##gff-version 3
##sequence-region BBa_K235025 1 3665
BBa_K235025	.	engineered_region	3122	3665	.	+	0	ID=BBa_K235000;Name=BBa_K235000
BBa_K235025	.	terminator	3537	3616	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K235000
BBa_K235025	.	stem_loop	3548	3591	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K235025	.	engineered_region	3537	3616	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K235025	.	terminator	3625	3665	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K235000
BBa_K235025	.	polyA_site	3652	3665	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K235025	.	stop_codon	3658	3658	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K235025	.	engineered_region	3625	3665	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K235025	.	stem_loop	3632	3651	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K235025	.	promoter	3122	3321	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_K235000
BBa_K235025	.	engineered_region	3122	3321	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K235025	.	promoter	3258	3263	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K235025	.	non_covalent_binding_site	3210	3247	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K235025	.	promoter	3282	3287	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K235025	.	CDS	3122	3209	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K235025	.	non_covalent_binding_site	3287	3321	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K235025	.	start_codon	3294	3294	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K235025	.	mature_transcript_region	3330	3423	.	+	0	ID=BBa_J23008;Name=BBa_J23008;Parent=BBa_K235000
BBa_K235025	.	mature_transcript_region	3432	3528	.	+	0	ID=BBa_J23009;Name=BBa_J23009;Parent=BBa_K235000
BBa_K235025	.	engineered_region	1	3113	.	+	0	ID=BBa_K235022;Name=BBa_K235022
BBa_K235025	.	engineered_region	1	926	.	+	0	ID=BBa_K235018;Name=BBa_K235018;Parent=BBa_K235022
BBa_K235025	.	terminator	798	877	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K235018
BBa_K235025	.	stem_loop	809	852	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K235025	.	engineered_region	798	877	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K235025	.	promoter	1	49	.	+	0	ID=BBa_R0051;Name=BBa_R0051;Parent=BBa_K235018
BBa_K235025	.	engineered_region	1	49	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_K235025	.	non_covalent_binding_site	25	41	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_K235025	.	promoter	15	20	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_K235025	.	promoter	38	43	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_K235025	.	non_covalent_binding_site	1	17	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_K235025	.	ribosome_entry_site	58	69	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K235018
BBa_K235025	.	conserved_region	62	65	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K235025	.	terminator	886	926	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K235018
BBa_K235025	.	polyA_site	913	926	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K235025	.	stop_codon	919	919	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K235025	.	engineered_region	886	926	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K235025	.	stem_loop	893	912	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K235025	.	engineered_region	76	789	.	+	0	ID=BBa_J06504;Name=BBa_J06504;Parent=BBa_K235018
BBa_K235025	.	CDS	76	786	.	+	0	ID=annotation1585829;Name=mCherry;Parent=BBa_J06504
BBa_K235025	.	sequence_alteration	427	427	.	+	0	ID=annotation1585833;Name=C->T (removing PstI site);Parent=BBa_J06504
BBa_K235025	.	engineered_region	935	3113	.	+	0	ID=BBa_K235019;Name=BBa_K235019;Parent=BBa_K235022
BBa_K235025	.	engineered_region	935	2195	.	+	0	ID=BBa_K235016;Name=BBa_K235016;Parent=BBa_K235019
BBa_K235025	.	promoter	935	2144	.	+	0	ID=BBa_I0500;Name=BBa_I0500;Parent=BBa_K235016
BBa_K235025	.	promoter	2014	2144	.	+	0	ID=annotation1498841;Name=Pbad;Parent=BBa_I0500
BBa_K235025	.	sequence_feature	935	2013	.	+	0	ID=annotation1498843;Name=AraC;Parent=BBa_I0500
BBa_K235025	.	promoter	1977	2048	.	+	0	ID=annotation1498842;Name=PC;Parent=BBa_I0500
BBa_K235025	.	mature_transcript_region	2153	2195	.	+	0	ID=BBa_J23032;Name=BBa_J23032;Parent=BBa_K235016
BBa_K235025	.	engineered_region	2202	3113	.	+	0	ID=BBa_K235003;Name=BBa_K235003;Parent=BBa_K235019
BBa_K235025	.	terminator	2985	3113	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K235003
BBa_K235025	.	terminator	3073	3113	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K235025	.	polyA_site	3100	3113	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K235025	.	stop_codon	3106	3106	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K235025	.	engineered_region	3073	3113	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K235025	.	stem_loop	3080	3099	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K235025	.	terminator	2985	3064	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K235025	.	stem_loop	2996	3039	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K235025	.	engineered_region	2985	3064	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K235025	.	CDS	2202	2976	.	+	0	ID=BBa_C0051;Name=BBa_C0051;Parent=BBa_K235003
BBa_K235025	.	engineered_tag	2952	2976	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_K235025	.	tag	2913	2945	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_K235025	.	CDS	2205	2912	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
>BBa_K235025
taacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagaaagaggagaaatactagatgg
tgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaa
cggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgacc
aagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcacc
ccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacgg
cggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaac
ttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacg
gcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccaccta
caaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggac
tacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataat
actagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtga
acgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttatgacaacttga
cggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccg
cgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagc
ttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagac
gcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactg
acaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgc
tcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgc
tgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatg
ccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatc
tctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgc
gaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaat
cggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatactt
ttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggc
tcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaac
gcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccata
gcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttt
tttgggctagctactagagaactagaatcacctcttgcttttgggtaagacagaagaggagatactagatgagcacaaa
aaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaactt
ggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatg
cattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccag
agaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcat
gttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaa
aagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcc
tgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgat
gagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcc
catgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacga
cgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaac
gaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactgg
ctcaccttcgggtgggcctttctgcgtttatatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattc
attaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcac
tcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcac
acatactagagacccaaaagcaagaggtgattctagttggtggttaatgaaaattaacttacttactagaaatatctct
aaaaagccagattattaatccggctttactagagcgggcgggcgggcgggcgggatccataaaaaaaaaacccaaaagc
aagaggtgattctagttaaaaaaaaaaagcttcccgcccgcccgcccgcccgtactagagccaggcatcaaataaaacg
aaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggc
tcaccttcgggtgggcctttctgcgtttata