BBa_K233321
type: ComponentDefinition
persistent identity: BBa K233321
display id: BBa_K233321
version: 1
was generated by: iGEM to SBOL conversion
description: Turing Machine - Unary Adder 2
created: 2009-10-17T11:00:00Z
modified: 2015-05-08T01:11:35Z
mutable provenance: q
owned by: james
owned by: myers
mutable description: a
discontinued: false
dominant: false
experience: experience/None
group u list: _329_
m user id: 0
owner id: 4259
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: e
star: false
creator: swetha srinivasan, chinar patil, mandar phatak, samit watve
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_B0030
component: BBa_K233004
component: BBa_B0015
component: BBa_K124003
component: BBa_R1062
component: BBa_C0261
component: BBa_J37033
component: BBa_J23102
sequence annotation: BBa_R1062
sequence annotation: BBa_C0261
sequence annotation: BBa_B0015
sequence annotation: BBa_J23102
sequence annotation: BBa_J37033
sequence annotation: BBa_K233004
sequence annotation: BBa_B0030
sequence annotation: BBa_K124003
sequence: https://synbiohub.org/public/igem/BBa_K233321_sequence/1

BBa_B0030
type: Component
persistent identity: component2313762
display id: component2313762
version: 1
top level: BBa_K233321
definition: BBa_B0030
access: public

BBa_K233004
type: Component
persistent identity: component2313760
display id: component2313760
version: 1
top level: BBa_K233321
definition: BBa_K233004
access: public

BBa_B0015
type: Component
persistent identity: component2313771
display id: component2313771
version: 1
top level: BBa_K233321
definition: BBa_B0015
access: public

BBa_K124003
type: Component
persistent identity: component2313764
display id: component2313764
version: 1
top level: BBa_K233321
definition: BBa_K124003
access: public

BBa_R1062
type: Component
persistent identity: component2313720
display id: component2313720
version: 1
top level: BBa_K233321
definition: lux pR
access: public

BBa_B0015
type: Component
persistent identity: component2313737
display id: component2313737
version: 1
top level: BBa_K233321
definition: BBa_B0015
access: public

BBa_C0261
type: Component
persistent identity: component2313730
display id: component2313730
version: 1
top level: BBa_K233321
definition: BBa_C0261
access: public

BBa_J37033
type: Component
persistent identity: component2313747
display id: component2313747
version: 1
top level: BBa_K233321
definition: BBa_J37033
access: public

BBa_J23102
type: Component
persistent identity: component2313738
display id: component2313738
version: 1
top level: BBa_K233321
definition: BBa_J23102
access: public

BBa_R1062
type: Component
persistent identity: component2313756
display id: component2313756
version: 1
top level: BBa_K233321
definition: lux pR
access: public

BBa_B0015
type: Component
persistent identity: component2313754
display id: component2313754
version: 1
top level: BBa_K233321
definition: BBa_B0015
access: public

BBa_R1062
type: SequenceAnnotation
persistent identity: annotation2313720
display id: annotation2313720
version: 1
top level: BBa_K233321
location: https://synbiohub.org/public/igem/BBa_K233321/annotation2313720/range2313720/1
component: BBa_R1062

https://synbiohub.org/public/igem/BBa_K233321/annotation2313720/range2313720/1
type: Range
persistent identity: range2313720
display id: range2313720
version: 1
top level: BBa_K233321
direction: direction/forward
start: 1
end: 56
orientation: inline

BBa_C0261
type: SequenceAnnotation
persistent identity: annotation2313730
display id: annotation2313730
version: 1
top level: BBa_K233321
location: https://synbiohub.org/public/igem/BBa_K233321/annotation2313730/range2313730/1
component: BBa_C0261

https://synbiohub.org/public/igem/BBa_K233321/annotation2313730/range2313730/1
type: Range
persistent identity: range2313730
display id: range2313730
version: 1
top level: BBa_K233321
direction: direction/reverse
start: 65
end: 725
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2313737
display id: annotation2313737
version: 1
top level: BBa_K233321
location: https://synbiohub.org/public/igem/BBa_K233321/annotation2313737/range2313737/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K233321/annotation2313737/range2313737/1
type: Range
persistent identity: range2313737
display id: range2313737
version: 1
top level: BBa_K233321
direction: direction/reverse
start: 734
end: 862
orientation: inline

