LOCUS       BBa_K233319             1215 bp    DNA     linear       22-Dec-2024
DEFINITION  A tester for Snowdrift 2.
ACCESSION   BBa_K233319
VERSION     BBa_K233319.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     RBS             209..223
                     /label=RBS-1\Strong
     RBS             216..220
                     /label=RBS
     misc_feature    209..223
                     /label=BBa_B0030
     RBS             209..223
                     /label=BBa_B0030
     misc_feature    230..352
                     /label=BBa_K233306
     CDS             359..1078
                     /label=GFP protein
     CDS             359..1078
                     /label=BBa_E0040
     stem_loop       1098..1141
                     /label=stem_loop
     misc_feature    1087..1166
                     /label=BBa_B0010
     terminator      1087..1166
                     /label=BBa_B0010
     stem_loop       1182..1201
                     /label=T7 TE
     misc_feature    1208..1208
                     /label=stop
     polyA_site      1202..1215
                     /label=polya
     misc_feature    1175..1215
                     /label=BBa_B0012
     terminator      1175..1215
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagat taaagaggag aaatactaga tgcagtacaa
      241 agacgaaaac ggtgttaatg agccgtctcg tcgccgtctg ctgaaggtta tcggcgcgct
      301 ggctctggca ggttcctgcc cggtggcgca tgcgcagaaa actcaatctg cctactagat
      361 gcgtaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga
      421 tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa
      481 acttaccctt aaatttattt gcactactgg aaaactacct gttccatggc caacacttgt
      541 cactactttc ggttatggtg ttcaatgctt tgcgagatac ccagatcata tgaaacagca
      601 tgactttttc aagagtgcca tgcccgaagg ttatgtacag gaaagaacta tatttttcaa
      661 agatgacggg aactacaaga cacgtgctga agtcaagttt gaaggtgata cccttgttaa
      721 tagaatcgag ttaaaaggta ttgattttaa agaagatgga aacattcttg gacacaaatt
      781 ggaatacaac tataactcac acaatgtata catcatggca gacaaacaaa agaatggaat
      841 caaagttaac ttcaaaatta gacacaacat tgaagatgga agcgttcaac tagcagacca
      901 ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt ttaccagaca accattacct
      961 gtccacacaa tctgcccttt cgaaagatcc caacgaaaag agagaccaca tggtccttct
     1021 tgagtttgta acagctgctg ggattacaca tggcatggat gaactataca aataataata
     1081 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
     1141 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc
     1201 ctttctgcgt ttata
//