LOCUS       BBa_K235019             2179 bp    DNA     linear       02-Jul-2024
DEFINITION  [K235016][K235003] (ribokey-mediated pAra-controlled lambda 
            repressor generator)
ACCESSION   BBa_K235019
VERSION     BBa_K235019.1  
FEATURES             Location/Qualifiers
     promoter        1080..1210
                     /label=Pbad
     promoter        1043..1114
                     /label=PC
     misc_feature    1..1079
                     /label=AraC
     promoter        1..1210
                     /label=BBa_I0500
     misc_feature    1219..1261
                     /label=BBa_J23032
     misc_feature    1..1261
                     /label=BBa_K235016
     CDS             1271..1978
                     /label=cI lambda
     misc_feature    1979..2011
                     /label=LVA
     misc_feature    2018..2042
                     /label=Help:Barcodes
     CDS             1268..2042
                     /label=BBa_C0051
     stem_loop       2062..2105
                     /label=stem_loop
     misc_feature    2051..2130
                     /label=BBa_B0010
     terminator      2051..2130
                     /label=BBa_B0010
     stem_loop       2146..2165
                     /label=T7 TE
     misc_feature    2172..2172
                     /label=stop
     polyA_site      2166..2179
                     /label=polya
     misc_feature    2139..2179
                     /label=BBa_B0012
     terminator      2139..2179
                     /label=BBa_B0012
     terminator      2051..2179
                     /label=BBa_B0015
     misc_feature    1268..2179
                     /label=BBa_K235003
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc tactagagaa ctagaatcac ctcttgcttt tgggtaagac agaagaggag
     1261 atactagatg agcacaaaaa agaaaccatt aacacaagag cagcttgagg acgcacgtcg
     1321 ccttaaagca atttatgaaa aaaagaaaaa tgaacttggc ttatcccagg aatctgtcgc
     1381 agacaagatg gggatggggc agtcaggcgt tggtgcttta tttaatggca tcaatgcatt
     1441 aaatgcttat aacgccgcat tgcttgcaaa aattctcaaa gttagcgttg aagaatttag
     1501 cccttcaatc gccagagaaa tctacgagat gtatgaagcg gttagtatgc agccgtcact
     1561 tagaagtgag tatgagtacc ctgttttttc tcatgttcag gcagggatgt tctcacctga
     1621 gcttagaacc tttaccaaag gtgatgcgga gagatgggta agcacaacca aaaaagccag
     1681 tgattctgca ttctggcttg aggttgaagg taattccatg accgcaccaa caggctccaa
     1741 gccaagcttt cctgacggaa tgttaattct cgttgaccct gagcaggctg ttgagccagg
     1801 tgatttctgc atagccagac ttgggggtga tgagtttacc ttcaagaaac tgatcaggga
     1861 tagcggtcag gtgtttttac aaccactaaa cccacagtac ccaatgatcc catgcaatga
     1921 gagttgttcc gttgtgggga aagttatcgc tagtcagtgg cctgaagaga cgtttggcgc
     1981 tgcaaacgac gaaaactacg ctttagtagc ttaataacgc tgatagtgct agtgtagatc
     2041 gctactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
     2101 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
     2161 gggcctttct gcgtttata
//