BBa_K235027
type: ComponentDefinition
persistent identity: BBa K235027
display id: BBa_K235027
version: 1
was generated by: iGEM to SBOL conversion
description: [K235024][K235000] (NAND gate control test, arabinose positive control)
created: 2009-09-02T11:00:00Z
modified: 2015-05-08T01:11:36Z
mutable provenance: K235024+K235000
owned by: james
owned by: myers
mutable description: This BioBrick is a control for the NAND gate (K235025). This system constitutively produces the ribolocked mRNA for the λcI repressor protein. It can therefore be used as a positive control for the arabinose segment of the system; only the presence or absence of IPTG should change the level of mCherry.
discontinued: false
dominant: false
group u list: _336_
m user id: 0
owner id: 5167
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Constructed using three-antibiotic assembly. On plasmid pSB1A3.
star: false
creator: Kyliah Clarkson
type: DnaRegion
role: partType/Project
role: SO:0000804
component: BBa_K235024
component: BBa_K235000
sequence annotation: BBa_K235024
sequence annotation: BBa_K235000
sequence: https://synbiohub.org/public/igem/BBa_K235027_sequence/1

BBa_K235024
type: Component
persistent identity: component2307260
display id: component2307260
version: 1
top level: BBa_K235027
definition: BBa_K235024
access: public

BBa_K235000
type: Component
persistent identity: component2307276
display id: component2307276
version: 1
top level: BBa_K235027
definition: BBa_K235000
access: public

BBa_K235024
type: SequenceAnnotation
persistent identity: annotation2307260
display id: annotation2307260
version: 1
top level: BBa_K235027
location: https://synbiohub.org/public/igem/BBa_K235027/annotation2307260/range2307260/1
component: BBa_K235024

https://synbiohub.org/public/igem/BBa_K235027/annotation2307260/range2307260/1
type: Range
persistent identity: range2307260
display id: range2307260
version: 1
top level: BBa_K235027
direction: direction/reverse
start: 1
end: 1938
orientation: inline

BBa_K235000
type: SequenceAnnotation
persistent identity: annotation2307276
display id: annotation2307276
version: 1
top level: BBa_K235027
location: https://synbiohub.org/public/igem/BBa_K235027/annotation2307276/range2307276/1
component: BBa_K235000

https://synbiohub.org/public/igem/BBa_K235027/annotation2307276/range2307276/1
type: Range
persistent identity: range2307276
display id: range2307276
version: 1
top level: BBa_K235027
direction: direction/reverse
start: 1947
end: 2490
orientation: inline

BBa_K235000
type: ComponentDefinition
persistent identity: BBa K235000
display id: BBa_K235000
version: 1
was generated by: iGEM to SBOL conversion
description: [R0010][J23066] (pLac+ribokey+stop)
created: 2009-09-02T11:00:00Z
modified: 2015-05-08T01:11:35Z
mutable provenance: R0010+J23066
owned by: james
owned by: myers
mutable description: This part generates a ribokey (J23066) under the control of the lactose promoter. The key will not be produced except in LacI-deficient strains, or when cultured with lactose or inducers such as IPTG.
discontinued: false
dominant: false
experience: experience/None
group u list: _336_
m user id: 0
owner id: 5167
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Constructed using the three-antibiotic assembly method, is on plasmid pSB1C3
star: false
creator: Kyliah Clarkson
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_J23008
component: BBa_R0010
component: BBa_B0010
component: BBa_J23009
component: BBa_B0012
sequence annotation: BBa_R0010
sequence annotation: BBa_J23008
sequence annotation: BBa_J23009
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K235000_sequence/1

BBa_J23008
type: Component
persistent identity: component2018302
display id: component2018302
version: 1
top level: BBa_K235000
definition: BBa_J23008
access: public

BBa_R0010
type: Component
persistent identity: component2018295
display id: component2018295
version: 1
top level: BBa_K235000
definition: LacI
access: public

BBa_B0010
type: Component
persistent identity: component2018304
display id: component2018304
version: 1
top level: BBa_K235000
definition: BBa_B0010
access: public

BBa_J23009
type: Component
persistent identity: component2018303
display id: component2018303
version: 1
top level: BBa_K235000
definition: BBa_J23009
access: public

BBa_B0012
type: Component
persistent identity: component2018306
display id: component2018306
version: 1
top level: BBa_K235000
definition: BBa_B0012
access: public

