LOCUS BBa_K233001 4312 bp DNA linear 14-May-2024 DEFINITION Snowdrift 1 , using TorA. A module to secrete B-galactosidase using the TAT-export Pathway ACCESSION BBa_K233001 VERSION BBa_K233001.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 RBS 209..223 /label=RBS-1\Strong RBS 216..220 /label=RBS misc_feature 209..223 /label=BBa_B0030 RBS 209..223 /label=BBa_B0030 misc_feature 230..368 /label=BBa_K233307 misc_feature 3398..3398 /label=C->T CDS 375..3449 /label=lacZ misc_feature 375..377 /label=START misc_feature 3447..3449 /label=STOP CDS 375..3449 /label=BBa_I732005 CDS 3456..4175 /label=GFP protein CDS 3456..4175 /label=BBa_E0040 stem_loop 4195..4238 /label=stem_loop misc_feature 4184..4263 /label=BBa_B0010 terminator 4184..4263 /label=BBa_B0010 stem_loop 4279..4298 /label=T7 TE misc_feature 4305..4305 /label=stop polyA_site 4299..4312 /label=polya misc_feature 4272..4312 /label=BBa_B0012 terminator 4272..4312 /label=BBa_B0012 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagat taaagaggag aaatactaga tgaacaataa 241 cgacctgttc caggcgtctc gtcgccgttt tctggctcaa ctgggtggcc tgaccgttgc 301 aggtatgctg ggtccgtctc tgctgactcc gcgccgtgct actgccgcgc aagcggctac 361 tgacgctcta ctagatgacc atgattacgg attcactggc cgtcgtttta caacgtcgtg 421 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca 481 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga 541 atggcgaatg gcgctttgcc tggtttccgg caccagaagc ggtgccggaa agctggctgg 601 agtgcgatct tcctgaggcc gatactgtcg tcgtcccctc aaactggcag atgcacggtt 661 acgatgcgcc catctacacc aacgtgacct atcccattac ggtcaatccg ccgtttgttc 721 ccacggagaa tccgacgggt tgttactcgc tcacatttaa tgttgatgaa agctggctac 781 aggaaggcca gacgcgaatt atttttgatg gcgttaactc ggcgtttcat ctgtggtgca 841 acgggcgctg ggtcggttac ggccaggaca gtcgtttgcc gtctgaattt gacctgagcg 901 catttttacg cgccggagaa aaccgcctcg cggtgatggt gctgcgctgg agtgacggca 961 gttatctgga agatcaggat atgtggcgga tgagcggcat tttccgtgac gtctcgttgc 1021 tgcataaacc gactacacaa atcagcgatt tccatgttgc cactcgcttt aatgatgatt 1081 tcagccgcgc tgtactggag gctgaagttc agatgtgcgg cgagttgcgt gactacctac 1141 gggtaacagt ttctttatgg cagggtgaaa cgcaggtcgc cagcggcacc gcgcctttcg 1201 gcggtgaaat tatcgatgag cgtggtggtt atgccgatcg cgtcacacta cgtctgaacg 1261 tcgaaaaccc gaaactgtgg agcgccgaaa tcccgaatct ctatcgtgcg gtggttgaac 1321 tgcacaccgc cgacggcacg ctgattgaag cagaagcctg cgatgtcggt ttccgcgagg 1381 tgcggattga aaatggtctg ctgctgctga acggcaagcc gttgctgatt cgaggcgtta 1441 accgtcacga gcatcatcct ctgcatggtc aggtcatgga tgagcagacg atggtgcagg 1501 atatcctgct gatgaagcag aacaacttta acgccgtgcg ctgttcgcat tatccgaacc 1561 atccgctgtg gtacacgctg tgcgaccgct acggcctgta tgtggtggat gaagccaata 1621 ttgaaaccca cggcatggtg ccaatgaatc gtctgaccga tgatccgcgc tggctaccgg 1681 cgatgagcga acgcgtaacg cgaatggtgc agcgcgatcg taatcacccg agtgtgatca 1741 tctggtcgct ggggaatgaa tcaggccacg gcgctaatca cgacgcgctg tatcgctgga 1801 tcaaatctgt cgatccttcc cgcccggtgc agtatgaagg cggcggagcc