BBa_K235013
type: ComponentDefinition
persistent identity: BBa K235013
display id: BBa_K235013
version: 1
was generated by: iGEM to SBOL conversion
description: [K145303][K235000] (ribokey-mediated pLac-controlled GFP reporter)
created: 2009-09-02T11:00:00Z
modified: 2015-05-08T01:11:35Z
mutable provenance: K145303+K235000
owned by: james
owned by: myers
mutable description: This composite part will constitutively produce ribolocked GFP mRNA, and will produce the ribokey needed for transcription only when induced by lactose or an analogue like IPTG. This ought to reduce signal noise due to transient pLac activity.
discontinued: false
dominant: false
experience: experience/None
group u list: _336_
m user id: 0
owner id: 5167
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Constructed using three-antibiotic assembly.
star: false
creator: Kyliah Clarkson
type: DnaRegion
role: SO:0000804
role: partType/Reporter
component: BBa_J23009
component: BBa_J23008
component: BBa_B0012
component: BBa_B0010
component: BBa_E0040
component: BBa_R0010
component: BBa_J23032
component: BBa_J23109
sequence annotation: BBa_J23109
sequence annotation: BBa_J23032
sequence annotation: BBa_E0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_R0010
sequence annotation: BBa_J23008
sequence annotation: BBa_J23009
sequence: https://synbiohub.org/public/igem/BBa_K235013_sequence/1

BBa_J23009
type: Component
persistent identity: component2018412
display id: component2018412
version: 1
top level: BBa_K235013
definition: BBa_J23009
access: public

BBa_J23008
type: Component
persistent identity: component2018411
display id: component2018411
version: 1
top level: BBa_K235013
definition: BBa_J23008
access: public

BBa_B0012
type: Component
persistent identity: component2018415
display id: component2018415
version: 1
top level: BBa_K235013
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component2018413
display id: component2018413
version: 1
top level: BBa_K235013
definition: BBa_B0010
access: public

BBa_B0010
type: Component
persistent identity: component2018398
display id: component2018398
version: 1
top level: BBa_K235013
definition: BBa_B0010
access: public

BBa_E0040
type: Component
persistent identity: component2018397
display id: component2018397
version: 1
top level: BBa_K235013
definition: GFP
access: public

BBa_R0010
type: Component
persistent identity: component2018404
display id: component2018404
version: 1
top level: BBa_K235013
definition: LacI
access: public

BBa_B0012
type: Component
persistent identity: component2018400
display id: component2018400
version: 1
top level: BBa_K235013
definition: BBa_B0012
access: public

BBa_J23032
type: Component
persistent identity: component2018395
display id: component2018395
version: 1
top level: BBa_K235013
definition: BBa_J23032
access: public

BBa_J23109
type: Component
persistent identity: component2018394
display id: component2018394
version: 1
top level: BBa_K235013
definition: BBa_J23109
access: public

BBa_J23109
type: SequenceAnnotation
persistent identity: annotation2018394
display id: annotation2018394
version: 1
top level: BBa_K235013
location: https://synbiohub.org/public/igem/BBa_K235013/annotation2018394/range2018394/1
component: BBa_J23109

https://synbiohub.org/public/igem/BBa_K235013/annotation2018394/range2018394/1
type: Range
persistent identity: range2018394
display id: range2018394
version: 1
top level: BBa_K235013
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_J23032
type: SequenceAnnotation
persistent identity: annotation2018395
display id: annotation2018395
version: 1
top level: BBa_K235013
location: https://synbiohub.org/public/igem/BBa_K235013/annotation2018395/range2018395/1
component: BBa_J23032

