LOCUS BBa_K233321 3392 bp DNA linear 15-Nov-2024 DEFINITION Turing Machine - Unary Adder 2 ACCESSION BBa_K233321 VERSION BBa_K233321.1 FEATURES Location/Qualifiers misc_feature 1..20 /label=LuxR/HSL promoter 42..48 /label=-10 misc_feature 54..54 /label=start promoter 20..25 /label=-35 misc_feature 1..56 /label=BBa_R1062 promoter 1..56 /label=BBa_R1062 misc_feature 69..72 /label=conserved RBS 65..76 /label=BBa_B0034 misc_feature 662..693 /label=LVA CDS 83..661 /label=luxI misc_feature 83..700 /label=BBa_C0061 misc_feature 701..725 /label=Help:Barcodes CDS 83..700 /label=BBa_C0061 misc_feature 65..725 /label=BBa_C0261 stem_loop 745..788 /label=stem_loop misc_feature 734..813 /label=BBa_B0010 terminator 734..813 /label=BBa_B0010 stem_loop 829..848 /label=T7 TE misc_feature 855..855 /label=stop polyA_site 849..862 /label=polya misc_feature 822..862 /label=BBa_B0012 terminator 822..862 /label=BBa_B0012 terminator 734..862 /label=BBa_B0015 promoter 871..905 /label=BBa_J23102 misc_feature 918..921 /label=conserved RBS 914..925 /label=BBa_B0034 misc_feature 932..933 /label=prefix misc_feature 1105..1105 /label=T misc_feature 1423..1423 /label=A CDS 932..1681 /label=luxR misc_feature 932..1687 /label=BBa_C0062 misc_feature 1688..1712 /label=Help:Barcodes CDS 932..1687 /label=BBa_C0062 misc_feature 914..1712 /label=BBa_J37033 stem_loop 1732..1775 /label=stem_loop misc_feature 1721..1800 /label=BBa_B0010 terminator 1721..1800 /label=BBa_B0010 stem_loop 1816..1835 /label=T7 TE misc_feature 1842..1842 /label=stop polyA_site 1836..1849 /label=polya misc_feature 1809..1849 /label=BBa_B0012 terminator 1809..1849 /label=BBa_B0012 terminator 1721..1849 /label=BBa_B0015 misc_feature 1858..1877 /label=LuxR/HSL promoter 1899..1905 /label=-10 misc_feature 1911..1911 /label=start promoter 1877..1882 /label=-35 misc_feature 1858..1913 /label=BBa_R1062 promoter 1858..1913 /label=BBa_R1062 promoter 1922..1951 /label=BBa_K233004 RBS 1960..1974 /label=RBS-1\Strong RBS 1967..1971 /label=RBS misc_feature 1960..1974 /label=BBa_B0030 RBS 1960..1974 /label=BBa_B0030 misc_feature 1983..3255 /label=BBa_K124003 stem_loop 3275..3318 /label=stem_loop misc_feature 3264..3343 /label=BBa_B0010 terminator 3264..3343 /label=BBa_B0010 stem_loop 3359..3378 /label=T7 TE misc_feature 3385..3385 /label=stop polyA_site 3379..3392 /label=polya misc_feature 3352..3392 /label=BBa_B0012 terminator 3352..3392 /label=BBa_B0012 terminator 3264..3392 /label=BBa_B0015 ORIGIN 1 acctgtagga tcgtacaggt tgacacaaga aaatggtttg ttgatactcg aataaatact 61 agagaaagag gagaaatact agatgactat aatgataaaa aaatcggatt ttttggcaat 121 tccatcggag gagtataaag gtattctaag tcttcgttat caagtgttta agcaaagact 181 tgagtgggac ttagttgtag aaaataacct tgaatcagat gagtatgata actcaaatgc 241 agaatatatt tatgcttgtg atgatactga aaatgtaagt ggatgctggc gtttattacc 301 tacaacaggt gattatatgc tgaaaagtgt ttttcctgaa ttgcttggtc aacagagtgc 361 tcccaaagat cctaatatag tcgaattaag tcgttttgct gtaggtaaaa atagctcaaa 421 gataaataac tctgctagtg aaattacaat gaaactattt gaagctatat ataaacacgc 481 tgttagtcaa ggtattacag aatatgtaac agtaacatca acagcaatag agcgattttt 541 aaagcgtatt aaagttcctt gtcatcgtat tggagacaaa gaaattcatg tattaggtga 601 tactaaatcg gttgtattgt ctatgcctat taatgaacag tttaaaaaag