##gff-version 3
##sequence-region BBa_K233325 1 6371
BBa_K233325	.	promoter	4904	4933	.	+	0	ID=BBa_K233004;Name=BBa_K233004
BBa_K233325	.	promoter	3897	3988	.	+	0	ID=BBa_I746365;Name=BBa_I746365
BBa_K233325	.	promoter	3897	3988	.	+	0	ID=annotation1943788;Name=PLL;Parent=BBa_I746365
BBa_K233325	.	terminator	2010	2138	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K233325	.	terminator	2010	2089	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K233325	.	engineered_region	2010	2089	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K233325	.	stem_loop	2021	2064	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K233325	.	terminator	2098	2138	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K233325	.	stop_codon	2131	2131	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K233325	.	polyA_site	2125	2138	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K233325	.	stem_loop	2105	2124	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K233325	.	engineered_region	2098	2138	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K233325	.	terminator	3760	3888	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K233325	.	terminator	3760	3839	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K233325	.	engineered_region	3760	3839	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K233325	.	stem_loop	3771	3814	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K233325	.	terminator	3848	3888	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K233325	.	stop_codon	3881	3881	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K233325	.	polyA_site	3875	3888	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K233325	.	stem_loop	3855	3874	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K233325	.	engineered_region	3848	3888	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K233325	.	promoter	2147	2243	.	+	0	ID=BBa_R0065;Name=BBa_R0065
BBa_K233325	.	promoter	2217	2222	.	+	0	ID=annotation1986779;Name=-35;Parent=BBa_R0065
BBa_K233325	.	non_covalent_binding_site	2203	2219	.	+	0	ID=annotation1986777;Name=OR2 cI;Parent=BBa_R0065
BBa_K233325	.	promoter	2204	2204	.	+	0	ID=annotation1986778;Name=lux p(R) start;Parent=BBa_R0065
BBa_K233325	.	non_covalent_binding_site	2152	2171	.	+	0	ID=annotation1986775;Name=Lux Box;Parent=BBa_R0065
BBa_K233325	.	engineered_region	2147	2243	.	+	0	ID=annotation1986774;Name=BBa_R0065;Parent=BBa_R0065
BBa_K233325	.	non_covalent_binding_site	2227	2243	.	+	0	ID=annotation1986780;Name=OR1 cI;Parent=BBa_R0065
BBa_K233325	.	promoter	2193	2198	.	+	0	ID=annotation1986776;Name=-10;Parent=BBa_R0065
BBa_K233325	.	promoter	2240	2243	.	+	0	ID=annotation1986781;Name=-10;Parent=BBa_R0065
BBa_K233325	.	engineered_region	2734	2970	.	+	0	ID=BBa_I746350;Name=BBa_I746350
BBa_K233325	.	start_codon	2752	2752	.	+	0	ID=annotation1943866;Name=P2 ogr;Parent=BBa_I746350
BBa_K233325	.	CDS	2752	2970	.	+	0	ID=annotation1943867;Name=P2 ogr;Parent=BBa_I746350
BBa_K233325	.	engineered_region	2734	2745	.	+	0	ID=annotation1943865;Name=B0034;Parent=BBa_I746350
BBa_K233325	.	engineered_region	2252	2488	.	+	0	ID=BBa_I746350;Name=BBa_I746350
BBa_K233325	.	start_codon	2270	2270	.	+	0	ID=annotation1943866;Name=P2 ogr;Parent=BBa_I746350
BBa_K233325	.	CDS	2270	2488	.	+	0	ID=annotation1943867;Name=P2 ogr;Parent=BBa_I746350
BBa_K233325	.	engineered_region	2252	2263	.	+	0	ID=annotation1943865;Name=B0034;Parent=BBa_I746350
BBa_K233325	.	ribosome_entry_site	4035	4046	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K233325	.	conserved_region	4039	4042	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K233325	.	CDS	2977	3751	.	+	0	ID=BBa_C0051;Name=BBa_C0051
BBa_K233325	.	engineered_tag	3727	3751	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_K233325	.	CDS	2980	3687	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_K233325	.	tag	3688	3720	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_K233325	.	ribosome_entry_site	4942	4953	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K233325	.	conserved_region	4946	4949	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K233325	.	terminator	4704	4832	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K233325	.	terminator	4704	4783	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K233325	.	engineered_region	4704	4783	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K233325	.	stem_loop	4715	4758	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K233325	.	terminator	4792	4832	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K233325	.	stop_codon	4825	4825	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K233325	.	polyA_site	4819	4832	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K233325	.	stem_loop	4799	4818	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K233325	.	engineered_region	4792	4832	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K233325	.	engineered_region	44	842	.	+	0	ID=BBa_J37033;Name=BBa_J37033