https://synbiohub.org/public/igem/BBa_K235013/annotation2018395/range2018395/1
type: Range
persistent identity: range2018395
display id: range2018395
version: 1
top level: BBa_K235013
direction: direction/reverse
start: 44
end: 86
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2018397
display id: annotation2018397
version: 1
top level: BBa_K235013
location: https://synbiohub.org/public/igem/BBa_K235013/annotation2018397/range2018397/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K235013/annotation2018397/range2018397/1
type: Range
persistent identity: range2018397
display id: range2018397
version: 1
top level: BBa_K235013
direction: direction/reverse
start: 93
end: 812
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2018398
display id: annotation2018398
version: 1
top level: BBa_K235013
location: https://synbiohub.org/public/igem/BBa_K235013/annotation2018398/range2018398/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K235013/annotation2018398/range2018398/1
type: Range
persistent identity: range2018398
display id: range2018398
version: 1
top level: BBa_K235013
direction: direction/forward
start: 821
end: 900
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2018400
display id: annotation2018400
version: 1
top level: BBa_K235013
location: https://synbiohub.org/public/igem/BBa_K235013/annotation2018400/range2018400/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K235013/annotation2018400/range2018400/1
type: Range
persistent identity: range2018400
display id: range2018400
version: 1
top level: BBa_K235013
direction: direction/forward
start: 909
end: 949
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2018404
display id: annotation2018404
version: 1
top level: BBa_K235013
location: https://synbiohub.org/public/igem/BBa_K235013/annotation2018404/range2018404/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K235013/annotation2018404/range2018404/1
type: Range
persistent identity: range2018404
display id: range2018404
version: 1
top level: BBa_K235013
direction: direction/forward
start: 958
end: 1157
orientation: inline

BBa_J23008
type: SequenceAnnotation
persistent identity: annotation2018411
display id: annotation2018411
version: 1
top level: BBa_K235013
location: https://synbiohub.org/public/igem/BBa_K235013/annotation2018411/range2018411/1
component: BBa_J23008

https://synbiohub.org/public/igem/BBa_K235013/annotation2018411/range2018411/1
type: Range
persistent identity: range2018411
display id: range2018411
version: 1
top level: BBa_K235013
direction: direction/reverse
start: 1166
end: 1259
orientation: inline

BBa_J23009
type: SequenceAnnotation
persistent identity: annotation2018412
display id: annotation2018412
version: 1
top level: BBa_K235013
location: https://synbiohub.org/public/igem/BBa_K235013/annotation2018412/range2018412/1
component: BBa_J23009

https://synbiohub.org/public/igem/BBa_K235013/annotation2018412/range2018412/1
type: Range
persistent identity: range2018412
display id: range2018412
version: 1
top level: BBa_K235013
direction: direction/reverse
start: 1268
end: 1364
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2018413
display id: annotation2018413
version: 1
top level: BBa_K235013
location: https://synbiohub.org/public/igem/BBa_K235013/annotation2018413/range2018413/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K235013/annotation2018413/range2018413/1
type: Range
persistent identity: range2018413
display id: range2018413
version: 1
top level: BBa_K235013
direction: direction/forward
start: 1373
end: 1452
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2018415
display id: annotation2018415
version: 1
top level: BBa_K235013
location: https://synbiohub.org/public/igem/BBa_K235013/annotation2018415/range2018415/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K235013/annotation2018415/range2018415/1
type: Range
persistent identity: range2018415
display id: range2018415
version: 1
top level: BBa_K235013
direction: direction/forward
start: 1461
end: 1501
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_R0010
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 88
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 200
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
top level: LacI
direction: direction/forward
start: 89
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
top level: LacI
direction: direction/forward
start: 137
end: 142
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
top level: LacI
direction: direction/forward
start: 161
end: 166
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
top level: LacI
direction: direction/forward
start: 166
end: 200
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
top level: LacI
direction: direction/forward
start: 173
end: 173
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

