LOCUS       BBa_K233322             1535 bp    DNA     linear       05-Nov-2024
DEFINITION  AHL and Lactose Modulated Cell Lysis
ACCESSION   BBa_K233322
VERSION     BBa_K233322.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=LuxR/HSL
     promoter        42..48
                     /label=-10
     misc_feature    54..54
                     /label=start
     promoter        20..25
                     /label=-35
     misc_feature    1..56
                     /label=BBa_R1062
     promoter        1..56
                     /label=BBa_R1062
     promoter        65..94
                     /label=BBa_K233004
     RBS             103..117
                     /label=RBS-1\Strong
     RBS             110..114
                     /label=RBS
     misc_feature    103..117
                     /label=BBa_B0030
     RBS             103..117
                     /label=BBa_B0030
     misc_feature    126..1398
                     /label=BBa_K124003
     stem_loop       1418..1461
                     /label=stem_loop
     misc_feature    1407..1486
                     /label=BBa_B0010
     terminator      1407..1486
                     /label=BBa_B0010
     stem_loop       1502..1521
                     /label=T7 TE
     misc_feature    1528..1528
                     /label=stop
     polyA_site      1522..1535
                     /label=polya
     misc_feature    1495..1535
                     /label=BBa_B0012
     terminator      1495..1535
                     /label=BBa_B0012
ORIGIN
        1 acctgtagga tcgtacaggt tgacacaaga aaatggtttg ttgatactcg aataaatact
       61 agagtgtgga attgtgagcg ctcacaattc cacatactag agattaaaga ggagaaatac
      121 tagagaattc atcagtgata gttacgttac gctgcgcctt ctttttttcc cttccaatat
      181 aagaactacg caatccgtta ctggcggagg cgttgctatg aaatcaatgg acaaaatctc
      241 aactggcatt gcctacggaa catctgctgg tagtgcggga tactggtttt tgcagtggtt
      301 ggatcaggtc agtccgtcac agtgggctgc gattggagtg cttggaagcc ttgtgttggg
      361 ttttctcact tatctgacaa atctgtattt caaaatcaga gaagacagac gaaaggctgc
      421 gagaggtgaa taatgcctcc atcattacga aaagccgttg ctgctgctat tggtggcgga
      481 gcaattgcta tagcatcagt gttaatcact ggcccaagtg gtaacgatgg tctggaaggt
      541 gtcagctaca taccatacaa agatattgtt ggtgtatgga ctgtatgtca cgggcataca
      601 ggaaaagaca tcatgctcgg taaaacgtat accaaagcag aatgcaaagc cctcctgaat
      661 aaagaccttg ccacggtcgc cagacaaatt aacccgtaca tcaaagtcga tataccggaa
      721 acaatgcgcg gcgctcttta ctcattgctt tacaacgtgg gtgctggcaa tttcagaaca
      781 tcgacgcttc ttcgtaaaat aaaccagggc gatatcaaag gcgcatgtga tcagctacgt
      841 cgctggacat atgctggcgg taagcaatgg aaaggtctca tgactcgtcg tgagattgag
      901 cgtgaaatct gtttgtgggg tcagcaatga acagagtaac cgcgattatc tccgctctgg
      961 ttatctgcat catcgtctgc ctgtcatggg ctgttaatca ttaccgtgat aacgccatta
     1021 cctacaaagc ccagcgcgac aaaaatgcca gagaactgac gctggcgaac cgggtaatta
     1081 ctgacataca gatgcgtcag cgtgatgttg ctgcgctcga tgcaaaatac acgaaggagt
     1141 tagctgatgc gaaagctgaa aatgatgctc tgcgtgatga tgttgccgct ggtcgtcgtc
     1201 ggttgcacat caaagcagtc tgtcagtcag tgcgtgaagc cacgactgcc tccggcgtgg
     1261 ataatgcagc ctccccccga ctggcagaca ccgctgaacg ggattatttc accctcagag
     1321 agaggctgat cactatgcaa aaacaactgg aaggaaccca gaagtatatt aatgagcagt
     1381 gcagatagag ctgcccatta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa
     1441 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac
     1501 tggctcacct tcgggtgggc ctttctgcgt ttata
//