BBa_K228876
type: ComponentDefinition
persistent identity: BBa K228876
display id: BBa_K228876
version: 1
was generated by: iGEM to SBOL conversion
description: AND GATE AraC+RBS(J44001)+T7ptag+Sal+SupD
created: 2009-09-21T11:00:00Z
modified: 2015-05-08T01:11:35Z
mutable provenance: It is made up of standard subparts from the partregistry.
owned by: james
owned by: myers
mutable description: This is an AND GATE in Ecoli. Use salicylate to induce the transcription of SupD and use arabinose to induce the transcription of T7ptag. If the SupD tRNA exists, T7ptag can be translated into functional T7 polymerase, and otherwise the translation of T7ptag will come to stop because of some amber stop codon mutations in the T7ptag. The T7 polymerase will activate a T7 promoter. This gate is only one of nine gates(K228870-K228878), which are distinguished by the RBS of T7ptag. For more information: refer to K228000(T7ptag) and K228001(SupD) referrence: Anderson JC, Voigt CA, Arkin AP (2007) Environmental signal integration by a modular AND gate. Mol Syst Biol 3: 133
discontinued: false
dominant: false
experience: experience/Issues
group u list: _353_
m user id: 0
owner id: 4411
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
star: false
creator: Haoqian Zhang
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K228867
component: BBa_K228851
sequence annotation: BBa_K228851
sequence annotation: BBa_K228867
sequence: https://synbiohub.org/public/igem/BBa_K228876_sequence/1

BBa_K228867
type: Component
persistent identity: component2230599
display id: component2230599
version: 1
top level: BBa_K228876
definition: BBa_K228867
access: public

BBa_K228851
type: Component
persistent identity: component2230590
display id: component2230590
version: 1
top level: BBa_K228876
definition: BBa_K228851
access: public

BBa_K228851
type: SequenceAnnotation
persistent identity: annotation2230590
display id: annotation2230590
version: 1
top level: BBa_K228876
location: https://synbiohub.org/public/igem/BBa_K228876/annotation2230590/range2230590/1
component: BBa_K228851

https://synbiohub.org/public/igem/BBa_K228876/annotation2230590/range2230590/1
type: Range
persistent identity: range2230590
display id: range2230590
version: 1
top level: BBa_K228876
direction: direction/reverse
start: 1
end: 1333
orientation: inline

BBa_K228867
type: SequenceAnnotation
persistent identity: annotation2230599
display id: annotation2230599
version: 1
top level: BBa_K228876
location: https://synbiohub.org/public/igem/BBa_K228876/annotation2230599/range2230599/1
component: BBa_K228867

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type: Range
persistent identity: range2230599
display id: range2230599
version: 1
top level: BBa_K228876
direction: direction/reverse
start: 1342
end: 3707
orientation: inline

BBa_K228851
type: ComponentDefinition
persistent identity: BBa K228851
display id: BBa_K228851
version: 1
was generated by: iGEM to SBOL conversion
description: Sal+supD+terminator
created: 2009-09-20T11:00:00Z
modified: 2015-05-08T01:11:34Z
owned by: james
owned by: myers
mutable description: This is a salicylate inducible part and can produce supD, which is a tRNA coding gene and it can be well terminated by the terminator BBa_B0015.
discontinued: false
dominant: false
experience: experience/Works
group u list: _353_
m user id: 0
owner id: 5502
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
star: false
creator: He Siheng
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K228004
component: BBa_B0010
component: BBa_K228001
component: BBa_B0012
sequence annotation: BBa_K228004
sequence annotation: BBa_K228001
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K228851_sequence/1

BBa_K228004
type: Component
persistent identity: component2023158
display id: component2023158
version: 1
top level: BBa_K228851
definition: BBa_K228004
access: public

BBa_B0010
type: Component
persistent identity: component2023160
display id: component2023160
version: 1
top level: BBa_K228851
definition: BBa_B0010
access: public

BBa_K228001
type: Component
persistent identity: component2023159
display id: component2023159
version: 1
top level: BBa_K228851
definition: BBa_K228001
access: public

BBa_B0012
type: Component
persistent identity: component2023162
display id: component2023162
version: 1
top level: BBa_K228851
definition: BBa_B0012
access: public

