LOCUS BBa_K235027 2490 bp DNA linear 25-Nov-2024 DEFINITION [K235024][K235000] (NAND gate control test, arabinose positive control) ACCESSION BBa_K235027 VERSION BBa_K235027.1 FEATURES Location/Qualifiers promoter 38..43 /label=-10 promoter 15..20 /label=-35 misc_feature 25..41 /label=OR1 misc_feature 1..17 /label=OR2 misc_feature 1..49 /label=BBa_R0051 promoter 1..49 /label=BBa_R0051 misc_feature 62..65 /label=conserved RBS 58..69 /label=BBa_B0034 CDS 76..786 /label=mCherry misc_feature 427..427 /label=C->T (removing PstI site) misc_feature 76..789 /label=BBa_J06504 stem_loop 809..852 /label=stem_loop misc_feature 798..877 /label=BBa_B0010 terminator 798..877 /label=BBa_B0010 stem_loop 893..912 /label=T7 TE misc_feature 919..919 /label=stop polyA_site 913..926 /label=polya misc_feature 886..926 /label=BBa_B0012 terminator 886..926 /label=BBa_B0012 misc_feature 1..926 /label=BBa_K235018 promoter 935..969 /label=BBa_J23102 misc_feature 978..1020 /label=BBa_J23032 misc_feature 935..1020 /label=BBa_K235009 CDS 1030..1737 /label=cI lambda misc_feature 1738..1770 /label=LVA misc_feature 1777..1801 /label=Help:Barcodes CDS 1027..1801 /label=BBa_C0051 stem_loop 1821..1864 /label=stem_loop misc_feature 1810..1889 /label=BBa_B0010 terminator 1810..1889 /label=BBa_B0010 stem_loop 1905..1924 /label=T7 TE misc_feature 1931..1931 /label=stop polyA_site 1925..1938 /label=polya misc_feature 1898..1938 /label=BBa_B0012 terminator 1898..1938 /label=BBa_B0012 terminator 1810..1938 /label=BBa_B0015 misc_feature 1027..1938 /label=BBa_K235003 misc_feature 935..1938 /label=BBa_K235021 misc_feature 1..1938 /label=BBa_K235024 CDS 1947..2034 /label=end of LacI coding region (inactive) misc_feature 1947..2146 /label=BBa_R0010 misc_feature 2035..2072 /label=CAP binding site promoter 2083..2088 /label=-35 promoter 2107..2112 /label=-10 misc_feature 2112..2146 /label=LacI binding site misc_feature 2119..2119 /label=start promoter 1947..2146 /label=BBa_R0010 misc_feature 2155..2248 /label=BBa_J23008 misc_feature 2257..2353 /label=BBa_J23009 stem_loop 2373..2416 /label=stem_loop misc_feature 2362..2441 /label=BBa_B0010 terminator 2362..2441 /label=BBa_B0010 stem_loop 2457..2476 /label=T7 TE misc_feature 2483..2483 /label=stop polyA_site 2477..2490 /label=polya misc_feature 2450..2490 /label=BBa_B0012 terminator 2450..2490 /label=BBa_B0012 misc_feature 1947..2490 /label=BBa_K235000 ORIGIN 1 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgct actagagaaa 61 gaggagaaat actagatggt gagcaagggc gaggaggata acatggccat catcaaggag 121 ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag 181 ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag 241 ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag 301 gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc 361 ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac 421 tcctccttgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc 481 tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg 541 taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc 601 ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc 661 ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc 721 gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac 781 aagtaataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc 841 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc 901 ttcgggtggg cctttctgcg tttatatact agagttgaca gctagctcag tcctaggtac 961 tgtgctagct actagagaac tagaatcacc tcttgctttt gggtaagaca gaagaggaga 1021 tactagatga gcacaaaaaa gaaaccatta acacaagagc agcttgagga cgcacgtcgc 1081 cttaaagcaa tttatgaaaa aaagaaaaat gaacttggct tatcccagga atctgtcgca 1141 gacaagatgg ggatggggca gtcaggcgtt ggtgctttat ttaatggcat caatgcatta 1201 aatgcttata acgccgcatt gcttgcaaaa attctcaaag ttagcgttga agaatttagc 1261 ccttcaatcg ccagagaaat ctacgagatg tatgaagcgg ttagtatgca gccgtcactt 1321 agaagtgagt atgagtaccc tgttttttct catgttcagg cagggatgtt ctcacctgag 1381 cttagaacct ttaccaaagg tgatgcggag agatgggtaa gcacaaccaa aaaagccagt 1441 gattctgcat tctggcttga ggttgaaggt aattccatga ccgcaccaac aggctccaag 1501 ccaagctttc ctgacggaat gttaattctc gttgaccctg agcaggctgt tgagccaggt 1561 gatttctgca tagccagact tgggggtgat gagtttacct tcaagaaact gatcagggat 1621 agcggtcagg tgtttttaca accactaaac ccacagtacc caatgatccc atgcaatgag 1681 agttgttccg ttgtggggaa agttatcgct agtcagtggc ctgaagagac gtttggcgct 1741 gcaaacgacg aaaactacgc tttagtagct taataacgct gatagtgcta gtgtagatcg 1801 ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt 1861 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg 1921 ggcctttctg cgtttatata ctagagcaat acgcaaaccg cctctccccg cgcgttggcc 1981 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa 2041 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc 2101 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacatact agagacccaa 2161 aagcaagagg tgattctagt tggtggttaa tgaaaattaa cttacttact agaaatatct 2221 ctaaaaagcc agattattaa tccggcttta ctagagcggg cgggcgggcg ggcgggatcc 2281 ataaaaaaaa aacccaaaag caagaggtga ttctagttaa aaaaaaaaag cttcccgccc 2341 gcccgcccgc ccgtactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact 2401 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct 2461 caccttcggg tgggcctttc tgcgtttata //