LOCUS BBa_K228877 5455 bp DNA linear 01-Nov-2024 DEFINITION AND GATE AraC+RBS(B0033)+T7ptag+Sal+SupD ACCESSION BBa_K228877 VERSION BBa_K228877.1 FEATURES Location/Qualifiers CDS 1..903 /label=NahR promoter 904..993 /label=Pr promoter promoter 982..1050 /label=pSal promoter 1..1052 /label=BBa_K228004 misc_feature 1061..1196 /label=BBa_K228001 stem_loop 1216..1259 /label=stem_loop misc_feature 1205..1284 /label=BBa_B0010 terminator 1205..1284 /label=BBa_B0010 stem_loop 1300..1319 /label=T7 TE misc_feature 1326..1326 /label=stop polyA_site 1320..1333 /label=polya misc_feature 1293..1333 /label=BBa_B0012 terminator 1293..1333 /label=BBa_B0012 misc_feature 1..1333 /label=BBa_K228851 stem_loop 2365..2408 /label=stem_loop misc_feature 2354..2433 /label=BBa_B0010 terminator 2354..2433 /label=BBa_B0010 stem_loop 2449..2468 /label=T7 TE misc_feature 2475..2475 /label=stop polyA_site 2469..2482 /label=polya misc_feature 2442..2482 /label=BBa_B0012 terminator 2442..2482 /label=BBa_B0012 promoter 2491..2620 /label=BBa_I13453 misc_feature 1342..1379 /label=P(Cat) promoter 1345..1350 /label=-35 promoter 1368..1373 /label=-10 misc_feature 1342..1379 /label=BBa_I14033 misc_feature 1388..1399 /label=BBa_B0034 misc_feature 1392..1395 /label=conserved misc_feature 1406..2320 /label=BBa_C0080 CDS 1406..2281 /label=araC misc_feature 2282..2314 /label=LVA misc_feature 2321..2345 /label=barcode misc_feature 2354..2433 /label=BBa_B0010 stem_loop 2365..2408 /label=stem_loop misc_feature 2442..2482 /label=BBa_B0012 stem_loop 2449..2468 /label=T7 TE polyA_site 2469..2482 /label=polya misc_feature 2475..2475 /label=stop misc_feature 1388..2482 /label=BBa_K228006 misc_feature 2491..2620 /label=BBa_I13453 misc_feature 1342..2482 /label=BBa_K228008 promoter 2491..2620 /label=BBa_I13453 promoter 1342..2620 /label=BBa_K228009 RBS 2629..2639 /label=RBS-4\Weaker RBS 2635..2638 /label=RBS misc_feature 2629..2639 /label=BBa_B0033 RBS 2629..2639 /label=BBa_B0033 CDS 2646..5318 /label=cds CDS 2646..5318 /label=BBa_K228000 stem_loop 5338..5381 /label=stem_loop misc_feature 5327..5406 /label=BBa_B0010 terminator 5327..5406 /label=BBa_B0010 stem_loop 5422..5441 /label=T7 TE misc_feature 5448..5448 /label=stop polyA_site 5442..5455 /label=polya misc_feature 5415..5455 /label=BBa_B0012 terminator 5415..5455 /label=BBa_B0012 terminator 5327..5455 /label=BBa_B0015 misc_feature 1342..5455 /label=BBa_K228868 ORIGIN 1 tcaatccgta aacaggtcaa acatcagttg ccgcaaccaa atattggcta ggtccttgtg 61 gtacttcgca tgccagaaca tgttgatggc tatttcaggc aagacgactg ggtgcggcaa 121 ggcgcttagg ccgaagggct ccacgcagca gtcggctaaa cgtatcggca cagtggcgag 181 cagatcggtg cgctggagga tgtggccaac ggcggcgaag tgcggcactt ccagacggat 241 gtcgcgccgg atgccgaccc gtgtcatgta cgtgtccacc tcgccgtggc cggtgccagc 301 ggcgatgaca cgcacgtggc cgtaggaaca gaagcgctcc agagtcaggg gttcgcgggt 361 gactggatgg tccttgcgac ataggcacac gtagtgattc tggagcagcc ggcgctgaaa 421 gaagccagtt tgcagattgg gaagcaggcc cacggccaag tccacggttc cgttctgcaa 481 ggcctgcatc aggctcatcg aactgtcgcg caccgtactg atcacgcaat tgggggcctg 541 gtgagccagc acatccatca gccgcggcat gaagtagatc tcgccaatgt cggtcatggc 601 cagggtgaag gtacgctcgc tggtcagcgg atcgaagctt tcatggtgct gtagggcgtt 661 gcgcagtgcg tgcatggccg aagtgacggg ctcggccaga tgcgcggcat agggtgtggg 721 ttccattccc tgatgtgtgc gcacgaagag tgggtcctgt agcgaggtgc gcaggcgttt 781 cagcgcattg ctcacggcag gctgggtcag gcccaggttc tccgcagtga tagagacgcg 841 tctgtcgacc agcaactggt tgaacaccac cagcaggttt aaatccaggt cacgcagttc 901 catggggcct cgcttgggtt attgctggtg cccggccggg cgcaatattc atgttgatga 961 tttattatat atcgagtggt gtatttatca atattgtttg ctccgttatc gttattaaca 1021 agtcatcaat aaagccatca cgagtaccat agtactagag caattcggag agatgccgga 1081 gcggctgaac ggaccggtct ctaaaaccgg agtaggggca actctaccgg gggttcaaat 1141 ccccctctct ccgccactac agatccttag cgaaagctaa ggattttttt taagcttact 1201 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 1261 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct 1321 ttctgcgttt atatactaga gtataaacgc agaaaggccc acccgaaggt gagccagtgt 1381 gactctagta gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtctttcg 1441 actgagcctt tcgttttatt tgatgcctgg ctctagtagt gatctacact agcactatca 1501 gtgttattaa gctactaaag cgtagttttc gtcgtttgca gctgacaact tgacggctac 1561 atcattcact ttttcttcac aaccggcacg gaactcgctc gggctggccc cggtgcattt 1621 tttaaatact cgcgagaaat agagttgatc gtcaaaacca acattgcgac cgacggtggc 1681 gataggcatc cgggtagtgc tcaaaagcag cttcgcctgg ctgatgcgtt ggtcctcgcg 1741 ccagcttaag acgctaatcc ctaactgctg gcggaaaaga tgtgacagac gcgacggcga 1801 caggcaaaca tgctgtgcga cgctggcgat atcaaaattg ctgtctgcca ggtgatcgct 1861 gatgtactga caagcctcgc gtacccgatt atccatcggt ggatggagcg actcgttaat 1921 cgcttccatg cgccgcagta acaattgctc aagcagattt atcgccagca gctccgaata 1981 gcgcccttcc ccttgcccgg cgttaatgat ttgcccaaac aggtcgctga aatgcggctg 2041 gtgcgcttca tccgggcgaa agaaacccgt attggcaaat attgacggcc agttaagcca 2101 ttcatgccag taggcgcgcg gacgaaagta aacccactgg tgataccatt cgcgagcctc 2161 cggatgacga ccgtagtgat gaatctctcc tggcgggaac agcaaaatat cacccggtcg 2221 gcagacaaat tctcgtccct gatttttcac caccccctga ccgcgaatgg tgagattgag 2281 aatataacct ttcattccca gcggtcggtc gataaaaaaa tcgagataac cattggcctc 2341 aatcggcgtt aaacccgcca ccagatgggc gttaaacgag tatcccggca gcaggggatc 2401 attttgcgct tcagccatct agtatttctc ctctttctct agtatgtttc attatggtga 2461 aagttggaac ctcttacgtg cctactagag acattgatta tttgcacggc gtcacacttt 2521 gctatgccat agcattttta tccataagat tagcggatcc tacctgacgc tttttatcgc 2581 aactctctac tgtttctcca taccgttttt ttgggctagc tactagagtc acacaggact 2641 actagatgac catgattacc gtgcactaga ataccattaa cattgctaag aacgacttct 2701 ctgacatcga actggctgct atcccgttca acactctggc tgaccattac ggtgagcgtt 2761 tagctcgcga acagttggcc cttgagcatg agtcttacga gatgggtgaa gcacgcttcc 2821 gcaagatgtt tgagcgtcaa cttaaagctg gtgaggttgc ggataacgct gccgccaagc 2881 ctctcatcac taccctactc cctaagatga ttgcacgcat caacgactgg tttgaggaag 2941 tgaaagctaa gcgcggcaag