gacaccacgg 1861 ccaccgatat tatttgcccg atgtacgcgc gcgtggatga agaccagccc ttcccggctg 1921 tgccgaaatg gtccatcaaa aaatggcttt cgctacctgg agagacgcgc ccgctgatcc 1981 tttgcgaata cgcccacgcg atgggtaaca gtcttggcgg tttcgctaaa tactggcagg 2041 cgtttcgtca gtatccccgt ttacagggcg gcttcgtctg ggactgggtg gatcagtcgc 2101 tgattaaata tgatgaaaac ggcaacccgt ggtcggctta cggcggtgat tttggcgata 2161 cgccgaacga tcgccagttc tgtatgaacg gtctggtctt tgccgaccgc acgccgcatc 2221 cagcgctgac ggaagcaaaa caccagcagc agtttttcca gttccgttta tccgggcaaa 2281 ccatcgaagt gaccagcgaa tacctgttcc gtcatagcga taacgagctc ctgcactgga 2341 tggtggcgct ggatggtaag ccgctggcaa gcggtgaagt gcctctggat gtcgctccac 2401 aaggtaaaca gttgattgaa ctgcctgaac taccgcagcc ggagagcgcc gggcaactct 2461 ggctcacagt acgcgtagtg caaccgaacg cgaccgcatg gtcagaagcc gggcacatca 2521 gcgcctggca gcagtggcgt ctggcggaaa acctcagtgt gacgctcccc gccgcgtccc 2581 acgccatccc gcatctgacc accagcgaaa tggatttttg catcgagctg ggtaataagc 2641 gttggcaatt taaccgccag tcaggctttc tttcacagat gtggattggc gataaaaaac 2701 aactgctgac gccgctgcgc gatcagttca cccgtgcacc gctggataac gacattggcg 2761 taagtgaagc gacccgcatt gaccctaacg cctgggtcga acgctggaag gcggcgggcc 2821 attaccaggc cgaagcagcg ttgttgcagt gcacggcaga tacacttgct gatgcggtgc 2881 tgattacgac cgctcacgcg tggcagcatc aggggaaaac cttatttatc agccggaaaa 2941 cctaccggat tgatggtagt ggtcaaatgg cgattaccgt tgatgttgaa gtggcgagcg 3001 atacaccgca tccggcgcgg attggcctga actgccagct ggcgcaggta gcagagcggg 3061 taaactggct cggattaggg ccgcaagaaa actatcccga ccgccttact gccgcctgtt 3121 ttgaccgctg ggatctgcca ttgtcagaca tgtatacccc gtacgtcttc ccgagcgaaa 3181 acggtctgcg ctgcgggacg cgcgaattga attatggccc acaccagtgg cgcggcgact 3241 tccagttcaa catcagccgc tacagtcaac agcaactgat ggaaaccagc catcgccatc 3301 tgctgcacgc ggaagaaggc acatggctga atatcgacgg tttccatatg gggattggtg 3361 gcgacgactc ctggagcccg tcagtatcgg cggaatttca gctgagcgcc ggtcgctacc 3421 attaccagtt ggtctggtgt caaaaataat actagatgcg taaaggagaa gaacttttca 3481 ctggagttgt cccaattctt gttgaattag atggtgatgt taatgggcac aaattttctg 3541 tcagtggaga gggtgaaggt gatgcaacat acggaaaact tacccttaaa tttatttgca 3601 ctactggaaa actacctgtt ccatggccaa cacttgtcac tactttcggt tatggtgttc 3661 aatgctttgc gagataccca gatcatatga aacagcatga ctttttcaag agtgccatgc 3721 ccgaaggtta tgtacaggaa agaactatat ttttcaaaga tgacgggaac tacaagacac 3781 gtgctgaagt caagtttgaa ggtgataccc ttgttaatag aatcgagtta aaaggtattg 3841 attttaaaga agatggaaac attcttggac acaaattgga atacaactat aactcacaca 3901 atgtatacat catggcagac aaacaaaaga atggaatcaa agttaacttc aaaattagac 3961 acaacattga agatggaagc gttcaactag cagaccatta tcaacaaaat actccaattg 4021 gcgatggccc tgtcctttta ccagacaacc attacctgtc cacacaatct gccctttcga 4081 aagatcccaa cgaaaagaga gaccacatgg tccttcttga gtttgtaaca gctgctggga 4141 ttacacatgg catggatgaa ctatacaaat aataatacta gagccaggca tcaaataaaa 4201 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct 4261 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta //