LOCUS BBa_K228876 3707 bp DNA linear 01-Nov-2024 DEFINITION AND GATE AraC+RBS(J44001)+T7ptag+Sal+SupD ACCESSION BBa_K228876 VERSION BBa_K228876.1 FEATURES Location/Qualifiers CDS 1..903 /label=NahR promoter 904..993 /label=Pr promoter promoter 982..1050 /label=pSal promoter 1..1052 /label=BBa_K228004 misc_feature 1061..1196 /label=BBa_K228001 stem_loop 1216..1259 /label=stem_loop misc_feature 1205..1284 /label=BBa_B0010 terminator 1205..1284 /label=BBa_B0010 stem_loop 1300..1319 /label=T7 TE misc_feature 1326..1326 /label=stop polyA_site 1320..1333 /label=polya misc_feature 1293..1333 /label=BBa_B0012 terminator 1293..1333 /label=BBa_B0012 misc_feature 1..1333 /label=BBa_K228851 misc_feature 1342..1376 /label=BBa_J23100 misc_feature 1389..1392 /label=conserved misc_feature 1385..1396 /label=BBa_B0034 misc_feature 1403..1405 /label=START CDS 1403..1636 /label=lacZ misc_feature 1631..1636 /label=STOP misc_feature 1403..1636 /label=BBa_I732006 misc_feature 1644..3707 /label=pSB1A2 Backbone misc_feature 1342..3707 /label=BBa_K228867 ORIGIN 1 tcaatccgta aacaggtcaa acatcagttg ccgcaaccaa atattggcta ggtccttgtg 61 gtacttcgca tgccagaaca tgttgatggc tatttcaggc aagacgactg ggtgcggcaa 121 ggcgcttagg ccgaagggct ccacgcagca gtcggctaaa cgtatcggca cagtggcgag 181 cagatcggtg cgctggagga tgtggccaac ggcggcgaag tgcggcactt ccagacggat 241 gtcgcgccgg atgccgaccc gtgtcatgta cgtgtccacc tcgccgtggc cggtgccagc 301 ggcgatgaca cgcacgtggc cgtaggaaca gaagcgctcc agagtcaggg gttcgcgggt 361 gactggatgg tccttgcgac ataggcacac gtagtgattc tggagcagcc ggcgctgaaa 421 gaagccagtt tgcagattgg gaagcaggcc cacggccaag tccacggttc cgttctgcaa 481 ggcctgcatc aggctcatcg aactgtcgcg caccgtactg atcacgcaat tgggggcctg 541 gtgagccagc acatccatca gccgcggcat gaagtagatc tcgccaatgt cggtcatggc 601 cagggtgaag gtacgctcgc tggtcagcgg atcgaagctt tcatggtgct gtagggcgtt 661 gcgcagtgcg tgcatggccg aagtgacggg ctcggccaga tgcgcggcat agggtgtggg 721 ttccattccc tgatgtgtgc gcacgaagag tgggtcctgt agcgaggtgc gcaggcgttt 781 cagcgcattg ctcacggcag gctgggtcag gcccaggttc tccgcagtga tagagacgcg 841 tctgtcgacc agcaactggt tgaacaccac cagcaggttt aaatccaggt cacgcagttc 901 catggggcct cgcttgggtt attgctggtg cccggccggg cgcaatattc atgttgatga 961 tttattatat atcgagtggt gtatttatca atattgtttg ctccgttatc gttattaaca 1021 agtcatcaat aaagccatca cgagtaccat agtactagag caattcggag agatgccgga 1081 gcggctgaac ggaccggtct ctaaaaccgg agtaggggca actctaccgg gggttcaaat 1141 ccccctctct ccgccactac agatccttag cgaaagctaa ggattttttt taagcttact 1201 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 1261 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct 1321 ttctgcgttt atatactaga gtactagttt gacggctagc tcagtcctag gtacagtgct 1381 agctactaga gaaagaggag aaatactaga tgaccatgat tacggattca ctggccgtcg 1441 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac 1501 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac 1561 agttgcgcag cctgaatggc gaatggcgct ttgcctggtt tccggcacca gaagcggtgc 1621 cggaaagctg gctggagtaa taactgcagg cttcctcgct cactgactcg ctgcgctcgg 1681 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag 1741 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc 1801 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca 1861 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt 1921 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc 1981 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc 2041 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc 2101 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact 2161 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg 2221 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta 2281 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca 2341 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa 2401 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg 2461 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc 2521 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg 2581 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat 2641 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg 2701 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa 2761 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca 2821 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc 2881 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt 2941 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa 3001 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat 3061 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct 3121 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga 3181 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag 3241 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga 3301 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca 3361 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg 3421 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc 3481 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag 3541 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca 3601 tgacattaac ctataaaaat aggcgtatca cgaggcagaa tttcagataa aaaaaatcct 3661 tagctttcgc taaggatgat ttctggaatt cgcggccgct tctagag //