LOCUS BBa_K228878 5458 bp DNA linear 22-Nov-2024 DEFINITION AND GATE AraC+RBS(B0031)+T7ptag+Sal+SupD ACCESSION BBa_K228878 VERSION BBa_K228878.1 FEATURES Location/Qualifiers CDS 1..903 /label=NahR promoter 904..993 /label=Pr promoter promoter 982..1050 /label=pSal promoter 1..1052 /label=BBa_K228004 misc_feature 1061..1196 /label=BBa_K228001 stem_loop 1216..1259 /label=stem_loop misc_feature 1205..1284 /label=BBa_B0010 terminator 1205..1284 /label=BBa_B0010 stem_loop 1300..1319 /label=T7 TE misc_feature 1326..1326 /label=stop polyA_site 1320..1333 /label=polya misc_feature 1293..1333 /label=BBa_B0012 terminator 1293..1333 /label=BBa_B0012 misc_feature 1..1333 /label=BBa_K228851 stem_loop 2365..2408 /label=stem_loop misc_feature 2354..2433 /label=BBa_B0010 terminator 2354..2433 /label=BBa_B0010 stem_loop 2449..2468 /label=T7 TE misc_feature 2475..2475 /label=stop polyA_site 2469..2482 /label=polya misc_feature 2442..2482 /label=BBa_B0012 terminator 2442..2482 /label=BBa_B0012 promoter 2491..2620 /label=BBa_I13453 misc_feature 1342..1379 /label=P(Cat) promoter 1345..1350 /label=-35 promoter 1368..1373 /label=-10 misc_feature 1342..1379 /label=BBa_I14033 misc_feature 1388..1399 /label=BBa_B0034 misc_feature 1392..1395 /label=conserved misc_feature 1406..2320 /label=BBa_C0080 CDS 1406..2281 /label=araC misc_feature 2282..2314 /label=LVA misc_feature 2321..2345 /label=barcode misc_feature 2354..2433 /label=BBa_B0010 stem_loop 2365..2408 /label=stem_loop misc_feature 2442..2482 /label=BBa_B0012 stem_loop 2449..2468 /label=T7 TE polyA_site 2469..2482 /label=polya misc_feature 2475..2475 /label=stop misc_feature 1388..2482 /label=BBa_K228006 misc_feature 2491..2620 /label=BBa_I13453 misc_feature 1342..2482 /label=BBa_K228008 promoter 2491..2620 /label=BBa_I13453 promoter 1342..2620 /label=BBa_K228009 misc_feature 2635..2638 /label=conserved RBS 2629..2642 /label=BBa_B0031 CDS 2649..5321 /label=cds CDS 2649..5321 /label=BBa_K228000 stem_loop 5341..5384 /label=stem_loop misc_feature 5330..5409 /label=BBa_B0010 terminator 5330..5409 /label=BBa_B0010 stem_loop 5425..5444 /label=T7 TE misc_feature 5451..5451 /label=stop polyA_site 5445..5458 /label=polya misc_feature 5418..5458 /label=BBa_B0012 terminator 5418..5458 /label=BBa_B0012 terminator 5330..5458 /label=BBa_B0015 misc_feature 1342..5458 /label=BBa_K228869 ORIGIN 1 tcaatccgta aacaggtcaa acatcagttg ccgcaaccaa atattggcta ggtccttgtg 61 gtacttcgca tgccagaaca tgttgatggc tatttcaggc aagacgactg ggtgcggcaa 121 ggcgcttagg ccgaagggct ccacgcagca gtcggctaaa cgtatcggca cagtggcgag 181 cagatcggtg cgctggagga tgtggccaac ggcggcgaag tgcggcactt ccagacggat 241 gtcgcgccgg atgccgaccc gtgtcatgta cgtgtccacc tcgccgtggc cggtgccagc 301 ggcgatgaca cgcacgtggc cgtaggaaca gaagcgctcc agagtcaggg gttcgcgggt 361 gactggatgg tccttgcgac ataggcacac gtagtgattc tggagcagcc ggcgctgaaa 421 gaagccagtt tgcagattgg gaagcaggcc cacggccaag tccacggttc cgttctgcaa 481 ggcctgcatc