BBa_K228877
type: ComponentDefinition
persistent identity: BBa K228877
display id: BBa_K228877
version: 1
was generated by: iGEM to SBOL conversion
description: AND GATE AraC+RBS(B0033)+T7ptag+Sal+SupD
created: 2009-09-21T11:00:00Z
modified: 2015-05-08T01:11:35Z
mutable provenance: It is made up of standard subparts from the partregistry.
owned by: james
owned by: myers
mutable description: {{PKU_Beijing2009_AND_GATE_FAMILY}} This is an AND GATE in Ecoli. Use salicylate to induce the transcription of SupD and use arabinose to induce the transcription of T7ptag. If the SupD tRNA exists, T7ptag can be translated into functional T7 polymerase, and otherwise the translation of T7ptag will come to stop because of some amber stop codon mutations in the T7ptag. The T7 polymerase will activate a T7 promoter. This gate is only one of nine gates(K228870-K228878), which are distinguished by the RBS of T7ptag. For more information: refer to K228000(T7ptag) and K228001(SupD) referrence: Anderson JC, Voigt CA, Arkin AP (2007) Environmental signal integration by a modular AND gate. Mol Syst Biol 3: 133
discontinued: false
dominant: false
experience: experience/Issues
group u list: _353_
m user id: 0
owner id: 4411
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
star: false
creator: Haoqian Zhang
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K228868
component: BBa_K228851
sequence annotation: BBa_K228851
sequence annotation: BBa_K228868
sequence: https://synbiohub.org/public/igem/BBa_K228877_sequence/1

BBa_K228868
type: Component
persistent identity: component2286866
display id: component2286866
version: 1
top level: BBa_K228877
definition: BBa_K228868
access: public

BBa_K228851
type: Component
persistent identity: component2286832
display id: component2286832
version: 1
top level: BBa_K228877
definition: BBa_K228851
access: public

BBa_K228851
type: SequenceAnnotation
persistent identity: annotation2286832
display id: annotation2286832
version: 1
top level: BBa_K228877
location: https://synbiohub.org/public/igem/BBa_K228877/annotation2286832/range2286832/1
component: BBa_K228851

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type: Range
persistent identity: range2286832
display id: range2286832
version: 1
top level: BBa_K228877
direction: direction/reverse
start: 1
end: 1333
orientation: inline

BBa_K228868
type: SequenceAnnotation
persistent identity: annotation2286866
display id: annotation2286866
version: 1
top level: BBa_K228877
location: https://synbiohub.org/public/igem/BBa_K228877/annotation2286866/range2286866/1
component: BBa_K228868

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type: Range
persistent identity: range2286866
display id: range2286866
version: 1
top level: BBa_K228877
direction: direction/reverse
start: 1342
end: 5455
orientation: inline

BBa_K228851
type: ComponentDefinition
persistent identity: BBa K228851
display id: BBa_K228851
version: 1
was generated by: iGEM to SBOL conversion
description: Sal+supD+terminator
created: 2009-09-20T11:00:00Z
modified: 2015-05-08T01:11:34Z
owned by: james
owned by: myers
mutable description: This is a salicylate inducible part and can produce supD, which is a tRNA coding gene and it can be well terminated by the terminator BBa_B0015.
discontinued: false
dominant: false
experience: experience/Works
group u list: _353_
m user id: 0
owner id: 5502
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
star: false
creator: He Siheng
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K228004
component: BBa_B0010
component: BBa_K228001
component: BBa_B0012
sequence annotation: BBa_K228004
sequence annotation: BBa_K228001
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K228851_sequence/1

BBa_K228004
type: Component
persistent identity: component2023158
display id: component2023158
version: 1
top level: BBa_K228851
definition: BBa_K228004
access: public

BBa_B0010
type: Component
persistent identity: component2023160
display id: component2023160
version: 1
top level: BBa_K228851
definition: BBa_B0010
access: public

BBa_K228001
type: Component
persistent identity: component2023159
display id: component2023159
version: 1
top level: BBa_K228851
definition: BBa_K228001
access: public

BBa_B0012
type: Component
persistent identity: component2023162
display id: component2023162
version: 1
top level: BBa_K228851
definition: BBa_B0012
access: public

BBa_K228004
type: SequenceAnnotation
persistent identity: annotation2023158
display id: annotation2023158
version: 1
top level: BBa_K228851
location: https://synbiohub.org/public/igem/BBa_K228851/annotation2023158/range2023158/1
component: BBa_K228004

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type: Range
persistent identity: range2023158
display id: range2023158
version: 1
top level: BBa_K228851
direction: direction/reverse
start: 1
end: 1052
orientation: inline