BBa_J23102
type: SequenceAnnotation
persistent identity: annotation2313738
display id: annotation2313738
version: 1
top level: BBa_K233321
location: https://synbiohub.org/public/igem/BBa_K233321/annotation2313738/range2313738/1
component: BBa_J23102

https://synbiohub.org/public/igem/BBa_K233321/annotation2313738/range2313738/1
type: Range
persistent identity: range2313738
display id: range2313738
version: 1
top level: BBa_K233321
direction: direction/reverse
start: 871
end: 905
orientation: inline

BBa_J37033
type: SequenceAnnotation
persistent identity: annotation2313747
display id: annotation2313747
version: 1
top level: BBa_K233321
location: https://synbiohub.org/public/igem/BBa_K233321/annotation2313747/range2313747/1
component: BBa_J37033

https://synbiohub.org/public/igem/BBa_K233321/annotation2313747/range2313747/1
type: Range
persistent identity: range2313747
display id: range2313747
version: 1
top level: BBa_K233321
direction: direction/reverse
start: 914
end: 1712
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2313754
display id: annotation2313754
version: 1
top level: BBa_K233321
location: https://synbiohub.org/public/igem/BBa_K233321/annotation2313754/range2313754/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K233321/annotation2313754/range2313754/1
type: Range
persistent identity: range2313754
display id: range2313754
version: 1
top level: BBa_K233321
direction: direction/reverse
start: 1721
end: 1849
orientation: inline

BBa_R1062
type: SequenceAnnotation
persistent identity: annotation2313756
display id: annotation2313756
version: 1
top level: BBa_K233321
location: https://synbiohub.org/public/igem/BBa_K233321/annotation2313756/range2313756/1
component: BBa_R1062

https://synbiohub.org/public/igem/BBa_K233321/annotation2313756/range2313756/1
type: Range
persistent identity: range2313756
display id: range2313756
version: 1
top level: BBa_K233321
direction: direction/forward
start: 1858
end: 1913
orientation: inline

BBa_K233004
type: SequenceAnnotation
persistent identity: annotation2313760
display id: annotation2313760
version: 1
top level: BBa_K233321
location: https://synbiohub.org/public/igem/BBa_K233321/annotation2313760/range2313760/1
component: BBa_K233004

https://synbiohub.org/public/igem/BBa_K233321/annotation2313760/range2313760/1
type: Range
persistent identity: range2313760
display id: range2313760
version: 1
top level: BBa_K233321
direction: direction/reverse
start: 1922
end: 1951
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2313762
display id: annotation2313762
version: 1
top level: BBa_K233321
location: https://synbiohub.org/public/igem/BBa_K233321/annotation2313762/range2313762/1
component: BBa_B0030

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type: Range
persistent identity: range2313762
display id: range2313762
version: 1
top level: BBa_K233321
direction: direction/forward
start: 1960
end: 1974
orientation: inline

BBa_K124003
type: SequenceAnnotation
persistent identity: annotation2313764
display id: annotation2313764
version: 1
top level: BBa_K233321
location: https://synbiohub.org/public/igem/BBa_K233321/annotation2313764/range2313764/1
component: BBa_K124003

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type: Range
persistent identity: range2313764
display id: range2313764
version: 1
top level: BBa_K233321
direction: direction/reverse
start: 1983
end: 3255
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2313771
display id: annotation2313771
version: 1
top level: BBa_K233321
location: https://synbiohub.org/public/igem/BBa_K233321/annotation2313771/range2313771/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K233321/annotation2313771/range2313771/1
type: Range
persistent identity: range2313771
display id: range2313771
version: 1
top level: BBa_K233321
direction: direction/reverse
start: 3264
end: 3392
orientation: inline

BBa_C0261
type: ComponentDefinition
persistent identity: BBa C0261
display id: BBa_C0261
version: 1
was generated by: iGEM to SBOL conversion
description: AHL-making Enzyme, luxI (+RBS)
created: 2004-08-22T11:00:00Z
modified: 2015-08-31T04:07:24Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: B0034.C0061
discontinued: false
dominant: false
experience: experience/None
group u list: _6_
m user id: 0
owner id: 159
owning group id: 7
sample status: In stock
status: status/Available
bookmark: true
star: true
creator: robertb
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_C0061
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_C0061
sequence: https://synbiohub.org/public/igem/BBa_C0261_sequence/1

BBa_C0061
type: Component
persistent identity: component1060413
display id: component1060413
version: 1
top level: BBa_C0261
definition: luxI
access: public

BBa_B0034
type: Component
persistent identity: component1060403
display id: component1060403
version: 1
top level: BBa_C0261
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1060403
display id: annotation1060403
version: 1
top level: BBa_C0261
location: https://synbiohub.org/public/igem/BBa_C0261/annotation1060403/range1060403/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_C0261/annotation1060403/range1060403/1
type: Range
persistent identity: range1060403
display id: range1060403
version: 1
top level: BBa_C0261
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation1060413
display id: annotation1060413
version: 1
top level: BBa_C0261
location: https://synbiohub.org/public/igem/BBa_C0261/annotation1060413/range1060413/1
component: BBa_C0061

https://synbiohub.org/public/igem/BBa_C0261/annotation1060413/range1060413/1
type: Range
persistent identity: range1060413
display id: range1060413
version: 1
top level: BBa_C0261
direction: direction/forward
start: 19
end: 636
orientation: inline

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.1 (strong) -- modified from R. Weiss
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-1\Strong
sequence annotation: BBa_B0030
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
top level: BBa_B0030
direction: direction/forward
start: 8
end: 12
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_J23102
type: ComponentDefinition
persistent identity: BBa J23102
display id: BBa_J23102
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: isolated from library of promoters
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: replace later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_J23102_sequence/1

BBa_K124003
type: ComponentDefinition
persistent identity: BBa K124003
display id: BBa_K124003
version: 1
was generated by: iGEM to SBOL conversion
description: Bacteriophage 21 Lysis Cassette S, R, and Rz (PVJ4)
created: 2008-07-08T11:00:00Z
modified: 2015-05-08T01:09:44Z
mutable provenance: This part comes from a Lamda Phage provided to us by Harvard Medical School with the help of Ry Young (Texas A&M).
owned by: james
owned by: myers
mutable description: Induces lysis in E. Coli bacteria.
discontinued: false
dominant: false
experience: experience/None
group u list: _221_
m user id: 0
owner id: 3365
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: Not many.
star: true
creator: Neil J. Parikh
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K124003_sequence/1

BBa_J37033
type: ComponentDefinition
persistent identity: BBa J37033
display id: BBa_J37033
version: 1
was generated by: iGEM to SBOL conversion
description: RBS + LuxR
created: 2006-08-22T11:00:00Z
modified: 2015-08-31T04:08:48Z
mutable provenance: Ligation of B0034 + C0062
owned by: james
owned by: myers
mutable description: LuxR coding region with RBS
discontinued: false
dominant: false
experience: experience/None
group u list: _66_
m user id: 0
owner id: 1052
owning group id: 66
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: Designed for Imperial College iGEM 2006
star: true
creator: John Sy
type: DnaRegion
role: SO:0000804
role: partType/Translational Unit
component: BBa_B0034
component: BBa_C0062
sequence annotation: BBa_B0034
sequence annotation: BBa_C0062
sequence: https://synbiohub.org/public/igem/BBa_J37033_sequence/1

BBa_B0034
type: Component
persistent identity: component2243716
display id: component2243716
version: 1
top level: BBa_J37033
definition: BBa_B0034
access: public

