LOCUS       BBa_K233323              980 bp    DNA     linear       25-Nov-2024
DEFINITION  AHL and Lactose modulated Gfp production
ACCESSION   BBa_K233323
VERSION     BBa_K233323.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=LuxR/HSL
     promoter        42..48
                     /label=-10
     misc_feature    54..54
                     /label=start
     promoter        20..25
                     /label=-35
     misc_feature    1..56
                     /label=BBa_R1062
     promoter        1..56
                     /label=BBa_R1062
     promoter        65..94
                     /label=BBa_K233004
     RBS             103..117
                     /label=RBS-1\Strong
     RBS             110..114
                     /label=RBS
     misc_feature    103..117
                     /label=BBa_B0030
     RBS             103..117
                     /label=BBa_B0030
     CDS             124..843
                     /label=GFP protein
     CDS             124..843
                     /label=BBa_E0040
     stem_loop       863..906
                     /label=stem_loop
     misc_feature    852..931
                     /label=BBa_B0010
     terminator      852..931
                     /label=BBa_B0010
     stem_loop       947..966
                     /label=T7 TE
     misc_feature    973..973
                     /label=stop
     polyA_site      967..980
                     /label=polya
     misc_feature    940..980
                     /label=BBa_B0012
     terminator      940..980
                     /label=BBa_B0012
ORIGIN
        1 acctgtagga tcgtacaggt tgacacaaga aaatggtttg ttgatactcg aataaatact
       61 agagtgtgga attgtgagcg ctcacaattc cacatactag agattaaaga ggagaaatac
      121 tagatgcgta aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat
      181 ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga tgcaacatac
      241 ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc atggccaaca
      301 cttgtcacta ctttcggtta tggtgttcaa tgctttgcga gatacccaga tcatatgaaa
      361 cagcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaaag aactatattt
      421 ttcaaagatg acgggaacta caagacacgt gctgaagtca agtttgaagg tgataccctt
      481 gttaatagaa tcgagttaaa aggtattgat tttaaagaag atggaaacat tcttggacac
      541 aaattggaat acaactataa ctcacacaat gtatacatca tggcagacaa acaaaagaat
      601 ggaatcaaag ttaacttcaa aattagacac aacattgaag atggaagcgt tcaactagca
      661 gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat
      721 tacctgtcca cacaatctgc cctttcgaaa gatcccaacg aaaagagaga ccacatggtc
      781 cttcttgagt ttgtaacagc tgctgggatt acacatggca tggatgaact atacaaataa
      841 taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
      901 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg
      961 tgggcctttc tgcgtttata
//