LOCUS       BBa_K233318             1231 bp    DNA     linear       22-Dec-2024
DEFINITION  A tester for Snowdrift 1.
ACCESSION   BBa_K233318
VERSION     BBa_K233318.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     RBS             209..223
                     /label=RBS-1\Strong
     RBS             216..220
                     /label=RBS
     misc_feature    209..223
                     /label=BBa_B0030
     RBS             209..223
                     /label=BBa_B0030
     misc_feature    230..368
                     /label=BBa_K233307
     CDS             375..1094
                     /label=GFP protein
     CDS             375..1094
                     /label=BBa_E0040
     stem_loop       1114..1157
                     /label=stem_loop
     misc_feature    1103..1182
                     /label=BBa_B0010
     terminator      1103..1182
                     /label=BBa_B0010
     stem_loop       1198..1217
                     /label=T7 TE
     misc_feature    1224..1224
                     /label=stop
     polyA_site      1218..1231
                     /label=polya
     misc_feature    1191..1231
                     /label=BBa_B0012
     terminator      1191..1231
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagat taaagaggag aaatactaga tgaacaataa
      241 cgacctgttc caggcgtctc gtcgccgttt tctggctcaa ctgggtggcc tgaccgttgc
      301 aggtatgctg ggtccgtctc tgctgactcc gcgccgtgct actgccgcgc aagcggctac
      361 tgacgctcta ctagatgcgt aaaggagaag aacttttcac tggagttgtc ccaattcttg
      421 ttgaattaga tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg
      481 atgcaacata cggaaaactt acccttaaat ttatttgcac tactggaaaa ctacctgttc
      541 catggccaac acttgtcact actttcggtt atggtgttca atgctttgcg agatacccag
      601 atcatatgaa acagcatgac tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa
      661 gaactatatt tttcaaagat gacgggaact acaagacacg tgctgaagtc aagtttgaag
      721 gtgataccct tgttaataga atcgagttaa aaggtattga ttttaaagaa gatggaaaca
      781 ttcttggaca caaattggaa tacaactata actcacacaa tgtatacatc atggcagaca
      841 aacaaaagaa tggaatcaaa gttaacttca aaattagaca caacattgaa gatggaagcg
      901 ttcaactagc agaccattat caacaaaata ctccaattgg cgatggccct gtccttttac
      961 cagacaacca ttacctgtcc acacaatctg ccctttcgaa agatcccaac gaaaagagag
     1021 accacatggt ccttcttgag tttgtaacag ctgctgggat tacacatggc atggatgaac
     1081 tatacaaata ataatactag agccaggcat caaataaaac gaaaggctca gtcgaaagac
     1141 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc
     1201 tcaccttcgg gtgggccttt ctgcgtttat a
//