cagtcttaaa 661 tgctgcaaac gacgaaaact acgctttagt agcttaataa ctctgatagt gctagtgtag 721 atctctacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt 781 cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg 841 ggtgggcctt tctgcgttta tatactagag ttgacagcta gctcagtcct aggtactgtg 901 ctagctacta gagaaagagg agaaatacta gatgaaaaac ataaatgccg acgacacata 961 cagaataatt aataaaatta aagcttgtag aagcaataat gatattaatc aatgcttatc 1021 tgatatgact aaaatggtac attgtgaata ttatttactc gcgatcattt atcctcattc 1081 tatggttaaa tctgatattt caatcctaga taattaccct aaaaaatgga ggcaatatta 1141 tgatgacgct aatttaataa aatatgatcc tatagtagat tattctaact ccaatcattc 1201 accaattaat tggaatatat ttgaaaacaa tgctgtaaat aaaaaatctc caaatgtaat 1261 taaagaagcg aaaacatcag gtcttatcac tgggtttagt ttccctattc atacggctaa 1321 caatggcttc ggaatgctta gttttgcaca ttcagaaaaa gacaactata tagatagttt 1381 atttttacat gcgtgtatga acataccatt aattgttcct tctctagttg ataattatcg 1441 aaaaataaat atagcaaata ataaatcaaa caacgattta accaaaagag aaaaagaatg 1501 tttagcgtgg gcatgcgaag gaaaaagctc ttgggatatt tcaaaaatat taggttgcag 1561 tgagcgtact gtcactttcc atttaaccaa tgcgcaaatg aaactcaata caacaaaccg 1621 ctgccaaagt atttctaaag caattttaac aggagcaatt gattgcccat actttaaaaa 1681 ttaataacac tgatagtgct agtgtagatc actactagag ccaggcatca aataaaacga 1741 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc 1801 tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat actagagacc 1861 tgtaggatcg tacaggttga cacaagaaaa tggtttgttg atactcgaat aaatactaga 1921 gtgtggaatt gtgagcgctc acaattccac atactagaga ttaaagagga gaaatactag 1981 agaattcatc agtgatagtt acgttacgct gcgccttctt tttttccctt ccaatataag 2041 aactacgcaa tccgttactg gcggaggcgt tgctatgaaa tcaatggaca aaatctcaac 2101 tggcattgcc tacggaacat ctgctggtag tgcgggatac tggtttttgc agtggttgga 2161 tcaggtcagt ccgtcacagt gggctgcgat tggagtgctt ggaagccttg tgttgggttt 2221 tctcacttat ctgacaaatc tgtatttcaa aatcagagaa gacagacgaa aggctgcgag 2281 aggtgaataa tgcctccatc attacgaaaa gccgttgctg ctgctattgg tggcggagca 2341 attgctatag catcagtgtt aatcactggc ccaagtggta acgatggtct ggaaggtgtc 2401 agctacatac catacaaaga tattgttggt gtatggactg tatgtcacgg gcatacagga 2461 aaagacatca tgctcggtaa aacgtatacc aaagcagaat gcaaagccct cctgaataaa 2521 gaccttgcca cggtcgccag acaaattaac ccgtacatca aagtcgatat accggaaaca 2581 atgcgcggcg ctctttactc attgctttac aacgtgggtg ctggcaattt cagaacatcg 2641 acgcttcttc gtaaaataaa ccagggcgat atcaaaggcg catgtgatca gctacgtcgc 2701 tggacatatg ctggcggtaa gcaatggaaa ggtctcatga ctcgtcgtga gattgagcgt 2761 gaaatctgtt tgtggggtca gcaatgaaca gagtaaccgc gattatctcc gctctggtta 2821 tctgcatcat cgtctgcctg tcatgggctg ttaatcatta ccgtgataac gccattacct 2881 acaaagccca gcgcgacaaa aatgccagag aactgacgct ggcgaaccgg gtaattactg 2941 acatacagat gcgtcagcgt gatgttgctg cgctcgatgc aaaatacacg aaggagttag 3001 ctgatgcgaa agctgaaaat gatgctctgc gtgatgatgt tgccgctggt cgtcgtcggt 3061 tgcacatcaa agcagtctgt cagtcagtgc gtgaagccac gactgcctcc ggcgtggata 3121 atgcagcctc cccccgactg gcagacaccg ctgaacggga ttatttcacc ctcagagaga 3181 ggctgatcac tatgcaaaaa caactggaag gaacccagaa gtatattaat gagcagtgca 3241 gatagagctg cccattacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga 3301 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg 3361 ctcaccttcg ggtgggcctt tctgcgttta ta //