LOCUS       BBa_K235013             1501 bp    DNA     linear       22-Nov-2024
DEFINITION  [K145303][K235000] (ribokey-mediated pLac-controlled GFP reporter)
ACCESSION   BBa_K235013
VERSION     BBa_K235013.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23109
     misc_feature    44..86
                     /label=BBa_J23032
     CDS             93..812
                     /label=GFP protein
     CDS             93..812
                     /label=BBa_E0040
     stem_loop       832..875
                     /label=stem_loop
     misc_feature    821..900
                     /label=BBa_B0010
     terminator      821..900
                     /label=BBa_B0010
     stem_loop       916..935
                     /label=T7 TE
     misc_feature    942..942
                     /label=stop
     polyA_site      936..949
                     /label=polya
     misc_feature    909..949
                     /label=BBa_B0012
     terminator      909..949
                     /label=BBa_B0012
     CDS             958..1045
                     /label=end of LacI coding region (inactive)
     misc_feature    958..1157
                     /label=BBa_R0010
     misc_feature    1046..1083
                     /label=CAP binding site
     promoter        1094..1099
                     /label=-35
     promoter        1118..1123
                     /label=-10
     misc_feature    1123..1157
                     /label=LacI binding site
     misc_feature    1130..1130
                     /label=start
     promoter        958..1157
                     /label=BBa_R0010
     misc_feature    1166..1259
                     /label=BBa_J23008
     misc_feature    1268..1364
                     /label=BBa_J23009
     stem_loop       1384..1427
                     /label=stem_loop
     misc_feature    1373..1452
                     /label=BBa_B0010
     terminator      1373..1452
                     /label=BBa_B0010
     stem_loop       1468..1487
                     /label=T7 TE
     misc_feature    1494..1494
                     /label=stop
     polyA_site      1488..1501
                     /label=polya
     misc_feature    1461..1501
                     /label=BBa_B0012
     terminator      1461..1501
                     /label=BBa_B0012
ORIGIN
        1 tttacagcta gctcagtcct agggactgtg ctagctacta gagaactaga atcacctctt
       61 gcttttgggt aagacagaag aggagatact agatgcgtaa aggagaagaa cttttcactg
      121 gagttgtccc aattcttgtt gaattagatg gtgatgttaa tgggcacaaa ttttctgtca
      181 gtggagaggg tgaaggtgat gcaacatacg gaaaacttac ccttaaattt atttgcacta
      241 ctggaaaact acctgttcca tggccaacac ttgtcactac tttcggttat ggtgttcaat
      301 gctttgcgag atacccagat catatgaaac agcatgactt tttcaagagt gccatgcccg
      361 aaggttatgt acaggaaaga actatatttt tcaaagatga cgggaactac aagacacgtg
      421 ctgaagtcaa gtttgaaggt gatacccttg ttaatagaat cgagttaaaa ggtattgatt
      481 ttaaagaaga tggaaacatt cttggacaca aattggaata caactataac tcacacaatg
      541 tatacatcat ggcagacaaa caaaagaatg gaatcaaagt taacttcaaa attagacaca
      601 acattgaaga tggaagcgtt caactagcag accattatca acaaaatact ccaattggcg
      661 atggccctgt ccttttacca gacaaccatt acctgtccac acaatctgcc ctttcgaaag
      721 atcccaacga aaagagagac cacatggtcc ttcttgagtt tgtaacagct gctgggatta
      781 cacatggcat ggatgaacta tacaaataat aatactagag ccaggcatca aataaaacga
      841 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
      901 tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat actagagcaa
      961 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     1021 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     1081 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     1141 gataacaatt tcacacatac tagagaccca aaagcaagag gtgattctag ttggtggtta
     1201 atgaaaatta acttacttac tagaaatatc tctaaaaagc cagattatta atccggcttt
     1261 actagagcgg gcgggcgggc gggcgggatc cataaaaaaa aaacccaaaa gcaagaggtg
     1321 attctagtta aaaaaaaaaa gcttcccgcc cgcccgcccg cccgtactag agccaggcat
     1381 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
     1441 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat
     1501 a
//