BBa_J23008
type: ComponentDefinition
persistent identity: BBa J23008
display id: BBa_J23008
version: 1
was generated by: iGEM to SBOL conversion
description: [key3c] riboregulator for lock3 variants
created: 2006-07-09T11:00:00Z
modified: 2015-08-31T04:08:38Z
mutable provenance: J23007
owned by: james
owned by: myers
mutable description: This part has the same homology region to J01122 as J23007 except that unlike J23007, J23008 does not contain the hairpin that was derived from J01129. J23008 anneals linearly to the stem of the hairpin in J01122 with perfect base pairing and destroys the hairpin in the process and reveals the RBS on J01122. When J01122 is transcribed the RNA secondary structure is such that the RBS hairpins with sequence upstream preventing the ribosome from binding and translation from occuring thereby locking up RFP further down stream. When J23008 is introduced into the cell in trans, we suspect that it will unlock the RBS for RFP as described above allowing translation to occur.
discontinued: false
dominant: false
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 931
owning group id: 52
sample status: In stock
status: status/Available
bookmark: false
mutable notes: No design considerations
star: true
creator: Kaitlin Davis
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence: https://synbiohub.org/public/igem/BBa_J23008_sequence/1

BBa_J23009
type: ComponentDefinition
persistent identity: BBa J23009
display id: BBa_J23009
version: 1
was generated by: iGEM to SBOL conversion
description: [key3d] riboregulator for lock3 variants
created: 2006-08-02T11:00:00Z
modified: 2015-08-31T04:08:38Z
mutable provenance: Overlap extension
owned by: james
owned by: myers
mutable description: Variant of key3 (see part J01129 (key3), J23007(key3b), J23008(key3c)). Part is in pJ23006, so there is a Ptet upstream of the XbaI site to drive expression. Nevertheless, J23009 is still a basic part allowing both prefix and suffix insertions.
discontinued: false
dominant: false
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence: https://synbiohub.org/public/igem/BBa_J23009_sequence/1

BBa_J23032
type: ComponentDefinition
persistent identity: BBa J23032
display id: BBa_J23032
version: 1
was generated by: iGEM to SBOL conversion
description: [lock3d]
created: 2006-08-02T11:00:00Z
modified: 2015-08-31T04:08:39Z
mutable provenance: Extension of overlapping oligonucleotides
owned by: james
owned by: myers
mutable description: lock3d
discontinued: false
dominant: false
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: partType/RNA
role: SO:0000834
sequence: https://synbiohub.org/public/igem/BBa_J23032_sequence/1

BBa_J23109
type: ComponentDefinition
persistent identity: BBa J23109
display id: BBa_J23109
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Later
owned by: james
owned by: myers
mutable description: Later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23109_sequence/1

https://synbiohub.org/public/igem/BBa_K235013_sequence/1
type: Sequence
persistent identity: BBa K235013 sequence
display id: BBa_K235013_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacagctagctcagtcctagggactgtgctagctactagagaactagaatcacctcttgcttttgggtaagacagaagaggagatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagacccaaaagcaagaggtgattctagttggtggttaatgaaaattaacttacttactagaaatatctctaaaaagccagattattaatccggctttactagagcgggcgggcgggcgggcgggatccataaaaaaaaaacccaaaagcaagaggtgattctagttaaaaaaaaaaagcttcccgcccgcccgcccgcccgtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0010_sequence/1
type: Sequence
persistent identity: BBa R0010 sequence
display id: BBa_R0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23008_sequence/1
type: Sequence
persistent identity: BBa J23008 sequence
display id: BBa_J23008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acccaaaagcaagaggtgattctagttggtggttaatgaaaattaacttacttactagaaatatctctaaaaagccagattattaatccggctt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23009_sequence/1
type: Sequence
persistent identity: BBa J23009 sequence
display id: BBa_J23009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cgggcgggcgggcgggcgggatccataaaaaaaaaacccaaaagcaagaggtgattctagttaaaaaaaaaaagcttcccgcccgcccgcccgcccg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23032_sequence/1
type: Sequence
persistent identity: BBa J23032 sequence
display id: BBa_J23032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aactagaatcacctcttgcttttgggtaagacagaagaggaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23109_sequence/1
type: Sequence
persistent identity: BBa J23109 sequence
display id: BBa_J23109_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacagctagctcagtcctagggactgtgctagc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z