BBa_K234093
1
BBa_K234093
Gal1 + Kozak + GFP +Stop +CYC1 Terminator
2009-09-15T11:00:00Z
2015-05-08T01:11:35Z
Composed from:
BBa_J63006 in BBa_J63010 [GAL1 + Kozak]
BBa_K234091 in pSB1A3 [GFP Silver standard]
BBa_K234092 in pSB1A3 [stop + CYC1 Terminator]
in that order
This part will constitutively express GFP in yeast.
false
false
_331_
0
2809
9
Not in stock
false
None
false
Alex Power and Daniel Jedrysiak
component2021311
1
BBa_K234091
component2021309
1
BBa_J63006
component2021314
1
BBa_K234092
annotation2021314
1
BBa_K234092
range2021314
1
1275
1495
annotation2021309
1
BBa_J63006
range2021309
1
1
549
annotation2021311
1
BBa_K234091
range2021311
1
558
1266
BBa_K234092
1
BBa_K234092
Stop codon + CYC1 Terminator
2009-07-21T11:00:00Z
2015-05-08T01:11:35Z
This part was PCR amplified from the pRS4D1T123 plasmid generously donated to us by Dr. James J. Collins
Ref:
Kevin F. Murphy, G??bor Bal??zsi, and James J. Collins. Combinatorial promoter design for engineering noisy gene expression. Proc Natl Acad Sci U S A. 2007 July 31; 104(31): 12726???12731.
This part contains a stop codon followed by the native yeast CYC1 terminator, this part is in the Silver standard format.
false
false
_331_
0
2809
9
It's complicated
false
No special design considerations.
false
Daniel Jedrysiak
annotation2012247
1
stop codon
range2012247
1
1
3
annotation2012248
1
CYC1 Terminator
range2012248
1
4
221
BBa_J63006
1
GAL1
yeast GAL1 promoter
2006-10-10T11:00:00Z
2015-08-31T01:56:26Z
genomic GAL1 locus
Released HQ 2013
GAL1 promoter from S. cerevisiae
false
true
_97_
0
545
97
In stock
false
unchanged from genomic sequence
true
Caroline Ajo-Franklin
BBa_K234091
1
BBa_K234091
GFP, Silver standard
2009-07-21T11:00:00Z
2015-05-08T01:11:35Z
This part was PCR amplified out of the pRS4D1T123 plasmid which was generously given to us by Dr. James J. Collins
Ref:
Kevin F. Murphy, G??bor Bal??zsi, and James J. Collins. Combinatorial promoter design for engineering noisy gene expression. PNAS 104(31), 12726???12731 (2007).
This is a GFP reporter in the Silver standard (Assembly standard 23)format. It does not contain a start or stop codon.
false
false
_331_
0
2809
9
Not in stock
false
No special design considerations.
false
Daniel Jedrysiak
annotation2012246
1
GFP
range2012246
1
1
709
BBa_J63006_sequence
1
ccccattatcttagcctaaaaaaaccttctctttggaactttcagtaatacgcttaactgctcattgctatattgaagtacggattagaagccgccgagcgggtgacagccctccgaaggaagactctcctccgtgcgtcctcgtcttcaccggtcgcgttcctgaaacgcagatgtgcctcgcgccgcactgctccgaacaataaagattctacaatactagcttttatggttatgaagaggaaaaattggcagtaacctggccccacaaaccttcaaatgaacgaatcaaattaacaaccataggatgataatgcgattagttttttagccttatttctggggtaattaatcagcgaagcgatgatttttgatctattaacagatatataaatgcaaaaactgcataaccactttaactaatactttcaacattttcggtttgtattacttcttattcaaatgtaataaaagtatcaacaaaaaattgttaatatacctctatactttaacgtcaaggaggaaactagacccgccgccaccatggag
BBa_K234091_sequence
1
taaaggtgaagaattattcactggtgttgtcccaattttggttgaattagatggtgatgttaatggtcacaaattttctgtctccggtgaaggtgaaggtgatgctacttacggtaaattgaccttaaaatttatttgtactactggtaaattgccagttccatggccaaccttagtcactactttcggttatggtgttcaatgttttgctagatacccagatcatatgaaacaacatgactttttcaagtctgccatgccagaaggttatgttcaagaaagaactatttttttcaaagatgacggtaactacaagaccagagctgaagtcaagtttgaaggtgataccttagttaatagaatcgaattaaaaggtattgattttaaagaagatggtaacattttaggtcacaaattggaatacaactataactctcacaatgtttacatcatggctgacaaacaaaagaatggtatcaaagttaacttcaaaattagacacaacattgaagatggttctgttcaattagctgaccattatcaacaaaatactccaattggtgatggtccagtcttgttaccagacaaccattacttatccactcaatctgccttatccaaagatccaaacgaaaagagagaccacatggtcttgttagaatttgttactgctgctggtattacccatggtatggatgaattgtacaaa
BBa_K234092_sequence
1
taagatccgctctaaccgaaaaggaaggagttagacaacctgaagtctaggtccctatttatttttttatagttatgttagtattaagaacgttatttatatttcaaatttttcttttttttctgtacagacgcgtgtacgcatgtaacattatactgaaaaccttgcttgagaaggttttgggacgctcgaagatccagctgcattaatgaatcggccaa
BBa_K234093_sequence
1
ccccattatcttagcctaaaaaaaccttctctttggaactttcagtaatacgcttaactgctcattgctatattgaagtacggattagaagccgccgagcgggtgacagccctccgaaggaagactctcctccgtgcgtcctcgtcttcaccggtcgcgttcctgaaacgcagatgtgcctcgcgccgcactgctccgaacaataaagattctacaatactagcttttatggttatgaagaggaaaaattggcagtaacctggccccacaaaccttcaaatgaacgaatcaaattaacaaccataggatgataatgcgattagttttttagccttatttctggggtaattaatcagcgaagcgatgatttttgatctattaacagatatataaatgcaaaaactgcataaccactttaactaatactttcaacattttcggtttgtattacttcttattcaaatgtaataaaagtatcaacaaaaaattgttaatatacctctatactttaacgtcaaggaggaaactagacccgccgccaccatggagtactagagtaaaggtgaagaattattcactggtgttgtcccaattttggttgaattagatggtgatgttaatggtcacaaattttctgtctccggtgaaggtgaaggtgatgctacttacggtaaattgaccttaaaatttatttgtactactggtaaattgccagttccatggccaaccttagtcactactttcggttatggtgttcaatgttttgctagatacccagatcatatgaaacaacatgactttttcaagtctgccatgccagaaggttatgttcaagaaagaactatttttttcaaagatgacggtaactacaagaccagagctgaagtcaagtttgaaggtgataccttagttaatagaatcgaattaaaaggtattgattttaaagaagatggtaacattttaggtcacaaattggaatacaactataactctcacaatgtttacatcatggctgacaaacaaaagaatggtatcaaagttaacttcaaaattagacacaacattgaagatggttctgttcaattagctgaccattatcaacaaaatactccaattggtgatggtccagtcttgttaccagacaaccattacttatccactcaatctgccttatccaaagatccaaacgaaaagagagaccacatggtcttgttagaatttgttactgctgctggtattacccatggtatggatgaattgtacaaatactagagtaagatccgctctaaccgaaaaggaaggagttagacaacctgaagtctaggtccctatttatttttttatagttatgttagtattaagaacgttatttatatttcaaatttttcttttttttctgtacagacgcgtgtacgcatgtaacattatactgaaaaccttgcttgagaaggttttgggacgctcgaagatccagctgcattaatgaatcggccaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z