BBa_K234093 1 BBa_K234093 Gal1 + Kozak + GFP +Stop +CYC1 Terminator 2009-09-15T11:00:00Z 2015-05-08T01:11:35Z Composed from: BBa_J63006 in BBa_J63010 [GAL1 + Kozak] BBa_K234091 in pSB1A3 [GFP Silver standard] BBa_K234092 in pSB1A3 [stop + CYC1 Terminator] in that order This part will constitutively express GFP in yeast. false false _331_ 0 2809 9 Not in stock false None false Alex Power and Daniel Jedrysiak component2021311 1 BBa_K234091 component2021309 1 BBa_J63006 component2021314 1 BBa_K234092 annotation2021314 1 BBa_K234092 range2021314 1 1275 1495 annotation2021309 1 BBa_J63006 range2021309 1 1 549 annotation2021311 1 BBa_K234091 range2021311 1 558 1266 BBa_K234092 1 BBa_K234092 Stop codon + CYC1 Terminator 2009-07-21T11:00:00Z 2015-05-08T01:11:35Z This part was PCR amplified from the pRS4D1T123 plasmid generously donated to us by Dr. James J. Collins Ref: Kevin F. Murphy, G??bor Bal??zsi, and James J. Collins. Combinatorial promoter design for engineering noisy gene expression. Proc Natl Acad Sci U S A. 2007 July 31; 104(31): 12726???12731. This part contains a stop codon followed by the native yeast CYC1 terminator, this part is in the Silver standard format. false false _331_ 0 2809 9 It's complicated false No special design considerations. false Daniel Jedrysiak annotation2012247 1 stop codon range2012247 1 1 3 annotation2012248 1 CYC1 Terminator range2012248 1 4 221 BBa_J63006 1 GAL1 yeast GAL1 promoter 2006-10-10T11:00:00Z 2015-08-31T01:56:26Z genomic GAL1 locus Released HQ 2013 GAL1 promoter from S. cerevisiae false true _97_ 0 545 97 In stock false unchanged from genomic sequence true Caroline Ajo-Franklin BBa_K234091 1 BBa_K234091 GFP, Silver standard 2009-07-21T11:00:00Z 2015-05-08T01:11:35Z This part was PCR amplified out of the pRS4D1T123 plasmid which was generously given to us by Dr. James J. Collins Ref: Kevin F. Murphy, G??bor Bal??zsi, and James J. Collins. Combinatorial promoter design for engineering noisy gene expression. PNAS 104(31), 12726???12731 (2007). This is a GFP reporter in the Silver standard (Assembly standard 23)format. It does not contain a start or stop codon. false false _331_ 0 2809 9 Not in stock false No special design considerations. false Daniel Jedrysiak annotation2012246 1 GFP range2012246 1 1 709 BBa_J63006_sequence 1 ccccattatcttagcctaaaaaaaccttctctttggaactttcagtaatacgcttaactgctcattgctatattgaagtacggattagaagccgccgagcgggtgacagccctccgaaggaagactctcctccgtgcgtcctcgtcttcaccggtcgcgttcctgaaacgcagatgtgcctcgcgccgcactgctccgaacaataaagattctacaatactagcttttatggttatgaagaggaaaaattggcagtaacctggccccacaaaccttcaaatgaacgaatcaaattaacaaccataggatgataatgcgattagttttttagccttatttctggggtaattaatcagcgaagcgatgatttttgatctattaacagatatataaatgcaaaaactgcataaccactttaactaatactttcaacattttcggtttgtattacttcttattcaaatgtaataaaagtatcaacaaaaaattgttaatatacctctatactttaacgtcaaggaggaaactagacccgccgccaccatggag BBa_K234091_sequence 1 taaaggtgaagaattattcactggtgttgtcccaattttggttgaattagatggtgatgttaatggtcacaaattttctgtctccggtgaaggtgaaggtgatgctacttacggtaaattgaccttaaaatttatttgtactactggtaaattgccagttccatggccaaccttagtcactactttcggttatggtgttcaatgttttgctagatacccagatcatatgaaacaacatgactttttcaagtctgccatgccagaaggttatgttcaagaaagaactatttttttcaaagatgacggtaactacaagaccagagctgaagtcaagtttgaaggtgataccttagttaatagaatcgaattaaaaggtattgattttaaagaagatggtaacattttaggtcacaaattggaatacaactataactctcacaatgtttacatcatggctgacaaacaaaagaatggtatcaaagttaacttcaaaattagacacaacattgaagatggttctgttcaattagctgaccattatcaacaaaatactccaattggtgatggtccagtcttgttaccagacaaccattacttatccactcaatctgccttatccaaagatccaaacgaaaagagagaccacatggtcttgttagaatttgttactgctgctggtattacccatggtatggatgaattgtacaaa BBa_K234092_sequence 1 taagatccgctctaaccgaaaaggaaggagttagacaacctgaagtctaggtccctatttatttttttatagttatgttagtattaagaacgttatttatatttcaaatttttcttttttttctgtacagacgcgtgtacgcatgtaacattatactgaaaaccttgcttgagaaggttttgggacgctcgaagatccagctgcattaatgaatcggccaa BBa_K234093_sequence 1 ccccattatcttagcctaaaaaaaccttctctttggaactttcagtaatacgcttaactgctcattgctatattgaagtacggattagaagccgccgagcgggtgacagccctccgaaggaagactctcctccgtgcgtcctcgtcttcaccggtcgcgttcctgaaacgcagatgtgcctcgcgccgcactgctccgaacaataaagattctacaatactagcttttatggttatgaagaggaaaaattggcagtaacctggccccacaaaccttcaaatgaacgaatcaaattaacaaccataggatgataatgcgattagttttttagccttatttctggggtaattaatcagcgaagcgatgatttttgatctattaacagatatataaatgcaaaaactgcataaccactttaactaatactttcaacattttcggtttgtattacttcttattcaaatgtaataaaagtatcaacaaaaaattgttaatatacctctatactttaacgtcaaggaggaaactagacccgccgccaccatggagtactagagtaaaggtgaagaattattcactggtgttgtcccaattttggttgaattagatggtgatgttaatggtcacaaattttctgtctccggtgaaggtgaaggtgatgctacttacggtaaattgaccttaaaatttatttgtactactggtaaattgccagttccatggccaaccttagtcactactttcggttatggtgttcaatgttttgctagatacccagatcatatgaaacaacatgactttttcaagtctgccatgccagaaggttatgttcaagaaagaactatttttttcaaagatgacggtaactacaagaccagagctgaagtcaagtttgaaggtgataccttagttaatagaatcgaattaaaaggtattgattttaaagaagatggtaacattttaggtcacaaattggaatacaactataactctcacaatgtttacatcatggctgacaaacaaaagaatggtatcaaagttaacttcaaaattagacacaacattgaagatggttctgttcaattagctgaccattatcaacaaaatactccaattggtgatggtccagtcttgttaccagacaaccattacttatccactcaatctgccttatccaaagatccaaacgaaaagagagaccacatggtcttgttagaatttgttactgctgctggtattacccatggtatggatgaattgtacaaatactagagtaagatccgctctaaccgaaaaggaaggagttagacaacctgaagtctaggtccctatttatttttttatagttatgttagtattaagaacgttatttatatttcaaatttttcttttttttctgtacagacgcgtgtacgcatgtaacattatactgaaaaccttgcttgagaaggttttgggacgctcgaagatccagctgcattaatgaatcggccaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z