cgcccgacag ccttccagtt cctgtaggaa atcaagccgg 3001 aagccgtagc gtacatcacc attaagacca ctctggcttg cctaaccagt gctgacaata 3061 caaccgttca ggctgtagca agcgcaatcg gtcgggccat tgaggacgag gctcgcttcg 3121 gtcgtatccg tgaccttgaa gctaagcact tcaagaaaaa cgttgaggaa caactcaaca 3181 agcgcgtagg gcacgtctac aagaaagcat ttatgcaagt tgtcgaggct gacatgctct 3241 ctaagggtct actcggtggc gaggcgtggt cttcgtggca taaggaagac tctattcatg 3301 taggagtacg ctgcatcgag atgctcattg agtcaaccgg aatggttagc ttacaccgcc 3361 aaaatgctgg cgtagtaggt caagactctg agactatcga actcgcacct gaatacgctg 3421 aggctatcgc aacccgtgca ggtgcgctgg ctggcatctc tccgatgttc caaccttgcg 3481 tagttcctcc taagccgtgg actggcatta ctggtggtgg ctattgggct aacggtcgtc 3541 gtcctctggc gctggtgcgt actcacagta agaaagcact gatgcgctac gaagacgttt 3601 acatgcctga ggtgtacaaa gcgattaaca ttgcgcaaaa caccgcatgg aaaatcaaca 3661 agaaagtcct agcggtcgcc aacgtaatca ccaagtggaa gcattgtccg gtcgaggaca 3721 tccctgcgat tgagcgtgaa gaactcccga tgaaaccgga agacatcgac atgaatcctg 3781 aggctctcac cgcgtggaaa cgtgctgccg ctgctgtgta ccgcaaggac aaggctcgca 3841 agtctcgccg tatcagcctt gagttcatgc ttgagcaagc caataagttt gctaaccata 3901 aggccatctg gttcccttac aacatggact ggcgcggtcg tgtttacgct gtgtcaatgt 3961 tcaacccgca aggtaacgat atgaccaaag gactgcttac gctggcgaaa ggtaaaccaa 4021 tcggtaagga aggttactac tggctgaaaa tccacggtgc aaactgtgcg ggtgtcgata 4081 aggttccgtt ccctgagcgc atcaagttca ttgaggaaaa ccacgagaac atcatggctt 4141 gcgctaagtc tccactggag aacacttggt gggctgagca agattctccg ttctgcttcc 4201 ttgcgttctg ctttgagtac gctggggtac agcaccacgg cctgagctat aactgctccc 4261 ttccgctggc gtttgacggg tcttgctctg gcatccagca cttctccgcg atgctccgag 4321 atgaggtagg tggtcgcgcg gttaacttgc ttcctagtga aaccgttcag gacatctacg 4381 ggattgttgc taagaaagtc aacgagattc tacaagcaga cgcaatcaat gggaccgata 4441 acgaagtagt taccgtgacc gatgagaaca ctggtgaaat ctctgagaaa gtcaagctgg 4501 gcactaaggc actggctggt caatggctgg cttacggtgt tactcgcagt gtgactaagc 4561 gttcagtcat gacgctggct tacgggtcca aagagttcgg cttccgtcaa caagtgctgg 4621 aagataccat tcagccagct attgattccg gcaagggtct gatgttcact cagccgaatc 4681 aggctgctgg atacatggct aagctgattt gggaatctgt gagcgtgacg gtggtagctg 4741 cggttgaagc aatgaactgg cttaagtctg ctgctaagct gctggctgct gaggtcaaag 4801 ataagaagac tggagagatt cttcgcaagc gttgcgctgt gcattgggta actcctgatg 4861 gtttccctgt gtggcaggaa tacaagaagc ctattcagac gcgcttgaac ctgatgttcc 4921 tcggtcagtt ccgcttacag cctaccatta acaccaacaa agatagcgag attgatgcac 4981 acaaacagga gtctggtatc gctcctaact ttgtacacag ccaagacggt agccaccttc 5041 gtaagactgt agtgtgggca cacgagaagt acggaatcga atcttttgca ctgattcacg 5101 actccttcgg taccattccg gctgacgctg cgaacctgtt caaagcagtg cgcgaaacta 5161 tggttgacac atatgagtct tgtgatgtac tggctgattt ctacgaccag ttcgctgacc 5221 agttgcacga gtctcaattg gacaaaatgc cagcacttcc ggctaaaggt aacttgaacc 5281 tccgtgacat cttagagtcg gacttcgcgt tcgcataata ctagagccag gcatcaaata 5341 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac 5401 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata //