##gff-version 3
##sequence-region BBa_K233001 1 4312
BBa_K233001	.	engineered_region	230	368	.	+	0	ID=BBa_K233307;Name=BBa_K233307
BBa_K233001	.	CDS	375	3449	.	+	0	ID=BBa_I732005;Name=BBa_I732005
BBa_K233001	.	CDS	375	3449	.	+	0	ID=annotation1936870;Name=lacZ;Parent=BBa_I732005
BBa_K233001	.	point_mutation	3398	3398	.	+	0	ID=annotation1936869;Name=C->T;Parent=BBa_I732005
BBa_K233001	.	start_codon	375	377	.	+	0	ID=annotation1936871;Name=START;Parent=BBa_I732005
BBa_K233001	.	stop_codon	3447	3449	.	+	0	ID=annotation1936872;Name=STOP;Parent=BBa_I732005
BBa_K233001	.	ribosome_entry_site	209	223	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_K233001	.	ribosome_entry_site	216	220	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K233001	.	engineered_region	209	223	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K233001	.	ribosome_entry_site	209	223	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K233001	.	terminator	4184	4263	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K233001	.	engineered_region	4184	4263	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K233001	.	stem_loop	4195	4238	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K233001	.	CDS	3456	4175	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_K233001	.	CDS	3456	4175	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K233001	.	terminator	4272	4312	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K233001	.	engineered_region	4272	4312	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K233001	.	stem_loop	4279	4298	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K233001	.	stop_codon	4305	4305	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K233001	.	polyA_site	4299	4312	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K233001	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_K233001	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K233001	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K233001	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K233001	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K233001	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K233001	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K233001	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
>BBa_K233001
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagattaaagaggagaaatactagatgaacaa
taacgacctgttccaggcgtctcgtcgccgttttctggctcaactgggtggcctgaccgttgcaggtatgctgggtccg
tctctgctgactccgcgccgtgctactgccgcgcaagcggctactgacgctctactagatgaccatgattacggattca
ctggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctt
tcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcg
ctttgcctggtttccggcaccagaagcggtgccggaaagctggctggagtgcgatcttcctgaggccgatactgtcgtc
gtcccctcaaactggcagatgcacggttacgatgcgcccatctacaccaacgtgacctatcccattacggtcaatccgc
cgtttgttcccacggagaatccgacgggttgttactcgctcacatttaatgttgatgaaagctggctacaggaaggcca
gacgcgaattatttttgatggcgttaactcggcgtttcatctgtggtgcaacgggcgctgggtcggttacggccaggac
agtcgtttgccgtctgaatttgacctgagcgcatttttacgcgccggagaaaaccgcctcgcggtgatggtgctgcgct
ggagtgacggcagttatctggaagatcaggatatgtggcggatgagcggcattttccgtgacgtctcgttgctgcataa
accgactacacaaatcagcgatttccatgttgccactcgctttaatgatgatttcagccgcgctgtactggaggctgaa
gttcagatgtgcggcgagttgcgtgactacctacgggtaacagtttctttatggcagggtgaaacgcaggtcgccagcg
gcaccgcgcctttcggcggtgaaattatcgatgagcgtggtggttatgccgatcgcgtcacactacgtctgaacgtcga
aaacccgaaactgtggagcgccgaaatcccgaatctctatcgtgcggtggttgaactgcacaccgccgacggcacgctg
attgaagcagaagcctgcgatgtcggtttccgcgaggtgcggattgaaaatggtctgctgctgctgaacggcaagccgt
tgctgattcgaggcgttaaccgtcacgagcatcatcctctgcatggtcaggtcatggatgagcagacgatggtgcagga
tatcctgctgatgaagcagaacaactttaacgccgtgcgctgttcgcattatccgaaccatccgctgtggtacacgctg
tgcgaccgctacggcctgtatgtggtggatgaagccaatattgaaacccacggcatggtgccaatgaatcgtctgaccg
atgatccgcgctggctaccggcgatgagcgaacgcgtaacgcgaatggtgcagcgcgatcgtaatcacccgagtgtgat
catctggtcgctggggaatgaatcaggccacggcgctaatcacgacgcgctgtatcgctggatcaaatctgtcgatcct
tcccgcccggtgcagtatgaaggcggcggagccgacaccacggccaccgatattatttgcccgatgtacgcgcgcgtgg
atgaagaccagcccttcccggctgtgccgaaatggtccatcaaaaaatggctttcgctacctggagagacgcgcccgct
gatcctttgcgaatacgcccacgcgatgggtaacagtcttggcggtttcgctaaatactggcaggcgtttcgtcagtat
ccccgtttacagggcggcttcgtctgggactgggtggatcagtcgctgattaaatatgatgaaaacggcaacccgtggt
cggcttacggcggtgattttggcgatacgccgaacgatcgccagttctgtatgaacggtctggtctttgccgaccgcac
gccgcatccagcgctgacggaagcaaaacaccagcagcagtttttccagttccgtttatccgggcaaaccatcgaagtg
accagcgaatacctgttccgtcatagcgataacgagctcctgcactggatggtggcgctggatggtaagccgctggcaa
gcggtgaagtgcctctggatgtcgctccacaaggtaaacagttgattgaactgcctgaactaccgcagccggagagcgc
cgggcaactctggctcacagtacgcgtagtgcaaccgaacgcgaccgcatggtcagaagccgggcacatcagcgcctgg
cagcagtggcgtctggcggaaaacctcagtgtgacgctccccgccgcgtcccacgccatcccgcatctgaccaccagcg
aaatggatttttgcatcgagctgggtaataagcgttggcaatttaaccgccagtcaggctttctttcacagatgtggat
tggcgataaaaaacaactgctgacgccgctgcgcgatcagttcacccgtgcaccgctggataacgacattggcgtaagt
gaagcgacccgcattgaccctaacgcctgggtcgaacgctggaaggcggcgggccattaccaggccgaagcagcgttgt
tgcagtgcacggcagatacacttgctgatgcggtgctgattacgaccgctcacgcgtggcagcatcaggggaaaacctt
atttatcagccggaaaacctaccggattgatggtagtggtcaaatggcgattaccgttgatgttgaagtggcgagcgat
acaccgcatccggcgcggattggcctgaactgccagctggcgcaggtagcagagcgggtaaactggctcggattagggc
cgcaagaaaactatcccgaccgccttactgccgcctgttttgaccgctgggatctgccattgtcagacatgtatacccc
gtacgtcttcccgagcgaaaacggtctgcgctgcgggacgcgcgaattgaattatggcccacaccagtggcgcggcgac
ttccagttcaacatcagccgctacagtcaacagcaactgatggaaaccagccatcgccatctgctgcacgcggaagaag
gcacatggctgaatatcgacggtttccatatggggattggtggcgacgactcctggagcccgtcagtatcggcggaatt
tcagctgagcgccggtcgctaccattaccagttggtctggtgtcaaaaataatactagatgcgtaaaggagaagaactt
ttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtg
aaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacact
tgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagt
gccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtca
agtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggaca
caaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttc
aaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggcc
ctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacat
ggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccag
gcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctac
tagagtcacactggctcaccttcgggtgggcctttctgcgtttata