aggctcatcg aactgtcgcg caccgtactg atcacgcaat tgggggcctg 541 gtgagccagc acatccatca gccgcggcat gaagtagatc tcgccaatgt cggtcatggc 601 cagggtgaag gtacgctcgc tggtcagcgg atcgaagctt tcatggtgct gtagggcgtt 661 gcgcagtgcg tgcatggccg aagtgacggg ctcggccaga tgcgcggcat agggtgtggg 721 ttccattccc tgatgtgtgc gcacgaagag tgggtcctgt agcgaggtgc gcaggcgttt 781 cagcgcattg ctcacggcag gctgggtcag gcccaggttc tccgcagtga tagagacgcg 841 tctgtcgacc agcaactggt tgaacaccac cagcaggttt aaatccaggt cacgcagttc 901 catggggcct cgcttgggtt attgctggtg cccggccggg cgcaatattc atgttgatga 961 tttattatat atcgagtggt gtatttatca atattgtttg ctccgttatc gttattaaca 1021 agtcatcaat aaagccatca cgagtaccat agtactagag caattcggag agatgccgga 1081 gcggctgaac ggaccggtct ctaaaaccgg agtaggggca actctaccgg gggttcaaat 1141 ccccctctct ccgccactac agatccttag cgaaagctaa ggattttttt taagcttact 1201 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 1261 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct 1321 ttctgcgttt atatactaga gtataaacgc agaaaggccc acccgaaggt gagccagtgt 1381 gactctagta gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtctttcg 1441 actgagcctt tcgttttatt tgatgcctgg ctctagtagt gatctacact agcactatca 1501 gtgttattaa gctactaaag cgtagttttc gtcgtttgca gctgacaact tgacggctac 1561 atcattcact ttttcttcac aaccggcacg gaactcgctc gggctggccc cggtgcattt 1621 tttaaatact cgcgagaaat agagttgatc gtcaaaacca acattgcgac cgacggtggc 1681 gataggcatc cgggtagtgc tcaaaagcag cttcgcctgg ctgatgcgtt ggtcctcgcg 1741 ccagcttaag acgctaatcc ctaactgctg gcggaaaaga tgtgacagac gcgacggcga 1801 caggcaaaca tgctgtgcga cgctggcgat atcaaaattg ctgtctgcca ggtgatcgct 1861 gatgtactga caagcctcgc gtacccgatt atccatcggt ggatggagcg actcgttaat 1921 cgcttccatg cgccgcagta acaattgctc aagcagattt atcgccagca gctccgaata 1981 gcgcccttcc ccttgcccgg cgttaatgat ttgcccaaac aggtcgctga aatgcggctg 2041 gtgcgcttca tccgggcgaa agaaacccgt attggcaaat attgacggcc agttaagcca 2101 ttcatgccag taggcgcgcg gacgaaagta aacccactgg tgataccatt cgcgagcctc 2161 cggatgacga ccgtagtgat gaatctctcc tggcgggaac agcaaaatat cacccggtcg 2221 gcagacaaat tctcgtccct gatttttcac caccccctga ccgcgaatgg tgagattgag 2281 aatataacct ttcattccca gcggtcggtc gataaaaaaa tcgagataac cattggcctc 2341 aatcggcgtt aaacccgcca ccagatgggc gttaaacgag tatcccggca gcaggggatc 2401 attttgcgct tcagccatct agtatttctc ctctttctct agtatgtttc attatggtga 2461 aagttggaac ctcttacgtg cctactagag acattgatta tttgcacggc gtcacacttt 2521 gctatgccat agcattttta tccataagat tagcggatcc tacctgacgc tttttatcgc 2581 aactctctac tgtttctcca taccgttttt ttgggctagc tactagagtc acacaggaaa 2641 cctactagat gaccatgatt accgtgcact agaataccat taacattgct aagaacgact 2701 tctctgacat cgaactggct gctatcccgt tcaacactct ggctgaccat tacggtgagc 2761 gtttagctcg cgaacagttg gcccttgagc atgagtctta cgagatgggt gaagcacgct 2821 tccgcaagat gtttgagcgt caacttaaag ctggtgaggt tgcggataac gctgccgcca 2881 agcctctcat cactacccta ctccctaaga tgattgcacg catcaacgac tggtttgagg 2941 aagtgaaagc taagcgcggc aagcgcccga cagccttcca