BBa_C0062
type: Component
persistent identity: component2243719
display id: component2243719
version: 1
top level: BBa_J37033
definition: luxr
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2243716
display id: annotation2243716
version: 1
top level: BBa_J37033
location: https://synbiohub.org/public/igem/BBa_J37033/annotation2243716/range2243716/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J37033/annotation2243716/range2243716/1
type: Range
persistent identity: range2243716
display id: range2243716
version: 1
top level: BBa_J37033
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation2243719
display id: annotation2243719
version: 1
top level: BBa_J37033
location: https://synbiohub.org/public/igem/BBa_J37033/annotation2243719/range2243719/1
component: BBa_C0062

https://synbiohub.org/public/igem/BBa_J37033/annotation2243719/range2243719/1
type: Range
persistent identity: range2243719
display id: range2243719
version: 1
top level: BBa_J37033
direction: direction/forward
start: 19
end: 774
orientation: inline

BBa_K233004
type: ComponentDefinition
persistent identity: BBa K233004
display id: BBa_K233004
version: 1
was generated by: iGEM to SBOL conversion
description: lac operator
created: 2009-07-09T11:00:00Z
modified: 2015-05-08T01:11:35Z
mutable provenance: E.coli
owned by: james
owned by: myers
mutable description: This contains the lacI binding sites without any of the -10 and -35 regions of the promoter.therefore it is only repressed by the binding of LacI
discontinued: false
dominant: false
experience: experience/None
group u list: _329_
m user id: 0
owner id: 4258
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: had it synthesized
star: false
creator: Swetha Srinivasan, Samit Watve, Mandar Phatak, Chinar Patil
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K233004_sequence/1

lux pR
type: ComponentDefinition
persistent identity: BBa R1062
display id: BBa_R1062
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter, Standard (luxR and HSL regulated -- lux pR)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: V. fischeri
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: [Note: This is the same part as R0062 except that the -10 and -35 sites and spacing have been changed to comply with BBa_S0001]. Promoter activated by LuxR in concert with HSL The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the LuxR activator protein complexed with the autoinducer, 3-oxo-hexanoyl-HSL. Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: This promoter is based on the Vibrio fischeri quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below. <img src="<bb_file part=BBa_R0062>Image1.gif</bb_file>" width="614" height="362"> Modified to comply with BBa_S0001: TTGACA-17N-GATACT
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr Drew Endy
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: LuxR/HSL
sequence annotation: BBa_R1062
sequence annotation: -35
sequence annotation: -10
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R1062_sequence/1

LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2098
display id: annotation2098
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R1062/annotation2098/range2098/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R1062/annotation2098/range2098/1
type: Range
persistent identity: range2098
display id: range2098
version: 1
top level: lux pR
direction: direction/forward
start: 1
end: 20
orientation: inline

BBa_R1062
type: SequenceAnnotation
persistent identity: annotation7077
display id: annotation7077
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R1062/annotation7077/range7077/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_R1062/annotation7077/range7077/1
type: Range
persistent identity: range7077
display id: range7077
version: 1
top level: lux pR
direction: direction/forward
start: 1
end: 56
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2101
display id: annotation2101
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R1062/annotation2101/range2101/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R1062/annotation2101/range2101/1
type: Range
persistent identity: range2101
display id: range2101
version: 1
top level: lux pR
direction: direction/forward
start: 20
end: 25
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2099
display id: annotation2099
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R1062/annotation2099/range2099/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R1062/annotation2099/range2099/1
type: Range
persistent identity: range2099
display id: range2099
version: 1
top level: lux pR
direction: direction/forward
start: 42
end: 48
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2100
display id: annotation2100
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R1062/annotation2100/range2100/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R1062/annotation2100/range2100/1
type: Range
persistent identity: range2100
display id: range2100
version: 1
top level: lux pR
direction: direction/forward
start: 54
end: 54
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

luxI
type: ComponentDefinition
persistent identity: BBa C0061
display id: BBa_C0061
version: 1
was generated by: iGEM to SBOL conversion
description: autoinducer synthetase for AHL
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: V. fischeri <genbank>AF170104</genbank>
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Synthesizes 3OC6HSL, which binds to LuxR. The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux repressor, LuxR. Two molecules of LuxR protein form a complex with two molecules the signalling compound HSL. This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: An LVA tail (sequence: AANDENYALVA) was added to increase protein degradation. .
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: BBa_C0061
sequence annotation: luxI
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0061_sequence/1

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation7038
display id: annotation7038
version: 1
top level: luxI
location: https://synbiohub.org/public/igem/BBa_C0061/annotation7038/range7038/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_C0061/annotation7038/range7038/1
type: Range
persistent identity: range7038
display id: range7038
version: 1
top level: luxI
direction: direction/forward
start: 1
end: 618
orientation: inline

luxI
type: SequenceAnnotation
persistent identity: annotation1761
display id: annotation1761
version: 1
top level: luxI
location: https://synbiohub.org/public/igem/BBa_C0061/annotation1761/range1761/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0061/annotation1761/range1761/1
type: Range
persistent identity: range1761
display id: range1761
version: 1
top level: luxI
direction: direction/forward
start: 1
end: 579
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1760
display id: annotation1760
version: 1
top level: luxI
location: https://synbiohub.org/public/igem/BBa_C0061/annotation1760/range1760/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_C0061/annotation1760/range1760/1
type: Range
persistent identity: range1760
display id: range1760
version: 1
top level: luxI
direction: direction/forward
start: 580
end: 611
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213985
display id: annotation2213985
version: 1
top level: luxI
location: https://synbiohub.org/public/igem/BBa_C0061/annotation2213985/range2213985/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0061/annotation2213985/range2213985/1
type: Range
persistent identity: range2213985
display id: range2213985
version: 1
top level: luxI
direction: direction/forward
start: 619
end: 643
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

luxr
type: ComponentDefinition
persistent identity: BBa C0062
display id: BBa_C0062
version: 1
was generated by: iGEM to SBOL conversion
description: luxR repressor/activator, (no LVA?)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: V. fischeri <genbank>AF170104</genbank>
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: In complex with HSL, LuxR binds to the Lux promoter, activating transcription from Pr <bb_part>BBa_R0062</bb_part>, and repressing transcription from Pl <bb_part>BBa_R0063</bb_part>. The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux activator, LuxR complexed to HSL. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: 2 silent point mutants were introduced in the coding sequence to remove internal XbaI and PstI sites. Mutation sites were chosen to replace codons commonly used in E. coli with codons used at a similar frequency.
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: luxR
sequence annotation: prefix
sequence annotation: BBa_C0062
sequence annotation: T
sequence annotation: A
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0062_sequence/1

luxR
type: SequenceAnnotation
persistent identity: annotation1766
display id: annotation1766
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1766/range1766/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0062/annotation1766/range1766/1
type: Range
persistent identity: range1766
display id: range1766
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 750
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation1762
display id: annotation1762
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1762/range1762/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_C0062/annotation1762/range1762/1
type: Range
persistent identity: range1762
display id: range1762
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 2
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation7039
display id: annotation7039
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation7039/range7039/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_C0062/annotation7039/range7039/1
type: Range
persistent identity: range7039
display id: range7039
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 756
orientation: inline

T
type: SequenceAnnotation
persistent identity: annotation1764
display id: annotation1764
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1764/range1764/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_C0062/annotation1764/range1764/1
type: Range
persistent identity: range1764
display id: range1764
version: 1
top level: luxr
direction: direction/forward
start: 174
end: 174
orientation: inline

