LOCUS BBa_K235023 3082 bp DNA linear 25-Jun-2024 DEFINITION [K235018][K235020] (mCherry generator, pAra-controlled signal inversion) ACCESSION BBa_K235023 VERSION BBa_K235023.1 FEATURES Location/Qualifiers promoter 38..43 /label=-10 promoter 15..20 /label=-35 misc_feature 25..41 /label=OR1 misc_feature 1..17 /label=OR2 misc_feature 1..49 /label=BBa_R0051 promoter 1..49 /label=BBa_R0051 misc_feature 62..65 /label=conserved RBS 58..69 /label=BBa_B0034 CDS 76..786 /label=mCherry misc_feature 427..427 /label=C->T (removing PstI site) misc_feature 76..789 /label=BBa_J06504 stem_loop 809..852 /label=stem_loop misc_feature 798..877 /label=BBa_B0010 terminator 798..877 /label=BBa_B0010 stem_loop 893..912 /label=T7 TE misc_feature 919..919 /label=stop polyA_site 913..926 /label=polya misc_feature 886..926 /label=BBa_B0012 terminator 886..926 /label=BBa_B0012 misc_feature 1..926 /label=BBa_K235018 promoter 2014..2144 /label=Pbad promoter 1977..2048 /label=PC misc_feature 935..2013 /label=AraC promoter 935..2144 /label=BBa_I0500 misc_feature 2157..2160 /label=conserved RBS 2153..2164 /label=BBa_B0034 misc_feature 935..2164 /label=BBa_K235017 CDS 2174..2881 /label=cI lambda misc_feature 2882..2914 /label=LVA misc_feature 2921..2945 /label=Help:Barcodes CDS 2171..2945 /label=BBa_C0051 stem_loop 2965..3008 /label=stem_loop misc_feature 2954..3033 /label=BBa_B0010 terminator 2954..3033 /label=BBa_B0010 stem_loop 3049..3068 /label=T7 TE misc_feature 3075..3075 /label=stop polyA_site 3069..3082 /label=polya misc_feature 3042..3082 /label=BBa_B0012 terminator 3042..3082 /label=BBa_B0012 terminator 2954..3082 /label=BBa_B0015 misc_feature 2171..3082 /label=BBa_K235003 misc_feature 935..3082 /label=BBa_K235020 ORIGIN 1 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgct actagagaaa 61 gaggagaaat actagatggt gagcaagggc gaggaggata acatggccat catcaaggag 121 ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag 181 ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag 241 ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag 301 gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc 361 ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac 421 tcctccttgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc 481 tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg 541 taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc 601 ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc 661 ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc 721 gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac 781 aagtaataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc 841 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc 901 ttcgggtggg cctttctgcg tttatatact agagttatga caacttgacg gctacatcat 961 tcactttttc ttcacaaccg gcacggaact cgctcgggct ggccccggtg cattttttaa 1021 atacccgcga gaaatagagt tgatcgtcaa aaccaacatt gcgaccgacg gtggcgatag 1081 gcatccgggt ggtgctcaaa agcagcttcg cctggctgat acgttggtcc tcgcgccagc 1141 ttaagacgct aatccctaac tgctggcgga aaagatgtga cagacgcgac ggcgacaagc 1201 aaacatgctg tgcgacgctg gcgatatcaa aattgctgtc tgccaggtga tcgctgatgt 1261 actgacaagc ctcgcgtacc cgattatcca tcggtggatg gagcgactcg ttaatcgctt 1321 ccatgcgccg cagtaacaat tgctcaagca gatttatcgc cagcagctcc gaatagcgcc 1381 cttccccttg cccggcgtta atgatttgcc caaacaggtc gctgaaatgc ggctggtgcg 1441 cttcatccgg gcgaaagaac cccgtattgg caaatattga cggccagtta agccattcat 1501 gccagtaggc gcgcggacga aagtaaaccc actggtgata ccattcgcga gcctccggat 1561 gacgaccgta gtgatgaatc tctcctggcg ggaacagcaa aatatcaccc ggtcggcaaa 1621 caaattctcg tccctgattt ttcaccaccc cctgaccgcg aatggtgaga ttgagaatat 1681 aacctttcat tcccagcggt cggtcgataa aaaaatcgag ataaccgttg gcctcaatcg 1741 gcgttaaacc cgccaccaga tgggcattaa acgagtatcc cggcagcagg ggatcatttt 1801 gcgcttcagc catacttttc atactcccgc cattcagaga agaaaccaat tgtccatatt 1861 gcatcagaca ttgccgtcac tgcgtctttt actggctctt ctcgctaacc aaaccggtaa 1921 ccccgcttat taaaagcatt ctgtaacaaa gcgggaccaa agccatgaca aaaacgcgta 1981 acaaaagtgt ctataatcac ggcagaaaag tccacattga ttatttgcac ggcgtcacac 2041 tttgctatgc catagcattt ttatccataa gattagcgga tcctacctga cgctttttat 2101 cgcaactctc tactgtttct ccatacccgt ttttttgggc tagctactag agaaagagga 2161 gaaatactag atgagcacaa aaaagaaacc attaacacaa gagcagcttg aggacgcacg 2221 tcgccttaaa gcaatttatg aaaaaaagaa aaatgaactt ggcttatccc aggaatctgt 2281 cgcagacaag atggggatgg ggcagtcagg cgttggtgct ttatttaatg gcatcaatgc 2341 attaaatgct tataacgccg cattgcttgc aaaaattctc aaagttagcg ttgaagaatt 2401 tagcccttca atcgccagag aaatctacga gatgtatgaa gcggttagta tgcagccgtc 2461 acttagaagt gagtatgagt accctgtttt ttctcatgtt caggcaggga tgttctcacc 2521 tgagcttaga acctttacca aaggtgatgc ggagagatgg gtaagcacaa ccaaaaaagc 2581 cagtgattct gcattctggc ttgaggttga aggtaattcc atgaccgcac caacaggctc 2641 caagccaagc tttcctgacg gaatgttaat tctcgttgac cctgagcagg ctgttgagcc 2701 aggtgatttc tgcatagcca gacttggggg tgatgagttt accttcaaga aactgatcag 2761 ggatagcggt caggtgtttt tacaaccact aaacccacag tacccaatga tcccatgcaa 2821 tgagagttgt tccgttgtgg ggaaagttat cgctagtcag tggcctgaag agacgtttgg 2881 cgctgcaaac gacgaaaact acgctttagt agcttaataa cgctgatagt gctagtgtag 2941 atcgctacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt 3001 cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg 3061 ggtgggcctt tctgcgttta ta //