BBa_K228001
type: SequenceAnnotation
persistent identity: annotation2023159
display id: annotation2023159
version: 1
top level: BBa_K228851
location: https://synbiohub.org/public/igem/BBa_K228851/annotation2023159/range2023159/1
component: BBa_K228001

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type: Range
persistent identity: range2023159
display id: range2023159
version: 1
top level: BBa_K228851
direction: direction/reverse
start: 1061
end: 1196
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2023160
display id: annotation2023160
version: 1
top level: BBa_K228851
location: https://synbiohub.org/public/igem/BBa_K228851/annotation2023160/range2023160/1
component: BBa_B0010

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type: Range
persistent identity: range2023160
display id: range2023160
version: 1
top level: BBa_K228851
direction: direction/forward
start: 1205
end: 1284
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2023162
display id: annotation2023162
version: 1
top level: BBa_K228851
location: https://synbiohub.org/public/igem/BBa_K228851/annotation2023162/range2023162/1
component: BBa_B0012

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type: Range
persistent identity: range2023162
display id: range2023162
version: 1
top level: BBa_K228851
direction: direction/forward
start: 1293
end: 1333
orientation: inline

BBa_K228868
type: ComponentDefinition
persistent identity: BBa K228868
display id: BBa_K228868
version: 1
was generated by: iGEM to SBOL conversion
description: AraC(K228009) + RBS(B0033) + T7ptag(K228000) + terminator(B0015)
created: 2009-09-21T11:00:00Z
modified: 2015-05-08T01:11:34Z
mutable provenance: from 2009 part registry distribution & Peking University 2009 igem team
owned by: james
owned by: myers
mutable description: T7 polymerase with an amber mutation coding gene is constructed after AraC protein(reversed sequence) and Pbad promoter which would be triggered by Arabinose. T7 polymerase can work well in the exsitence of SupD tRNA. Besides, the expression of the T7 polymerase can be regulated by RBS.
discontinued: false
dominant: false
group u list: _353_
m user id: 0
owner id: 4411
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: when cut this part for insert and ligate it into a vetor, the insert and vector may have the similar sizes which may disturb the ligation.
star: false
creator: Haoqian Zhang
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0012
component: BBa_K228000
component: BBa_B0033
component: BBa_B0015
component: BBa_B0010
component: BBa_K228009
sequence annotation: BBa_K228009
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_B0033
sequence annotation: BBa_K228000
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K228868_sequence/1

BBa_B0012
type: Component
persistent identity: component2262708
display id: component2262708
version: 1
top level: BBa_K228868
definition: BBa_B0012
access: public

BBa_K228000
type: Component
persistent identity: component2262719
display id: component2262719
version: 1
top level: BBa_K228868
definition: BBa_K228000
access: public

BBa_B0033
type: Component
persistent identity: component2262716
display id: component2262716
version: 1
top level: BBa_K228868
definition: BBa_B0033
access: public

BBa_B0015
type: Component
persistent identity: component2262726
display id: component2262726
version: 1
top level: BBa_K228868
definition: BBa_B0015
access: public

BBa_B0010
type: Component
persistent identity: component2262706
display id: component2262706
version: 1
top level: BBa_K228868
definition: BBa_B0010
access: public

BBa_K228009
type: Component
persistent identity: component2262714
display id: component2262714
version: 1
top level: BBa_K228868
definition: BBa_K228009
access: public

BBa_K228009
type: SequenceAnnotation
persistent identity: annotation2262714
display id: annotation2262714
version: 1
top level: BBa_K228868
location: https://synbiohub.org/public/igem/BBa_K228868/annotation2262714/range2262714/1
component: BBa_K228009

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type: Range
persistent identity: range2262714
display id: range2262714
version: 1
top level: BBa_K228868
direction: direction/reverse
start: 1
end: 1279
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2262706
display id: annotation2262706
version: 1
top level: BBa_K228868
location: https://synbiohub.org/public/igem/BBa_K228868/annotation2262706/range2262706/1
component: BBa_B0010

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type: Range
persistent identity: range2262706
display id: range2262706
version: 1
top level: BBa_K228868
direction: direction/forward
start: 1013
end: 1092
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2262708
display id: annotation2262708
version: 1
top level: BBa_K228868
location: https://synbiohub.org/public/igem/BBa_K228868/annotation2262708/range2262708/1
component: BBa_B0012

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type: Range
persistent identity: range2262708
display id: range2262708
version: 1
top level: BBa_K228868
direction: direction/forward
start: 1101
end: 1141
orientation: inline

BBa_B0033
type: SequenceAnnotation
persistent identity: annotation2262716
display id: annotation2262716
version: 1
top level: BBa_K228868
location: https://synbiohub.org/public/igem/BBa_K228868/annotation2262716/range2262716/1
component: BBa_B0033

https://synbiohub.org/public/igem/BBa_K228868/annotation2262716/range2262716/1
type: Range
persistent identity: range2262716
display id: range2262716
version: 1
top level: BBa_K228868
direction: direction/forward
start: 1288
end: 1298
orientation: inline

BBa_K228000
type: SequenceAnnotation
persistent identity: annotation2262719
display id: annotation2262719
version: 1
top level: BBa_K228868
location: https://synbiohub.org/public/igem/BBa_K228868/annotation2262719/range2262719/1
component: BBa_K228000

https://synbiohub.org/public/igem/BBa_K228868/annotation2262719/range2262719/1
type: Range
persistent identity: range2262719
display id: range2262719
version: 1
top level: BBa_K228868
direction: direction/reverse
start: 1305
end: 3977
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2262726
display id: annotation2262726
version: 1
top level: BBa_K228868
location: https://synbiohub.org/public/igem/BBa_K228868/annotation2262726/range2262726/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K228868/annotation2262726/range2262726/1
type: Range
persistent identity: range2262726
display id: range2262726
version: 1
top level: BBa_K228868
direction: direction/reverse
start: 3986
end: 4114
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_B0033
type: ComponentDefinition
persistent identity: BBa B0033
display id: BBa_B0033
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.4 (weaker) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weaker RBS based on Ron Weiss thesis. Strengths relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-3" in figure 4-14 of thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: RBS-4\Weaker
sequence annotation: BBa_B0033
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0033_sequence/1

RBS-4\Weaker
type: SequenceAnnotation
persistent identity: annotation1713
display id: annotation1713
version: 1
top level: BBa_B0033
location: https://synbiohub.org/public/igem/BBa_B0033/annotation1713/range1713/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0033/annotation1713/range1713/1
type: Range
persistent identity: range1713
display id: range1713
version: 1
top level: BBa_B0033
direction: direction/forward
start: 1
end: 11
orientation: inline

BBa_B0033
type: SequenceAnnotation
persistent identity: annotation7028
display id: annotation7028
version: 1
top level: BBa_B0033
location: https://synbiohub.org/public/igem/BBa_B0033/annotation7028/range7028/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0033/annotation7028/range7028/1
type: Range
persistent identity: range7028
display id: range7028
version: 1
top level: BBa_B0033
direction: direction/forward
start: 1
end: 11
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1714
display id: annotation1714
version: 1
top level: BBa_B0033
location: https://synbiohub.org/public/igem/BBa_B0033/annotation1714/range1714/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0033/annotation1714/range1714/1
type: Range
persistent identity: range1714
display id: range1714
version: 1
top level: BBa_B0033
direction: direction/forward
start: 7
end: 10
orientation: inline

BBa_K228000
type: ComponentDefinition
persistent identity: BBa K228000
display id: BBa_K228000
version: 1
was generated by: iGEM to SBOL conversion
description: T7ptag(T7polymerase with amber mutation)
created: 2009-08-24T11:00:00Z
modified: 2015-05-08T01:11:32Z
mutable provenance: The T7ptag is standardlized from the plasmid kindly provided by Voigt Lab.
owned by: james
owned by: myers
mutable description: T7ptag is a coding sequence of T7 polymerase with 2 Amber mutations. The transcription of T7ptag gene can only lead to the generation of its mRNA, further translation into T7 RNA polymerase is blocked because of the amber mutation. Only in the presence of the Amber mutation suppressor BBa_K228001 SupD-tRNA, it can be translated into functional T7 polymerase. T7ptag is a component of the AND Gate constructed by the Voigt Lab(UCSF)
discontinued: false
dominant: false
experience: experience/Works
group u list: _353_
m user id: 0
owner id: 4253
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
star: false
creator: Lin Min
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: cds
sequence: https://synbiohub.org/public/igem/BBa_K228000_sequence/1

cds
type: SequenceAnnotation
persistent identity: annotation2017714
display id: annotation2017714
version: 1
top level: BBa_K228000
location: https://synbiohub.org/public/igem/BBa_K228000/annotation2017714/range2017714/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K228000/annotation2017714/range2017714/1
type: Range
persistent identity: range2017714
display id: range2017714
version: 1
top level: BBa_K228000
direction: direction/forward
start: 1
end: 2673
orientation: inline

