LOCUS       BBa_K235018              926 bp    DNA     linear       25-Nov-2024
DEFINITION  [K235015][K235005] pLambda-controlled mCherry generator
ACCESSION   BBa_K235018
VERSION     BBa_K235018.1  
FEATURES             Location/Qualifiers
     promoter        38..43
                     /label=-10
     promoter        15..20
                     /label=-35
     misc_feature    25..41
                     /label=OR1
     misc_feature    1..17
                     /label=OR2
     misc_feature    1..49
                     /label=BBa_R0051
     promoter        1..49
                     /label=BBa_R0051
     misc_feature    62..65
                     /label=conserved
     RBS             58..69
                     /label=BBa_B0034
     CDS             76..786
                     /label=mCherry
     misc_feature    427..427
                     /label=C->T (removing PstI site)
     misc_feature    76..789
                     /label=BBa_J06504
     stem_loop       809..852
                     /label=stem_loop
     misc_feature    798..877
                     /label=BBa_B0010
     terminator      798..877
                     /label=BBa_B0010
     stem_loop       893..912
                     /label=T7 TE
     misc_feature    919..919
                     /label=stop
     polyA_site      913..926
                     /label=polya
     misc_feature    886..926
                     /label=BBa_B0012
     terminator      886..926
                     /label=BBa_B0012
ORIGIN
        1 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgct actagagaaa
       61 gaggagaaat actagatggt gagcaagggc gaggaggata acatggccat catcaaggag
      121 ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag
      181 ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag
      241 ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag
      301 gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc
      361 ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac
      421 tcctccttgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc
      481 tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg
      541 taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc
      601 ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc
      661 ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc
      721 gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac
      781 aagtaataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
      841 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc
      901 ttcgggtggg cctttctgcg tttata
//