LOCUS       BBa_K235028             2325 bp    DNA     linear       22-Dec-2024
DEFINITION  [K235024][K235001] (NAND gate control test, positive control)
ACCESSION   BBa_K235028
VERSION     BBa_K235028.1  
FEATURES             Location/Qualifiers
     promoter        38..43
                     /label=-10
     promoter        15..20
                     /label=-35
     misc_feature    25..41
                     /label=OR1
     misc_feature    1..17
                     /label=OR2
     misc_feature    1..49
                     /label=BBa_R0051
     promoter        1..49
                     /label=BBa_R0051
     misc_feature    62..65
                     /label=conserved
     RBS             58..69
                     /label=BBa_B0034
     CDS             76..786
                     /label=mCherry
     misc_feature    427..427
                     /label=C->T (removing PstI site)
     misc_feature    76..789
                     /label=BBa_J06504
     stem_loop       809..852
                     /label=stem_loop
     misc_feature    798..877
                     /label=BBa_B0010
     terminator      798..877
                     /label=BBa_B0010
     stem_loop       893..912
                     /label=T7 TE
     misc_feature    919..919
                     /label=stop
     polyA_site      913..926
                     /label=polya
     misc_feature    886..926
                     /label=BBa_B0012
     terminator      886..926
                     /label=BBa_B0012
     misc_feature    1..926
                     /label=BBa_K235018
     promoter        935..969
                     /label=BBa_J23102
     misc_feature    978..1020
                     /label=BBa_J23032
     misc_feature    935..1020
                     /label=BBa_K235009
     CDS             1030..1737
                     /label=cI lambda
     misc_feature    1738..1770
                     /label=LVA
     misc_feature    1777..1801
                     /label=Help:Barcodes
     CDS             1027..1801
                     /label=BBa_C0051
     stem_loop       1821..1864
                     /label=stem_loop
     misc_feature    1810..1889
                     /label=BBa_B0010
     terminator      1810..1889
                     /label=BBa_B0010
     stem_loop       1905..1924
                     /label=T7 TE
     misc_feature    1931..1931
                     /label=stop
     polyA_site      1925..1938
                     /label=polya
     misc_feature    1898..1938
                     /label=BBa_B0012
     terminator      1898..1938
                     /label=BBa_B0012
     terminator      1810..1938
                     /label=BBa_B0015
     misc_feature    1027..1938
                     /label=BBa_K235003
     misc_feature    935..1938
                     /label=BBa_K235021
     misc_feature    1..1938
                     /label=BBa_K235024
     promoter        1947..1981
                     /label=BBa_J23102
     misc_feature    1990..2083
                     /label=BBa_J23008
     misc_feature    2092..2188
                     /label=BBa_J23009
     stem_loop       2208..2251
                     /label=stem_loop
     misc_feature    2197..2276
                     /label=BBa_B0010
     terminator      2197..2276
                     /label=BBa_B0010
     stem_loop       2292..2311
                     /label=T7 TE
     misc_feature    2318..2318
                     /label=stop
     polyA_site      2312..2325
                     /label=polya
     misc_feature    2285..2325
                     /label=BBa_B0012
     terminator      2285..2325
                     /label=BBa_B0012
     misc_feature    1947..2325
                     /label=BBa_K235001
ORIGIN
        1 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgct actagagaaa
       61 gaggagaaat actagatggt gagcaagggc gaggaggata acatggccat catcaaggag
      121 ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag
      181 ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag
      241 ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag
      301 gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc
      361 ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac
      421 tcctccttgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc
      481 tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg
      541 taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc
      601 ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc
      661 ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc
      721 gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac
      781 aagtaataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
      841 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc
      901 ttcgggtggg cctttctgcg tttatatact agagttgaca gctagctcag tcctaggtac
      961 tgtgctagct actagagaac tagaatcacc tcttgctttt gggtaagaca gaagaggaga
     1021 tactagatga gcacaaaaaa gaaaccatta acacaagagc agcttgagga cgcacgtcgc
     1081 cttaaagcaa tttatgaaaa aaagaaaaat gaacttggct tatcccagga atctgtcgca
     1141 gacaagatgg ggatggggca gtcaggcgtt ggtgctttat ttaatggcat caatgcatta
     1201 aatgcttata acgccgcatt gcttgcaaaa attctcaaag ttagcgttga agaatttagc
     1261 ccttcaatcg ccagagaaat ctacgagatg tatgaagcgg ttagtatgca gccgtcactt
     1321 agaagtgagt atgagtaccc tgttttttct catgttcagg cagggatgtt ctcacctgag
     1381 cttagaacct ttaccaaagg tgatgcggag agatgggtaa gcacaaccaa aaaagccagt
     1441 gattctgcat tctggcttga ggttgaaggt aattccatga ccgcaccaac aggctccaag
     1501 ccaagctttc ctgacggaat gttaattctc gttgaccctg agcaggctgt tgagccaggt
     1561 gatttctgca tagccagact tgggggtgat gagtttacct tcaagaaact gatcagggat
     1621 agcggtcagg tgtttttaca accactaaac ccacagtacc caatgatccc atgcaatgag
     1681 agttgttccg ttgtggggaa agttatcgct agtcagtggc ctgaagagac gtttggcgct
     1741 gcaaacgacg aaaactacgc tttagtagct taataacgct gatagtgcta gtgtagatcg
     1801 ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
     1861 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg
     1921 ggcctttctg cgtttatata ctagagttga cagctagctc agtcctaggt actgtgctag
     1981 ctactagaga cccaaaagca agaggtgatt ctagttggtg gttaatgaaa attaacttac
     2041 ttactagaaa tatctctaaa aagccagatt attaatccgg ctttactaga gcgggcgggc
     2101 gggcgggcgg gatccataaa aaaaaaaccc aaaagcaaga ggtgattcta gttaaaaaaa
     2161 aaaagcttcc cgcccgcccg cccgcccgta ctagagccag gcatcaaata aaacgaaagg
     2221 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
     2281 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata
//