BBa_K228001
type: ComponentDefinition
persistent identity: BBa K228001
display id: BBa_K228001
version: 1
was generated by: iGEM to SBOL conversion
description: SupD-tRNA
created: 2009-08-24T11:00:00Z
modified: 2015-05-08T01:11:32Z
mutable provenance: SupD-tRNA again is from Voigt Lab(UCSF), We standardlize this part by PCR strategy.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The SupD-tRNA part functions as a tRNA, it suppresses the TAG amber stop codon and translate it into a Ser. There is no need to assembly SupD-tRNA with a rbs, since it is not translated. This part, together with the T7ptag part, make an AND Gate, that is, in the presence of both the 2 parts, T7 polymerase is tranlated into a functional polymerase that transcrips a downstream element constructed after a T7 promoter. Which is the output of the AND Gate.
discontinued: false
dominant: false
experience: experience/Works
group u list: _353_
m user id: 0
owner id: 4253
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: There is a PstI site in the original sequence, but luckily it is near the 3' end of this part, so we designed a mutation in our primer and mutated the PstI site.
star: false
creator: Lin Min
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence: https://synbiohub.org/public/igem/BBa_K228001_sequence/1

BBa_K228009
type: ComponentDefinition
persistent identity: BBa K228009
display id: BBa_K228009
version: 1
was generated by: iGEM to SBOL conversion
description: AraC protein(reversed sequence) and Pbad promoter
created: 2009-09-08T11:00:00Z
modified: 2015-05-08T01:11:32Z
owned by: james
owned by: myers
mutable description: This part contains AraC protein generator(BBa_K228008) and Pbad promoter(BBa_I13453). The expression of any coding sequence following the Pbad promoter is repressed constitutively by AraC protein and induced by L-arabinose.
discontinued: false
dominant: false
experience: experience/Works
group u list: _353_
m user id: 0
owner id: 4254
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: The maximum concentration of L-arabinose we tested is 10^-4 M.
star: false
creator: Gao Rencheng
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
component: BBa_K228008
component: BBa_I13453
sequence annotation: BBa_K228008
sequence annotation: BBa_I13453
sequence: https://synbiohub.org/public/igem/BBa_K228009_sequence/1

BBa_K228008
type: Component
persistent identity: component2244233
display id: component2244233
version: 1
top level: BBa_K228009
definition: BBa_K228008
access: public

BBa_I13453
type: Component
persistent identity: component2244232
display id: component2244232
version: 1
top level: BBa_K228009
definition: BBa_I13453
access: public

BBa_I13453
type: Component
persistent identity: component2244234
display id: component2244234
version: 1
top level: BBa_K228009
definition: BBa_I13453
access: public

BBa_K228008
type: SequenceAnnotation
persistent identity: annotation2244233
display id: annotation2244233
version: 1
top level: BBa_K228009
location: https://synbiohub.org/public/igem/BBa_K228009/annotation2244233/range2244233/1
component: BBa_K228008

https://synbiohub.org/public/igem/BBa_K228009/annotation2244233/range2244233/1
type: Range
persistent identity: range2244233
display id: range2244233
version: 1
top level: BBa_K228009
direction: direction/reverse
start: 1
end: 1141
orientation: inline

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation2244232
display id: annotation2244232
version: 1
top level: BBa_K228009
location: https://synbiohub.org/public/igem/BBa_K228009/annotation2244232/range2244232/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_K228009/annotation2244232/range2244232/1
type: Range
persistent identity: range2244232
display id: range2244232
version: 1
top level: BBa_K228009
direction: direction/reverse
start: 1150
end: 1279
orientation: inline

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation2244234
display id: annotation2244234
version: 1
top level: BBa_K228009
location: https://synbiohub.org/public/igem/BBa_K228009/annotation2244234/range2244234/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_K228009/annotation2244234/range2244234/1
type: Range
persistent identity: range2244234
display id: range2244234
version: 1
top level: BBa_K228009
direction: direction/reverse
start: 1150
end: 1279
orientation: inline

BBa_K228004
type: ComponentDefinition
persistent identity: BBa K228004
display id: BBa_K228004
version: 1
was generated by: iGEM to SBOL conversion
description: NahR( reverse) - salicylate promoter
created: 2009-09-17T11:00:00Z
modified: 2015-05-08T01:11:32Z
mutable provenance: It is constrcuted by PCR from the plasmid kindly provided by Voigt Lab in UCSF.
owned by: james
owned by: myers
mutable description: This is a salicylate inducible promoter part. The activator protein is right upstream but in a opposite direction of the regulated promoter. In the presence of salicylate, the promoter can be activated.
discontinued: false
dominant: false
experience: experience/Works
group u list: _353_
m user id: 0
owner id: 4253
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: There is no termoinator downstream of the NahR coding sequence, but it has no effect on parts that are in the same direction as the pSal promoter. However, if coding gene is placed upstream of this part and in the same direction as NahR, it may cause unwanted expression.
star: false
creator: Lin Min
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: NahR
sequence annotation: Pr promoter
sequence annotation: pSal
sequence: https://synbiohub.org/public/igem/BBa_K228004_sequence/1