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation2018295
display id: annotation2018295
version: 1
top level: BBa_K235000
location: https://synbiohub.org/public/igem/BBa_K235000/annotation2018295/range2018295/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_K235000/annotation2018295/range2018295/1
type: Range
persistent identity: range2018295
display id: range2018295
version: 1
top level: BBa_K235000
direction: direction/forward
start: 1
end: 200
orientation: inline

BBa_J23008
type: SequenceAnnotation
persistent identity: annotation2018302
display id: annotation2018302
version: 1
top level: BBa_K235000
location: https://synbiohub.org/public/igem/BBa_K235000/annotation2018302/range2018302/1
component: BBa_J23008

https://synbiohub.org/public/igem/BBa_K235000/annotation2018302/range2018302/1
type: Range
persistent identity: range2018302
display id: range2018302
version: 1
top level: BBa_K235000
direction: direction/reverse
start: 209
end: 302
orientation: inline

BBa_J23009
type: SequenceAnnotation
persistent identity: annotation2018303
display id: annotation2018303
version: 1
top level: BBa_K235000
location: https://synbiohub.org/public/igem/BBa_K235000/annotation2018303/range2018303/1
component: BBa_J23009

https://synbiohub.org/public/igem/BBa_K235000/annotation2018303/range2018303/1
type: Range
persistent identity: range2018303
display id: range2018303
version: 1
top level: BBa_K235000
direction: direction/reverse
start: 311
end: 407
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2018304
display id: annotation2018304
version: 1
top level: BBa_K235000
location: https://synbiohub.org/public/igem/BBa_K235000/annotation2018304/range2018304/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K235000/annotation2018304/range2018304/1
type: Range
persistent identity: range2018304
display id: range2018304
version: 1
top level: BBa_K235000
direction: direction/forward
start: 416
end: 495
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2018306
display id: annotation2018306
version: 1
top level: BBa_K235000
location: https://synbiohub.org/public/igem/BBa_K235000/annotation2018306/range2018306/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K235000/annotation2018306/range2018306/1
type: Range
persistent identity: range2018306
display id: range2018306
version: 1
top level: BBa_K235000
direction: direction/forward
start: 504
end: 544
orientation: inline

BBa_K235024
type: ComponentDefinition
persistent identity: BBa K235024
display id: BBa_K235024
version: 1
was generated by: iGEM to SBOL conversion
description: [K235018][K235021] (mCherry generator, ribokey-mediated signal inversion)
created: 2009-09-02T11:00:00Z
modified: 2015-05-08T01:11:36Z
mutable provenance: K235018+K235021
owned by: james
owned by: myers
mutable description: This Biobrick will produce mCherry fluorescent protein (J06504) under the control of the λcI promoter. It will also constitutively produce mRNA for the λcI repressor protein (C0051). The protein will only be translated when a ribokey (i.e., J23066) is also present in the cell. This system is sensitive to the cellular production of λcI repressor protein.
discontinued: false
dominant: false
group u list: _336_
m user id: 0
owner id: 5167
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Constructed using three-antibiotic assembly. On plasmid pSB1C3.
star: false
creator: Kyliah Clarkson
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_K235021
component: BBa_K235018
sequence annotation: BBa_K235018
sequence annotation: BBa_K235021
sequence: https://synbiohub.org/public/igem/BBa_K235024_sequence/1

BBa_K235021
type: Component
persistent identity: component2284893
display id: component2284893
version: 1
top level: BBa_K235024
definition: BBa_K235021
access: public

BBa_K235018
type: Component
persistent identity: component2284877
display id: component2284877
version: 1
top level: BBa_K235024
definition: BBa_K235018
access: public

BBa_K235018
type: SequenceAnnotation
persistent identity: annotation2284877
display id: annotation2284877
version: 1
top level: BBa_K235024
location: https://synbiohub.org/public/igem/BBa_K235024/annotation2284877/range2284877/1
component: BBa_K235018

https://synbiohub.org/public/igem/BBa_K235024/annotation2284877/range2284877/1
type: Range
persistent identity: range2284877
display id: range2284877
version: 1
top level: BBa_K235024
direction: direction/reverse
start: 1
end: 926
orientation: inline

BBa_K235021
type: SequenceAnnotation
persistent identity: annotation2284893
display id: annotation2284893
version: 1
top level: BBa_K235024
location: https://synbiohub.org/public/igem/BBa_K235024/annotation2284893/range2284893/1
component: BBa_K235021

https://synbiohub.org/public/igem/BBa_K235024/annotation2284893/range2284893/1
type: Range
persistent identity: range2284893
display id: range2284893
version: 1
top level: BBa_K235024
direction: direction/reverse
start: 935
end: 1938
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
star: true
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_R0010
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 88
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 200
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
top level: LacI
direction: direction/forward
start: 89
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
top level: LacI
direction: direction/forward
start: 137
end: 142
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
top level: LacI
direction: direction/forward
start: 161
end: 166
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
top level: LacI
direction: direction/forward
start: 166
end: 200
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
top level: LacI
direction: direction/forward
start: 173
end: 173
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_J23008
type: ComponentDefinition
persistent identity: BBa J23008
display id: BBa_J23008
version: 1
was generated by: iGEM to SBOL conversion
description: [key3c] riboregulator for lock3 variants
created: 2006-07-09T11:00:00Z
modified: 2015-08-31T04:08:38Z
mutable provenance: J23007
owned by: james
owned by: myers
mutable description: This part has the same homology region to J01122 as J23007 except that unlike J23007, J23008 does not contain the hairpin that was derived from J01129. J23008 anneals linearly to the stem of the hairpin in J01122 with perfect base pairing and destroys the hairpin in the process and reveals the RBS on J01122. When J01122 is transcribed the RNA secondary structure is such that the RBS hairpins with sequence upstream preventing the ribosome from binding and translation from occuring thereby locking up RFP further down stream. When J23008 is introduced into the cell in trans, we suspect that it will unlock the RBS for RFP as described above allowing translation to occur.
discontinued: false
dominant: false
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 931
owning group id: 52
sample status: In stock
status: status/Available
bookmark: false
mutable notes: No design considerations
star: true
creator: Kaitlin Davis
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence: https://synbiohub.org/public/igem/BBa_J23008_sequence/1

BBa_J23009
type: ComponentDefinition
persistent identity: BBa J23009
display id: BBa_J23009
version: 1
was generated by: iGEM to SBOL conversion
description: [key3d] riboregulator for lock3 variants
created: 2006-08-02T11:00:00Z
modified: 2015-08-31T04:08:38Z
mutable provenance: Overlap extension
owned by: james
owned by: myers
mutable description: Variant of key3 (see part J01129 (key3), J23007(key3b), J23008(key3c)). Part is in pJ23006, so there is a Ptet upstream of the XbaI site to drive expression. Nevertheless, J23009 is still a basic part allowing both prefix and suffix insertions.
discontinued: false
dominant: false
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence: https://synbiohub.org/public/igem/BBa_J23009_sequence/1

BBa_K235018
type: ComponentDefinition
persistent identity: BBa K235018
display id: BBa_K235018
version: 1
was generated by: iGEM to SBOL conversion
description: [K235015][K235005] pLambda-controlled mCherry generator
created: 2009-09-02T11:00:00Z
modified: 2015-05-08T01:11:36Z
mutable provenance: K235015+K235005
owned by: james
owned by: myers
mutable description: This BioBrick constitutively generates the mCherry fluorescent protein (J06504) unless the λcI repressor protein (i.e., C0051) is present in the cell.
discontinued: false
dominant: false
group u list: _336_
m user id: 0
owner id: 5167
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Constructed using three-antibiotic assembly. On plasmid pSB1A3.
star: false
creator: Kyliah Clarkson
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0034
component: BBa_R0051
component: BBa_B0010
component: BBa_J06504
component: BBa_B0012
sequence annotation: BBa_R0051
sequence annotation: BBa_B0034
sequence annotation: BBa_J06504
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K235018_sequence/1

BBa_B0034
type: Component
persistent identity: component2018462
display id: component2018462
version: 1
top level: BBa_K235018
definition: BBa_B0034
access: public

BBa_R0051
type: Component
persistent identity: component2018457
display id: component2018457
version: 1
top level: BBa_K235018
definition: cI lam
access: public

BBa_B0010
type: Component
persistent identity: component2018466
display id: component2018466
version: 1
top level: BBa_K235018
definition: BBa_B0010
access: public

BBa_J06504
type: Component
persistent identity: component2018465
display id: component2018465
version: 1
top level: BBa_K235018
definition: mCherry
access: public

BBa_B0012
type: Component
persistent identity: component2018468
display id: component2018468
version: 1
top level: BBa_K235018
definition: BBa_B0012
access: public

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation2018457
display id: annotation2018457
version: 1
top level: BBa_K235018
location: https://synbiohub.org/public/igem/BBa_K235018/annotation2018457/range2018457/1
component: BBa_R0051

https://synbiohub.org/public/igem/BBa_K235018/annotation2018457/range2018457/1
type: Range
persistent identity: range2018457
display id: range2018457
version: 1
top level: BBa_K235018
direction: direction/forward
start: 1
end: 49
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2018462
display id: annotation2018462
version: 1
top level: BBa_K235018
location: https://synbiohub.org/public/igem/BBa_K235018/annotation2018462/range2018462/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K235018/annotation2018462/range2018462/1
type: Range
persistent identity: range2018462
display id: range2018462
version: 1
top level: BBa_K235018
direction: direction/reverse
start: 58
end: 69
orientation: inline

BBa_J06504
type: SequenceAnnotation
persistent identity: annotation2018465
display id: annotation2018465
version: 1
top level: BBa_K235018
location: https://synbiohub.org/public/igem/BBa_K235018/annotation2018465/range2018465/1
component: BBa_J06504

https://synbiohub.org/public/igem/BBa_K235018/annotation2018465/range2018465/1
type: Range
persistent identity: range2018465
display id: range2018465
version: 1
top level: BBa_K235018
direction: direction/reverse
start: 76
end: 789
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2018466
display id: annotation2018466
version: 1
top level: BBa_K235018
location: https://synbiohub.org/public/igem/BBa_K235018/annotation2018466/range2018466/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K235018/annotation2018466/range2018466/1
type: Range
persistent identity: range2018466
display id: range2018466
version: 1
top level: BBa_K235018
direction: direction/forward
start: 798
end: 877
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2018468
display id: annotation2018468
version: 1
top level: BBa_K235018
location: https://synbiohub.org/public/igem/BBa_K235018/annotation2018468/range2018468/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K235018/annotation2018468/range2018468/1
type: Range
persistent identity: range2018468
display id: range2018468
version: 1
top level: BBa_K235018
direction: direction/forward
start: 886
end: 926
orientation: inline

BBa_K235021
type: ComponentDefinition
persistent identity: BBa K235021
display id: BBa_K235021
version: 1
was generated by: iGEM to SBOL conversion
description: [K235009][K235003] (ribokey-mediated lambda repressor generator)
created: 2009-09-02T11:00:00Z
modified: 2015-05-08T01:11:36Z
mutable provenance: K235009+K235003
owned by: james
owned by: myers
mutable description: This BioBrick constitutively produced mRNA for the λcI repressor protein. It will not be translated unless a ribokey (i.e., J23066) is also present in the cell.
discontinued: false
dominant: false
group u list: _336_
m user id: 0
owner id: 5167
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Constructed using three-antibiotic assembly. On plasmid pSB1A3.
star: false
creator: Kyliah Clarkson
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K235009
component: BBa_K235003
sequence annotation: BBa_K235009
sequence annotation: BBa_K235003
sequence: https://synbiohub.org/public/igem/BBa_K235021_sequence/1

BBa_K235009
type: Component
persistent identity: component2255595
display id: component2255595
version: 1
top level: BBa_K235021
definition: BBa_K235009
access: public

BBa_K235003
type: Component
persistent identity: component2255607
display id: component2255607
version: 1
top level: BBa_K235021
definition: BBa_K235003
access: public

BBa_K235009
type: SequenceAnnotation
persistent identity: annotation2255595
display id: annotation2255595
version: 1
top level: BBa_K235021
location: https://synbiohub.org/public/igem/BBa_K235021/annotation2255595/range2255595/1
component: BBa_K235009

https://synbiohub.org/public/igem/BBa_K235021/annotation2255595/range2255595/1
type: Range
persistent identity: range2255595
display id: range2255595
version: 1
top level: BBa_K235021
direction: direction/reverse
start: 1
end: 86
orientation: inline

BBa_K235003
type: SequenceAnnotation
persistent identity: annotation2255607
display id: annotation2255607
version: 1
top level: BBa_K235021
location: https://synbiohub.org/public/igem/BBa_K235021/annotation2255607/range2255607/1
component: BBa_K235003

https://synbiohub.org/public/igem/BBa_K235021/annotation2255607/range2255607/1
type: Range
persistent identity: range2255607
display id: range2255607
version: 1
top level: BBa_K235021
direction: direction/reverse
start: 93
end: 1004
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

cI lam
type: ComponentDefinition
persistent identity: BBa R0051
display id: BBa_R0051
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lambda cI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The cI regulated promoter is based on the pR promtoer from bacteriohage lambda. The promoter has two two DNA binding sites for lambda cI repressor <bb_part>BBa_C0051</bb_part>. cI binding results in repression of transcription. The specific sequence used here is based on the cI repressible promoter used in the Elowitz repressilator (and references therein).
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In order to address concerns about the promoter transcribing in the reverse direction, we have removed the -35 and -10 signals responsible for the promoter activity in the reverse direction. (More details needed here! DE, 2/24/03) Incompatible with host expressing cI repressor.
star: true
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: BBa_R0051
sequence annotation: OR2
sequence annotation: -35
sequence annotation: OR1
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0051_sequence/1

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation7067
display id: annotation7067
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation7067/range7067/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_R0051/annotation7067/range7067/1
type: Range
persistent identity: range7067
display id: range7067
version: 1
top level: cI lam
direction: direction/forward
start: 1
end: 49
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2025
display id: annotation2025
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2025/range2025/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0051/annotation2025/range2025/1
type: Range
persistent identity: range2025
display id: range2025
version: 1
top level: cI lam
direction: direction/forward
start: 1
end: 17
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2023
display id: annotation2023
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2023/range2023/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0051/annotation2023/range2023/1
type: Range
persistent identity: range2023
display id: range2023
version: 1
top level: cI lam
direction: direction/forward
start: 15
end: 20
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation2024
display id: annotation2024
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2024/range2024/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0051/annotation2024/range2024/1
type: Range
persistent identity: range2024
display id: range2024
version: 1
top level: cI lam
direction: direction/forward
start: 25
end: 41
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2022
display id: annotation2022
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2022/range2022/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0051/annotation2022/range2022/1
type: Range
persistent identity: range2022
display id: range2022
version: 1
top level: cI lam
direction: direction/forward
start: 38
end: 43
orientation: inline

mCherry
type: ComponentDefinition
persistent identity: BBa J06504
display id: BBa_J06504
version: 1
was generated by: iGEM to SBOL conversion
description: monomeric RFP optimized for bacteria
created: 2005-07-17T11:00:00Z
modified: 2016-01-25T01:05:28Z
mutable provenance: mCherry from Roger Y. Tsien's lab, altered to be BioBrick compatible
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: mRFP (DsRed) derived, altered to be a biobrick by removing a PstI site and adding in the ends.
discontinued: false
dominant: false
experience: experience/None
group u list: _20_
m user id: 4206
owner id: 340
owning group id: 20
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Made a point mutation to eliminate a PstI site in the middle and then added BioBrick ends using PCR. Sequenced using primers that bind to the pSB1A2 plasmid on either side of the brick.
star: true
creator: ytwang
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: mCherry
sequence annotation: C->T (removing PstI site)
sequence: https://synbiohub.org/public/igem/BBa_J06504_sequence/1

mCherry
type: SequenceAnnotation
persistent identity: annotation1585829
display id: annotation1585829
version: 1
top level: mCherry
location: https://synbiohub.org/public/igem/BBa_J06504/annotation1585829/range1585829/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_J06504/annotation1585829/range1585829/1
type: Range
persistent identity: range1585829
display id: range1585829
version: 1
top level: mCherry
direction: direction/forward
start: 1
end: 711
orientation: inline

C->T (removing PstI site)
type: SequenceAnnotation
persistent identity: annotation1585833
display id: annotation1585833
version: 1
top level: mCherry
location: https://synbiohub.org/public/igem/BBa_J06504/annotation1585833/range1585833/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_J06504/annotation1585833/range1585833/1
type: Range
persistent identity: range1585833
display id: range1585833
version: 1
top level: mCherry
direction: direction/forward
start: 352
end: 352
orientation: inline

BBa_K235003
type: ComponentDefinition
persistent identity: BBa K235003
display id: BBa_K235003
version: 1
was generated by: iGEM to SBOL conversion
description: [C0051][B0015] (lambda cI CDS+stop)
created: 2009-09-02T11:00:00Z
modified: 2015-05-08T01:11:35Z
mutable provenance: C0051+B0015
owned by: james
owned by: myers
mutable description: Just an intermediate part
discontinued: false
dominant: false
group u list: _336_
m user id: 0
owner id: 5167
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Constructed using three-antibiotic assembly. On plasmid pSB1C3.
star: false
creator: Kyliah Clarkson
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_C0051
component: BBa_B0015
sequence annotation: BBa_C0051
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K235003_sequence/1

BBa_C0051
type: Component
persistent identity: component2246557
display id: component2246557
version: 1
top level: BBa_K235003
definition: cI lam
access: public

BBa_B0015
type: Component
persistent identity: component2246564
display id: component2246564
version: 1
top level: BBa_K235003
definition: BBa_B0015
access: public

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation2246557
display id: annotation2246557
version: 1
top level: BBa_K235003
location: https://synbiohub.org/public/igem/BBa_K235003/annotation2246557/range2246557/1
component: BBa_C0051

https://synbiohub.org/public/igem/BBa_K235003/annotation2246557/range2246557/1
type: Range
persistent identity: range2246557
display id: range2246557
version: 1
top level: BBa_K235003
direction: direction/reverse
start: 1
end: 775
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2246564
display id: annotation2246564
version: 1
top level: BBa_K235003
location: https://synbiohub.org/public/igem/BBa_K235003/annotation2246564/range2246564/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K235003/annotation2246564/range2246564/1
type: Range
persistent identity: range2246564
display id: range2246564
version: 1
top level: BBa_K235003
direction: direction/reverse
start: 784
end: 912
orientation: inline

BBa_K235009
type: ComponentDefinition
persistent identity: BBa K235009
display id: BBa_K235009
version: 1
was generated by: iGEM to SBOL conversion
description: [J23102][J23032] (constitutive promoter+ribolocked RBS)
created: 2009-09-02T11:00:00Z
modified: 2015-05-08T01:11:35Z
mutable provenance: J23102+J23032
owned by: james
owned by: myers
mutable description: Just an intermediate part.
discontinued: false
dominant: false
group u list: _336_
m user id: 0
owner id: 5167
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Constructed using three-antibiotic assembly. On plasmid pSB1C3.
star: false
creator: Kyliah Clarkson
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_J23032
component: BBa_J23102
sequence annotation: BBa_J23102
sequence annotation: BBa_J23032
sequence: https://synbiohub.org/public/igem/BBa_K235009_sequence/1

BBa_J23032
type: Component
persistent identity: component2018372
display id: component2018372
version: 1
top level: BBa_K235009
definition: BBa_J23032
access: public

BBa_J23102
type: Component
persistent identity: component2018371
display id: component2018371
version: 1
top level: BBa_K235009
definition: BBa_J23102
access: public

BBa_J23102
type: SequenceAnnotation
persistent identity: annotation2018371
display id: annotation2018371
version: 1
top level: BBa_K235009
location: https://synbiohub.org/public/igem/BBa_K235009/annotation2018371/range2018371/1
component: BBa_J23102

https://synbiohub.org/public/igem/BBa_K235009/annotation2018371/range2018371/1
type: Range
persistent identity: range2018371
display id: range2018371
version: 1
top level: BBa_K235009
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_J23032
type: SequenceAnnotation
persistent identity: annotation2018372
display id: annotation2018372
version: 1
top level: BBa_K235009
location: https://synbiohub.org/public/igem/BBa_K235009/annotation2018372/range2018372/1
component: BBa_J23032

https://synbiohub.org/public/igem/BBa_K235009/annotation2018372/range2018372/1
type: Range
persistent identity: range2018372
display id: range2018372
version: 1
top level: BBa_K235009
direction: direction/reverse
start: 44
end: 86
orientation: inline

cI lam
type: ComponentDefinition
persistent identity: BBa C0051
display id: BBa_C0051
version: 1
was generated by: iGEM to SBOL conversion
description: cI repressor from E. coli phage lambda (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999).
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the cI repressor based on cI repressor from bacteriophage lambda modified with an LVA tail for rapid degradation of the protein. cI repressor binds to the cI regulator (BBa_R0051).
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000 Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001 References (unparsed) here: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000 Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001 BBa_C0051 cI repressor is based on the cI repressor from the Elowitz's repressilator. It has been modified to include a rapid degradation LAA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cI lambda
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0051_sequence/1

cI lambda
type: SequenceAnnotation
persistent identity: annotation23334
display id: annotation23334
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_C0051/annotation23334/range23334/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0051/annotation23334/range23334/1
type: Range
persistent identity: range23334
display id: range23334
version: 1
top level: cI lam
direction: direction/forward
start: 4
end: 711
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation23335
display id: annotation23335
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_C0051/annotation23335/range23335/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_C0051/annotation23335/range23335/1
type: Range
persistent identity: range23335
display id: range23335
version: 1
top level: cI lam
direction: direction/forward
start: 712
end: 744
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213991
display id: annotation2213991
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_C0051/annotation2213991/range2213991/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0051/annotation2213991/range2213991/1
type: Range
persistent identity: range2213991
display id: range2213991
version: 1
top level: cI lam
direction: direction/forward
start: 751
end: 775
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_J23102
type: ComponentDefinition
persistent identity: BBa J23102
display id: BBa_J23102
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: isolated from library of promoters
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: replace later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23102_sequence/1

BBa_J23032
type: ComponentDefinition
persistent identity: BBa J23032
display id: BBa_J23032
version: 1
was generated by: iGEM to SBOL conversion
description: [lock3d]
created: 2006-08-02T11:00:00Z
modified: 2015-08-31T04:08:39Z
mutable provenance: Extension of overlapping oligonucleotides
owned by: james
owned by: myers
mutable description: lock3d
discontinued: false
dominant: false
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: partType/RNA
role: SO:0000834
sequence: https://synbiohub.org/public/igem/BBa_J23032_sequence/1

https://synbiohub.org/public/igem/BBa_K235027_sequence/1
type: Sequence
persistent identity: BBa K235027 sequence
display id: BBa_K235027_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagaaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacagctagctcagtcctaggtactgtgctagctactagagaactagaatcacctcttgcttttgggtaagacagaagaggagatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K235000_sequence/1
type: Sequence
persistent identity: BBa K235000 sequence
display id: BBa_K235000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagacccaaaagcaagaggtgattctagttggtggttaatgaaaattaacttacttactagaaatatctctaaaaagccagattattaatccggctttactagagcgggcgggcgggcgggcgggatccataaaaaaaaaacccaaaagcaagaggtgattctagttaaaaaaaaaaagcttcccgcccgcccgcccgcccgtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K235024_sequence/1
type: Sequence
persistent identity: BBa K235024 sequence
display id: BBa_K235024_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagaaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacagctagctcagtcctaggtactgtgctagctactagagaactagaatcacctcttgcttttgggtaagacagaagaggagatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0010_sequence/1
type: Sequence
persistent identity: BBa R0010 sequence
display id: BBa_R0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23008_sequence/1
type: Sequence
persistent identity: BBa J23008 sequence
display id: BBa_J23008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acccaaaagcaagaggtgattctagttggtggttaatgaaaattaacttacttactagaaatatctctaaaaagccagattattaatccggctt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23009_sequence/1
type: Sequence
persistent identity: BBa J23009 sequence
display id: BBa_J23009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cgggcgggcgggcgggcgggatccataaaaaaaaaacccaaaagcaagaggtgattctagttaaaaaaaaaaagcttcccgcccgcccgcccgcccg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K235018_sequence/1
type: Sequence
persistent identity: BBa K235018 sequence
display id: BBa_K235018_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagaaagaggagaaatactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K235021_sequence/1
type: Sequence
persistent identity: BBa K235021 sequence
display id: BBa_K235021_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctaggtactgtgctagctactagagaactagaatcacctcttgcttttgggtaagacagaagaggagatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0051_sequence/1
type: Sequence
persistent identity: BBa R0051 sequence
display id: BBa_R0051_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taacaccgtgcgtgttgactattttacctctggcggtgataatggttgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J06504_sequence/1
type: Sequence
persistent identity: BBa J06504 sequence
display id: BBa_J06504_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K235003_sequence/1
type: Sequence
persistent identity: BBa K235003 sequence
display id: BBa_K235003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K235009_sequence/1
type: Sequence
persistent identity: BBa K235009 sequence
display id: BBa_K235009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctaggtactgtgctagctactagagaactagaatcacctcttgcttttgggtaagacagaagaggaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0051_sequence/1
type: Sequence
persistent identity: BBa C0051 sequence
display id: BBa_C0051_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23102_sequence/1
type: Sequence
persistent identity: BBa J23102 sequence
display id: BBa_J23102_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctaggtactgtgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23032_sequence/1
type: Sequence
persistent identity: BBa J23032 sequence
display id: BBa_J23032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aactagaatcacctcttgcttttgggtaagacagaagaggaga
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z