##gff-version 3
##sequence-region BBa_K233320 1 2837
BBa_K233320	.	promoter	1	56	.	+	0	ID=BBa_R1062;Name=BBa_R1062
BBa_K233320	.	promoter	42	48	.	+	0	ID=annotation2099;Name=-10;Parent=BBa_R1062
BBa_K233320	.	promoter	20	25	.	+	0	ID=annotation2101;Name=-35;Parent=BBa_R1062
BBa_K233320	.	non_covalent_binding_site	1	20	.	+	0	ID=annotation2098;Name=LuxR/HSL;Parent=BBa_R1062
BBa_K233320	.	start_codon	54	54	.	+	0	ID=annotation2100;Name=start;Parent=BBa_R1062
BBa_K233320	.	engineered_region	1	56	.	+	0	ID=annotation7077;Name=BBa_R1062;Parent=BBa_R1062
BBa_K233320	.	terminator	2709	2837	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K233320	.	terminator	2709	2788	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K233320	.	engineered_region	2709	2788	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K233320	.	stem_loop	2720	2763	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K233320	.	terminator	2797	2837	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K233320	.	engineered_region	2797	2837	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K233320	.	stop_codon	2830	2830	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K233320	.	stem_loop	2804	2823	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K233320	.	polyA_site	2824	2837	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K233320	.	promoter	1858	1913	.	+	0	ID=BBa_R1062;Name=BBa_R1062
BBa_K233320	.	promoter	1899	1905	.	+	0	ID=annotation2099;Name=-10;Parent=BBa_R1062
BBa_K233320	.	promoter	1877	1882	.	+	0	ID=annotation2101;Name=-35;Parent=BBa_R1062
BBa_K233320	.	non_covalent_binding_site	1858	1877	.	+	0	ID=annotation2098;Name=LuxR/HSL;Parent=BBa_R1062
BBa_K233320	.	start_codon	1911	1911	.	+	0	ID=annotation2100;Name=start;Parent=BBa_R1062
BBa_K233320	.	engineered_region	1858	1913	.	+	0	ID=annotation7077;Name=BBa_R1062;Parent=BBa_R1062
BBa_K233320	.	terminator	734	862	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K233320	.	terminator	734	813	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K233320	.	engineered_region	734	813	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K233320	.	stem_loop	745	788	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K233320	.	terminator	822	862	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K233320	.	engineered_region	822	862	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K233320	.	stop_codon	855	855	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K233320	.	stem_loop	829	848	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K233320	.	polyA_site	849	862	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K233320	.	terminator	1721	1849	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K233320	.	terminator	1721	1800	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K233320	.	engineered_region	1721	1800	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K233320	.	stem_loop	1732	1775	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K233320	.	terminator	1809	1849	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K233320	.	engineered_region	1809	1849	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K233320	.	stop_codon	1842	1842	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K233320	.	stem_loop	1816	1835	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K233320	.	polyA_site	1836	1849	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K233320	.	engineered_region	914	1712	.	+	0	ID=BBa_J37033;Name=BBa_J37033
BBa_K233320	.	CDS	932	1687	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_J37033
BBa_K233320	.	point_mutation	1423	1423	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K233320	.	CDS	932	1681	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K233320	.	engineered_region	932	1687	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K233320	.	engineered_tag	1688	1712	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K233320	.	point_mutation	1105	1105	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K233320	.	engineered_region	932	933	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K233320	.	ribosome_entry_site	914	925	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J37033
BBa_K233320	.	conserved_region	918	921	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K233320	.	promoter	1922	1951	.	+	0	ID=BBa_K233004;Name=BBa_K233004
BBa_K233320	.	engineered_region	65	725	.	+	0	ID=BBa_C0261;Name=BBa_C0261
BBa_K233320	.	CDS	83	700	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_C0261
BBa_K233320	.	CDS	83	661	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K233320	.	engineered_tag	701	725	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K233320	.	engineered_region	83	700	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K233320	.	tag	662	693	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K233320	.	ribosome_entry_site	65	76	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_C0261
BBa_K233320	.	conserved_region	69	72	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K233320	.	ribosome_entry_site	1960	1974	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_K233320	.	engineered_region	1960	1974	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K233320	.	ribosome_entry_site	1967	1971	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K233320	.	ribosome_entry_site	1960	1974	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K233320	.	promoter	871	905	.	+	0	ID=BBa_J23102;Name=BBa_J23102
BBa_K233320	.	CDS	1981	2700	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_K233320	.	CDS	1981	2700	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
>BBa_K233320
acctgtaggatcgtacaggttgacacaagaaaatggtttgttgatactcgaataaatactagagaaagaggagaaatac
tagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgtt
atcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaa
tgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattat
atgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgtt
ttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataa
acacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaa
gttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctatta
atgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtg
ctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatct
gttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactaga
gttgacagctagctcagtcctaggtactgtgctagctactagagaaagaggagaaatactagatgaaaaacataaatgc
cgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatg
actaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcc
tagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattc
taactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaa
gaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagtt
ttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttc
tctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgt
ttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttcc
atttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaat
tgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaac
gaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactgg
ctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggttgacacaagaaaatggttt
gttgatactcgaataaatactagagtgtggaattgtgagcgctcacaattccacatactagagattaaagaggagaaat
actagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggca
caaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactgga
aaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcata
tgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgg
gaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgatttt
aaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaac
aaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatca
acaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaa
gatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaac
tatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatct
gttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata