##gff-version 3
##sequence-region BBa_K235023 1 3082
BBa_K235023	.	engineered_region	1	926	.	+	0	ID=BBa_K235018;Name=BBa_K235018
BBa_K235023	.	terminator	798	877	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K235018
BBa_K235023	.	stem_loop	809	852	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K235023	.	engineered_region	798	877	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K235023	.	engineered_region	76	789	.	+	0	ID=BBa_J06504;Name=BBa_J06504;Parent=BBa_K235018
BBa_K235023	.	CDS	76	786	.	+	0	ID=annotation1585829;Name=mCherry;Parent=BBa_J06504
BBa_K235023	.	sequence_alteration	427	427	.	+	0	ID=annotation1585833;Name=C->T (removing PstI site);Parent=BBa_J06504
BBa_K235023	.	promoter	1	49	.	+	0	ID=BBa_R0051;Name=BBa_R0051;Parent=BBa_K235018
BBa_K235023	.	engineered_region	1	49	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_K235023	.	non_covalent_binding_site	25	41	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_K235023	.	promoter	38	43	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_K235023	.	non_covalent_binding_site	1	17	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_K235023	.	promoter	15	20	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_K235023	.	terminator	886	926	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K235018
BBa_K235023	.	stem_loop	893	912	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K235023	.	polyA_site	913	926	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K235023	.	stop_codon	919	919	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K235023	.	engineered_region	886	926	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K235023	.	ribosome_entry_site	58	69	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K235018
BBa_K235023	.	conserved_region	62	65	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K235023	.	engineered_region	935	3082	.	+	0	ID=BBa_K235020;Name=BBa_K235020
BBa_K235023	.	engineered_region	2171	3082	.	+	0	ID=BBa_K235003;Name=BBa_K235003;Parent=BBa_K235020
BBa_K235023	.	terminator	2954	3082	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K235003
BBa_K235023	.	terminator	3042	3082	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K235023	.	stem_loop	3049	3068	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K235023	.	polyA_site	3069	3082	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K235023	.	stop_codon	3075	3075	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K235023	.	engineered_region	3042	3082	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K235023	.	terminator	2954	3033	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K235023	.	stem_loop	2965	3008	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K235023	.	engineered_region	2954	3033	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K235023	.	CDS	2171	2945	.	+	0	ID=BBa_C0051;Name=BBa_C0051;Parent=BBa_K235003
BBa_K235023	.	engineered_tag	2921	2945	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_K235023	.	CDS	2174	2881	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_K235023	.	tag	2882	2914	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_K235023	.	engineered_region	935	2164	.	+	0	ID=BBa_K235017;Name=BBa_K235017;Parent=BBa_K235020
BBa_K235023	.	promoter	935	2144	.	+	0	ID=BBa_I0500;Name=BBa_I0500;Parent=BBa_K235017
BBa_K235023	.	promoter	1977	2048	.	+	0	ID=annotation1498842;Name=PC;Parent=BBa_I0500
BBa_K235023	.	promoter	2014	2144	.	+	0	ID=annotation1498841;Name=Pbad;Parent=BBa_I0500
BBa_K235023	.	sequence_feature	935	2013	.	+	0	ID=annotation1498843;Name=AraC;Parent=BBa_I0500
BBa_K235023	.	ribosome_entry_site	2153	2164	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K235017
BBa_K235023	.	conserved_region	2157	2160	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_K235023
taacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagaaagaggagaaatactagatgg
tgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaa
cggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgacc
aagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcacc
ccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacgg
cggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaac
ttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacg
gcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccaccta
caaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggac
tacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataat
actagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtga
acgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttatgacaacttga
cggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccg
cgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagc
ttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagac
gcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactg
acaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgc
tcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgc
tgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatg
ccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatc
tctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgc
gaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaat
cggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatactt
ttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggc
tcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaac
gcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccata
gcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttt
tttgggctagctactagagaaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgag
gacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaaga
tggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgc
aaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagt
atgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagctta
gaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggt
tgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgag
caggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggata
gcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaa
agttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgc
tgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcg
ttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
a