagagga 3841 gaaatactag atgcgctgcc ctttctgtcg tcattaggcg catacccgca ccagccggta 3901 tgtgagtgac aatgtcaaag aaagttatct ccagtgccag aatatttact gttcggcgac 3961 atttaaaacg catgagtcaa tttgtgccgt gattcgttct ccggtcacgg aggaaaaacc 4021 agcaccggca agcacagcac cggctgttgt ccgaaaagtt aaaggctgtt acagctcacc 4081 attcaaccat taa //