BBa_K228004
type: SequenceAnnotation
persistent identity: annotation2023158
display id: annotation2023158
version: 1
top level: BBa_K228851
location: https://synbiohub.org/public/igem/BBa_K228851/annotation2023158/range2023158/1
component: BBa_K228004

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type: Range
persistent identity: range2023158
display id: range2023158
version: 1
top level: BBa_K228851
direction: direction/reverse
start: 1
end: 1052
orientation: inline

BBa_K228001
type: SequenceAnnotation
persistent identity: annotation2023159
display id: annotation2023159
version: 1
top level: BBa_K228851
location: https://synbiohub.org/public/igem/BBa_K228851/annotation2023159/range2023159/1
component: BBa_K228001

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type: Range
persistent identity: range2023159
display id: range2023159
version: 1
top level: BBa_K228851
direction: direction/reverse
start: 1061
end: 1196
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2023160
display id: annotation2023160
version: 1
top level: BBa_K228851
location: https://synbiohub.org/public/igem/BBa_K228851/annotation2023160/range2023160/1
component: BBa_B0010

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type: Range
persistent identity: range2023160
display id: range2023160
version: 1
top level: BBa_K228851
direction: direction/forward
start: 1205
end: 1284
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2023162
display id: annotation2023162
version: 1
top level: BBa_K228851
location: https://synbiohub.org/public/igem/BBa_K228851/annotation2023162/range2023162/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K228851/annotation2023162/range2023162/1
type: Range
persistent identity: range2023162
display id: range2023162
version: 1
top level: BBa_K228851
direction: direction/forward
start: 1293
end: 1333
orientation: inline

BBa_K228867
type: ComponentDefinition
persistent identity: BBa K228867
display id: BBa_K228867
version: 1
was generated by: iGEM to SBOL conversion
description: pSB1A2-lacZ screening plasmid
created: 2009-09-21T11:00:00Z
modified: 2015-05-08T01:11:34Z
mutable provenance: part registery 2009 distribution & PKU 2009 igem team
owned by: james
owned by: myers
mutable description: T7 polymerase with an amber mutation coding gene is constructed after AraC protein(reversed sequence) and Pbad promoter which would be triggered by Arabinose. T7 polymerase can work well in the exsitence of SupD tRNA. Besides, the expression of the T7 polymerase can be regulated by RBS.
discontinued: false
dominant: false
experience: experience/Works
group u list: _353_
m user id: 0
owner id: 4411
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: when cut this part for insert and ligate it into a vetor, the insert and vector may have the similar sizes which may disturb the ligation.
star: false
creator: Lin Min
type: DnaRegion
role: SO:0000755
role: partType/Plasmid Backbone
sequence annotation: BBa_J23100
sequence annotation: BBa_B0034
sequence annotation: conserved
sequence annotation: START
sequence annotation: lacZ
sequence annotation: BBa_I732006
sequence annotation: STOP
sequence annotation: pSB1A2 Backbone
sequence: https://synbiohub.org/public/igem/BBa_K228867_sequence/1

BBa_J23100
type: SequenceAnnotation
persistent identity: annotation2055014
display id: annotation2055014
version: 1
top level: BBa_K228867
location: https://synbiohub.org/public/igem/BBa_K228867/annotation2055014/range2055014/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K228867/annotation2055014/range2055014/1
type: Range
persistent identity: range2055014
display id: range2055014
version: 1
top level: BBa_K228867
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2055016
display id: annotation2055016
version: 1
top level: BBa_K228867
location: https://synbiohub.org/public/igem/BBa_K228867/annotation2055016/range2055016/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K228867/annotation2055016/range2055016/1
type: Range
persistent identity: range2055016
display id: range2055016
version: 1
top level: BBa_K228867
direction: direction/reverse
start: 44
end: 55
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2055015
display id: annotation2055015
version: 1
top level: BBa_K228867
location: https://synbiohub.org/public/igem/BBa_K228867/annotation2055015/range2055015/1
role: SO:0000330
role: feature/conserved

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type: Range
persistent identity: range2055015
display id: range2055015
version: 1
top level: BBa_K228867
direction: direction/forward
start: 48
end: 51
orientation: inline

START
type: SequenceAnnotation
persistent identity: annotation2055017
display id: annotation2055017
version: 1
top level: BBa_K228867
location: https://synbiohub.org/public/igem/BBa_K228867/annotation2055017/range2055017/1
role: feature/start
role: SO:0000318

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type: Range
persistent identity: range2055017
display id: range2055017
version: 1
top level: BBa_K228867
direction: direction/forward
start: 62
end: 64
orientation: inline

lacZ
type: SequenceAnnotation
persistent identity: annotation2055018
display id: annotation2055018
version: 1
top level: BBa_K228867
location: https://synbiohub.org/public/igem/BBa_K228867/annotation2055018/range2055018/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K228867/annotation2055018/range2055018/1
type: Range
persistent identity: range2055018
display id: range2055018
version: 1
top level: BBa_K228867
direction: direction/forward
start: 62
end: 295
orientation: inline

BBa_I732006
type: SequenceAnnotation
persistent identity: annotation2055020
display id: annotation2055020
version: 1
top level: BBa_K228867
location: https://synbiohub.org/public/igem/BBa_K228867/annotation2055020/range2055020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K228867/annotation2055020/range2055020/1
type: Range
persistent identity: range2055020
display id: range2055020
version: 1
top level: BBa_K228867
direction: direction/reverse
start: 62
end: 295
orientation: inline

STOP
type: SequenceAnnotation
persistent identity: annotation2055019
display id: annotation2055019
version: 1
top level: BBa_K228867
location: https://synbiohub.org/public/igem/BBa_K228867/annotation2055019/range2055019/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K228867/annotation2055019/range2055019/1
type: Range
persistent identity: range2055019
display id: range2055019
version: 1
top level: BBa_K228867
direction: direction/forward
start: 290
end: 295
orientation: inline

pSB1A2 Backbone
type: SequenceAnnotation
persistent identity: annotation2055021
display id: annotation2055021
version: 1
top level: BBa_K228867
location: https://synbiohub.org/public/igem/BBa_K228867/annotation2055021/range2055021/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K228867/annotation2055021/range2055021/1
type: Range
persistent identity: range2055021
display id: range2055021
version: 1
top level: BBa_K228867
direction: direction/forward
start: 303
end: 2366
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_K228001
type: ComponentDefinition
persistent identity: BBa K228001
display id: BBa_K228001
version: 1
was generated by: iGEM to SBOL conversion
description: SupD-tRNA
created: 2009-08-24T11:00:00Z
modified: 2015-05-08T01:11:32Z
mutable provenance: SupD-tRNA again is from Voigt Lab(UCSF), We standardlize this part by PCR strategy.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The SupD-tRNA part functions as a tRNA, it suppresses the TAG amber stop codon and translate it into a Ser. There is no need to assembly SupD-tRNA with a rbs, since it is not translated. This part, together with the T7ptag part, make an AND Gate, that is, in the presence of both the 2 parts, T7 polymerase is tranlated into a functional polymerase that transcrips a downstream element constructed after a T7 promoter. Which is the output of the AND Gate.
discontinued: false
dominant: false
experience: experience/Works
group u list: _353_
m user id: 0
owner id: 4253
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: There is a PstI site in the original sequence, but luckily it is near the 3' end of this part, so we designed a mutation in our primer and mutated the PstI site.
star: false
creator: Lin Min
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence: https://synbiohub.org/public/igem/BBa_K228001_sequence/1

BBa_K228004
type: ComponentDefinition
persistent identity: BBa K228004
display id: BBa_K228004
version: 1
was generated by: iGEM to SBOL conversion
description: NahR( reverse) - salicylate promoter
created: 2009-09-17T11:00:00Z
modified: 2015-05-08T01:11:32Z
mutable provenance: It is constrcuted by PCR from the plasmid kindly provided by Voigt Lab in UCSF.
owned by: james
owned by: myers
mutable description: This is a salicylate inducible promoter part. The activator protein is right upstream but in a opposite direction of the regulated promoter. In the presence of salicylate, the promoter can be activated.
discontinued: false
dominant: false
experience: experience/Works
group u list: _353_
m user id: 0
owner id: 4253
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: There is no termoinator downstream of the NahR coding sequence, but it has no effect on parts that are in the same direction as the pSal promoter. However, if coding gene is placed upstream of this part and in the same direction as NahR, it may cause unwanted expression.
star: false
creator: Lin Min
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: NahR
sequence annotation: Pr promoter
sequence annotation: pSal
sequence: https://synbiohub.org/public/igem/BBa_K228004_sequence/1

