LOCUS BBa_K233325 6371 bp DNA linear 07-May-2024 DEFINITION The complete turing machine construct ACCESSION BBa_K233325 VERSION BBa_K233325.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23102 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 misc_feature 62..63 /label=prefix misc_feature 235..235 /label=T misc_feature 553..553 /label=A CDS 62..811 /label=luxR misc_feature 62..817 /label=BBa_C0062 misc_feature 818..842 /label=Help:Barcodes CDS 62..817 /label=BBa_C0062 misc_feature 44..842 /label=BBa_J37033 misc_feature 1938..1976 /label=LVA CDS 849..1976 /label=lacI-LVA misc_feature 849..1976 /label=BBa_C0012 misc_feature 1977..2001 /label=Help:Barcodes CDS 849..1976 /label=BBa_C0012 stem_loop 2021..2064 /label=stem_loop misc_feature 2010..2089 /label=BBa_B0010 terminator 2010..2089 /label=BBa_B0010 stem_loop 2105..2124 /label=T7 TE misc_feature 2131..2131 /label=stop polyA_site 2125..2138 /label=polya misc_feature 2098..2138 /label=BBa_B0012 terminator 2098..2138 /label=BBa_B0012 terminator 2010..2138 /label=BBa_B0015 misc_feature 2147..2243 /label=BBa_R0065 misc_feature 2152..2171 /label=Lux Box promoter 2193..2198 /label=-10 misc_feature 2203..2219 /label=OR2 cI promoter 2204..2204 /label=lux p(R) start promoter 2217..2222 /label=-35 misc_feature 2227..2243 /label=OR1 cI promoter 2240..2243 /label=-10 promoter 2147..2243 /label=BBa_R0065 misc_feature 2252..2263 /label=B0034 misc_feature 2270..2270 /label=P2 ogr CDS 2270..2488 /label=P2 ogr misc_feature 2252..2488 /label=BBa_I746350 stem_loop 2508..2551 /label=stem_loop misc_feature 2497..2576 /label=BBa_B0010 terminator 2497..2576 /label=BBa_B0010 stem_loop 2592..2611 /label=T7 TE misc_feature 2618..2618 /label=stop polyA_site 2612..2625 /label=polya misc_feature 2585..2625 /label=BBa_B0012 terminator 2585..2625 /label=BBa_B0012 terminator 2497..2625 /label=BBa_B0015 promoter 2634..2725 /label=PLL promoter 2634..2725 /label=BBa_I746365 misc_feature 2734..2745 /label=B0034 misc_feature 2752..2752 /label=P2 ogr CDS 2752..2970 /label=P2 ogr misc_feature 2734..2970 /label=BBa_I746350 CDS 2980..3687 /label=cI lambda misc_feature 3688..3720 /label=LVA misc_feature 3727..3751 /label=Help:Barcodes CDS 2977..3751 /label=BBa_C0051 stem_loop 3771..3814 /label=stem_loop misc_feature 3760..3839 /label=BBa_B0010 terminator 3760..3839 /label=BBa_B0010 stem_loop 3855..3874 /label=T7 TE misc_feature 3881..3881 /label=stop polyA_site 3875..3888 /label=polya misc_feature 3848..3888 /label=BBa_B0012 terminator 3848..3888 /label=BBa_B0012 terminator 3760..3888 /label=BBa_B0015 promoter 3897..3988 /label=PLL promoter 3897..3988 /label=BBa_I746365 promoter 3997..4026 /label=BBa_K233004 misc_feature 4039..4042 /label=conserved RBS 4035..4046 /label=BBa_B0034 misc_feature 4632..4663 /label=LVA CDS 4053..4631 /label=luxI misc_feature 4053..4670 /label=BBa_C0061 misc_feature 4671..4695 /label=Help:Barcodes CDS 4053..4670 /label=BBa_C0061 stem_loop 4715..4758 /label=stem_loop misc_feature 4704..4783 /label=BBa_B0010 terminator 4704..4783 /label=BBa_B0010 stem_loop 4799..4818 /label=T7 TE misc_feature 4825..4825 /label=stop polyA_site 4819..4832 /label=polya misc_feature 4792..4832 /label=BBa_B0012 terminator 4792..4832 /label=BBa_B0012 terminator 4704..4832 /label=BBa_B0015 misc_feature 4841..4860 /label=LuxR/HSL promoter 4860..4865 /label=-35 promoter 4882..4887 /label=-10 misc_feature 4893..4893 /label=start misc_feature 4841..4895 /label=BBa_R0062 promoter 4841..4895 /label=BBa_R0062 promoter 4904..4933 /label=BBa_K233004 misc_feature 4946..4949 /label=conserved RBS 4942..4953 /label=BBa_B0034 misc_feature 4962..6234 /label=BBa_K124003 stem_loop 6254..6297 /label=stem_loop misc_feature 6243..6322 /label=BBa_B0010 terminator 6243..6322 /label=BBa_B0010 stem_loop 6338..6357 /label=T7 TE misc_feature 6364..6364 /label=stop polyA_site 6358..6371 /label=polya misc_feature 6331..6371 /label=BBa_B0012 terminator 6331..6371 /label=BBa_B0012 terminator 6243..6371 /label=BBa_B0015 ORIGIN 1 ttgacagcta gctcagtcct aggtactgtg ctagctacta gagaaagagg agaaatacta 61 gatgaaaaac ataaatgccg acgacacata cagaataatt aataaaatta aagcttgtag 121 aagcaataat gatattaatc aatgcttatc tgatatgact aaaatggtac attgtgaata 181 ttatttactc gcgatcattt atcctcattc tatggttaaa tctgatattt caatcctaga 241 taattaccct aaaaaatgga ggcaatatta tgatgacgct aatttaataa aatatgatcc 301 tatagtagat tattctaact ccaatcattc accaattaat tggaatatat ttgaaaacaa 361 tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg aaaacatcag gtcttatcac 421 tgggtttagt ttccctattc atacggctaa caatggcttc ggaatgctta gttttgcaca 481 ttcagaaaaa gacaactata tagatagttt atttttacat gcgtgtatga acataccatt 541 aattgttcct tctctagttg ataattatcg aaaaataaat atagcaaata ataaatcaaa 601 caacgattta accaaaagag aaaaagaatg tttagcgtgg gcatgcgaag gaaaaagctc 661 ttgggatatt tcaaaaatat taggttgcag tgagcgtact gtcactttcc atttaaccaa 721 tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt atttctaaag caattttaac 781 aggagcaatt gattgcccat actttaaaaa ttaataacac tgatagtgct agtgtagatc 841 actactagat ggtgaatgtg aaaccagtaa cgttatacga tgtcgcagag tatgccggtg 901 tctcttatca gaccgtttcc cgcgtggtga accaggccag ccacgtttct gcgaaaacgc 961 gggaaaaagt ggaagcggcg atggcggagc tgaattacat tcccaaccgc gtggcacaac 1021 aactggcggg caaacagtcg ttgctgattg gcgttgccac ctccagtctg gccctgcacg 1081 cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt gccagcgtgg 1141 tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg cacaatcttc 1201 tcgcgcaacg cgtcagtggg ctgatcatta actatccgct ggatgaccag gatgccattg 1261 ctgtggaagc tgcctgcact aatgttccgg cgttatttct tgatgtctct gaccagacac 1321 ccatcaacag tattattttc tcccatgaag acggtacgcg actgggcgtg gagcatctgg 1381 tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc attaagttct gtctcggcgc 1441 gtctgcgtct ggctggctgg cataaatatc tcactcgcaa tcaaattcag ccgatagcgg 1501 aacgggaagg cgactggagt gccatgtccg gttttcaaca aaccatgcaa atgctgaatg 1561 agggcatcgt tcccactgcg atgctggttg ccaacgatca gatggcgctg ggcgcaatgc 1621 gcgccattac cgagtccggg ctgcgcgttg gtgcggatat ctcggtagtg ggatacgacg 1681 ataccgaaga cagctcatgt tatatcccgc cgttaaccac catcaaacag gattttcgcc 1741 tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag gcggtgaagg 1801 gcaatcagct gttgcccgtc tcactggtga aaagaaaaac caccctggcg cccaatacgc 1861 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc 1921 gactggaaag cgggcaggct gcaaacgacg aaaactacgc tttagtagct taataactct 1981 gatagtgcta gtgtagatct ctactagagc caggcatcaa ataaaacgaa aggctcagtc 2041 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 2101 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagtaag cacctgtagg 2161 atcgtacagg tttacgcaag aaaatggttt gttatagtcg aataacaccg tgcgtgttga 2221 ctattttacc tctggcggtg atatactaga gaaagaggag aaatactaga tgtttcattg 2281 tcctttatgc cagcatgccg cacatgcgcg tacaagtcgc tatatcactg acacgacaaa 2341 agagcgttat catcagtgcc agaacgtgaa ttgcagcgcc acgttcatca cttatgagtc 2401 ggtacagcga tacatcgtga agccgggaga agtccacgcc gtaaggccgc acccgttgcc 2461 atcagggcag caaattatgt ggatgtaata ctagagccag gcatcaaata aaacgaaagg 2521 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact 2581 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta gaggtggaga 2641 tgaacaaaaa acacacagcc attgtaagac agcctgaaca aatcccccct gttgcgtctg 2701 ctgaaaatat tcacaaaata aagcgtacta gagaaagagg agaaatacta gatgtttcat 2761 tgtcctttat gccagcatgc cgcacatgcg cgtacaagtc gctatatcac tgacacgaca 2821 aaagagcgtt atcatcagtg ccagaacgtg aattgcagcg ccacgttcat cacttatgag 2881 tcggtacagc gatacatcgt gaagccggga gaagtccacg ccgtaaggcc gcacccgttg 2941 ccatcagggc agcaaattat gtggatgtaa tactagatga gcacaaaaaa gaaaccatta 3001 acacaagagc agcttgagga cgcacgtcgc cttaaagcaa tttatgaaaa aaagaaaaat 3061 gaacttggct tatcccagga atctgtcgca gacaagatgg ggatggggca gtcaggcgtt 3121 ggtgctttat ttaatggcat caatgcatta aatgcttata acgccgcatt gcttgcaaaa 3181 attctcaaag ttagcgttga agaatttagc ccttcaatcg ccagagaaat ctacgagatg 3241 tatgaagcgg ttagtatgca gccgtcactt agaagtgagt atgagtaccc tgttttttct 3301 catgttcagg cagggatgtt ctcacctgag cttagaacct ttaccaaagg tgatgcggag 3361 agatgggtaa gcacaaccaa aaaagccagt gattctgcat tctggcttga ggttgaaggt 3421 aattccatga ccgcaccaac aggctccaag ccaagctttc ctgacggaat gttaattctc 3481 gttgaccctg agcaggctgt tgagccaggt gatttctgca tagccagact tgggggtgat 3541 gagtttacct tcaagaaact gatcagggat agcggtcagg tgtttttaca accactaaac 3601 ccacagtacc caatgatccc atgcaatgag agttgttccg ttgtggggaa agttatcgct 3661 agtcagtggc ctgaagagac gtttggcgct gcaaacgacg aaaactacgc tttagtagct 3721 taataacgct gatagtgcta gtgtagatcg ctactagagc caggcatcaa ataaaacgaa 3781 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct 3841 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata ctagaggtgg 3901 agatgaacaa aaaacacaca gccattgtaa gacagcctga acaaatcccc cctgttgcgt 3961 ctgctgaaaa tattcacaaa ataaagcgta ctagagtgtg gaattgtgag cgctcacaat 4021 tccacatact agagaaagag gagaaatact agatgactat aatgataaaa aaatcggatt 4081 ttttggcaat tccatcggag gagtataaag gtattctaag tcttcgttat caagtgttta 4141 agcaaagact tgagtgggac ttagttgtag aaaataacct tgaatcagat gagtatgata 4201 actcaaatgc agaatatatt tatgcttgtg atgatactga aaatgtaagt ggatgctggc 4261 gtttattacc tacaacaggt gattatatgc tgaaaagtgt ttttcctgaa ttgcttggtc 4321 aacagagtgc tcccaaagat cctaatatag tcgaattaag tcgttttgct gtaggtaaaa 4381 atagctcaaa gataaataac tctgctagtg aaattacaat gaaactattt gaagctatat 4441 ataaacacgc tgttagtcaa ggtattacag aatatgtaac agtaacatca acagcaatag 4501 agcgattttt aaagcgtatt aaagttcctt gtcatcgtat tggagacaaa gaaattcatg 4561 tattaggtga tactaaatcg gttgtattgt ctatgcctat taatgaacag tttaaaaaag 4621 cagtcttaaa tgctgcaaac gacgaaaact acgctttagt agcttaataa ctctgatagt 4681 gctagtgtag atctctacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga 4741 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg 4801 ctcaccttcg ggtgggcctt tctgcgttta tatactagag acctgtagga tcgtacaggt 4861 ttacgcaaga aaatggtttg ttatagtcga ataaatacta gagtgtggaa ttgtgagcgc 4921 tcacaattcc acatactaga gaaagaggag aaatactaga gaattcatca gtgatagtta 4981 cgttacgctg cgccttcttt ttttcccttc caatataaga actacgcaat ccgttactgg 5041 cggaggcgtt gctatgaaat caatggacaa aatctcaact ggcattgcct acggaacatc 5101 tgctggtagt gcgggatact ggtttttgca gtggttggat caggtcagtc cgtcacagtg 5161 ggctgcgatt ggagtgcttg gaagccttgt gttgggtttt ctcacttatc tgacaaatct 5221 gtatttcaaa atcagagaag acagacgaaa ggctgcgaga ggtgaataat gcctccatca 5281 ttacgaaaag ccgttgctgc tgctattggt ggcggagcaa ttgctatagc atcagtgtta 5341 atcactggcc caagtggtaa cgatggtctg gaaggtgtca gctacatacc atacaaagat 5401 attgttggtg tatggactgt atgtcacggg catacaggaa aagacatcat gctcggtaaa 5461 acgtatacca aagcagaatg caaagccctc ctgaataaag accttgccac ggtcgccaga 5521 caaattaacc cgtacatcaa agtcgatata ccggaaacaa tgcgcggcgc tctttactca 5581 ttgctttaca acgtgggtgc tggcaatttc agaacatcga cgcttcttcg taaaataaac 5641 cagggcgata tcaaaggcgc atgtgatcag ctacgtcgct ggacatatgc tggcggtaag 5701 caatggaaag gtctcatgac tcgtcgtgag attgagcgtg aaatctgttt gtggggtcag 5761 caatgaacag agtaaccgcg attatctccg ctctggttat ctgcatcatc gtctgcctgt 5821 catgggctgt taatcattac cgtgataacg ccattaccta caaagcccag cgcgacaaaa 5881 atgccagaga actgacgctg gcgaaccggg taattactga catacagatg cgtcagcgtg 5941 atgttgctgc gctcgatgca aaatacacga aggagttagc tgatgcgaaa gctgaaaatg 6001 atgctctgcg tgatgatgtt gccgctggtc gtcgtcggtt gcacatcaaa gcagtctgtc 6061 agtcagtgcg tgaagccacg actgcctccg gcgtggataa tgcagcctcc ccccgactgg 6121 cagacaccgc tgaacgggat tatttcaccc tcagagagag gctgatcact atgcaaaaac 6181 aactggaagg aacccagaag tatattaatg agcagtgcag atagagctgc ccattactag 6241 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg 6301 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt 6361 ctgcgtttat a //