LOCUS BBa_K233002 4131 bp DNA linear 22-Dec-2024 DEFINITION Snowdrift 2 , using YcdB. A module to secrete B-galactosidase using the TAT-export Pathway ACCESSION BBa_K233002 VERSION BBa_K233002.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23118 RBS 44..58 /label=RBS-1\Strong RBS 51..55 /label=RBS misc_feature 44..58 /label=BBa_B0030 RBS 44..58 /label=BBa_B0030 misc_feature 65..187 /label=BBa_K233306 misc_feature 3217..3217 /label=C->T CDS 194..3268 /label=lacZ misc_feature 194..196 /label=START misc_feature 3266..3268 /label=STOP CDS 194..3268 /label=BBa_I732005 CDS 3275..3994 /label=GFP protein CDS 3275..3994 /label=BBa_E0040 stem_loop 4014..4057 /label=stem_loop misc_feature 4003..4082 /label=BBa_B0010 terminator 4003..4082 /label=BBa_B0010 stem_loop 4098..4117 /label=T7 TE misc_feature 4124..4124 /label=stop polyA_site 4118..4131 /label=polya misc_feature 4091..4131 /label=BBa_B0012 terminator 4091..4131 /label=BBa_B0012 ORIGIN 1 ttgacggcta gctcagtcct aggtattgtg ctagctacta gagattaaag aggagaaata 61 ctagatgcag tacaaagacg aaaacggtgt taatgagccg tctcgtcgcc gtctgctgaa 121 ggttatcggc gcgctggctc tggcaggttc ctgcccggtg gcgcatgcgc agaaaactca 181 atctgcctac tagatgacca tgattacgga ttcactggcc gtcgttttac aacgtcgtga 241 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag 301 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa 361 tggcgaatgg cgctttgcct ggtttccggc accagaagcg gtgccggaaa gctggctgga 421 gtgcgatctt cctgaggccg atactgtcgt cgtcccctca aactggcaga tgcacggtta 481 cgatgcgccc atctacacca acgtgaccta tcccattacg gtcaatccgc cgtttgttcc 541 cacggagaat ccgacgggtt gttactcgct cacatttaat gttgatgaaa gctggctaca 601 ggaaggccag acgcgaatta tttttgatgg cgttaactcg gcgtttcatc tgtggtgcaa 661 cgggcgctgg gtcggttacg gccaggacag tcgtttgccg tctgaatttg acctgagcgc 721 atttttacgc gccggagaaa accgcctcgc ggtgatggtg ctgcgctgga gtgacggcag 781 ttatctggaa gatcaggata tgtggcggat gagcggcatt ttccgtgacg tctcgttgct 841 gcataaaccg actacacaaa tcagcgattt ccatgttgcc actcgcttta atgatgattt 901 cagccgcgct gtactggagg ctgaagttca gatgtgcggc gagttgcgtg actacctacg 961 ggtaacagtt tctttatggc agggtgaaac gcaggtcgcc agcggcaccg cgcctttcgg 1021 cggtgaaatt atcgatgagc gtggtggtta tgccgatcgc gtcacactac gtctgaacgt 1081 cgaaaacccg aaactgtgga gcgccgaaat cccgaatctc tatcgtgcgg tggttgaact 1141 gcacaccgcc gacggcacgc tgattgaagc agaagcctgc gatgtcggtt tccgcgaggt 1201 gcggattgaa aatggtctgc tgctgctgaa cggcaagccg ttgctgattc gaggcgttaa 1261 ccgtcacgag catcatcctc tgcatggtca ggtcatggat gagcagacga tggtgcagga 1321 tatcctgctg atgaagcaga acaactttaa cgccgtgcgc tgttcgcatt atccgaacca 1381 tccgctgtgg tacacgctgt gcgaccgcta cggcctgtat gtggtggatg aagccaatat 1441 tgaaacccac ggcatggtgc caatgaatcg tctgaccgat gatccgcgct ggctaccggc 1501 gatgagcgaa cgcgtaacgc gaatggtgca gcgcgatcgt aatcacccga gtgtgatcat 1561 ctggtcgctg gggaatgaat caggccacgg cgctaatcac gacgcgctgt atcgctggat 1621 caaatctgtc gatccttccc gcccggtgca gtatgaaggc ggcggagccg acaccacggc 1681 caccgatatt atttgcccga tgtacgcgcg cgtggatgaa gaccagccct tcccggctgt 1741 gccgaaatgg tccatcaaaa aatggctttc gctacctgga gagacgcgcc cgctgatcct 1801 ttgcgaatac gcccacgcga tgggtaacag tcttggcggt ttcgctaaat actggcaggc 1861 gtttcgtcag tatccccgtt tacagggcgg cttcgtctgg gactgggtgg atcagtcgct 1921 gattaaatat gatgaaaacg gcaacccgtg gtcggcttac ggcggtgatt ttggcgatac 1981 gccgaacgat cgccagttct gtatgaacgg tctggtcttt gccgaccgca cgccgcatcc 2041 agcgctgacg gaagcaaaac accagcagca gtttttccag ttccgtttat ccgggcaaac 2101 catcgaagtg accagcgaat acctgttccg tcatagcgat aacgagctcc tgcactggat 2161 ggtggcgctg gatggtaagc cgctggcaag cggtgaagtg cctctggatg tcgctccaca 2221 aggtaaacag ttgattgaac tgcctgaact accgcagccg gagagcgccg ggcaactctg 2281 gctcacagta cgcgtagtgc aaccgaacgc gaccgcatgg tcagaagccg ggcacatcag 2341 cgcctggcag cagtggcgtc tggcggaaaa cctcagtgtg acgctccccg ccgcgtccca 2401 cgccatcccg catctgacca ccagcgaaat ggatttttgc atcgagctgg gtaataagcg 2461 ttggcaattt aaccgccagt caggctttct ttcacagatg tggattggcg ataaaaaaca 2521 actgctgacg ccgctgcgcg atcagttcac ccgtgcaccg ctggataacg acattggcgt 2581 aagtgaagcg acccgcattg accctaacgc ctgggtcgaa cgctggaagg cggcgggcca 2641 ttaccaggcc gaagcagcgt tgttgcagtg cacggcagat acacttgctg atgcggtgct 2701 gattacgacc gctcacgcgt ggcagcatca ggggaaaacc ttatttatca gccggaaaac 2761 ctaccggatt gatggtagtg gtcaaatggc gattaccgtt gatgttgaag tggcgagcga 2821 tacaccgcat ccggcgcgga ttggcctgaa ctgccagctg gcgcaggtag cagagcgggt 2881 aaactggctc ggattagggc cgcaagaaaa ctatcccgac cgccttactg ccgcctgttt 2941 tgaccgctgg gatctgccat tgtcagacat gtataccccg tacgtcttcc cgagcgaaaa 3001 cggtctgcgc tgcgggacgc gcgaattgaa ttatggccca caccagtggc gcggcgactt 3061 ccagttcaac atcagccgct acagtcaaca gcaactgatg gaaaccagcc atcgccatct 3121 gctgcacgcg gaagaaggca catggctgaa tatcgacggt ttccatatgg ggattggtgg 3181 cgacgactcc tggagcccgt cagtatcggc ggaatttcag ctgagcgccg gtcgctacca 3241 ttaccagttg gtctggtgtc aaaaataata ctagatgcgt aaaggagaag aacttttcac 3301 tggagttgtc ccaattcttg ttgaattaga tggtgatgtt aatgggcaca aattttctgt 3361 cagtggagag ggtgaaggtg atgcaacata cggaaaactt acccttaaat ttatttgcac 3421 tactggaaaa ctacctgttc catggccaac acttgtcact actttcggtt atggtgttca 3481 atgctttgcg agatacccag atcatatgaa acagcatgac tttttcaaga gtgccatgcc 3541 cgaaggttat gtacaggaaa gaactatatt tttcaaagat gacgggaact acaagacacg 3601 tgctgaagtc aagtttgaag gtgataccct tgttaataga atcgagttaa aaggtattga 3661 ttttaaagaa gatggaaaca ttcttggaca caaattggaa tacaactata actcacacaa 3721 tgtatacatc atggcagaca aacaaaagaa tggaatcaaa gttaacttca aaattagaca 3781 caacattgaa gatggaagcg ttcaactagc agaccattat caacaaaata ctccaattgg 3841 cgatggccct gtccttttac cagacaacca ttacctgtcc acacaatctg ccctttcgaa 3901 agatcccaac gaaaagagag accacatggt ccttcttgag tttgtaacag ctgctgggat 3961 tacacatggc atggatgaac tatacaaata ataatactag agccaggcat caaataaaac 4021 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc 4081 tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a //