gttcctgtag gaaatcaagc 3001 cggaagccgt agcgtacatc accattaaga ccactctggc ttgcctaacc agtgctgaca 3061 atacaaccgt tcaggctgta gcaagcgcaa tcggtcgggc cattgaggac gaggctcgct 3121 tcggtcgtat ccgtgacctt gaagctaagc acttcaagaa aaacgttgag gaacaactca 3181 acaagcgcgt agggcacgtc tacaagaaag catttatgca agttgtcgag gctgacatgc 3241 tctctaaggg tctactcggt ggcgaggcgt ggtcttcgtg gcataaggaa gactctattc 3301 atgtaggagt acgctgcatc gagatgctca ttgagtcaac cggaatggtt agcttacacc 3361 gccaaaatgc tggcgtagta ggtcaagact ctgagactat cgaactcgca cctgaatacg 3421 ctgaggctat cgcaacccgt gcaggtgcgc tggctggcat ctctccgatg ttccaacctt 3481 gcgtagttcc tcctaagccg tggactggca ttactggtgg tggctattgg gctaacggtc 3541 gtcgtcctct ggcgctggtg cgtactcaca gtaagaaagc actgatgcgc tacgaagacg 3601 tttacatgcc tgaggtgtac aaagcgatta acattgcgca aaacaccgca tggaaaatca 3661 acaagaaagt cctagcggtc gccaacgtaa tcaccaagtg gaagcattgt ccggtcgagg 3721 acatccctgc gattgagcgt gaagaactcc cgatgaaacc ggaagacatc gacatgaatc 3781 ctgaggctct caccgcgtgg aaacgtgctg ccgctgctgt gtaccgcaag gacaaggctc 3841 gcaagtctcg ccgtatcagc cttgagttca tgcttgagca agccaataag tttgctaacc 3901 ataaggccat ctggttccct tacaacatgg actggcgcgg tcgtgtttac gctgtgtcaa 3961 tgttcaaccc gcaaggtaac gatatgacca aaggactgct tacgctggcg aaaggtaaac 4021 caatcggtaa ggaaggttac tactggctga aaatccacgg tgcaaactgt gcgggtgtcg 4081 ataaggttcc gttccctgag cgcatcaagt tcattgagga aaaccacgag aacatcatgg 4141 cttgcgctaa gtctccactg gagaacactt ggtgggctga gcaagattct ccgttctgct 4201 tccttgcgtt ctgctttgag tacgctgggg tacagcacca cggcctgagc tataactgct 4261 cccttccgct ggcgtttgac gggtcttgct ctggcatcca gcacttctcc gcgatgctcc 4321 gagatgaggt aggtggtcgc gcggttaact tgcttcctag tgaaaccgtt caggacatct 4381 acgggattgt tgctaagaaa gtcaacgaga ttctacaagc agacgcaatc aatgggaccg 4441 ataacgaagt agttaccgtg accgatgaga acactggtga aatctctgag aaagtcaagc 4501 tgggcactaa ggcactggct ggtcaatggc tggcttacgg tgttactcgc agtgtgacta 4561 agcgttcagt catgacgctg gcttacgggt ccaaagagtt cggcttccgt caacaagtgc 4621 tggaagatac cattcagcca gctattgatt ccggcaaggg tctgatgttc actcagccga 4681 atcaggctgc tggatacatg gctaagctga tttgggaatc tgtgagcgtg acggtggtag 4741 ctgcggttga agcaatgaac tggcttaagt ctgctgctaa gctgctggct gctgaggtca 4801 aagataagaa gactggagag attcttcgca agcgttgcgc tgtgcattgg gtaactcctg 4861 atggtttccc tgtgtggcag gaatacaaga agcctattca gacgcgcttg aacctgatgt 4921 tcctcggtca gttccgctta cagcctacca ttaacaccaa caaagatagc gagattgatg 4981 cacacaaaca ggagtctggt atcgctccta actttgtaca cagccaagac ggtagccacc 5041 ttcgtaagac tgtagtgtgg gcacacgaga agtacggaat cgaatctttt gcactgattc 5101 acgactcctt cggtaccatt ccggctgacg ctgcgaacct gttcaaagca gtgcgcgaaa 5161 ctatggttga cacatatgag tcttgtgatg tactggctga tttctacgac cagttcgctg 5221 accagttgca cgagtctcaa ttggacaaaa tgccagcact tccggctaaa ggtaacttga 5281 acctccgtga catcttagag tcggacttcg cgttcgcata atactagagc caggcatcaa 5341 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg 5401 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata //