##gff-version 3
##sequence-region BBa_K235027 1 2490
BBa_K235027	.	engineered_region	1947	2490	.	+	0	ID=BBa_K235000;Name=BBa_K235000
BBa_K235027	.	mature_transcript_region	2257	2353	.	+	0	ID=BBa_J23009;Name=BBa_J23009;Parent=BBa_K235000
BBa_K235027	.	mature_transcript_region	2155	2248	.	+	0	ID=BBa_J23008;Name=BBa_J23008;Parent=BBa_K235000
BBa_K235027	.	promoter	1947	2146	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_K235000
BBa_K235027	.	non_covalent_binding_site	2035	2072	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K235027	.	promoter	2083	2088	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K235027	.	CDS	1947	2034	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K235027	.	start_codon	2119	2119	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K235027	.	promoter	2107	2112	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K235027	.	non_covalent_binding_site	2112	2146	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K235027	.	engineered_region	1947	2146	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K235027	.	terminator	2450	2490	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K235000
BBa_K235027	.	polyA_site	2477	2490	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K235027	.	stem_loop	2457	2476	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K235027	.	stop_codon	2483	2483	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K235027	.	engineered_region	2450	2490	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K235027	.	terminator	2362	2441	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K235000
BBa_K235027	.	stem_loop	2373	2416	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K235027	.	engineered_region	2362	2441	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K235027	.	engineered_region	1	1938	.	+	0	ID=BBa_K235024;Name=BBa_K235024
BBa_K235027	.	engineered_region	1	926	.	+	0	ID=BBa_K235018;Name=BBa_K235018;Parent=BBa_K235024
BBa_K235027	.	terminator	798	877	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K235018
BBa_K235027	.	stem_loop	809	852	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K235027	.	engineered_region	798	877	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K235027	.	ribosome_entry_site	58	69	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K235018
BBa_K235027	.	conserved_region	62	65	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K235027	.	terminator	886	926	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K235018
BBa_K235027	.	polyA_site	913	926	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K235027	.	stem_loop	893	912	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K235027	.	stop_codon	919	919	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K235027	.	engineered_region	886	926	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K235027	.	engineered_region	76	789	.	+	0	ID=BBa_J06504;Name=BBa_J06504;Parent=BBa_K235018
BBa_K235027	.	CDS	76	786	.	+	0	ID=annotation1585829;Name=mCherry;Parent=BBa_J06504
BBa_K235027	.	sequence_alteration	427	427	.	+	0	ID=annotation1585833;Name=C->T (removing PstI site);Parent=BBa_J06504
BBa_K235027	.	promoter	1	49	.	+	0	ID=BBa_R0051;Name=BBa_R0051;Parent=BBa_K235018
BBa_K235027	.	engineered_region	1	49	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_K235027	.	non_covalent_binding_site	25	41	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_K235027	.	non_covalent_binding_site	1	17	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_K235027	.	promoter	15	20	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_K235027	.	promoter	38	43	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_K235027	.	engineered_region	935	1938	.	+	0	ID=BBa_K235021;Name=BBa_K235021;Parent=BBa_K235024
BBa_K235027	.	engineered_region	935	1020	.	+	0	ID=BBa_K235009;Name=BBa_K235009;Parent=BBa_K235021
BBa_K235027	.	mature_transcript_region	978	1020	.	+	0	ID=BBa_J23032;Name=BBa_J23032;Parent=BBa_K235009
BBa_K235027	.	promoter	935	969	.	+	0	ID=BBa_J23102;Name=BBa_J23102;Parent=BBa_K235009
BBa_K235027	.	engineered_region	1027	1938	.	+	0	ID=BBa_K235003;Name=BBa_K235003;Parent=BBa_K235021
BBa_K235027	.	terminator	1810	1938	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K235003
BBa_K235027	.	terminator	1898	1938	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K235027	.	polyA_site	1925	1938	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K235027	.	stem_loop	1905	1924	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K235027	.	stop_codon	1931	1931	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K235027	.	engineered_region	1898	1938	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K235027	.	terminator	1810	1889	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K235027	.	stem_loop	1821	1864	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K235027	.	engineered_region	1810	1889	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K235027	.	CDS	1027	1801	.	+	0	ID=BBa_C0051;Name=BBa_C0051;Parent=BBa_K235003
BBa_K235027	.	engineered_tag	1777	1801	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_K235027	.	tag	1738	1770	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_K235027	.	CDS	1030	1737	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
>BBa_K235027
taacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagaaagaggagaaatactagatgg
tgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaa
cggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgacc
aagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcacc
ccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacgg
cggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaac
ttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacg
gcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccaccta
caaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggac
tacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataat
actagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtga
acgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacagctagctc
agtcctaggtactgtgctagctactagagaactagaatcacctcttgcttttgggtaagacagaagaggagatactaga
tgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaa
aaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaat
ggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagccctt
caatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgt
tttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagc
acaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagc
caagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagact
tgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtac
ccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcg
ctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcat
caaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactaga
gtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcaatacgcaaaccgcctctccccgcgcgt
tggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtga
gttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggata
acaatttcacacatactagagacccaaaagcaagaggtgattctagttggtggttaatgaaaattaacttacttactag
aaatatctctaaaaagccagattattaatccggctttactagagcgggcgggcgggcgggcgggatccataaaaaaaaa
acccaaaagcaagaggtgattctagttaaaaaaaaaaagcttcccgcccgcccgcccgcccgtactagagccaggcatc
aaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagag
tcacactggctcaccttcgggtgggcctttctgcgtttata