A
type: SequenceAnnotation
persistent identity: annotation1765
display id: annotation1765
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1765/range1765/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_C0062/annotation1765/range1765/1
type: Range
persistent identity: range1765
display id: range1765
version: 1
top level: luxr
direction: direction/forward
start: 492
end: 492
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213986
display id: annotation2213986
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation2213986/range2213986/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0062/annotation2213986/range2213986/1
type: Range
persistent identity: range2213986
display id: range2213986
version: 1
top level: luxr
direction: direction/forward
start: 757
end: 781
orientation: inline

https://synbiohub.org/public/igem/BBa_K233321_sequence/1
type: Sequence
persistent identity: BBa K233321 sequence
display id: BBa_K233321_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acctgtaggatcgtacaggttgacacaagaaaatggtttgttgatactcgaataaatactagagaaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacagctagctcagtcctaggtactgtgctagctactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggttgacacaagaaaatggtttgttgatactcgaataaatactagagtgtggaattgtgagcgctcacaattccacatactagagattaaagaggagaaatactagagaattcatcagtgatagtt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0261_sequence/1
type: Sequence
persistent identity: BBa C0261 sequence
display id: BBa_C0261_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0030_sequence/1
type: Sequence
persistent identity: BBa B0030 sequence
display id: BBa_B0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23102_sequence/1
type: Sequence
persistent identity: BBa J23102 sequence
display id: BBa_J23102_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctaggtactgtgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K124003_sequence/1
type: Sequence
persistent identity: BBa K124003 sequence
display id: BBa_K124003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattcatcagtgatagttacgttacgctgcgccttctttttttcccttccaatataagaactacgcaatccgttactggcggaggcgttgctatgaaatcaatggacaaaatctcaactggcattgcctacggaacatctgctggtagtgcgggatactggtttttgcagtggttggatcaggtcagtccgtcacagtgggctgcgattggagtgcttggaagccttgtgttgggttttctcacttatctgacaaatctgtatttcaaaatcagagaagacagacgaaaggctgcgagaggtgaataatgcctccatcattacgaaaagccgttgctgctgctattggtggcggagcaattgctatagcatcagtgttaatcactggcccaagtggtaacgatggtctggaaggtgtcagctacataccatacaaagatattgttggtgtatggactgtatgtcacgggcatacaggaaaagacatcatgctcggtaaaacgtataccaaagcagaatgcaaagccctcctgaataaagaccttgccacggtcgccagacaaattaacccgtacatcaaagtcgatataccggaaacaatgcgcggcgctctttactcattgctttacaacgtgggtgctggcaatttcagaacatcgacgcttcttcgtaaaataaaccagggcgatatcaaaggcgcatgtgatcagctacgtcgctggacatatgctggcggtaagcaatggaaaggtctcatgactcgtcgtgagattgagcgtgaaatctgtttgtggggtcagcaatgaacagagtaaccgcgattatctccgctctggttatctgcatcatcgtctgcctgtcatgggctgttaatcattaccgtgataacgccattacctacaaagcccagcgcgacaaaaatgccagagaactgacgctggcgaaccgggtaattactgacatacagatgcgtcagcgtgatgttgctgcgctcgatgcaaaatacacgaaggagttagctgatgcgaaagctgaaaatgatgctctgcgtgatgatgttgccgctggtcgtcgtcggttgcacatcaaagcagtctgtcagtcagtgcgtgaagccacgactgcctccggcgtggataatgcagcctccccccgactggcagacaccgctgaacgggattatttcaccctcagagagaggctgatcactatgcaaaaacaactggaaggaacccagaagtatattaatgagcagtgcagatagagctgcccat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J37033_sequence/1
type: Sequence
persistent identity: BBa J37033 sequence
display id: BBa_J37033_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K233004_sequence/1
type: Sequence
persistent identity: BBa K233004 sequence
display id: BBa_K233004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgtggaattgtgagcgctcacaattccaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R1062_sequence/1
type: Sequence
persistent identity: BBa R1062 sequence
display id: BBa_R1062_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acctgtaggatcgtacaggttgacacaagaaaatggtttgttgatactcgaataaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0061_sequence/1
type: Sequence
persistent identity: BBa C0061 sequence
display id: BBa_C0061_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0062_sequence/1
type: Sequence
persistent identity: BBa C0062 sequence
display id: BBa_C0062_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcac
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z