BBa_K233325	.	ribosome_entry_site	44	55	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J37033
BBa_K233325	.	conserved_region	48	51	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K233325	.	CDS	62	817	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_J37033
BBa_K233325	.	engineered_tag	818	842	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K233325	.	engineered_region	62	817	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K233325	.	engineered_region	62	63	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K233325	.	point_mutation	235	235	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K233325	.	CDS	62	811	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K233325	.	point_mutation	553	553	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K233325	.	promoter	2634	2725	.	+	0	ID=BBa_I746365;Name=BBa_I746365
BBa_K233325	.	promoter	2634	2725	.	+	0	ID=annotation1943788;Name=PLL;Parent=BBa_I746365
BBa_K233325	.	promoter	3997	4026	.	+	0	ID=BBa_K233004;Name=BBa_K233004
BBa_K233325	.	sequence_feature	4962	6234	.	+	0	ID=BBa_K124003;Name=BBa_K124003
BBa_K233325	.	CDS	4053	4670	.	+	0	ID=BBa_C0061;Name=BBa_C0061
BBa_K233325	.	CDS	4053	4631	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K233325	.	engineered_tag	4671	4695	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K233325	.	engineered_region	4053	4670	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K233325	.	tag	4632	4663	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K233325	.	terminator	6243	6371	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K233325	.	terminator	6243	6322	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K233325	.	engineered_region	6243	6322	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K233325	.	stem_loop	6254	6297	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K233325	.	terminator	6331	6371	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K233325	.	stop_codon	6364	6364	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K233325	.	polyA_site	6358	6371	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K233325	.	stem_loop	6338	6357	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K233325	.	engineered_region	6331	6371	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K233325	.	promoter	1	35	.	+	0	ID=BBa_J23102;Name=BBa_J23102
BBa_K233325	.	CDS	849	1976	.	+	0	ID=BBa_C0012;Name=BBa_C0012
BBa_K233325	.	engineered_tag	1977	2001	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_K233325	.	engineered_region	849	1976	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_K233325	.	tag	1938	1976	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_K233325	.	CDS	849	1976	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_K233325	.	terminator	2497	2625	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K233325	.	terminator	2497	2576	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K233325	.	engineered_region	2497	2576	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K233325	.	stem_loop	2508	2551	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K233325	.	terminator	2585	2625	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K233325	.	stop_codon	2618	2618	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K233325	.	polyA_site	2612	2625	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K233325	.	stem_loop	2592	2611	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K233325	.	engineered_region	2585	2625	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K233325	.	promoter	4841	4895	.	+	0	ID=BBa_R0062;Name=BBa_R0062
BBa_K233325	.	start_codon	4893	4893	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K233325	.	promoter	4882	4887	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K233325	.	promoter	4860	4865	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K233325	.	engineered_region	4841	4895	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K233325	.	non_covalent_binding_site	4841	4860	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
>BBa_K233325
ttgacagctagctcagtcctaggtactgtgctagctactagagaaagaggagaaatactagatgaaaaacataaatgcc
gacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatga
ctaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcct
agataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattct
aactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaag
aagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttt
tgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttct
ctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtt
tagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttcca
tttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaatt
gattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagatggtgaatgtgaaaccagta
acgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgttt
ctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggc
gggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgatt
aaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcgg
cggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgt
ggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcc
catgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccat
taagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcgga
acgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcg
atgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggata
tctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcg
cctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgccc
gtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaa
tgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaata
actctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcct
ttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgt
ttatatactagagtaagcacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataacaccgtg
cgtgttgactattttacctctggcggtgatatactagagaaagaggagaaatactagatgtttcattgtcctttatgcc
agcatgccgcacatgcgcgtacaagtcgctatatcactgacacgacaaaagagcgttatcatcagtgccagaacgtgaa
ttgcagcgccacgttcatcacttatgagtcggtacagcgatacatcgtgaagccgggagaagtccacgccgtaaggccg
cacccgttgccatcagggcagcaaattatgtggatgtaatactagagccaggcatcaaataaaacgaaaggctcagtcg
aaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtg
ggcctttctgcgtttatatactagaggtggagatgaacaaaaaacacacagccattgtaagacagcctgaacaaatccc
ccctgttgcgtctgctgaaaatattcacaaaataaagcgtactagagaaagaggagaaatactagatgtttcattgtcc
tttatgccagcatgccgcacatgcgcgtacaagtcgctatatcactgacacgacaaaagagcgttatcatcagtgccag
aacgtgaattgcagcgccacgttcatcacttatgagtcggtacagcgatacatcgtgaagccgggagaagtccacgccg
taaggccgcacccgttgccatcagggcagcaaattatgtggatgtaatactagatgagcacaaaaaagaaaccattaac
acaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaa
tctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttata
acgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagat
gtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatg
ttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctg
cattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaat
tctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaag
aaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagtt
gttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgcttt
agtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcga
aagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgg
gcctttctgcgtttatatactagaggtggagatgaacaaaaaacacacagccattgtaagacagcctgaacaaatcccc
cctgttgcgtctgctgaaaatattcacaaaataaagcgtactagagtgtggaattgtgagcgctcacaattccacatac
tagagaaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataa
aggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatca
gatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttat
tacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaa
tatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaa
ctatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagc
gatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggt
tgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagta
gcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaaga
ctgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcct
ttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatac
tagagtgtggaattgtgagcgctcacaattccacatactagagaaagaggagaaatactagagaattcatcagtgatag
ttacgttacgctgcgccttctttttttcccttccaatataagaactacgcaatccgttactggcggaggcgttgctatg
aaatcaatggacaaaatctcaactggcattgcctacggaacatctgctggtagtgcgggatactggtttttgcagtggt
tggatcaggtcagtccgtcacagtgggctgcgattggagtgcttggaagccttgtgttgggttttctcacttatctgac
aaatctgtatttcaaaatcagagaagacagacgaaaggctgcgagaggtgaataatgcctccatcattacgaaaagccg
ttgctgctgctattggtggcggagcaattgctatagcatcagtgttaatcactggcccaagtggtaacgatggtctgga
aggtgtcagctacataccatacaaagatattgttggtgtatggactgtatgtcacgggcatacaggaaaagacatcatg
ctcggtaaaacgtataccaaagcagaatgcaaagccctcctgaataaagaccttgccacggtcgccagacaaattaacc
cgtacatcaaagtcgatataccggaaacaatgcgcggcgctctttactcattgctttacaacgtgggtgctggcaattt
cagaacatcgacgcttcttcgtaaaataaaccagggcgatatcaaaggcgcatgtgatcagctacgtcgctggacatat
gctggcggtaagcaatggaaaggtctcatgactcgtcgtgagattgagcgtgaaatctgtttgtggggtcagcaatgaa
cagagtaaccgcgattatctccgctctggttatctgcatcatcgtctgcctgtcatgggctgttaatcattaccgtgat
aacgccattacctacaaagcccagcgcgacaaaaatgccagagaactgacgctggcgaaccgggtaattactgacatac
agatgcgtcagcgtgatgttgctgcgctcgatgcaaaatacacgaaggagttagctgatgcgaaagctgaaaatgatgc
tctgcgtgatgatgttgccgctggtcgtcgtcggttgcacatcaaagcagtctgtcagtcagtgcgtgaagccacgact
gcctccggcgtggataatgcagcctccccccgactggcagacaccgctgaacgggattatttcaccctcagagagaggc
tgatcactatgcaaaaacaactggaaggaacccagaagtatattaatgagcagtgcagatagagctgcccattactaga
gccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctc
tctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata