##gff-version 3
##sequence-region BBa_K233314 1 881
BBa_K233314	.	engineered_region	83	881	.	+	0	ID=BBa_J37033;Name=BBa_J37033
BBa_K233314	.	CDS	101	856	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_J37033
BBa_K233314	.	engineered_region	101	102	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K233314	.	engineered_tag	857	881	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K233314	.	point_mutation	592	592	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K233314	.	CDS	101	850	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K233314	.	engineered_region	101	856	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K233314	.	point_mutation	274	274	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K233314	.	ribosome_entry_site	83	94	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J37033
BBa_K233314	.	conserved_region	87	90	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K233314	.	promoter	1	74	.	+	0	ID=BBa_J13002;Name=BBa_J13002
BBa_K233314	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J13002
BBa_K233314	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K233314	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_J13002
BBa_K233314	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K233314	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K233314	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K233314	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K233314	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
>BBa_K233314
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgta
gaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcat
ttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgac
gctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaa
acaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccc
tattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttattt
ttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataata
aatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttc
aaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgc
tgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgct
agtgtagatcac