LOCUS       BBa_K235000              544 bp    DNA     linear       25-Nov-2024
DEFINITION  [R0010][J23066] (pLac+ribokey+stop)
ACCESSION   BBa_K235000
VERSION     BBa_K235000.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    209..302
                     /label=BBa_J23008
     misc_feature    311..407
                     /label=BBa_J23009
     stem_loop       427..470
                     /label=stem_loop
     misc_feature    416..495
                     /label=BBa_B0010
     terminator      416..495
                     /label=BBa_B0010
     stem_loop       511..530
                     /label=T7 TE
     misc_feature    537..537
                     /label=stop
     polyA_site      531..544
                     /label=polya
     misc_feature    504..544
                     /label=BBa_B0012
     terminator      504..544
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagac ccaaaagcaa gaggtgattc tagttggtgg
      241 ttaatgaaaa ttaacttact tactagaaat atctctaaaa agccagatta ttaatccggc
      301 tttactagag cgggcgggcg ggcgggcggg atccataaaa aaaaaaccca aaagcaagag
      361 gtgattctag ttaaaaaaaa aaagcttccc gcccgcccgc ccgcccgtac tagagccagg
      421 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
      481 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt
      541 tata
//