LOCUS       BBa_K233006             2922 bp    DNA     linear       05-Nov-2024
DEFINITION  AND Gate 3
ACCESSION   BBa_K233006
VERSION     BBa_K233006.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23101
     RBS             44..58
                     /label=RBS-1\Strong
     RBS             51..55
                     /label=RBS
     misc_feature    44..58
                     /label=BBa_B0030
     RBS             44..58
                     /label=BBa_B0030
     misc_feature    65..2716
                     /label=T7rnap ATG Start
     misc_feature    65..2716
                     /label=BBa_I716103
     stem_loop       2736..2779
                     /label=stem_loop
     misc_feature    2725..2804
                     /label=BBa_B0010
     terminator      2725..2804
                     /label=BBa_B0010
     stem_loop       2820..2839
                     /label=T7 TE
     misc_feature    2846..2846
                     /label=stop
     polyA_site      2840..2853
                     /label=polya
     misc_feature    2813..2853
                     /label=BBa_B0012
     terminator      2813..2853
                     /label=BBa_B0012
     promoter        2862..2884
                     /label=BBa_I719005
     promoter        2893..2922
                     /label=BBa_K233004
ORIGIN
        1 tttacagcta gctcagtcct aggtattatg ctagctacta gagattaaag aggagaaata
       61 ctagatgaac acgattaaca tcgctaagaa cgacttctct gacatcgaac tggctgctat
      121 cccgttcaac actctggctg accattacgg tgagcgttta gctcgcgaac agttggccct
      181 tgagcatgag tcttacgaga tgggtgaagc acgcttccgc aagatgtttg agcgtcaact
      241 taaagctggt gaggttgcgg ataacgctgc cgccaagcct ctcatcacta ccctactccc
      301 taagatgatt gcacgcatca acgactggtt tgaggaagtg aaagctaagc gcggcaagcg
      361 cccgacagcc ttccagttcc tgcaagaaat caagccggaa gccgtagcgt acatcaccat
      421 taagaccact ctggcttgcc taaccagtgc tgacaataca accgttcagg ctgtagcaag
      481 cgcaatcggt cgggccattg aggacgaggc tcgcttcggt cgtatccgtg accttgaagc
      541 taagcacttc aagaaaaacg ttgaggaaca actcaacaag cgcgtagggc acgtctacaa
      601 gaaagcattt atgcaagttg tcgaggctga catgctctct aagggtctac tcggtggcga
      661 ggcgtggtct tcgtggcata aggaagactc tattcatgta ggagtacgct gcatcgagat
      721 gctcattgag tcaaccggaa tggttagctt acaccgccaa aatgctggcg tagtaggtca
      781 agactctgag actatcgaac tcgcacctga atacgctgag gctatcgcaa cccgtgcagg
      841 tgcgctggct ggcatctctc cgatgttcca accttgcgta gttcctccta agccgtggac
      901 tggcattact ggtggtggct attgggctaa cggtcgtcgt cctctggcgc tggtgcgtac
      961 tcacagtaag aaagcactga tgcgctacga agacgtttac atgcctgagg tgtacaaagc
     1021 gattaacatt gcgcaaaaca ccgcatggaa aatcaacaag aaagtcctag cggtcgccaa
     1081 cgtaatcacc aagtggaagc attgtccggt cgaggacatc cctgcgattg agcgtgaaga
     1141 actcccgatg aaaccggaag acatcgacat gaatcctgag gctctcaccg cgtggaaacg
     1201 tgctgccgct gctgtgtacc gcaaggacaa ggctcgcaag tctcgccgta tcagccttga
     1261 gttcatgctt gagcaagcca ataagtttgc taaccataag gccatctggt tcccttacaa
     1321 catggactgg cgcggtcgtg tttacgctgt gtcaatgttc aacccgcaag gtaacgatat
     1381 gaccaaagga ctgcttacgc tggcgaaagg taaaccaatc ggtaaggaag gttactactg
     1441 gctgaaaatc cacggtgcaa actgtgcggg tgtcgataag gttccgttcc ctgagcgcat
     1501 caagttcatt gaggaaaacc acgagaacat catggcttgc gctaagtctc cactggagaa
     1561 cacttggtgg gctgagcaag attctccgtt ctgcttcctt gcgttctgct ttgagtacgc
     1621 tggggtacag caccacggcc tgagctataa ctgctccctt ccgctggcgt ttgacgggtc
     1681 ttgctctggc atccagcact tctccgcgat gctccgagat gaggtaggtg gtcgcgcggt
     1741 taacttgctt cctagtgaaa ccgttcagga catctacggg attgttgcta agaaagtcaa
     1801 cgagattcta caagcagacg caatcaatgg gaccgataac gaagtagtta ccgtgaccga
     1861 tgagaacact ggtgaaatct ctgagaaagt caagctgggc actaaggcac tggctggtca
     1921 atggctggct tacggtgtta ctcgcagtgt gactaagcgt tcagtcatga cgctggctta
     1981 cgggtccaaa gagttcggct tccgtcaaca agtgctggaa gataccattc agccagctat
     2041 tgattccggc aagggtctga tgttcactca gccgaatcag gctgctggat acatggctaa
     2101 gctgatttgg gaatctgtga gcgtgacggt ggtagctgcg gttgaagcaa tgaactggct
     2161 taagtctgct gctaagctgc tggctgctga ggtcaaagat aagaagactg gagagattct
     2221 tcgcaagcgt tgcgctgtgc attgggtaac tcctgatggt ttccctgtgt ggcaggaata
     2281 caagaagcct attcagacgc gcttgaacct gatgttcctc ggtcagttcc gcttacagcc
     2341 taccattaac accaacaaag atagcgagat tgatgcacac aaacaggagt ctggtatcgc
     2401 tcctaacttt gtacacagcc aagacggtag ccaccttcgt aagactgtag tgtgggcaca
     2461 cgagaagtac ggaatcgaat cttttgcact gattcacgac tccttcggta ccattccggc
     2521 tgacgctgcg aacctgttca aagcagtgcg cgaaactatg gttgacacat atgagtcttg
     2581 tgatgtactg gctgatttct acgaccagtt cgctgaccag ttgcacgagt ctcaattgga
     2641 caaaatgcca gcacttccgg ctaaaggtaa cttgaacctc cgtgacatct tagagtcgga
     2701 cttcgcgttc gcgtaatact agagccaggc atcaaataaa acgaaaggct cagtcgaaag
     2761 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg
     2821 gctcaccttc gggtgggcct ttctgcgttt atatactaga gtaatacgac tcactatagg
     2881 gagatactag agtgtggaat tgtgagcgct cacaattcca ca
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