NahR
type: SequenceAnnotation
persistent identity: annotation2075285
display id: annotation2075285
version: 1
top level: BBa_K228004
location: https://synbiohub.org/public/igem/BBa_K228004/annotation2075285/range2075285/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K228004/annotation2075285/range2075285/1
type: Range
persistent identity: range2075285
display id: range2075285
version: 1
top level: BBa_K228004
direction: direction/reverse
start: 1
end: 903
orientation: inline

Pr promoter
type: SequenceAnnotation
persistent identity: annotation2075286
display id: annotation2075286
version: 1
top level: BBa_K228004
location: https://synbiohub.org/public/igem/BBa_K228004/annotation2075286/range2075286/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K228004/annotation2075286/range2075286/1
type: Range
persistent identity: range2075286
display id: range2075286
version: 1
top level: BBa_K228004
direction: direction/reverse
start: 904
end: 993
orientation: inline

pSal
type: SequenceAnnotation
persistent identity: annotation2075287
display id: annotation2075287
version: 1
top level: BBa_K228004
location: https://synbiohub.org/public/igem/BBa_K228004/annotation2075287/range2075287/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K228004/annotation2075287/range2075287/1
type: Range
persistent identity: range2075287
display id: range2075287
version: 1
top level: BBa_K228004
direction: direction/forward
start: 982
end: 1050
orientation: inline

BBa_I13453
type: ComponentDefinition
persistent identity: BBa I13453
display id: BBa_I13453
version: 1
was generated by: iGEM to SBOL conversion
description: Pbad promoter
created: 2005-05-24T11:00:00Z
modified: 2015-08-31T04:07:34Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: PBad promoter from I0500 without AraC.
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jkm
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I13453_sequence/1

BBa_K228008
type: ComponentDefinition
persistent identity: BBa K228008
display id: BBa_K228008
version: 1
was generated by: iGEM to SBOL conversion
description: AraC regulatory protein
created: 2009-09-08T11:00:00Z
modified: 2015-05-08T01:11:32Z
owned by: james
owned by: myers
mutable description: This part could be connected to a protein coding sequence so that the expression could be induced by L-arabinose
discontinued: false
dominant: false
experience: experience/Works
group u list: _353_
m user id: 0
owner id: 4254
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
star: false
creator: Gao Rencheng
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: BBa_I14033
sequence annotation: P(Cat)
sequence annotation: -35
sequence annotation: -10
sequence annotation: BBa_K228006
sequence annotation: BBa_B0034
sequence annotation: conserved
sequence annotation: araC
sequence annotation: BBa_C0080
sequence annotation: LVA
sequence annotation: barcode
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence annotation: BBa_I13453
sequence: https://synbiohub.org/public/igem/BBa_K228008_sequence/1

BBa_I14033
type: SequenceAnnotation
persistent identity: annotation2020002
display id: annotation2020002
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020002/range2020002/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K228008/annotation2020002/range2020002/1
type: Range
persistent identity: range2020002
display id: range2020002
version: 1
top level: BBa_K228008
direction: direction/reverse
start: 1
end: 38
orientation: inline

P(Cat)
type: SequenceAnnotation
persistent identity: annotation2019999
display id: annotation2019999
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2019999/range2019999/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_K228008/annotation2019999/range2019999/1
type: Range
persistent identity: range2019999
display id: range2019999
version: 1
top level: BBa_K228008
direction: direction/forward
start: 1
end: 38
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2020000
display id: annotation2020000
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020000/range2020000/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K228008/annotation2020000/range2020000/1
type: Range
persistent identity: range2020000
display id: range2020000
version: 1
top level: BBa_K228008
direction: direction/forward
start: 4
end: 9
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2020001
display id: annotation2020001
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020001/range2020001/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K228008/annotation2020001/range2020001/1
type: Range
persistent identity: range2020001
display id: range2020001
version: 1
top level: BBa_K228008
direction: direction/forward
start: 27
end: 32
orientation: inline

BBa_K228006
type: SequenceAnnotation
persistent identity: annotation2020015
display id: annotation2020015
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020015/range2020015/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K228008/annotation2020015/range2020015/1
type: Range
persistent identity: range2020015
display id: range2020015
version: 1
top level: BBa_K228008
direction: direction/reverse
start: 47
end: 1141
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2020003
display id: annotation2020003
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020003/range2020003/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K228008/annotation2020003/range2020003/1
type: Range
persistent identity: range2020003
display id: range2020003
version: 1
top level: BBa_K228008
direction: direction/reverse
start: 47
end: 58
orientation: inline

conserved
type: SequenceAnnotation
persistent identity: annotation2020004
display id: annotation2020004
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020004/range2020004/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_K228008/annotation2020004/range2020004/1
type: Range
persistent identity: range2020004
display id: range2020004
version: 1
top level: BBa_K228008
direction: direction/forward
start: 51
end: 54
orientation: inline

araC
type: SequenceAnnotation
persistent identity: annotation2020006
display id: annotation2020006
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020006/range2020006/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K228008/annotation2020006/range2020006/1
type: Range
persistent identity: range2020006
display id: range2020006
version: 1
top level: BBa_K228008
direction: direction/forward
start: 65
end: 940
orientation: inline

BBa_C0080
type: SequenceAnnotation
persistent identity: annotation2020005
display id: annotation2020005
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020005/range2020005/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K228008/annotation2020005/range2020005/1
type: Range
persistent identity: range2020005
display id: range2020005
version: 1
top level: BBa_K228008
direction: direction/reverse
start: 65
end: 979
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation2020007
display id: annotation2020007
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020007/range2020007/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_K228008/annotation2020007/range2020007/1
type: Range
persistent identity: range2020007
display id: range2020007
version: 1
top level: BBa_K228008
direction: direction/forward
start: 941
end: 973
orientation: inline

barcode
type: SequenceAnnotation
persistent identity: annotation2020008
display id: annotation2020008
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020008/range2020008/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K228008/annotation2020008/range2020008/1
type: Range
persistent identity: range2020008
display id: range2020008
version: 1
top level: BBa_K228008
direction: direction/reverse
start: 980
end: 1004
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2020009
display id: annotation2020009
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020009/range2020009/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K228008/annotation2020009/range2020009/1
type: Range
persistent identity: range2020009
display id: range2020009
version: 1
top level: BBa_K228008
direction: direction/forward
start: 1013
end: 1092
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation2020010
display id: annotation2020010
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020010/range2020010/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_K228008/annotation2020010/range2020010/1
type: Range
persistent identity: range2020010
display id: range2020010
version: 1
top level: BBa_K228008
direction: direction/forward
start: 1024
end: 1067
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2020011
display id: annotation2020011
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020011/range2020011/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K228008/annotation2020011/range2020011/1
type: Range
persistent identity: range2020011
display id: range2020011
version: 1
top level: BBa_K228008
direction: direction/forward
start: 1101
end: 1141
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation2020012
display id: annotation2020012
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020012/range2020012/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_K228008/annotation2020012/range2020012/1
type: Range
persistent identity: range2020012
display id: range2020012
version: 1
top level: BBa_K228008
direction: direction/forward
start: 1108
end: 1127
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation2020013
display id: annotation2020013
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020013/range2020013/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_K228008/annotation2020013/range2020013/1
type: Range
persistent identity: range2020013
display id: range2020013
version: 1
top level: BBa_K228008
direction: direction/forward
start: 1128
end: 1141
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2020014
display id: annotation2020014
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020014/range2020014/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K228008/annotation2020014/range2020014/1
type: Range
persistent identity: range2020014
display id: range2020014
version: 1
top level: BBa_K228008
direction: direction/forward
start: 1134
end: 1134
orientation: inline

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation2020016
display id: annotation2020016
version: 1
top level: BBa_K228008
location: https://synbiohub.org/public/igem/BBa_K228008/annotation2020016/range2020016/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K228008/annotation2020016/range2020016/1
type: Range
persistent identity: range2020016
display id: range2020016
version: 1
top level: BBa_K228008
direction: direction/reverse
start: 1150
end: 1279
orientation: inline

https://synbiohub.org/public/igem/BBa_K228877_sequence/1
type: Sequence
persistent identity: BBa K228877 sequence
display id: BBa_K228877_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcaatccgtaaacaggtcaaacatcagttgccgcaaccaaatattggctaggtccttgtggtacttcgcatgccagaacatgttgatggctatttcaggcaagacgactgggtgcggcaaggcgcttaggccgaagggctccacgcagcagtcggctaaacgtatcggcacagtggcgagcagatcggtgcgctggaggatgtggccaacggcggcgaagtgcggcacttccagacggatgtcgcgccggatgccgacccgtgtcatgtacgtgtccacctcgccgtggccggtgccagcggcgatgacacgcacgtggccgtaggaacagaagcgctccagagtcaggggttcgcgggtgactggatggtccttgcgacataggcacacgtagtgattctggagcagccggcgctgaaagaagccagtttgcagattgggaagcaggcccacggccaagtccacggttccgttctgcaaggcctgcatcaggctcatcgaactgtcgcgcaccgtactgatcacgcaattgggggcctggtgagccagcacatccatcagccgcggcatgaagtagatctcgccaatgtcggtcatggccagggtgaaggtacgctcgctggtcagcggatcgaagctttcatggtgctgtagggcgttgcgcagtgcgtgcatggccgaagtgacgggctcggccagatgcgcggcatagggtgtgggttccattccctgatgtgtgcgcacgaagagtgggtcctgtagcgaggtgcgcaggcgtttcagcgcattgctcacggcaggctgggtcaggcccaggttctccgcagtgatagagacgcgtctgtcgaccagcaactggttgaacaccaccagcaggtttaaatccaggtcacgcagttccatggggcctcgcttgggttattgctggtgcccggccgggcgcaatattcatgttgatgatttattatatatcgagtggtgtatttatcaatattgtttgctccgttatcgttattaacaagtcatcaataaagccatcacgagtaccatagtactagagcaattcggagagatgccggagcggctgaacggaccggtctctaaaaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactacagatccttagcgaaagctaaggattttttttaagcttactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggctctagtagtgatctacactagcactatcagtgttattaagctactaaagcgtagttttcgtcgtttgcagctgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatactcgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtagtgctcaaaagcagcttcgcctggctgatgcgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaggcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccgg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K228851_sequence/1
type: Sequence
persistent identity: BBa K228851 sequence
display id: BBa_K228851_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcaatccgtaaacaggtcaaacatcagttgccgcaaccaaatattggctaggtccttgtggtacttcgcatgccagaacatgttgatggctatttcaggcaagacgactgggtgcggcaaggcgcttaggccgaagggctccacgcagcagtcggctaaacgtatcggcacagtggcgagcagatcggtgcgctggaggatgtggccaacggcggcgaagtgcggcacttccagacggatgtcgcgccggatgccgacccgtgtcatgtacgtgtccacctcgccgtggccggtgccagcggcgatgacacgcacgtggccgtaggaacagaagcgctccagagtcaggggttcgcgggtgactggatggtccttgcgacataggcacacgtagtgattctggagcagccggcgctgaaagaagccagtttgcagattgggaagcaggcccacggccaagtccacggttccgttctgcaaggcctgcatcaggctcatcgaactgtcgcgcaccgtactgatcacgcaattgggggcctggtgagccagcacatccatcagccgcggcatgaagtagatctcgccaatgtcggtcatggccagggtgaaggtacgctcgctggtcagcggatcgaagctttcatggtgctgtagggcgttgcgcagtgcgtgcatggccgaagtgacgggctcggccagatgcgcggcatagggtgtgggttccattccctgatgtgtgcgcacgaagagtgggtcctgtagcgaggtgcgcaggcgtttcagcgcattgctcacggcaggctgggtcaggcccaggttctccgcagtgatagagacgcgtctgtcgaccagcaactggttgaacaccaccagcaggtttaaatccaggtcacgcagttccatggggcctcgcttgggttattgctggtgcccggccgggcgcaatattcatgttgatgatttattatatatcgagtggtgtatttatcaatattgtttgctccgttatcgttattaacaagtcatcaataaagccatcacgagtaccatagtactagagcaattcggagagatgccggagcggctgaacggaccggtctctaaaaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactacagatccttagcgaaagctaaggattttttttaagcttactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K228868_sequence/1
type: Sequence
persistent identity: BBa K228868 sequence
display id: BBa_K228868_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggctctagtagtgatctacactagcactatcagtgttattaagctactaaagcgtagttttcgtcgtttgcagctgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatactcgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtagtgctcaaaagcagcttcgcctggctgatgcgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaggcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaacccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcagacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccattggcctcaatcggcgttaaacccgccaccagatgggcgttaaacgagtatcccggcagcaggggatcattttgcgcttcagccatctagtatttctcctctttctctagtatgtttcattatggtgaaagttggaacctcttacgtgcctactagagacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagtcacacaggactactagatgaccatgattaccgtgcactagaataccattaacattgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgtaggaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccgga…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0033_sequence/1
type: Sequence
persistent identity: BBa B0033 sequence
display id: BBa_B0033_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K228000_sequence/1
type: Sequence
persistent identity: BBa K228000 sequence
display id: BBa_K228000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaccatgattaccgtgcactagaataccattaacattgctaagaacgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgagcgtttagctcgcgaacagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctggtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactggtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgtaggaaatcaagccggaagccgtagcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaatcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgttgaggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaagggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgctgcatcgagatgctcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaactcgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgttccaaccttgcgtagttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctggcgctggtgcgtactcacagtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggtgtacaaagcgattaacattgcgcaaaacaccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgtccggtcgaggacatccctgcgattgagcgtgaagaactcccgatgaaaccggaagacatcgacatgaatcctgaggctctcaccgcgtggaaacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagttcatgcttgagcaagccaataagtttgctaaccataaggccatctggttcccttacaacatggactggcgcggtcgtgtttacgctgtgtcaatgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatcggtaaggaaggttactactggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgcatcaagttcattgaggaaaaccacgagaacatcatggcttgcgctaagtctccactggagaacacttggtgggctgagcaagattctccgttctgcttccttgcgttctgctttgagtacgctggggtacagcaccacggcctgagctataactgctcccttccgctggcgtttgacgggtcttgctctggcatccagcacttctccgcgatgctccgagatgaggtaggtggtcgcgcggttaacttgcttcctagtgaaaccgttcaggacatctacgggattgttgctaagaaagtcaacgagattctacaagcagacgcaatcaatgggaccgataacgaagtagttaccgtgaccgatgagaacactggtgaaatctctgagaaagtcaagctgggcactaaggcactggctggtcaatggctggcttacggtgttactcgcagtgtgactaagcgttcagtcatgacgctggcttacgggtccaaagagttcggcttccgtcaacaagtgctggaagataccattcagccagctattga…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K228001_sequence/1
type: Sequence
persistent identity: BBa K228001 sequence
display id: BBa_K228001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caattcggagagatgccggagcggctgaacggaccggtctctaaaaccggagtaggggcaactctaccgggggttcaaatccccctctctccgccactacagatccttagcgaaagctaaggattttttttaagct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K228009_sequence/1
type: Sequence
persistent identity: BBa K228009 sequence
display id: BBa_K228009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggctctagtagtgatctacactagcactatcagtgttattaagctactaaagcgtagttttcgtcgtttgcagctgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatactcgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtagtgctcaaaagcagcttcgcctggctgatgcgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaggcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaacccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcagacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccattggcctcaatcggcgttaaacccgccaccagatgggcgttaaacgagtatcccggcagcaggggatcattttgcgcttcagccatctagtatttctcctctttctctagtatgtttcattatggtgaaagttggaacctcttacgtgcctactagagacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K228004_sequence/1
type: Sequence
persistent identity: BBa K228004 sequence
display id: BBa_K228004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcaatccgtaaacaggtcaaacatcagttgccgcaaccaaatattggctaggtccttgtggtacttcgcatgccagaacatgttgatggctatttcaggcaagacgactgggtgcggcaaggcgcttaggccgaagggctccacgcagcagtcggctaaacgtatcggcacagtggcgagcagatcggtgcgctggaggatgtggccaacggcggcgaagtgcggcacttccagacggatgtcgcgccggatgccgacccgtgtcatgtacgtgtccacctcgccgtggccggtgccagcggcgatgacacgcacgtggccgtaggaacagaagcgctccagagtcaggggttcgcgggtgactggatggtccttgcgacataggcacacgtagtgattctggagcagccggcgctgaaagaagccagtttgcagattgggaagcaggcccacggccaagtccacggttccgttctgcaaggcctgcatcaggctcatcgaactgtcgcgcaccgtactgatcacgcaattgggggcctggtgagccagcacatccatcagccgcggcatgaagtagatctcgccaatgtcggtcatggccagggtgaaggtacgctcgctggtcagcggatcgaagctttcatggtgctgtagggcgttgcgcagtgcgtgcatggccgaagtgacgggctcggccagatgcgcggcatagggtgtgggttccattccctgatgtgtgcgcacgaagagtgggtcctgtagcgaggtgcgcaggcgtttcagcgcattgctcacggcaggctgggtcaggcccaggttctccgcagtgatagagacgcgtctgtcgaccagcaactggttgaacaccaccagcaggtttaaatccaggtcacgcagttccatggggcctcgcttgggttattgctggtgcccggccgggcgcaatattcatgttgatgatttattatatatcgagtggtgtatttatcaatattgtttgctccgttatcgttattaacaagtcatcaataaagccatcacgagtaccatag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I13453_sequence/1
type: Sequence
persistent identity: BBa I13453 sequence
display id: BBa_I13453_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K228008_sequence/1
type: Sequence
persistent identity: BBa K228008 sequence
display id: BBa_K228008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggctctagtagtgatctacactagcactatcagtgttattaagctactaaagcgtagttttcgtcgtttgcagctgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatactcgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtagtgctcaaaagcagcttcgcctggctgatgcgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaggcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaacccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcagacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccattggcctcaatcggcgttaaacccgccaccagatgggcgttaaacgagtatcccggcagcaggggatcattttgcgcttcagccatctagtatttctcctctttctctagtatgtttcattatggtgaaagttggaacctcttacgtgcc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z