LOCUS       BBa_K235031             1290 bp    DNA     linear       22-Dec-2024
DEFINITION  [C0012][B0015] (LacI+stop)
ACCESSION   BBa_K235031
VERSION     BBa_K235031.1  
FEATURES             Location/Qualifiers
     misc_feature    1090..1128
                     /label=LVA
     CDS             1..1128
                     /label=lacI-LVA
     misc_feature    1..1128
                     /label=BBa_C0012
     misc_feature    1129..1153
                     /label=Help:Barcodes
     CDS             1..1128
                     /label=BBa_C0012
     stem_loop       1173..1216
                     /label=stem_loop
     misc_feature    1162..1241
                     /label=BBa_B0010
     terminator      1162..1241
                     /label=BBa_B0010
     stem_loop       1257..1276
                     /label=T7 TE
     misc_feature    1283..1283
                     /label=stop
     polyA_site      1277..1290
                     /label=polya
     misc_feature    1250..1290
                     /label=BBa_B0012
     terminator      1250..1290
                     /label=BBa_B0012
     terminator      1162..1290
                     /label=BBa_B0015
ORIGIN
        1 atggtgaatg tgaaaccagt aacgttatac gatgtcgcag agtatgccgg tgtctcttat
       61 cagaccgttt cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa
      121 gtggaagcgg cgatggcgga gctgaattac attcccaacc gcgtggcaca acaactggcg
      181 ggcaaacagt cgttgctgat tggcgttgcc acctccagtc tggccctgca cgcgccgtcg
      241 caaattgtcg cggcgattaa atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg
      301 atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg tgcacaatct tctcgcgcaa
      361 cgcgtcagtg ggctgatcat taactatccg ctggatgacc aggatgccat tgctgtggaa
      421 gctgcctgca ctaatgttcc ggcgttattt cttgatgtct ctgaccagac acccatcaac
      481 agtattattt tctcccatga agacggtacg cgactgggcg tggagcatct ggtcgcattg
      541 ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt
      601 ctggctggct ggcataaata tctcactcgc aatcaaattc agccgatagc ggaacgggaa
      661 ggcgactgga gtgccatgtc cggttttcaa caaaccatgc aaatgctgaa tgagggcatc
      721 gttcccactg cgatgctggt tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt
      781 accgagtccg ggctgcgcgt tggtgcggat atctcggtag tgggatacga cgataccgaa
      841 gacagctcat gttatatccc gccgttaacc accatcaaac aggattttcg cctgctgggg
      901 caaaccagcg tggaccgctt gctgcaactc tctcagggcc aggcggtgaa gggcaatcag
      961 ctgttgcccg tctcactggt gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc
     1021 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     1081 agcgggcagg ctgcaaacga cgaaaactac gctttagtag cttaataact ctgatagtgc
     1141 tagtgtagat ctctactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact
     1201 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct
     1261 caccttcggg tgggcctttc tgcgtttata
//