NahR
type: SequenceAnnotation
persistent identity: annotation2075285
display id: annotation2075285
version: 1
top level: BBa_K228004
location: https://synbiohub.org/public/igem/BBa_K228004/annotation2075285/range2075285/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K228004/annotation2075285/range2075285/1
type: Range
persistent identity: range2075285
display id: range2075285
version: 1
top level: BBa_K228004
direction: direction/reverse
start: 1
end: 903
orientation: inline

Pr promoter
type: SequenceAnnotation
persistent identity: annotation2075286
display id: annotation2075286
version: 1
top level: BBa_K228004
location: https://synbiohub.org/public/igem/BBa_K228004/annotation2075286/range2075286/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K228004/annotation2075286/range2075286/1
type: Range
persistent identity: range2075286
display id: range2075286
version: 1
top level: BBa_K228004
direction: direction/reverse
start: 904
end: 993
orientation: inline

pSal
type: SequenceAnnotation
persistent identity: annotation2075287
display id: annotation2075287
version: 1
top level: BBa_K228004
location: https://synbiohub.org/public/igem/BBa_K228004/annotation2075287/range2075287/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K228004/annotation2075287/range2075287/1
type: Range
persistent identity: range2075287
display id: range2075287
version: 1
top level: BBa_K228004
direction: direction/forward
start: 982
end: 1050
orientation: inline

https://synbiohub.org/public/igem/BBa_K228876_sequence/1
type: Sequence
persistent identity: BBa K228876 sequence
display id: BBa_K228876_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcaatccgtaaacaggtcaaacatcagttgccgcaaccaaatattggctaggtccttgtggtacttcgcatgccagaacatgttgatggctatttcaggcaagacgactgggtgcggcaaggcgcttaggccgaagggctccacgcagcagtcggctaaacgtatcggcacagtggcgagcagatcggtgcgctggaggatgtggccaacggcggcgaagtgcggcacttccagacggatgtcgcgccggatgccgacccgtgtcatgtacgtgtccacctcgccgtggccggtgccagcggcgatgacacgcacgtggccgtaggaacagaagcgctccagagtcaggggttcgcgggtgactggatggtccttgcgacataggcacacgtagtgattctggagcagccggcgctgaaagaagccagtttgcagattgggaagcaggcccacggccaagtccacggttccgttctgcaaggcctgcatcaggctcatcgaactgtcgcgcaccgtactgatcacgcaattgggggcctggtgagccagcacatccatcagccgcggcatgaagtagatctcgccaatgtcggtcatggccagggtgaaggtacgctcgctggtcagcggatcgaagctttcatggtgctgtagggcgttgcgcagtgcgtgcatggccgaagtgacgggctcggccagatgcgcggcatagggtgtgggttccattccctgatgtgtgcgcacgaagagtgggtcctgtagcgaggtgcgcaggcgtttcagcgcattgctcacggcaggctgggtcaggcccaggttctccgcagtgatagagacgcgtctgtcgaccagcaactggttgaacaccaccagcaggtttaaatccaggtcacgcagttccatggggcctcgcttgggttattgctggtgcccggccgggcgcaatattcatgttgatgatttattatatatcgagtggtgtatttatcaatattgtttgctccgttatcgttattaacaagtcatcaataaagccatcacgagtaccatagtactagagcaattcggagagatgccggagcggctgaacggaccggtctctaaaaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactacagatccttagcgaaagctaaggattttttttaagcttactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtactagtttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctggtttccggcaccagaagcggtgccggaaagctggctggagtaataactgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K228851_sequence/1
type: Sequence
persistent identity: BBa K228851 sequence
display id: BBa_K228851_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcaatccgtaaacaggtcaaacatcagttgccgcaaccaaatattggctaggtccttgtggtacttcgcatgccagaacatgttgatggctatttcaggcaagacgactgggtgcggcaaggcgcttaggccgaagggctccacgcagcagtcggctaaacgtatcggcacagtggcgagcagatcggtgcgctggaggatgtggccaacggcggcgaagtgcggcacttccagacggatgtcgcgccggatgccgacccgtgtcatgtacgtgtccacctcgccgtggccggtgccagcggcgatgacacgcacgtggccgtaggaacagaagcgctccagagtcaggggttcgcgggtgactggatggtccttgcgacataggcacacgtagtgattctggagcagccggcgctgaaagaagccagtttgcagattgggaagcaggcccacggccaagtccacggttccgttctgcaaggcctgcatcaggctcatcgaactgtcgcgcaccgtactgatcacgcaattgggggcctggtgagccagcacatccatcagccgcggcatgaagtagatctcgccaatgtcggtcatggccagggtgaaggtacgctcgctggtcagcggatcgaagctttcatggtgctgtagggcgttgcgcagtgcgtgcatggccgaagtgacgggctcggccagatgcgcggcatagggtgtgggttccattccctgatgtgtgcgcacgaagagtgggtcctgtagcgaggtgcgcaggcgtttcagcgcattgctcacggcaggctgggtcaggcccaggttctccgcagtgatagagacgcgtctgtcgaccagcaactggttgaacaccaccagcaggtttaaatccaggtcacgcagttccatggggcctcgcttgggttattgctggtgcccggccgggcgcaatattcatgttgatgatttattatatatcgagtggtgtatttatcaatattgtttgctccgttatcgttattaacaagtcatcaataaagccatcacgagtaccatagtactagagcaattcggagagatgccggagcggctgaacggaccggtctctaaaaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactacagatccttagcgaaagctaaggattttttttaagcttactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K228867_sequence/1
type: Sequence
persistent identity: BBa K228867 sequence
display id: BBa_K228867_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagtttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctggtttccggcaccagaagcggtgccggaaagctggctggagtaataactgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatct…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K228001_sequence/1
type: Sequence
persistent identity: BBa K228001 sequence
display id: BBa_K228001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caattcggagagatgccggagcggctgaacggaccggtctctaaaaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactacagatccttagcgaaagctaaggattttttttaagct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K228004_sequence/1
type: Sequence
persistent identity: BBa K228004 sequence
display id: BBa_K228004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcaatccgtaaacaggtcaaacatcagttgccgcaaccaaatattggctaggtccttgtggtacttcgcatgccagaacatgttgatggctatttcaggcaagacgactgggtgcggcaaggcgcttaggccgaagggctccacgcagcagtcggctaaacgtatcggcacagtggcgagcagatcggtgcgctggaggatgtggccaacggcggcgaagtgcggcacttccagacggatgtcgcgccggatgccgacccgtgtcatgtacgtgtccacctcgccgtggccggtgccagcggcgatgacacgcacgtggccgtaggaacagaagcgctccagagtcaggggttcgcgggtgactggatggtccttgcgacataggcacacgtagtgattctggagcagccggcgctgaaagaagccagtttgcagattgggaagcaggcccacggccaagtccacggttccgttctgcaaggcctgcatcaggctcatcgaactgtcgcgcaccgtactgatcacgcaattgggggcctggtgagccagcacatccatcagccgcggcatgaagtagatctcgccaatgtcggtcatggccagggtgaaggtacgctcgctggtcagcggatcgaagctttcatggtgctgtagggcgttgcgcagtgcgtgcatggccgaagtgacgggctcggccagatgcgcggcatagggtgtgggttccattccctgatgtgtgcgcacgaagagtgggtcctgtagcgaggtgcgcaggcgtttcagcgcattgctcacggcaggctgggtcaggcccaggttctccgcagtgatagagacgcgtctgtcgaccagcaactggttgaacaccaccagcaggtttaaatccaggtcacgcagttccatggggcctcgcttgggttattgctggtgcccggccgggcgcaatattcatgttgatgatttattatatatcgagtggtgtatttatcaatattgtttgctccgttatcgttattaacaagtcatcaataaagccatcacgagtaccatag
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z