LOCUS       BBa_K235020             2148 bp    DNA     linear       25-Nov-2024
DEFINITION  [K235017][K235003] (pAra-controlled lambda repressor generator)
ACCESSION   BBa_K235020
VERSION     BBa_K235020.1  
FEATURES             Location/Qualifiers
     promoter        1080..1210
                     /label=Pbad
     promoter        1043..1114
                     /label=PC
     misc_feature    1..1079
                     /label=AraC
     promoter        1..1210
                     /label=BBa_I0500
     misc_feature    1223..1226
                     /label=conserved
     RBS             1219..1230
                     /label=BBa_B0034
     misc_feature    1..1230
                     /label=BBa_K235017
     CDS             1240..1947
                     /label=cI lambda
     misc_feature    1948..1980
                     /label=LVA
     misc_feature    1987..2011
                     /label=Help:Barcodes
     CDS             1237..2011
                     /label=BBa_C0051
     stem_loop       2031..2074
                     /label=stem_loop
     misc_feature    2020..2099
                     /label=BBa_B0010
     terminator      2020..2099
                     /label=BBa_B0010
     stem_loop       2115..2134
                     /label=T7 TE
     misc_feature    2141..2141
                     /label=stop
     polyA_site      2135..2148
                     /label=polya
     misc_feature    2108..2148
                     /label=BBa_B0012
     terminator      2108..2148
                     /label=BBa_B0012
     terminator      2020..2148
                     /label=BBa_B0015
     misc_feature    1237..2148
                     /label=BBa_K235003
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc tactagagaa agaggagaaa tactagatga gcacaaaaaa gaaaccatta
     1261 acacaagagc agcttgagga cgcacgtcgc cttaaagcaa tttatgaaaa aaagaaaaat
     1321 gaacttggct tatcccagga atctgtcgca gacaagatgg ggatggggca gtcaggcgtt
     1381 ggtgctttat ttaatggcat caatgcatta aatgcttata acgccgcatt gcttgcaaaa
     1441 attctcaaag ttagcgttga agaatttagc ccttcaatcg ccagagaaat ctacgagatg
     1501 tatgaagcgg ttagtatgca gccgtcactt agaagtgagt atgagtaccc tgttttttct
     1561 catgttcagg cagggatgtt ctcacctgag cttagaacct ttaccaaagg tgatgcggag
     1621 agatgggtaa gcacaaccaa aaaagccagt gattctgcat tctggcttga ggttgaaggt
     1681 aattccatga ccgcaccaac aggctccaag ccaagctttc ctgacggaat gttaattctc
     1741 gttgaccctg agcaggctgt tgagccaggt gatttctgca tagccagact tgggggtgat
     1801 gagtttacct tcaagaaact gatcagggat agcggtcagg tgtttttaca accactaaac
     1861 ccacagtacc caatgatccc atgcaatgag agttgttccg ttgtggggaa agttatcgct
     1921 agtcagtggc ctgaagagac gtttggcgct gcaaacgacg aaaactacgc tttagtagct
     1981 taataacgct gatagtgcta gtgtagatcg ctactagagc caggcatcaa ataaaacgaa
     2041 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct
     2101 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata
//