LOCUS BBa_K235025 3665 bp DNA linear 22-Dec-2024 DEFINITION [K235022][K235000] (NAND gate, pAra and pLac input signal control, mCherry output signal) ACCESSION BBa_K235025 VERSION BBa_K235025.1 FEATURES Location/Qualifiers promoter 38..43 /label=-10 promoter 15..20 /label=-35 misc_feature 25..41 /label=OR1 misc_feature 1..17 /label=OR2 misc_feature 1..49 /label=BBa_R0051 promoter 1..49 /label=BBa_R0051 misc_feature 62..65 /label=conserved RBS 58..69 /label=BBa_B0034 CDS 76..786 /label=mCherry misc_feature 427..427 /label=C->T (removing PstI site) misc_feature 76..789 /label=BBa_J06504 stem_loop 809..852 /label=stem_loop misc_feature 798..877 /label=BBa_B0010 terminator 798..877 /label=BBa_B0010 stem_loop 893..912 /label=T7 TE misc_feature 919..919 /label=stop polyA_site 913..926 /label=polya misc_feature 886..926 /label=BBa_B0012 terminator 886..926 /label=BBa_B0012 misc_feature 1..926 /label=BBa_K235018 promoter 2014..2144 /label=Pbad promoter 1977..2048 /label=PC misc_feature 935..2013 /label=AraC promoter 935..2144 /label=BBa_I0500 misc_feature 2153..2195 /label=BBa_J23032 misc_feature 935..2195 /label=BBa_K235016 CDS 2205..2912 /label=cI lambda misc_feature 2913..2945 /label=LVA misc_feature 2952..2976 /label=Help:Barcodes CDS 2202..2976 /label=BBa_C0051 stem_loop 2996..3039 /label=stem_loop misc_feature 2985..3064 /label=BBa_B0010 terminator 2985..3064 /label=BBa_B0010 stem_loop 3080..3099 /label=T7 TE misc_feature 3106..3106 /label=stop polyA_site 3100..3113 /label=polya misc_feature 3073..3113 /label=BBa_B0012 terminator 3073..3113 /label=BBa_B0012 terminator 2985..3113 /label=BBa_B0015 misc_feature 2202..3113 /label=BBa_K235003 misc_feature 935..3113 /label=BBa_K235019 misc_feature 1..3113 /label=BBa_K235022 CDS 3122..3209 /label=end of LacI coding region (inactive) misc_feature 3122..3321 /label=BBa_R0010 misc_feature 3210..3247 /label=CAP binding site promoter 3258..3263 /label=-35 promoter 3282..3287 /label=-10 misc_feature 3287..3321 /label=LacI binding site misc_feature 3294..3294 /label=start promoter 3122..3321 /label=BBa_R0010 misc_feature 3330..3423 /label=BBa_J23008 misc_feature 3432..3528 /label=BBa_J23009 stem_loop 3548..3591 /label=stem_loop misc_feature 3537..3616 /label=BBa_B0010 terminator 3537..3616 /label=BBa_B0010 stem_loop 3632..3651 /label=T7 TE misc_feature 3658..3658 /label=stop polyA_site 3652..3665 /label=polya misc_feature 3625..3665 /label=BBa_B0012 terminator 3625..3665 /label=BBa_B0012 misc_feature 3122..3665 /label=BBa_K235000 ORIGIN 1 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgct actagagaaa 61 gaggagaaat actagatggt gagcaagggc gaggaggata acatggccat catcaaggag 121 ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag 181 ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag 241 ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag 301 gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc 361 ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac 421 tcctccttgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc 481 tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg 541 taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc 601 ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc 661 ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc 721 gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac 781 aagtaataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc 841 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc 901 ttcgggtggg cctttctgcg tttatatact agagttatga caacttgacg gctacatcat 961 tcactttttc ttcacaaccg gcacggaact cgctcgggct ggccccggtg cattttttaa 1021 atacccgcga gaaatagagt tgatcgtcaa aaccaacatt gcgaccgacg gtggcgatag 1081 gcatccgggt ggtgctcaaa agcagcttcg cctggctgat acgttggtcc tcgcgccagc 1141 ttaagacgct aatccctaac tgctggcgga aaagatgtga cagacgcgac ggcgacaagc 1201 aaacatgctg tgcgacgctg gcgatatcaa aattgctgtc tgccaggtga tcgctgatgt 1261 actgacaagc ctcgcgtacc cgattatcca tcggtggatg gagcgactcg ttaatcgctt 1321 ccatgcgccg cagtaacaat tgctcaagca gatttatcgc cagcagctcc gaatagcgcc 1381 cttccccttg cccggcgtta atgatttgcc caaacaggtc gctgaaatgc ggctggtgcg 1441 cttcatccgg gcgaaagaac cccgtattgg caaatattga cggccagtta agccattcat 1501 gccagtaggc gcgcggacga aagtaaaccc actggtgata ccattcgcga gcctccggat 1561 gacgaccgta gtgatgaatc tctcctggcg ggaacagcaa aatatcaccc ggtcggcaaa 1621 caaattctcg tccctgattt ttcaccaccc cctgaccgcg aatggtgaga ttgagaatat 1681 aacctttcat tcccagcggt cggtcgataa aaaaatcgag ataaccgttg gcctcaatcg 1741 gcgttaaacc cgccaccaga tgggcattaa acgagtatcc cggcagcagg ggatcatttt 1801 gcgcttcagc catacttttc atactcccgc cattcagaga agaaaccaat tgtccatatt 1861 gcatcagaca ttgccgtcac tgcgtctttt actggctctt ctcgctaacc aaaccggtaa 1921 ccccgcttat taaaagcatt ctgtaacaaa gcgggaccaa agccatgaca aaaacgcgta 1981 acaaaagtgt ctataatcac ggcagaaaag tccacattga ttatttgcac ggcgtcacac 2041 tttgctatgc catagcattt ttatccataa gattagcgga tcctacctga cgctttttat 2101 cgcaactctc tactgtttct ccatacccgt ttttttgggc tagctactag agaactagaa 2161 tcacctcttg cttttgggta agacagaaga ggagatacta gatgagcaca aaaaagaaac 2221 cattaacaca agagcagctt gaggacgcac gtcgccttaa agcaatttat gaaaaaaaga 2281 aaaatgaact tggcttatcc caggaatctg tcgcagacaa gatggggatg gggcagtcag 2341 gcgttggtgc tttatttaat ggcatcaatg cattaaatgc ttataacgcc gcattgcttg 2401 caaaaattct caaagttagc gttgaagaat ttagcccttc aatcgccaga gaaatctacg 2461 agatgtatga agcggttagt atgcagccgt cacttagaag tgagtatgag taccctgttt 2521 tttctcatgt tcaggcaggg atgttctcac ctgagcttag aacctttacc aaaggtgatg 2581 cggagagatg ggtaagcaca accaaaaaag ccagtgattc tgcattctgg cttgaggttg 2641 aaggtaattc catgaccgca ccaacaggct ccaagccaag ctttcctgac ggaatgttaa 2701 ttctcgttga ccctgagcag gctgttgagc caggtgattt ctgcatagcc agacttgggg 2761 gtgatgagtt taccttcaag aaactgatca gggatagcgg tcaggtgttt ttacaaccac 2821 taaacccaca gtacccaatg atcccatgca atgagagttg ttccgttgtg gggaaagtta 2881 tcgctagtca gtggcctgaa gagacgtttg gcgctgcaaa cgacgaaaac tacgctttag 2941 tagcttaata acgctgatag tgctagtgta gatcgctact agagccaggc atcaaataaa 3001 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc 3061 tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga 3121 gcaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac 3181 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact 3241 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg 3301 agcggataac aatttcacac atactagaga cccaaaagca agaggtgatt ctagttggtg 3361 gttaatgaaa attaacttac ttactagaaa tatctctaaa aagccagatt attaatccgg 3421 ctttactaga gcgggcgggc gggcgggcgg gatccataaa aaaaaaaccc aaaagcaaga 3481 ggtgattcta gttaaaaaaa aaaagcttcc cgcccgcccg cccgcccgta ctagagccag 3541 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt 3601 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt 3661 ttata //